Restriction map for
pF1KB7349
(Linear) (Six-base) MAPSORT of: KIBB7349.seq Check: 4410 from: 1 to: 3048
KIBB7349.orf
With 182 enzymes: SgfI *
April 24, 2009 13:08 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 312 839 2571 2583 3048
Size: 312 527 1732 12 465
Fragments arranged by size:
1732 527 465 312 12
AatII G_ACGT'C
Cuts at: 0 20 3048
Size: 20 3028
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 457 556 643 655 1596 1683 1736 1891
Size: 457 99 87 12 941 87 53 155
Cuts at: 1891 2269 2284 2401 2674 3048
Size: 378 15 117 273 374
Fragments arranged by size:
941 457 378 374 273 155 117 99
87 87 53 15 12
AfeI AGC'GCT
Cuts at: 0 1093 2050 2484 3048
Size: 1093 957 434 564
Fragments arranged by size:
1093 957 564 434
AflIII A'CryG_T
Cuts at: 0 35 3048
Size: 35 3013
AleI CACnn'nnGTG
Cuts at: 0 2960 3048
Size: 2960 88
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2086 2327 2651 2872 3048
Size: 2086 241 324 221 176
Fragments arranged by size:
2086 324 241 221 176
AlwNI CAG_nnn'CTG
Cuts at: 0 54 66 327 1455 1923 3000 3048
Size: 54 12 261 1128 468 1077 48
Fragments arranged by size:
1128 1077 468 261 54 48 12
ApaI G_GGCC'C
Cuts at: 0 1396 2417 3048
Size: 1396 1021 631
Fragments arranged by size:
1396 1021 631
ApaLI G'TGCA_C
Cuts at: 0 628 3048
Size: 628 2420
ApoI r'AATT_y
Cuts at: 0 1951 3048
Size: 1951 1097
AvaI C'yCGr_G
Cuts at: 0 235 883 1489 2095 2843 3048
Size: 235 648 606 606 748 205
Fragments arranged by size:
748 648 606 606 235 205
BamHI G'GATC_C
Cuts at: 0 1640 2901 3048
Size: 1640 1261 147
Fragments arranged by size:
1640 1261 147
BanI G'GyrC_C
Cuts at: 0 2962 3048
Size: 2962 86
BanII G_rGCy'C
Cuts at: 0 236 884 1034 1396 1546 1556 1637 2354
Size: 236 648 150 362 150 10 81 717
Cuts at: 2354 2417 2890 2936 3048
Size: 63 473 46 112
Fragments arranged by size:
717 648 473 362 236 150 150 112
81 63 46 10
BbsI GAAGACnn'nnnn_
Cuts at: 0 412 1724 2389 3048
Size: 412 1312 665 659
Fragments arranged by size:
1312 665 659 412
BbvCI CC'TCA_GC
Cuts at: 0 1769 3048
Size: 1769 1279
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1186 1220 3048
Size: 1186 34 1828
Fragments arranged by size:
1828 1186 34
BglI GCCn_nnn'nGGC
Cuts at: 0 1231 1790 3048
Size: 1231 559 1258
Fragments arranged by size:
1258 1231 559
BlpI GC'TnA_GC
Cuts at: 0 317 664 979 1033 2153 3048
Size: 317 347 315 54 1120 895
Fragments arranged by size:
1120 895 347 317 315 54
Bme1580I G_kGCm'C
Cuts at: 0 632 1396 2417 2967 3048
Size: 632 764 1021 550 81
Fragments arranged by size:
1021 764 632 550 81
BmgBI CAC'GTC
Cuts at: 0 36 948 3048
Size: 36 912 2100
Fragments arranged by size:
2100 912 36
BmrI ACTGGGnnnn_n'
Cuts at: 0 1158 3048
Size: 1158 1890
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1539 1824 3048
Size: 1539 285 1224
Fragments arranged by size:
1539 1224 285
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 25 202 286 361 706 1072 1153 1915
Size: 25 177 84 75 345 366 81 762
Cuts at: 1915 1963 1977 2550 2689 3048
Size: 48 14 573 139 359
Fragments arranged by size:
762 573 366 359 345 177 139 84
81 75 48 25 14
Bpu10I CC'TnA_GC
Cuts at: 0 1435 1769 2032 2617 3048
Size: 1435 334 263 585 431
Fragments arranged by size:
1435 585 431 334 263
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2029 2338 3048
Size: 2029 309 710
Fragments arranged by size:
2029 710 309
BsaI GGTCTCn'nnnn_
Cuts at: 0 204 607 3048
Size: 204 403 2441
Fragments arranged by size:
2441 403 204
BsaHI Gr'CG_yC
Cuts at: 0 17 1142 3048
Size: 17 1125 1906
Fragments arranged by size:
1906 1125 17
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 22 52 1090 1120 3048
Size: 22 30 1038 30 1928
Fragments arranged by size:
1928 1038 30 30 22
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 43 283 286 478 541 598 661 861
Size: 43 240 3 192 63 57 63 200
Cuts at: 861 862 1012 1066 1849 2155 2464 3001 3048
Size: 1 150 54 783 306 309 537 47
Fragments arranged by size:
783 537 309 306 240 200 192 150
63 63 57 54 47 43 3 1
BseYI C'CCAG_C
Cuts at: 0 783 1687 1991 2074 3029 3048
Size: 783 904 304 83 955 19
Fragments arranged by size:
955 904 783 304 83 19
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 436 449 2002 2160 3048
Size: 436 13 1553 158 888
Fragments arranged by size:
1553 888 436 158 13
BsiEI CG_ry'CG
Cuts at: 0 349 1010 3048
Size: 349 661 2038
Fragments arranged by size:
2038 661 349
BsiHKAI G_wGCw'C
Cuts at: 0 236 632 884 938 980 1034 1076 1498
Size: 236 396 252 54 42 54 42 422
Cuts at: 1498 2354 2890 3048
Size: 856 536 158
Fragments arranged by size:
856 536 422 396 252 236 158 54
54 42 42
BsmI GAATG_Cn'
Cuts at: 0 357 1464 2157 3048
Size: 357 1107 693 891
Fragments arranged by size:
1107 891 693 357
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1129 3048
Size: 1129 1919
Bsp1286I G_dGCh'C
Cuts at: 0 236 632 884 938 980 1034 1076 1396
Size: 236 396 252 54 42 54 42 320
Cuts at: 1396 1498 1546 1556 1637 2354 2417 2890 2936
Size: 102 48 10 81 717 63 473 46
Cuts at: 2936 2967 3048
Size: 31 81
Fragments arranged by size:
717 473 396 320 252 236 102 81
81 63 54 54 48 46 42 42
31 10
BspHI T'CATG_A
Cuts at: 0 2024 3048
Size: 2024 1024
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 94 312 668 839 1628 2571 2583 3048
Size: 94 218 356 171 789 943 12 465
Fragments arranged by size:
943 789 465 356 218 171 94 12
BsrBI CCG'CTC
Cuts at: 0 383 545 929 1818 2343 2406 2559 3048
Size: 383 162 384 889 525 63 153 489
Fragments arranged by size:
889 525 489 384 383 162 153 63
BsrFI r'CCGG_y
Cuts at: 0 104 148 3048
Size: 104 44 2900
Fragments arranged by size:
2900 104 44
BssHII G'CGCG_C
Cuts at: 0 569 3048
Size: 569 2479
BssSI C'ACGA_G
Cuts at: 0 937 1195 3048
Size: 937 258 1853
Fragments arranged by size:
1853 937 258
BstAPI GCAn_nnn'nTGC
Cuts at: 0 54 86 327 1761 3048
Size: 54 32 241 1434 1287
Fragments arranged by size:
1434 1287 241 54 32
BstYI r'GATC_y
Cuts at: 0 1640 2711 2901 3048
Size: 1640 1071 190 147
Fragments arranged by size:
1640 1071 190 147
Bsu36I CC'TnA_GG
Cuts at: 0 758 1897 3048
Size: 758 1139 1151
Fragments arranged by size:
1151 1139 758
BtgI C'CryG_G
Cuts at: 0 174 2283 3048
Size: 174 2109 765
Fragments arranged by size:
2109 765 174
BtsI GCAGTG_nn'
Cuts at: 0 1528 2709 3048
Size: 1528 1181 339
Fragments arranged by size:
1528 1181 339
ClaI AT'CG_AT
Cuts at: 0 1286 3048
Size: 1286 1762
DraI TTT'AAA
Cuts at: 0 2762 3048
Size: 2762 286
DraIII CAC_nnn'GTG
Cuts at: 0 2704 3048
Size: 2704 344
DrdI GACnn_nn'nnGTC
Cuts at: 0 1197 2647 3048
Size: 1197 1450 401
Fragments arranged by size:
1450 1197 401
EaeI y'GGCC_r
Cuts at: 0 150 590 917 1007 2228 3048
Size: 150 440 327 90 1221 820
Fragments arranged by size:
1221 820 440 327 150 90
EagI C'GGCC_G
Cuts at: 0 1007 3048
Size: 1007 2041
EarI CTCTTCn'nnn_
Cuts at: 0 275 839 2026 2132 3048
Size: 275 564 1187 106 916
Fragments arranged by size:
1187 916 564 275 106
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 806 2849 3048
Size: 806 2043 199
Fragments arranged by size:
2043 806 199
EcoICRI GAG'CTC
Cuts at: 0 234 882 1032 2352 2888 3048
Size: 234 648 150 1320 536 160
Fragments arranged by size:
1320 648 536 234 160 150
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 25 202 286 361 457 556 643 655
Size: 25 177 84 75 96 99 87 12
Cuts at: 655 706 1072 1153 1596 1683 1736 1891 1915
Size: 51 366 81 443 87 53 155 24
Cuts at: 1915 1963 1977 2269 2284 2401 2550 2674 2689
Size: 48 14 292 15 117 149 124 15
Cuts at: 2689 3048
Size: 359
Fragments arranged by size:
443 366 359 292 177 155 149 124
117 99 96 87 87 84 81 75
53 51 48 25 24 15 15 14
12
EcoNI CCTnn'n_nnAGG
Cuts at: 0 391 3048
Size: 391 2657
EcoO109I rG'GnC_Cy
Cuts at: 0 558 754 1374 1392 1393 1617 2413 2649
Size: 558 196 620 18 1 224 796 236
Cuts at: 2649 2979 3048
Size: 330 69
Fragments arranged by size:
796 620 558 330 236 224 196 69
18 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 2025 2761 3021 3048
Size: 2025 736 260 27
Fragments arranged by size:
2025 736 260 27
FspI TGC'GCA
Cuts at: 0 112 3048
Size: 112 2936
HaeII r_GCGC'y
Cuts at: 0 126 870 955 1095 1894 2052 2486 3048
Size: 126 744 85 140 799 158 434 562
Fragments arranged by size:
799 744 562 434 158 140 126 85
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1090 1122 1573 1605 1657 1689 2136 2168
Size: 1090 32 451 32 52 32 447 32
Cuts at: 2168 3048
Size: 880
Fragments arranged by size:
1090 880 451 447 52 32 32 32
32
HindIII A'AGCT_T
Cuts at: 0 496 3048
Size: 496 2552
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 938 1106 1236 3048
Size: 938 168 130 1812
Fragments arranged by size:
1812 938 168 130
MscI TGG'CCA
Cuts at: 0 919 3048
Size: 919 2129
MslI CAynn'nnrTG
Cuts at: 0 214 740 1426 1978 2239 2960 3048
Size: 214 526 686 552 261 721 88
Fragments arranged by size:
721 686 552 526 261 214 88
MspA1I CmG'CkG
Cuts at: 0 45 66 294 1037 1758 1869 1935 2189
Size: 45 21 228 743 721 111 66 254
Cuts at: 2189 2439 2508 2550 2625 2687 3048
Size: 250 69 42 75 62 361
Fragments arranged by size:
743 721 361 254 250 228 111 75
69 66 62 45 42 21
NotI GC'GGCC_GC
Cuts at: 0 1007 3048
Size: 1007 2041
NspI r_CATG'y
Cuts at: 0 1977 3048
Size: 1977 1071
PasI CC'CwG_GG
Cuts at: 0 387 1182 1397 1548 2645 2986 3048
Size: 387 795 215 151 1097 341 62
Fragments arranged by size:
1097 795 387 341 215 151 62
PflMI CCAn_nnn'nTGG
Cuts at: 0 1860 3048
Size: 1860 1188
PfoI T'CCnGG_A
Cuts at: 0 2270 3048
Size: 2270 778
PpuMI rG'GwC_Cy
Cuts at: 0 558 754 1617 2649 2979 3048
Size: 558 196 863 1032 330 69
Fragments arranged by size:
1032 863 558 330 196 69
PspOMI G'GGCC_C
Cuts at: 0 1392 2413 3048
Size: 1392 1021 635
Fragments arranged by size:
1392 1021 635
PspXI vC'TCGA_Gb
Cuts at: 0 235 883 2095 3048
Size: 235 648 1212 953
Fragments arranged by size:
1212 953 648 235
PstI C_TGCA'G
Cuts at: 0 50 101 332 836 1022 1058 1222 1448
Size: 50 51 231 504 186 36 164 226
Cuts at: 1448 1757 1919 2513 2549 2567 2609 2630 3048
Size: 309 162 594 36 18 42 21 418
Fragments arranged by size:
594 504 418 309 231 226 186 164
162 51 50 42 36 36 21 18
PvuII CAG'CTG
Cuts at: 0 45 66 1758 1869 2189 2508 2550 2625
Size: 45 21 1692 111 320 319 42 75
Cuts at: 2625 2687 3048
Size: 62 361
Fragments arranged by size:
1692 361 320 319 111 75 62 45
42 21
RsrII CG'GwC_CG
Cuts at: 0 1209 3048
Size: 1209 1839
SacI G_AGCT'C
Cuts at: 0 236 884 1034 2354 2890 3048
Size: 236 648 150 1320 536 158
Fragments arranged by size:
1320 648 536 236 158 150
SapI GCTCTTCn'nnn_
Cuts at: 0 275 2026 3048
Size: 275 1751 1022
Fragments arranged by size:
1751 1022 275
SfcI C'TryA_G
Cuts at: 0 46 97 328 832 1018 1054 1218 1444
Size: 46 51 231 504 186 36 164 226
Cuts at: 1444 1753 1915 2434 2478 2509 2545 2563 2605
Size: 309 162 519 44 31 36 18 42
Cuts at: 2605 2626 3048
Size: 21 422
Fragments arranged by size:
519 504 422 309 231 226 186 164
162 51 46 44 42 36 36 31
21 18
SmaI CCC'GGG
Cuts at: 0 2845 3048
Size: 2845 203
SmlI C'TyrA_G
Cuts at: 0 235 883 2044 2095 2353 3048
Size: 235 648 1161 51 258 695
Fragments arranged by size:
1161 695 648 258 235 51
StuI AGG'CCT
Cuts at: 0 704 1704 2173 3048
Size: 704 1000 469 875
Fragments arranged by size:
1000 875 704 469
StyI C'CwwG_G
Cuts at: 0 503 1538 1584 2315 2321 2918 3048
Size: 503 1035 46 731 6 597 130
Fragments arranged by size:
1035 731 597 503 130 46 6
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 363 631 775 2950 3048
Size: 363 268 144 2175 98
Fragments arranged by size:
2175 363 268 144 98
TatI w'GTAC_w
Cuts at: 0 2120 2213 3048
Size: 2120 93 835
Fragments arranged by size:
2120 835 93
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1333 1365 3048
Size: 1333 32 1683
Fragments arranged by size:
1683 1333 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1290 3048
Size: 1290 1758
XhoI C'TCGA_G
Cuts at: 0 235 883 2095 3048
Size: 235 648 1212 953
Fragments arranged by size:
1212 953 648 235
XmaI C'CCGG_G
Cuts at: 0 2843 3048
Size: 2843 205
XmnI GAAnn'nnTTC
Cuts at: 0 284 1462 3048
Size: 284 1178 1586
Fragments arranged by size:
1586 1178 284
ZraI GAC'GTC
Cuts at: 0 18 3048
Size: 18 3030
Enzymes that do cut:
AarI AatII AcuI AfeI AflIII AleI AlfI AlwNI
ApaI ApaLI ApoI AvaI BamHI BanI BanII BbsI
BbvCI BcgI BglI BlpI Bme1580I BmgBI BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaHI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspHI BspMI
BsrBI BsrFI BssHII BssSI BstAPI BstYI Bsu36I BtgI
BtsI ClaI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I FalI FspI HaeII
Hin4I HindIII MmeI MscI MslI MspA1I NotI NspI
PasI PflMI PfoI PpuMI PspOMI PspXI PstI PvuII
RsrII SacI SapI SfcI SmaI SmlI StuI StyI
TaqII TatI TstI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AccI Acc65I AclI AflII AgeI AhdI AloI AscI
AseI AsiSI AvrII BaeI BbeI BciVI BclI BfrBI
BglII BmtI BsaAI BsaBI BsaWI BsiWI BspEI BsrDI
BsrGI BstBI BstEII BstXI BstZ17I BtgZI CspCI EcoRI
EcoRV FseI FspAI HincII HpaI KasI KpnI MfeI
MluI NaeI NarI NcoI NdeI NgoMIV NheI NruI
NsiI PacI PciI PmeI PmlI PpiI PshAI PsiI
PsrI PvuI SacII SalI SanDI SbfI ScaI SexAI
SfiI SfoI SgfI SgrAI SnaBI SpeI SphI SrfI
SspI SwaI Tth111I XbaI