(Linear) (Six-base) MAPSORT of: KIBB7375.seq  Check: 1824  from: 1  to: 933



KIBB7375.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:00  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     231     373     933 
   Size:        231     142     560

  Fragments arranged by size: 

                 560     231     142
 
AhdI GACnn_n'nnGTC

Cuts at:      0     595     933 
   Size:        595     338
 
ApoI r'AATT_y

Cuts at:      0     518     933 
   Size:        518     415
 
BanII G_rGCy'C

Cuts at:      0     688     933 
   Size:        688     245
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     417     933 
   Size:        417     516
 
Bme1580I G_kGCm'C

Cuts at:      0     900     933 
   Size:        900      33
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     461     774     933 
   Size:        461     313     159

  Fragments arranged by size: 

                 461     313     159
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     130     175     714     933 
   Size:        130      45     539     219

  Fragments arranged by size: 

                 539     219     130      45
 
BseYI C'CCAG_C

Cuts at:      0      42     933 
   Size:         42     891
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     207     549     933 
   Size:        207     342     384

  Fragments arranged by size: 

                 384     342     207
 
Bsp1286I G_dGCh'C

Cuts at:      0     688     900     933 
   Size:        688     212      33

  Fragments arranged by size: 

                 688     212      33
 
BspHI T'CATG_A

Cuts at:      0     404     428     933 
   Size:        404      24     505

  Fragments arranged by size: 

                 505     404      24
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     274     727     933 
   Size:        274     453     206

  Fragments arranged by size: 

                 453     274     206
 
BsrGI T'GTAC_A

Cuts at:      0     773     933 
   Size:        773     160
 
BtgI C'CryG_G

Cuts at:      0     131     933 
   Size:        131     802
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     453     933 
   Size:        453     480
 
EarI CTCTTCn'nnn_

Cuts at:      0     392     933 
   Size:        392     541
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     231     373     933 
   Size:        231     142     560

  Fragments arranged by size: 

                 560     231     142
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     261     933 
   Size:        261     672
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     581     613     659     691     933 
   Size:        581      32      46      32     242

  Fragments arranged by size: 

                 581     242      46      32      32
 
HincII GTy'rAC

Cuts at:      0     193     933 
   Size:        193     740
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     723     933 
   Size:        723     210
 
MslI CAynn'nnrTG

Cuts at:      0     172     403     679     775     847     933 
   Size:        172     231     276      96      72      86

  Fragments arranged by size: 

                 276     231     172      96      86      72
 
MspA1I CmG'CkG

Cuts at:      0     484     933 
   Size:        484     449
 
NcoI C'CATG_G

Cuts at:      0     131     933 
   Size:        131     802
 
PfoI T'CCnGG_A

Cuts at:      0     442     933 
   Size:        442     491
 
PstI C_TGCA'G

Cuts at:      0     508     933 
   Size:        508     425
 
PvuII CAG'CTG

Cuts at:      0     484     933 
   Size:        484     449
 
SfcI C'TryA_G

Cuts at:      0     504     901     933 
   Size:        504     397      32

  Fragments arranged by size: 

                 504     397      32
 
SspI AAT'ATT

Cuts at:      0     126     933 
   Size:        126     807
 
StuI AGG'CCT

Cuts at:      0     709     933 
   Size:        709     224
 
StyI C'CwwG_G

Cuts at:      0     131     449     933 
   Size:        131     318     484

  Fragments arranged by size: 

                 484     318     131
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0      82     933 
   Size:         82     851
 
TatI w'GTAC_w

Cuts at:      0     176     392     773     933 
   Size:        176     216     381     160

  Fragments arranged by size: 

                 381     216     176     160
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     685     933 
   Size:        685     248
 

 Enzymes that do cut:

     AcuI     AhdI     ApoI    BanII     BbsI Bme1580I     BsaI    BseRI
    BseYI     BsgI Bsp1286I    BspHI    BspMI    BsrGI     BtgI     DrdI
     EarI  Eco57MI     FalI    Hin4I   HincII     MmeI     MslI   MspA1I
     NcoI     PfoI     PstI    PvuII     SfcI     SspI     StuI     StyI
    TaqII     TatI     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     AscI
     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI     BbeI
    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI    BglII     BlpI
    BmgBI     BmrI     BmtI     BplI     BpmI   Bpu10I    BpuEI    BsaAI
    BsaBI    BsaHI    BsaWI    BsaXI    BsiEI  BsiHKAI    BsiWI     BsmI
    BsmBI    BspEI    BsrBI    BsrDI    BsrFI   BssHII    BssSI   BstAPI
    BstBI   BstEII    BstXI    BstYI  BstZ17I   Bsu36I    BtgZI     BtsI
     ClaI    CspCI     DraI   DraIII     EaeI     EagI     EciI  EcoICRI
    EcoNI EcoO109I    EcoRI    EcoRV     FseI     FspI    FspAI    HaeII
  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MscI     NaeI
     NarI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     NspI
     PacI     PasI     PciI    PflMI     PmeI     PmlI     PpiI    PpuMI
    PshAI     PsiI   PspOMI    PspXI     PsrI     PvuI    RsrII     SacI
    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfiI
     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI
     SrfI     SwaI     TstI  Tth111I     XbaI     XhoI     XmaI     XmnI
     ZraI