Restriction map for
pF1KB7376
(Linear) (Six-base) MAPSORT of: KIBB7376.seq Check: 7992 from: 1 to: 990
KIBB7376.orf
With 182 enzymes: SgfI *
March 3, 2008 11:00 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 98 343 990
Size: 98 245 647
Fragments arranged by size:
647 245 98
AfeI AGC'GCT
Cuts at: 0 200 990
Size: 200 790
AlwNI CAG_nnn'CTG
Cuts at: 0 267 990
Size: 267 723
ApaLI G'TGCA_C
Cuts at: 0 960 990
Size: 960 30
AvaI C'yCGr_G
Cuts at: 0 724 990
Size: 724 266
BbsI GAAGACnn'nnnn_
Cuts at: 0 226 990
Size: 226 764
BclI T'GATC_A
Cuts at: 0 947 990
Size: 947 43
BglI GCCn_nnn'nGGC
Cuts at: 0 193 990
Size: 193 797
Bme1580I G_kGCm'C
Cuts at: 0 964 990
Size: 964 26
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 319 786 990
Size: 319 467 204
Fragments arranged by size:
467 319 204
Bpu10I CC'TnA_GC
Cuts at: 0 889 990
Size: 889 101
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 160 890 990
Size: 160 730 100
Fragments arranged by size:
730 160 100
BsaI GGTCTCn'nnnn_
Cuts at: 0 720 961 990
Size: 720 241 29
Fragments arranged by size:
720 241 29
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 388 583 742 856 990
Size: 388 195 159 114 134
Fragments arranged by size:
388 195 159 134 114
BseYI C'CCAG_C
Cuts at: 0 113 990
Size: 113 877
BsiHKAI G_wGCw'C
Cuts at: 0 964 990
Size: 964 26
BsmBI CGTCTCn'nnnn_
Cuts at: 0 153 534 990
Size: 153 381 456
Fragments arranged by size:
456 381 153
Bsp1286I G_dGCh'C
Cuts at: 0 964 990
Size: 964 26
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 252 990
Size: 252 738
BsrFI r'CCGG_y
Cuts at: 0 648 990
Size: 648 342
BstYI r'GATC_y
Cuts at: 0 23 731 990
Size: 23 708 259
Fragments arranged by size:
708 259 23
Bsu36I CC'TnA_GG
Cuts at: 0 214 990
Size: 214 776
BtgI C'CryG_G
Cuts at: 0 533 612 990
Size: 533 79 378
Fragments arranged by size:
533 378 79
BtsI GCAGTG_nn'
Cuts at: 0 964 990
Size: 964 26
DraI TTT'AAA
Cuts at: 0 882 990
Size: 882 108
EaeI y'GGCC_r
Cuts at: 0 536 990
Size: 536 454
EarI CTCTTCn'nnn_
Cuts at: 0 208 935 990
Size: 208 727 55
Fragments arranged by size:
727 208 55
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 646 990
Size: 646 344
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 98 319 343 786 990
Size: 98 221 24 443 204
Fragments arranged by size:
443 221 204 98 24
EcoNI CCTnn'n_nnAGG
Cuts at: 0 565 589 631 739 990
Size: 565 24 42 108 251
Fragments arranged by size:
565 251 108 42 24
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 45 990
Size: 45 945
HaeII r_GCGC'y
Cuts at: 0 202 520 990
Size: 202 318 470
Fragments arranged by size:
470 318 202
HindIII A'AGCT_T
Cuts at: 0 762 990
Size: 762 228
MspA1I CmG'CkG
Cuts at: 0 117 129 491 535 614 680 704 821
Size: 117 12 362 44 79 66 24 117
Cuts at: 821 990
Size: 169
Fragments arranged by size:
362 169 117 117 79 66 44 24
12
NaeI GCC'GGC
Cuts at: 0 650 990
Size: 650 340
NgoMIV G'CCGG_C
Cuts at: 0 648 990
Size: 648 342
NspI r_CATG'y
Cuts at: 0 361 402 990
Size: 361 41 588
Fragments arranged by size:
588 361 41
PfoI T'CCnGG_A
Cuts at: 0 767 990
Size: 767 223
PshAI GACnn'nnGTC
Cuts at: 0 165 990
Size: 165 825
PsiI TTA'TAA
Cuts at: 0 64 990
Size: 64 926
PstI C_TGCA'G
Cuts at: 0 134 990
Size: 134 856
PvuII CAG'CTG
Cuts at: 0 117 129 704 821 990
Size: 117 12 575 117 169
Fragments arranged by size:
575 169 117 117 12
SacII CC_GC'GG
Cuts at: 0 536 615 990
Size: 536 79 375
Fragments arranged by size:
536 375 79
SapI GCTCTTCn'nnn_
Cuts at: 0 208 990
Size: 208 782
SfcI C'TryA_G
Cuts at: 0 130 990
Size: 130 860
SmlI C'TyrA_G
Cuts at: 0 139 905 990
Size: 139 766 85
Fragments arranged by size:
766 139 85
SphI G_CATG'C
Cuts at: 0 361 990
Size: 361 629
StuI AGG'CCT
Cuts at: 0 595 637 887 990
Size: 595 42 250 103
Fragments arranged by size:
595 250 103 42
StyI C'CwwG_G
Cuts at: 0 442 990
Size: 442 548
TatI w'GTAC_w
Cuts at: 0 227 790 990
Size: 227 563 200
Fragments arranged by size:
563 227 200
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 708 990
Size: 708 282
Enzymes that do cut:
AcuI AfeI AlwNI ApaLI AvaI BbsI BclI BglI
Bme1580I BpmI Bpu10I BpuEI BsaI BseRI BseYI BsiHKAI
BsmBI Bsp1286I BspMI BsrFI BstYI Bsu36I BtgI BtsI
DraI EaeI EarI EciI Eco57MI EcoNI FalI HaeII
HindIII MspA1I NaeI NgoMIV NspI PfoI PshAI PsiI
PstI PvuII SacII SapI SfcI SmlI SphI StuI
StyI TatI XcmI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AflII AflIII AgeI
AhdI AleI AlfI AloI ApaI ApoI AscI AseI
AsiSI AvrII BaeI BamHI BanI BanII BbeI BbvCI
BcgI BciVI BfrBI BglII BlpI BmgBI BmrI BmtI
BplI BsaAI BsaBI BsaHI BsaWI BsaXI BsgI BsiEI
BsiWI BsmI BspEI BspHI BsrBI BsrDI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstZ17I BtgZI ClaI
CspCI DraIII DrdI EagI EcoICRI EcoO109I EcoRI EcoRV
FseI FspI FspAI Hin4I HincII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI NarI NcoI NdeI
NheI NotI NruI NsiI PacI PasI PciI PflMI
PmeI PmlI PpiI PpuMI PspOMI PspXI PsrI PvuI
RsrII SacI SalI SanDI SbfI ScaI SexAI SfiI
SfoI SgfI SgrAI SmaI SnaBI SpeI SrfI SspI
SwaI TaqII TstI Tth111I XbaI XhoI XmaI XmnI
ZraI