Restriction map for
pF1KB7378
(Linear) (Six-base) MAPSORT of: KIBB7378.seq Check: 6106 from: 1 to: 834
KIBB7378.orf
With 182 enzymes: SgfI *
March 3, 2008 11:00 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 434 501 834
Size: 434 67 333
Fragments arranged by size:
434 333 67
AlwNI CAG_nnn'CTG
Cuts at: 0 374 834
Size: 374 460
ApaI G_GGCC'C
Cuts at: 0 655 834
Size: 655 179
AvaI C'yCGr_G
Cuts at: 0 69 163 298 834
Size: 69 94 135 536
Fragments arranged by size:
536 135 94 69
BamHI G'GATC_C
Cuts at: 0 80 834
Size: 80 754
BanI G'GyrC_C
Cuts at: 0 101 141 159 304 334 589 834
Size: 101 40 18 145 30 255 245
Fragments arranged by size:
255 245 145 101 40 30 18
BanII G_rGCy'C
Cuts at: 0 314 655 834
Size: 314 341 179
Fragments arranged by size:
341 314 179
BbeI G_GCGC'C
Cuts at: 0 105 145 163 834
Size: 105 40 18 671
Fragments arranged by size:
671 105 40 18
BclI T'GATC_A
Cuts at: 0 686 834
Size: 686 148
BglI GCCn_nnn'nGGC
Cuts at: 0 242 289 834
Size: 242 47 545
Fragments arranged by size:
545 242 47
BglII A'GATC_T
Cuts at: 0 783 834
Size: 783 51
Bme1580I G_kGCm'C
Cuts at: 0 307 592 655 834
Size: 307 285 63 179
Fragments arranged by size:
307 285 179 63
BmgBI CAC'GTC
Cuts at: 0 90 834
Size: 90 744
BmrI ACTGGGnnnn_n'
Cuts at: 0 544 834
Size: 544 290
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 622 834
Size: 622 212
BsaAI yAC'GTr
Cuts at: 0 560 834
Size: 560 274
BsaBI GATnn'nnATC
Cuts at: 0 685 834
Size: 685 149
BsaHI Gr'CG_yC
Cuts at: 0 102 142 160 834
Size: 102 40 18 674
Fragments arranged by size:
674 102 40 18
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 619 834
Size: 619 215
BseYI C'CCAG_C
Cuts at: 0 286 367 813 834
Size: 286 81 446 21
Fragments arranged by size:
446 286 81 21
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 412 834
Size: 412 422
BsiEI CG_ry'CG
Cuts at: 0 99 357 834
Size: 99 258 477
Fragments arranged by size:
477 258 99
Bsp1286I G_dGCh'C
Cuts at: 0 307 314 592 655 834
Size: 307 7 278 63 179
Fragments arranged by size:
307 278 179 63 7
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 434 501 834
Size: 434 67 333
Fragments arranged by size:
434 333 67
BsrFI r'CCGG_y
Cuts at: 0 98 122 241 834
Size: 98 24 119 593
Fragments arranged by size:
593 119 98 24
BssHII G'CGCG_C
Cuts at: 0 323 377 390 410 436 834
Size: 323 54 13 20 26 398
Fragments arranged by size:
398 323 54 26 20 13
BstAPI GCAn_nnn'nTGC
Cuts at: 0 341 834
Size: 341 493
BstEII G'GTnAC_C
Cuts at: 0 421 834
Size: 421 413
BstXI CCAn_nnnn'nTGG
Cuts at: 0 286 744 834
Size: 286 458 90
Fragments arranged by size:
458 286 90
BstYI r'GATC_y
Cuts at: 0 80 167 783 834
Size: 80 87 616 51
Fragments arranged by size:
616 87 80 51
Bsu36I CC'TnA_GG
Cuts at: 0 584 834
Size: 584 250
BtgI C'CryG_G
Cuts at: 0 671 834
Size: 671 163
EaeI y'GGCC_r
Cuts at: 0 96 124 834
Size: 96 28 710
Fragments arranged by size:
710 96 28
EagI C'GGCC_G
Cuts at: 0 96 834
Size: 96 738
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 622 834
Size: 622 212
EcoNI CCTnn'n_nnAGG
Cuts at: 0 430 700 834
Size: 430 270 134
Fragments arranged by size:
430 270 134
EcoO109I rG'GnC_Cy
Cuts at: 0 651 652 834
Size: 651 1 182
Fragments arranged by size:
651 182 1
HaeII r_GCGC'y
Cuts at: 0 105 145 163 178 252 298 834
Size: 105 40 18 15 74 46 536
Fragments arranged by size:
536 105 74 46 40 18 15
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 343 375 742 774 834
Size: 343 32 367 32 60
Fragments arranged by size:
367 343 60 32 32
KasI G'GCGC_C
Cuts at: 0 101 141 159 834
Size: 101 40 18 675
Fragments arranged by size:
675 101 40 18
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 374 834
Size: 374 460
MspA1I CmG'CkG
Cuts at: 0 27 51 63 203 286 834
Size: 27 24 12 140 83 548
Fragments arranged by size:
548 140 83 27 24 12
NaeI GCC'GGC
Cuts at: 0 100 124 243 834
Size: 100 24 119 591
Fragments arranged by size:
591 119 100 24
NarI GG'CG_CC
Cuts at: 0 102 142 160 834
Size: 102 40 18 674
Fragments arranged by size:
674 102 40 18
NgoMIV G'CCGG_C
Cuts at: 0 98 122 241 834
Size: 98 24 119 593
Fragments arranged by size:
593 119 98 24
PflMI CCAn_nnn'nTGG
Cuts at: 0 600 677 834
Size: 600 77 157
Fragments arranged by size:
600 157 77
PmlI CAC'GTG
Cuts at: 0 560 834
Size: 560 274
PspOMI G'GGCC_C
Cuts at: 0 651 834
Size: 651 183
SfoI GGC'GCC
Cuts at: 0 103 143 161 834
Size: 103 40 18 673
Fragments arranged by size:
673 103 40 18
SgrAI Cr'CCGG_yG
Cuts at: 0 241 834
Size: 241 593
SmaI CCC'GGG
Cuts at: 0 165 834
Size: 165 669
StyI C'CwwG_G
Cuts at: 0 75 834
Size: 75 759
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 607 834
Size: 607 227
Tth111I GACn'n_nGTC
Cuts at: 0 622 834
Size: 622 212
XbaI T'CTAG_A
Cuts at: 0 780 834
Size: 780 54
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 557 834
Size: 557 277
XmaI C'CCGG_G
Cuts at: 0 163 834
Size: 163 671
Enzymes that do cut:
AarI AlwNI ApaI AvaI BamHI BanI BanII BbeI
BclI BglI BglII Bme1580I BmgBI BmrI BpmI BsaAI
BsaBI BsaHI BseRI BseYI BsgI BsiEI Bsp1286I BspMI
BsrFI BssHII BstAPI BstEII BstXI BstYI Bsu36I BtgI
EaeI EagI Eco57MI EcoNI EcoO109I HaeII Hin4I KasI
MmeI MspA1I NaeI NarI NgoMIV PflMI PmlI PspOMI
SfoI SgrAI SmaI StyI TaqII Tth111I XbaI XcmI
XmaI
Enzymes that do not cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI ApaLI ApoI AscI
AseI AsiSI AvrII BaeI BbsI BbvCI BcgI BciVI
BfrBI BlpI BmtI BplI Bpu10I BpuEI BsaI BsaWI
BsaXI BsiHKAI BsiWI BsmI BsmBI BspEI BspHI BsrBI
BsrDI BsrGI BssSI BstBI BstZ17I BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EarI EciI EcoICRI EcoRI
EcoRV FalI FseI FspI FspAI HincII HindIII HpaI
KpnI MfeI MluI MscI MslI NcoI NdeI NheI
NotI NruI NsiI NspI PacI PasI PciI PfoI
PmeI PpiI PpuMI PshAI PsiI PspXI PsrI PstI
PvuI PvuII RsrII SacI SacII SalI SanDI SapI
SbfI ScaI SexAI SfcI SfiI SgfI SmlI SnaBI
SpeI SphI SrfI SspI StuI SwaI TatI TstI
XhoI XmnI ZraI