Restriction map for
pF1KB7358
(Linear) (Six-base) MAPSORT of: KIBB7358.seq Check: 8996 from: 1 to: 1257
KIBB7358.orf
With 182 enzymes: SgfI *
March 3, 2008 11:00 ..
Acc65I G'GTAC_C
Cuts at: 0 77 1257
Size: 77 1180
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 605 847 881 1257
Size: 605 242 34 376
Fragments arranged by size:
605 376 242 34
BanI G'GyrC_C
Cuts at: 0 77 1257
Size: 77 1180
BbsI GAAGACnn'nnnn_
Cuts at: 0 1027 1257
Size: 1027 230
BciVI GTATCCnnnnn_n'
Cuts at: 0 454 1257
Size: 454 803
BfrBI ATG'CAT
Cuts at: 0 362 1257
Size: 362 895
BglI GCCn_nnn'nGGC
Cuts at: 0 146 1257
Size: 146 1111
BlpI GC'TnA_GC
Cuts at: 0 757 1257
Size: 757 500
BmtI G_CTAG'C
Cuts at: 0 497 1257
Size: 497 760
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 15 123 687 1040 1257
Size: 15 108 564 353 217
Fragments arranged by size:
564 353 217 108 15
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 857 1257
Size: 857 400
BsaBI GATnn'nnATC
Cuts at: 0 175 1141 1257
Size: 175 966 116
Fragments arranged by size:
966 175 116
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 13 43 1257
Size: 13 30 1214
Fragments arranged by size:
1214 30 13
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1095 1257
Size: 1095 162
BseYI C'CCAG_C
Cuts at: 0 426 1065 1093 1257
Size: 426 639 28 164
Fragments arranged by size:
639 426 164 28
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 257 1257
Size: 257 1000
BspHI T'CATG_A
Cuts at: 0 540 1257
Size: 540 717
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 89 1257
Size: 89 1168
BsrDI GCAATG_nn'
Cuts at: 0 761 1257
Size: 761 496
BsrGI T'GTAC_A
Cuts at: 0 1194 1257
Size: 1194 63
BssSI C'ACGA_G
Cuts at: 0 562 1257
Size: 562 695
BstYI r'GATC_y
Cuts at: 0 312 1257
Size: 312 945
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 371 406 1257
Size: 371 35 851
Fragments arranged by size:
851 371 35
EaeI y'GGCC_r
Cuts at: 0 395 476 1257
Size: 395 81 781
Fragments arranged by size:
781 395 81
EarI CTCTTCn'nnn_
Cuts at: 0 581 900 926 1257
Size: 581 319 26 331
Fragments arranged by size:
581 331 319 26
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 15 123 605 687 847 881 1040 1257
Size: 15 108 482 82 160 34 159 217
Fragments arranged by size:
482 217 160 159 108 82 34 15
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 13 45 219 251 580 612 696 728
Size: 13 32 174 32 329 32 84 32
Cuts at: 728 1257
Size: 529
Fragments arranged by size:
529 329 174 84 32 32 32 32
13
KpnI G_GTAC'C
Cuts at: 0 81 1257
Size: 81 1176
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 908 1012 1257
Size: 908 104 245
Fragments arranged by size:
908 245 104
MscI TGG'CCA
Cuts at: 0 397 478 1257
Size: 397 81 779
Fragments arranged by size:
779 397 81
MslI CAynn'nnrTG
Cuts at: 0 43 361 545 748 1257
Size: 43 318 184 203 509
Fragments arranged by size:
509 318 203 184 43
MspA1I CmG'CkG
Cuts at: 0 10 577 1018 1257
Size: 10 567 441 239
Fragments arranged by size:
567 441 239 10
NheI G'CTAG_C
Cuts at: 0 493 1257
Size: 493 764
NsiI A_TGCA'T
Cuts at: 0 364 1257
Size: 364 893
NspI r_CATG'y
Cuts at: 0 362 1257
Size: 362 895
PasI CC'CwG_GG
Cuts at: 0 1217 1257
Size: 1217 40
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 690 722 1257
Size: 690 32 535
Fragments arranged by size:
690 535 32
PstI C_TGCA'G
Cuts at: 0 837 1257
Size: 837 420
PvuII CAG'CTG
Cuts at: 0 10 577 1018 1257
Size: 10 567 441 239
Fragments arranged by size:
567 441 239 10
SfcI C'TryA_G
Cuts at: 0 833 1257
Size: 833 424
SmlI C'TyrA_G
Cuts at: 0 872 1257
Size: 872 385
TatI w'GTAC_w
Cuts at: 0 1194 1257
Size: 1194 63
Tth111I GACn'n_nGTC
Cuts at: 0 1183 1257
Size: 1183 74
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 486 1257
Size: 486 771
Enzymes that do cut:
Acc65I AcuI BanI BbsI BciVI BfrBI BglI BlpI
BmtI BpmI BpuEI BsaBI BsaXI BseRI BseYI BsgI
BspHI BspMI BsrDI BsrGI BssSI BstYI CspCI EaeI
EarI Eco57MI Hin4I KpnI MmeI MscI MslI MspA1I
NheI NsiI NspI PasI PpiI PstI PvuII SfcI
SmlI TatI Tth111I XcmI
Enzymes that do not cut:
AarI AatII AccI AclI AfeI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AscI AseI AsiSI AvaI AvrII BaeI BamHI BanII
BbeI BbvCI BcgI BclI BglII Bme1580I BmgBI BmrI
BplI Bpu10I BsaI BsaAI BsaHI BsaWI BsiEI BsiHKAI
BsiWI BsmI BsmBI Bsp1286I BspEI BsrBI BsrFI BssHII
BstAPI BstBI BstEII BstXI BstZ17I Bsu36I BtgI BtgZI
BtsI ClaI DraI DraIII DrdI EagI EciI EcoICRI
EcoNI EcoO109I EcoRI EcoRV FalI FseI FspI FspAI
HaeII HincII HindIII HpaI KasI MfeI MluI NaeI
NarI NcoI NdeI NgoMIV NotI NruI PacI PciI
PflMI PfoI PmeI PmlI PpuMI PshAI PsiI PspOMI
PspXI PsrI PvuI RsrII SacI SacII SalI SanDI
SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI
SmaI SnaBI SpeI SphI SrfI SspI StuI StyI
SwaI TaqII TstI XbaI XhoI XmaI XmnI ZraI