Restriction map for
pF1KB7377
(Linear) (Six-base) MAPSORT of: KIBB7377.seq Check: 5056 from: 1 to: 1347
KIBB7377.orf
With 182 enzymes: SgfI *
March 3, 2008 11:00 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 253 690 1025 1347
Size: 253 437 335 322
Fragments arranged by size:
437 335 322 253
AccI GT'mk_AC
Cuts at: 0 779 1347
Size: 779 568
AleI CACnn'nnGTG
Cuts at: 0 650 676 1347
Size: 650 26 671
Fragments arranged by size:
671 650 26
AlwNI CAG_nnn'CTG
Cuts at: 0 1053 1347
Size: 1053 294
ApaI G_GGCC'C
Cuts at: 0 52 1347
Size: 52 1295
ApaLI G'TGCA_C
Cuts at: 0 591 1347
Size: 591 756
AvaI C'yCGr_G
Cuts at: 0 870 1275 1347
Size: 870 405 72
Fragments arranged by size:
870 405 72
BamHI G'GATC_C
Cuts at: 0 193 1347
Size: 193 1154
BanI G'GyrC_C
Cuts at: 0 324 641 984 1347
Size: 324 317 343 363
Fragments arranged by size:
363 343 324 317
BanII G_rGCy'C
Cuts at: 0 52 59 920 1347
Size: 52 7 861 427
Fragments arranged by size:
861 427 52 7
BbsI GAAGACnn'nnnn_
Cuts at: 0 699 1225 1347
Size: 699 526 122
Fragments arranged by size:
699 526 122
BciVI GTATCCnnnnn_n'
Cuts at: 0 491 1347
Size: 491 856
BglII A'GATC_T
Cuts at: 0 266 1347
Size: 266 1081
Bme1580I G_kGCm'C
Cuts at: 0 52 329 595 644 1347
Size: 52 277 266 49 703
Fragments arranged by size:
703 277 266 52 49
BmgBI CAC'GTC
Cuts at: 0 546 1347
Size: 546 801
BmrI ACTGGGnnnn_n'
Cuts at: 0 525 1347
Size: 525 822
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 213 1347
Size: 213 1134
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 43 108 283 789 931 1222 1347
Size: 43 65 175 506 142 291 125
Fragments arranged by size:
506 291 175 142 125 65 43
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1020 1347
Size: 1020 327
BsaAI yAC'GTr
Cuts at: 0 606 1347
Size: 606 741
BsaHI Gr'CG_yC
Cuts at: 0 440 1347
Size: 440 907
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 808 838 1347
Size: 808 30 509
Fragments arranged by size:
808 509 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 336 928 994 1347
Size: 336 592 66 353
Fragments arranged by size:
592 353 336 66
BseYI C'CCAG_C
Cuts at: 0 45 303 820 993 1122 1339 1347
Size: 45 258 517 173 129 217 8
Fragments arranged by size:
517 258 217 173 129 45 8
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 186 1048 1347
Size: 186 862 299
Fragments arranged by size:
862 299 186
BsiHKAI G_wGCw'C
Cuts at: 0 101 595 1347
Size: 101 494 752
Fragments arranged by size:
752 494 101
BsmI GAATG_Cn'
Cuts at: 0 772 1347
Size: 772 575
Bsp1286I G_dGCh'C
Cuts at: 0 52 59 101 329 595 644 920 1347
Size: 52 7 42 228 266 49 276 427
Fragments arranged by size:
427 276 266 228 52 49 42 7
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 253 308 690 1025 1347
Size: 253 55 382 335 322
Fragments arranged by size:
382 335 322 253 55
BsrDI GCAATG_nn'
Cuts at: 0 588 1347
Size: 588 759
BssSI C'ACGA_G
Cuts at: 0 676 1347
Size: 676 671
BstAPI GCAn_nnn'nTGC
Cuts at: 0 509 1347
Size: 509 838
BstXI CCAn_nnnn'nTGG
Cuts at: 0 652 1347
Size: 652 695
BstYI r'GATC_y
Cuts at: 0 193 266 1347
Size: 193 73 1081
Fragments arranged by size:
1081 193 73
BstZ17I GTA'TAC
Cuts at: 0 780 1347
Size: 780 567
BtgI C'CryG_G
Cuts at: 0 71 399 636 735 1347
Size: 71 328 237 99 612
Fragments arranged by size:
612 328 237 99 71
BtsI GCAGTG_nn'
Cuts at: 0 40 41 1347
Size: 40 1 1306
Fragments arranged by size:
1306 40 1
DraIII CAC_nnn'GTG
Cuts at: 0 164 519 1347
Size: 164 355 828
Fragments arranged by size:
828 355 164
DrdI GACnn_nn'nnGTC
Cuts at: 0 453 1347
Size: 453 894
EaeI y'GGCC_r
Cuts at: 0 185 392 785 1347
Size: 185 207 393 562
Fragments arranged by size:
562 393 207 185
EarI CTCTTCn'nnn_
Cuts at: 0 581 1347
Size: 581 766
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 850 1347
Size: 850 497
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 43 108 283 789 931 1222 1347
Size: 43 65 175 506 142 291 125
Fragments arranged by size:
506 291 175 142 125 65 43
EcoO109I rG'GnC_Cy
Cuts at: 0 278 462 622 665 1279 1347
Size: 278 184 160 43 614 68
Fragments arranged by size:
614 278 184 160 68 43
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 735 909 1347
Size: 735 174 438
Fragments arranged by size:
735 438 174
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 740 1347
Size: 740 607
MscI TGG'CCA
Cuts at: 0 394 787 1347
Size: 394 393 560
Fragments arranged by size:
560 394 393
MslI CAynn'nnrTG
Cuts at: 0 650 676 787 1347
Size: 650 26 111 560
Fragments arranged by size:
650 560 111 26
MspA1I CmG'CkG
Cuts at: 0 170 532 845 1122 1347
Size: 170 362 313 277 225
Fragments arranged by size:
362 313 277 225 170
NcoI C'CATG_G
Cuts at: 0 71 399 1347
Size: 71 328 948
Fragments arranged by size:
948 328 71
NspI r_CATG'y
Cuts at: 0 358 391 1347
Size: 358 33 956
Fragments arranged by size:
956 358 33
PasI CC'CwG_GG
Cuts at: 0 52 1347
Size: 52 1295
PflMI CCAn_nnn'nTGG
Cuts at: 0 72 1347
Size: 72 1275
PfoI T'CCnGG_A
Cuts at: 0 896 1347
Size: 896 451
PpuMI rG'GwC_Cy
Cuts at: 0 462 1279 1347
Size: 462 817 68
Fragments arranged by size:
817 462 68
PspOMI G'GGCC_C
Cuts at: 0 48 1347
Size: 48 1299
PstI C_TGCA'G
Cuts at: 0 250 701 1347
Size: 250 451 646
Fragments arranged by size:
646 451 250
PvuII CAG'CTG
Cuts at: 0 170 1122 1347
Size: 170 952 225
Fragments arranged by size:
952 225 170
SanDI GG'GwC_CC
Cuts at: 0 1279 1347
Size: 1279 68
SexAI A'CCwGG_T
Cuts at: 0 878 1347
Size: 878 469
SfcI C'TryA_G
Cuts at: 0 246 697 1347
Size: 246 451 650
Fragments arranged by size:
650 451 246
SmaI CCC'GGG
Cuts at: 0 872 1347
Size: 872 475
SmlI C'TyrA_G
Cuts at: 0 1035 1347
Size: 1035 312
StuI AGG'CCT
Cuts at: 0 748 1347
Size: 748 599
StyI C'CwwG_G
Cuts at: 0 71 399 1347
Size: 71 328 948
Fragments arranged by size:
948 328 71
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 507 1347
Size: 507 840
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 185 217 990 1022 1347
Size: 185 32 773 32 325
Fragments arranged by size:
773 325 185 32 32
XmaI C'CCGG_G
Cuts at: 0 870 1347
Size: 870 477
Enzymes that do cut:
AarI AccI AleI AlwNI ApaI ApaLI AvaI BamHI
BanI BanII BbsI BciVI BglII Bme1580I BmgBI BmrI
BplI BpmI BpuEI BsaAI BsaHI BsaXI BseRI BseYI
BsgI BsiHKAI BsmI Bsp1286I BspMI BsrDI BssSI BstAPI
BstXI BstYI BstZ17I BtgI BtsI DraIII DrdI EaeI
EarI EciI Eco57MI EcoO109I FalI MmeI MscI MslI
MspA1I NcoI NspI PasI PflMI PfoI PpuMI PspOMI
PstI PvuII SanDI SexAI SfcI SmaI SmlI StuI
StyI TaqII TstI XmaI
Enzymes that do not cut:
AatII Acc65I AclI AcuI AfeI AflII AflIII AgeI
AhdI AlfI AloI ApoI AscI AseI AsiSI AvrII
BaeI BbeI BbvCI BcgI BclI BfrBI BglI BlpI
BmtI Bpu10I BsaI BsaBI BsaWI BsiEI BsiWI BsmBI
BspEI BspHI BsrBI BsrFI BsrGI BssHII BstBI BstEII
Bsu36I BtgZI ClaI CspCI DraI EagI EcoICRI EcoNI
EcoRI EcoRV FseI FspI FspAI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI NaeI NarI
NdeI NgoMIV NheI NotI NruI NsiI PacI PciI
PmeI PmlI PpiI PshAI PsiI PspXI PsrI PvuI
RsrII SacI SacII SalI SapI SbfI ScaI SfiI
SfoI SgfI SgrAI SnaBI SpeI SphI SrfI SspI
SwaI TatI Tth111I XbaI XcmI XhoI XmnI ZraI