Restriction map for
pF1KB7360
(Linear) (Six-base) MAPSORT of: KIBB7360.seq Check: 5359 from: 1 to: 921
KIBB7360.orf
With 182 enzymes: SgfI *
March 3, 2008 11:00 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 768 921
Size: 768 153
AleI CACnn'nnGTG
Cuts at: 0 577 624 921
Size: 577 47 297
Fragments arranged by size:
577 297 47
AlwNI CAG_nnn'CTG
Cuts at: 0 75 157 287 921
Size: 75 82 130 634
Fragments arranged by size:
634 130 82 75
ApoI r'AATT_y
Cuts at: 0 31 123 921
Size: 31 92 798
Fragments arranged by size:
798 92 31
BanII G_rGCy'C
Cuts at: 0 164 921
Size: 164 757
BbsI GAAGACnn'nnnn_
Cuts at: 0 591 674 921
Size: 591 83 247
Fragments arranged by size:
591 247 83
BfrBI ATG'CAT
Cuts at: 0 211 921
Size: 211 710
BmrI ACTGGGnnnn_n'
Cuts at: 0 48 921
Size: 48 873
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 44 921
Size: 44 877
BsaAI yAC'GTr
Cuts at: 0 716 921
Size: 716 205
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 338 921
Size: 338 583
BseYI C'CCAG_C
Cuts at: 0 795 921
Size: 795 126
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 373 549 921
Size: 373 176 372
Fragments arranged by size:
373 372 176
Bsp1286I G_dGCh'C
Cuts at: 0 164 921
Size: 164 757
BspHI T'CATG_A
Cuts at: 0 404 921
Size: 404 517
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 427 921
Size: 427 494
BsrDI GCAATG_nn'
Cuts at: 0 434 921
Size: 434 487
BsrFI r'CCGG_y
Cuts at: 0 380 921
Size: 380 541
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 354 921
Size: 354 567
DraIII CAC_nnn'GTG
Cuts at: 0 828 921
Size: 828 93
EarI CTCTTCn'nnn_
Cuts at: 0 823 921
Size: 823 98
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 768 921
Size: 768 153
EcoRI G'AATT_C
Cuts at: 0 31 921
Size: 31 890
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 20 42 52 74 241 273 275 307
Size: 20 22 10 22 167 32 2 32
Cuts at: 307 423 455 746 778 921
Size: 116 32 291 32 143
Fragments arranged by size:
291 167 143 116 32 32 32 32
22 22 20 10 2
HindIII A'AGCT_T
Cuts at: 0 884 921
Size: 884 37
MslI CAynn'nnrTG
Cuts at: 0 577 624 921
Size: 577 47 297
Fragments arranged by size:
577 297 47
MspA1I CmG'CkG
Cuts at: 0 519 799 921
Size: 519 280 122
Fragments arranged by size:
519 280 122
NaeI GCC'GGC
Cuts at: 0 382 921
Size: 382 539
NgoMIV G'CCGG_C
Cuts at: 0 380 921
Size: 380 541
NsiI A_TGCA'T
Cuts at: 0 213 921
Size: 213 708
PasI CC'CwG_GG
Cuts at: 0 157 921
Size: 157 764
PfoI T'CCnGG_A
Cuts at: 0 203 921
Size: 203 718
PmlI CAC'GTG
Cuts at: 0 716 921
Size: 716 205
PvuII CAG'CTG
Cuts at: 0 519 799 921
Size: 519 280 122
Fragments arranged by size:
519 280 122
SfcI C'TryA_G
Cuts at: 0 279 921
Size: 279 642
SmlI C'TyrA_G
Cuts at: 0 59 921
Size: 59 862
SspI AAT'ATT
Cuts at: 0 98 921
Size: 98 823
StyI C'CwwG_G
Cuts at: 0 42 318 921
Size: 42 276 603
Fragments arranged by size:
603 276 42
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 739 921
Size: 739 182
Tth111I GACn'n_nGTC
Cuts at: 0 54 921
Size: 54 867
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 474 921
Size: 474 447
Enzymes that do cut:
AcuI AleI AlwNI ApoI BanII BbsI BfrBI BmrI
BpuEI BsaAI BseRI BseYI BsgI Bsp1286I BspHI BspMI
BsrDI BsrFI BtgZI DraIII EarI Eco57MI EcoRI Hin4I
HindIII MslI MspA1I NaeI NgoMIV NsiI PasI PfoI
PmlI PvuII SfcI SmlI SspI StyI TaqII Tth111I
XcmI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AlfI AloI ApaI ApaLI AscI AseI
AsiSI AvaI AvrII BaeI BamHI BanI BbeI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmtI BplI BpmI Bpu10I BsaI BsaBI BsaHI BsaWI
BsaXI BsiEI BsiHKAI BsiWI BsmI BsmBI BspEI BsrBI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BstZ17I Bsu36I BtgI BtsI ClaI CspCI DraI DrdI
EaeI EagI EciI EcoICRI EcoNI EcoO109I EcoRV FalI
FseI FspI FspAI HaeII HincII HpaI KasI KpnI
MfeI MluI MmeI MscI NarI NcoI NdeI NheI
NotI NruI NspI PacI PciI PflMI PmeI PpiI
PpuMI PshAI PsiI PspOMI PspXI PsrI PstI PvuI
RsrII SacI SacII SalI SanDI SapI SbfI ScaI
SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI
SphI SrfI StuI SwaI TatI TstI XbaI XhoI
XmaI XmnI ZraI