Restriction map for
pF1KB7369
(Linear) (Six-base) MAPSORT of: KIBB7369.seq Check: 7929 from: 1 to: 954
KIBB7369.orf
With 182 enzymes: SgfI *
March 3, 2008 11:00 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 61 954
Size: 61 893
AleI CACnn'nnGTG
Cuts at: 0 244 954
Size: 244 710
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 38 70 954
Size: 38 32 884
Fragments arranged by size:
884 38 32
ApoI r'AATT_y
Cuts at: 0 31 539 954
Size: 31 508 415
Fragments arranged by size:
508 415 31
AseI AT'TA_AT
Cuts at: 0 658 954
Size: 658 296
BlpI GC'TnA_GC
Cuts at: 0 723 954
Size: 723 231
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 88 661 954
Size: 88 573 293
Fragments arranged by size:
573 293 88
BsaBI GATnn'nnATC
Cuts at: 0 643 954
Size: 643 311
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 678 954
Size: 678 276
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 311 316 954
Size: 311 5 638
Fragments arranged by size:
638 311 5
BsmI GAATG_Cn'
Cuts at: 0 420 954
Size: 420 534
BspHI T'CATG_A
Cuts at: 0 404 551 954
Size: 404 147 403
Fragments arranged by size:
404 403 147
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 727 954
Size: 727 227
BsrDI GCAATG_nn'
Cuts at: 0 588 954
Size: 588 366
BstXI CCAn_nnnn'nTGG
Cuts at: 0 27 954
Size: 27 927
BtgI C'CryG_G
Cuts at: 0 317 428 954
Size: 317 111 526
Fragments arranged by size:
526 317 111
BtsI GCAGTG_nn'
Cuts at: 0 299 628 954
Size: 299 329 326
Fragments arranged by size:
329 326 299
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 617 652 954
Size: 617 35 302
Fragments arranged by size:
617 302 35
EaeI y'GGCC_r
Cuts at: 0 314 602 954
Size: 314 288 352
Fragments arranged by size:
352 314 288
EarI CTCTTCn'nnn_
Cuts at: 0 772 954
Size: 772 182
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 61 954
Size: 61 893
EcoO109I rG'GnC_Cy
Cuts at: 0 440 954
Size: 440 514
EcoRV GAT'ATC
Cuts at: 0 394 954
Size: 394 560
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 725 817 954
Size: 725 92 137
Fragments arranged by size:
725 137 92
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 383 415 954
Size: 383 32 539
Fragments arranged by size:
539 383 32
HincII GTy'rAC
Cuts at: 0 462 954
Size: 462 492
HindIII A'AGCT_T
Cuts at: 0 707 954
Size: 707 247
MfeI C'AATT_G
Cuts at: 0 493 954
Size: 493 461
MscI TGG'CCA
Cuts at: 0 316 604 954
Size: 316 288 350
Fragments arranged by size:
350 316 288
MslI CAynn'nnrTG
Cuts at: 0 25 172 244 403 598 622 775 954
Size: 25 147 72 159 195 24 153 179
Fragments arranged by size:
195 179 159 153 147 72 25 24
NcoI C'CATG_G
Cuts at: 0 317 428 954
Size: 317 111 526
Fragments arranged by size:
526 317 111
PpuMI rG'GwC_Cy
Cuts at: 0 440 954
Size: 440 514
PshAI GACnn'nnGTC
Cuts at: 0 739 954
Size: 739 215
PstI C_TGCA'G
Cuts at: 0 471 508 954
Size: 471 37 446
Fragments arranged by size:
471 446 37
SfcI C'TryA_G
Cuts at: 0 467 504 885 954
Size: 467 37 381 69
Fragments arranged by size:
467 381 69 37
SmlI C'TyrA_G
Cuts at: 0 67 676 954
Size: 67 609 278
Fragments arranged by size:
609 278 67
SpeI A'CTAG_T
Cuts at: 0 243 954
Size: 243 711
SspI AAT'ATT
Cuts at: 0 611 749 954
Size: 611 138 205
Fragments arranged by size:
611 205 138
StyI C'CwwG_G
Cuts at: 0 61 306 317 428 954
Size: 61 245 11 111 526
Fragments arranged by size:
526 245 111 61 11
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 74 954
Size: 74 880
TatI w'GTAC_w
Cuts at: 0 176 471 489 954
Size: 176 295 18 465
Fragments arranged by size:
465 295 176 18
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 216 248 954
Size: 216 32 706
Fragments arranged by size:
706 216 32
XmnI GAAnn'nnTTC
Cuts at: 0 35 954
Size: 35 919
Enzymes that do cut:
AcuI AleI AloI ApoI AseI BlpI BpuEI BsaBI
BseRI BsgI BsmI BspHI BspMI BsrDI BstXI BtgI
BtsI CspCI EaeI EarI Eco57MI EcoO109I EcoRV FalI
Hin4I HincII HindIII MfeI MscI MslI NcoI PpuMI
PshAI PstI SfcI SmlI SpeI SspI StyI TaqII
TatI TstI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AlfI AlwNI ApaI ApaLI AscI AsiSI
AvaI AvrII BaeI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BfrBI BglI BglII Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BsaI BsaAI
BsaHI BsaWI BsaXI BseYI BsiEI BsiHKAI BsiWI BsmBI
Bsp1286I BspEI BsrBI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstYI BstZ17I Bsu36I BtgZI ClaI DraI
DraIII DrdI EagI EciI EcoICRI EcoNI EcoRI FseI
FspI FspAI HaeII HpaI KasI KpnI MluI MmeI
MspA1I NaeI NarI NdeI NgoMIV NheI NotI NruI
NsiI NspI PacI PasI PciI PflMI PfoI PmeI
PmlI PpiI PsiI PspOMI PspXI PsrI PvuI PvuII
RsrII SacI SacII SalI SanDI SapI SbfI ScaI
SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SphI
SrfI StuI SwaI Tth111I XbaI XcmI XhoI XmaI
ZraI