Restriction map for
pF1KB7354
(Linear) (Six-base) MAPSORT of: KIBB7354.seq Check: 9537 from: 1 to: 3531
KIBB7354.orf
With 182 enzymes: SgfI *
April 24, 2009 13:08 ..
AccI GT'mk_AC
Cuts at: 0 779 925 1302 2804 3531
Size: 779 146 377 1502 727
Fragments arranged by size:
1502 779 727 377 146
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 568 917 959 1319 1667 1742 2889 3531
Size: 568 349 42 360 348 75 1147 642
Fragments arranged by size:
1147 642 568 360 349 348 75 42
AflII C'TTAA_G
Cuts at: 0 2865 3531
Size: 2865 666
AflIII A'CryG_T
Cuts at: 0 555 1208 3244 3531
Size: 555 653 2036 287
Fragments arranged by size:
2036 653 555 287
AlwNI CAG_nnn'CTG
Cuts at: 0 24 301 1481 3198 3531
Size: 24 277 1180 1717 333
Fragments arranged by size:
1717 1180 333 277 24
ApoI r'AATT_y
Cuts at: 0 112 156 171 455 761 1093 1711 1841
Size: 112 44 15 284 306 332 618 130
Cuts at: 1841 1855 1900 3531
Size: 14 45 1631
Fragments arranged by size:
1631 618 332 306 284 130 112 45
44 15 14
AseI AT'TA_AT
Cuts at: 0 1271 1295 3531
Size: 1271 24 2236
Fragments arranged by size:
2236 1271 24
BamHI G'GATC_C
Cuts at: 0 11 2932 3468 3531
Size: 11 2921 536 63
Fragments arranged by size:
2921 536 63 11
BanI G'GyrC_C
Cuts at: 0 1551 1752 3531
Size: 1551 201 1779
Fragments arranged by size:
1779 1551 201
BanII G_rGCy'C
Cuts at: 0 366 3166 3531
Size: 366 2800 365
Fragments arranged by size:
2800 366 365
BbsI GAAGACnn'nnnn_
Cuts at: 0 556 2048 2535 3006 3531
Size: 556 1492 487 471 525
Fragments arranged by size:
1492 556 525 487 471
BclI T'GATC_A
Cuts at: 0 671 3531
Size: 671 2860
BfrBI ATG'CAT
Cuts at: 0 1576 3531
Size: 1576 1955
BmrI ACTGGGnnnn_n'
Cuts at: 0 2126 2670 3531
Size: 2126 544 861
Fragments arranged by size:
2126 861 544
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 3163 3531
Size: 3163 368
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1145 3531
Size: 1145 2386
BsaAI yAC'GTr
Cuts at: 0 69 1209 3531
Size: 69 1140 2322
Fragments arranged by size:
2322 1140 69
BsaBI GATnn'nnATC
Cuts at: 0 1127 1133 1721 3531
Size: 1127 6 588 1810
Fragments arranged by size:
1810 1127 588 6
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 353 383 1722 1752 3531
Size: 353 30 1339 30 1779
Fragments arranged by size:
1779 1339 353 30 30
BseYI C'CCAG_C
Cuts at: 0 24 1118 3531
Size: 24 1094 2413
Fragments arranged by size:
2413 1094 24
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1503 3531
Size: 1503 2028
BsiHKAI G_wGCw'C
Cuts at: 0 366 3531
Size: 366 3165
BsmI GAATG_Cn'
Cuts at: 0 631 3410 3531
Size: 631 2779 121
Fragments arranged by size:
2779 631 121
Bsp1286I G_dGCh'C
Cuts at: 0 366 3166 3531
Size: 366 2800 365
Fragments arranged by size:
2800 366 365
BspHI T'CATG_A
Cuts at: 0 1137 3531
Size: 1137 2394
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 3325 3531
Size: 3325 206
BsrDI GCAATG_nn'
Cuts at: 0 1509 3531
Size: 1509 2022
BsrGI T'GTAC_A
Cuts at: 0 1491 2634 3531
Size: 1491 1143 897
Fragments arranged by size:
1491 1143 897
BstAPI GCAn_nnn'nTGC
Cuts at: 0 3388 3531
Size: 3388 143
BstBI TT'CG_AA
Cuts at: 0 381 438 3531
Size: 381 57 3093
Fragments arranged by size:
3093 381 57
BstXI CCAn_nnnn'nTGG
Cuts at: 0 2986 3531
Size: 2986 545
BstYI r'GATC_y
Cuts at: 0 11 1122 2932 3100 3468 3531
Size: 11 1111 1810 168 368 63
Fragments arranged by size:
1810 1111 368 168 63 11
BstZ17I GTA'TAC
Cuts at: 0 780 926 3531
Size: 780 146 2605
Fragments arranged by size:
2605 780 146
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1541 3531
Size: 1541 1990
BtsI GCAGTG_nn'
Cuts at: 0 2389 3531
Size: 2389 1142
DraI TTT'AAA
Cuts at: 0 1467 3531
Size: 1467 2064
DraIII CAC_nnn'GTG
Cuts at: 0 152 3531
Size: 152 3379
EaeI y'GGCC_r
Cuts at: 0 2160 2976 3151 3531
Size: 2160 816 175 380
Fragments arranged by size:
2160 816 380 175
EarI CTCTTCn'nnn_
Cuts at: 0 186 1181 1338 1465 1967 3531
Size: 186 995 157 127 502 1564
Fragments arranged by size:
1564 995 502 186 157 127
EcoICRI GAG'CTC
Cuts at: 0 364 3531
Size: 364 3167
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 568 917 959 1319 1667 1742 2889 3531
Size: 568 349 42 360 348 75 1147 642
Fragments arranged by size:
1147 642 568 360 349 348 75 42
EcoRI G'AATT_C
Cuts at: 0 1841 3531
Size: 1841 1690
EcoRV GAT'ATC
Cuts at: 0 3261 3531
Size: 3261 270
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 3291 3531
Size: 3291 240
HaeII r_GCGC'y
Cuts at: 0 9 3531
Size: 9 3522
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 656 688 861 893 2769 2801 3069 3101
Size: 656 32 173 32 1876 32 268 32
Cuts at: 3101 3531
Size: 430
Fragments arranged by size:
1876 656 430 268 173 32 32 32
32
MfeI C'AATT_G
Cuts at: 0 2230 3531
Size: 2230 1301
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1544 1568 3378 3460 3531
Size: 1544 24 1810 82 71
Fragments arranged by size:
1810 1544 82 71 24
MscI TGG'CCA
Cuts at: 0 2162 2978 3153 3531
Size: 2162 816 175 378
Fragments arranged by size:
2162 816 378 175
MslI CAynn'nnrTG
Cuts at: 0 47 421 787 1850 3022 3531
Size: 47 374 366 1063 1172 509
Fragments arranged by size:
1172 1063 509 374 366 47
MspA1I CmG'CkG
Cuts at: 0 24 1968 1987 2137 2365 3531
Size: 24 1944 19 150 228 1166
Fragments arranged by size:
1944 1166 228 150 24 19
NsiI A_TGCA'T
Cuts at: 0 1578 3531
Size: 1578 1953
NspI r_CATG'y
Cuts at: 0 559 2803 3248 3531
Size: 559 2244 445 283
Fragments arranged by size:
2244 559 445 283
PciI A'CATG_T
Cuts at: 0 555 3244 3531
Size: 555 2689 287
Fragments arranged by size:
2689 555 287
PflMI CCAn_nnn'nTGG
Cuts at: 0 687 3531
Size: 687 2844
PfoI T'CCnGG_A
Cuts at: 0 3368 3531
Size: 3368 163
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1442 1474 3531
Size: 1442 32 2057
Fragments arranged by size:
2057 1442 32
PsiI TTA'TAA
Cuts at: 0 2197 2651 3531
Size: 2197 454 880
Fragments arranged by size:
2197 880 454
PstI C_TGCA'G
Cuts at: 0 468 843 2396 3531
Size: 468 375 1553 1135
Fragments arranged by size:
1553 1135 468 375
PvuII CAG'CTG
Cuts at: 0 24 1987 2137 2365 3531
Size: 24 1963 150 228 1166
Fragments arranged by size:
1963 1166 228 150 24
SacI G_AGCT'C
Cuts at: 0 366 3531
Size: 366 3165
SapI GCTCTTCn'nnn_
Cuts at: 0 1181 3531
Size: 1181 2350
ScaI AGT'ACT
Cuts at: 0 1570 3303 3531
Size: 1570 1733 228
Fragments arranged by size:
1733 1570 228
SfcI C'TryA_G
Cuts at: 0 464 839 2392 3531
Size: 464 375 1553 1139
Fragments arranged by size:
1553 1139 464 375
SmlI C'TyrA_G
Cuts at: 0 1160 2865 3531
Size: 1160 1705 666
Fragments arranged by size:
1705 1160 666
SnaBI TAC'GTA
Cuts at: 0 69 3531
Size: 69 3462
SspI AAT'ATT
Cuts at: 0 2751 3531
Size: 2751 780
StyI C'CwwG_G
Cuts at: 0 958 3531
Size: 958 2573
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 415 3296 3531
Size: 415 2881 235
Fragments arranged by size:
2881 415 235
TatI w'GTAC_w
Cuts at: 0 143 1491 1568 2634 3301 3531
Size: 143 1348 77 1066 667 230
Fragments arranged by size:
1348 1066 667 230 143 77
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1722 1754 3343 3375 3531
Size: 1722 32 1589 32 156
Fragments arranged by size:
1722 1589 156 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1294 3231 3531
Size: 1294 1937 300
Fragments arranged by size:
1937 1294 300
XmnI GAAnn'nnTTC
Cuts at: 0 458 2358 3531
Size: 458 1900 1173
Fragments arranged by size:
1900 1173 458
Enzymes that do cut:
AccI AcuI AflII AflIII AlwNI ApoI AseI BamHI
BanI BanII BbsI BclI BfrBI BmrI BplI BpuEI
BsaAI BsaBI BsaXI BseYI BsgI BsiHKAI BsmI Bsp1286I
BspHI BspMI BsrDI BsrGI BstAPI BstBI BstXI BstYI
BstZ17I BtgZI BtsI DraI DraIII EaeI EarI EcoICRI
Eco57MI EcoRI EcoRV FalI HaeII Hin4I MfeI MmeI
MscI MslI MspA1I NsiI NspI PciI PflMI PfoI
PpiI PsiI PstI PvuII SacI SapI ScaI SfcI
SmlI SnaBI SspI StyI TaqII TatI TstI XcmI
XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AgeI AhdI AleI
AlfI AloI ApaI ApaLI AscI AsiSI AvaI AvrII
BaeI BbeI BbvCI BcgI BciVI BglI BglII BlpI
Bme1580I BmgBI BmtI BpmI Bpu10I BsaI BsaHI BsaWI
BseRI BsiEI BsiWI BsmBI BspEI BsrBI BsrFI BssHII
BssSI BstEII Bsu36I BtgI ClaI CspCI DrdI EagI
EciI EcoNI EcoO109I FseI FspI FspAI HincII HindIII
HpaI KasI KpnI MluI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI PacI PasI PmeI PmlI
PpuMI PshAI PspOMI PspXI PsrI PvuI RsrII SacII
SalI SanDI SbfI SexAI SfiI SfoI SgfI SgrAI
SmaI SpeI SphI SrfI StuI SwaI Tth111I XbaI
XhoI XmaI ZraI