Restriction map for
pF1KB7335
(Linear) (Six-base) MAPSORT of: KIBB7335.seq Check: 5890 from: 1 to: 3060
KIBB7335.orf
With 182 enzymes: SgfI *
April 24, 2009 13:08 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 33 2959 3060
Size: 33 2926 101
Fragments arranged by size:
2926 101 33
AccI GT'mk_AC
Cuts at: 0 1019 3060
Size: 1019 2041
Acc65I G'GTAC_C
Cuts at: 0 243 3060
Size: 243 2817
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 139 1513 2489 2504 2597 3060
Size: 139 1374 976 15 93 463
Fragments arranged by size:
1374 976 463 139 93 15
AfeI AGC'GCT
Cuts at: 0 2389 3060
Size: 2389 671
AleI CACnn'nnGTG
Cuts at: 0 1624 2591 2719 3060
Size: 1624 967 128 341
Fragments arranged by size:
1624 967 341 128
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 206 238 3060
Size: 206 32 2822
Fragments arranged by size:
2822 206 32
AlwNI CAG_nnn'CTG
Cuts at: 0 501 1549 2196 2787 3060
Size: 501 1048 647 591 273
Fragments arranged by size:
1048 647 591 501 273
ApaI G_GGCC'C
Cuts at: 0 1360 3060
Size: 1360 1700
ApaLI G'TGCA_C
Cuts at: 0 1424 3060
Size: 1424 1636
ApoI r'AATT_y
Cuts at: 0 1710 3060
Size: 1710 1350
AseI AT'TA_AT
Cuts at: 0 934 3060
Size: 934 2126
AvaI C'yCGr_G
Cuts at: 0 254 1361 1794 3060
Size: 254 1107 433 1266
Fragments arranged by size:
1266 1107 433 254
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 788 821 2860 2893 3060
Size: 788 33 2039 33 167
Fragments arranged by size:
2039 788 167 33 33
BanI G'GyrC_C
Cuts at: 0 243 1182 1332 2283 3060
Size: 243 939 150 951 777
Fragments arranged by size:
951 939 777 243 150
BanII G_rGCy'C
Cuts at: 0 1360 2705 3060
Size: 1360 1345 355
Fragments arranged by size:
1360 1345 355
BbsI GAAGACnn'nnnn_
Cuts at: 0 641 3060
Size: 641 2419
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1423 1457 1720 1754 3060
Size: 1423 34 263 34 1306
Fragments arranged by size:
1423 1306 263 34 34
BclI T'GATC_A
Cuts at: 0 782 3060
Size: 782 2278
BfrBI ATG'CAT
Cuts at: 0 1737 1982 3060
Size: 1737 245 1078
Fragments arranged by size:
1737 1078 245
BglII A'GATC_T
Cuts at: 0 344 443 3060
Size: 344 99 2617
Fragments arranged by size:
2617 344 99
BlpI GC'TnA_GC
Cuts at: 0 2340 2851 3060
Size: 2340 511 209
Fragments arranged by size:
2340 511 209
Bme1580I G_kGCm'C
Cuts at: 0 923 1360 1428 1926 2286 3060
Size: 923 437 68 498 360 774
Fragments arranged by size:
923 774 498 437 360 68
BmrI ACTGGGnnnn_n'
Cuts at: 0 673 1637 1662 3060
Size: 673 964 25 1398
Fragments arranged by size:
1398 964 673 25
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1609 2712 3060
Size: 1609 1103 348
Fragments arranged by size:
1609 1103 348
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1366 1592 1621 1777 1890 1902 2554 2707
Size: 1366 226 29 156 113 12 652 153
Cuts at: 2707 2764 2998 3060
Size: 57 234 62
Fragments arranged by size:
1366 652 234 226 156 153 113 62
57 29 12
Bpu10I CC'TnA_GC
Cuts at: 0 304 384 986 1550 3060
Size: 304 80 602 564 1510
Fragments arranged by size:
1510 602 564 304 80
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2070 3060
Size: 2070 990
BsaI GGTCTCn'nnnn_
Cuts at: 0 1128 2925 3060
Size: 1128 1797 135
Fragments arranged by size:
1797 1128 135
BsaHI Gr'CG_yC
Cuts at: 0 680 2359 3060
Size: 680 1679 701
Fragments arranged by size:
1679 701 680
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 334 364 2614 2644 2722 2752 3060
Size: 334 30 2250 30 78 30 308
Fragments arranged by size:
2250 334 308 78 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 459 899 1027 1530 1833 2443 2957 3060
Size: 459 440 128 503 303 610 514 103
Fragments arranged by size:
610 514 503 459 440 303 128 103
BseYI C'CCAG_C
Cuts at: 0 37 530 2178 2447 3060
Size: 37 493 1648 269 613
Fragments arranged by size:
1648 613 493 269 37
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 100 148 788 1714 1848 3060
Size: 100 48 640 926 134 1212
Fragments arranged by size:
1212 926 640 134 100 48
BsiHKAI G_wGCw'C
Cuts at: 0 1118 1428 1631 2705 3060
Size: 1118 310 203 1074 355
Fragments arranged by size:
1118 1074 355 310 203
BsiWI C'GTAC_G
Cuts at: 0 1212 3060
Size: 1212 1848
BsmBI CGTCTCn'nnnn_
Cuts at: 0 687 3060
Size: 687 2373
Bsp1286I G_dGCh'C
Cuts at: 0 923 1118 1360 1428 1631 1926 2286 2705
Size: 923 195 242 68 203 295 360 419
Cuts at: 2705 3060
Size: 355
Fragments arranged by size:
923 419 360 355 295 242 203 195
68
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 33 2959 3060
Size: 33 2926 101
Fragments arranged by size:
2926 101 33
BsrBI CCG'CTC
Cuts at: 0 1583 2339 3060
Size: 1583 756 721
Fragments arranged by size:
1583 756 721
BsrDI GCAATG_nn'
Cuts at: 0 956 1809 2441 3060
Size: 956 853 632 619
Fragments arranged by size:
956 853 632 619
BsrFI r'CCGG_y
Cuts at: 0 2022 2204 3060
Size: 2022 182 856
Fragments arranged by size:
2022 856 182
BstEII G'GTnAC_C
Cuts at: 0 258 795 3060
Size: 258 537 2265
Fragments arranged by size:
2265 537 258
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1645 3060
Size: 1645 1415
BstYI r'GATC_y
Cuts at: 0 344 418 443 1832 2060 2801 2963 3060
Size: 344 74 25 1389 228 741 162 97
Fragments arranged by size:
1389 741 344 228 162 97 74 25
Bsu36I CC'TnA_GG
Cuts at: 0 1352 3060
Size: 1352 1708
BtgI C'CryG_G
Cuts at: 0 686 1700 2821 3060
Size: 686 1014 1121 239
Fragments arranged by size:
1121 1014 686 239
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 930 1723 1737 3060
Size: 930 793 14 1323
Fragments arranged by size:
1323 930 793 14
BtsI GCAGTG_nn'
Cuts at: 0 739 829 855 1062 2317 2511 3060
Size: 739 90 26 207 1255 194 549
Fragments arranged by size:
1255 739 549 207 194 90 26
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 2306 2341 3060
Size: 2306 35 719
Fragments arranged by size:
2306 719 35
DraIII CAC_nnn'GTG
Cuts at: 0 498 3060
Size: 498 2562
DrdI GACnn_nn'nnGTC
Cuts at: 0 2953 3060
Size: 2953 107
EaeI y'GGCC_r
Cuts at: 0 590 912 1253 3060
Size: 590 322 341 1807
Fragments arranged by size:
1807 590 341 322
EarI CTCTTCn'nnn_
Cuts at: 0 1600 2051 2352 2573 2617 3060
Size: 1600 451 301 221 44 443
Fragments arranged by size:
1600 451 443 301 221 44
EcoICRI GAG'CTC
Cuts at: 0 2703 3060
Size: 2703 357
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 139 1366 1513 1592 1621 1777 1890 1902
Size: 139 1227 147 79 29 156 113 12
Cuts at: 1902 2489 2504 2554 2597 2707 2764 2998 3060
Size: 587 15 50 43 110 57 234 62
Fragments arranged by size:
1227 587 234 156 147 139 113 110
79 62 57 50 43 29 15 12
EcoNI CCTnn'n_nnAGG
Cuts at: 0 577 3051 3060
Size: 577 2474 9
Fragments arranged by size:
2474 577 9
EcoO109I rG'GnC_Cy
Cuts at: 0 657 965 1265 1356 1357 1974 2193 2682
Size: 657 308 300 91 1 617 219 489
Cuts at: 2682 2752 2784 2896 3060
Size: 70 32 112 164
Fragments arranged by size:
657 617 489 308 300 219 164 112
91 70 32 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 2286 2494 3060
Size: 2286 208 566
Fragments arranged by size:
2286 566 208
FspI TGC'GCA
Cuts at: 0 1054 1312 3060
Size: 1054 258 1748
Fragments arranged by size:
1748 1054 258
FspAI rTGC'GCAy
Cuts at: 0 1312 3060
Size: 1312 1748
HaeII r_GCGC'y
Cuts at: 0 15 1812 2391 3060
Size: 15 1797 579 669
Fragments arranged by size:
1797 669 579 15
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 410 442 684 716 2632 2664 3060
Size: 410 32 242 32 1916 32 396
Fragments arranged by size:
1916 410 396 242 32 32 32
KpnI G_GTAC'C
Cuts at: 0 247 3060
Size: 247 2813
MfeI C'AATT_G
Cuts at: 0 1270 3060
Size: 1270 1790
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 278 2499 3060
Size: 278 2221 561
Fragments arranged by size:
2221 561 278
MscI TGG'CCA
Cuts at: 0 592 914 1255 3060
Size: 592 322 341 1805
Fragments arranged by size:
1805 592 341 322
MslI CAynn'nnrTG
Cuts at: 0 949 1624 2524 2591 2719 3060
Size: 949 675 900 67 128 341
Fragments arranged by size:
949 900 675 341 128 67
MspA1I CmG'CkG
Cuts at: 0 380 534 697 2575 2874 3060
Size: 380 154 163 1878 299 186
Fragments arranged by size:
1878 380 299 186 163 154
NcoI C'CATG_G
Cuts at: 0 686 1700 3060
Size: 686 1014 1360
Fragments arranged by size:
1360 1014 686
NsiI A_TGCA'T
Cuts at: 0 1739 1984 3060
Size: 1739 245 1076
Fragments arranged by size:
1739 1076 245
NspI r_CATG'y
Cuts at: 0 1311 1737 3060
Size: 1311 426 1323
Fragments arranged by size:
1323 1311 426
PasI CC'CwG_GG
Cuts at: 0 196 3060
Size: 196 2864
PflMI CCAn_nnn'nTGG
Cuts at: 0 316 501 2740 3060
Size: 316 185 2239 320
Fragments arranged by size:
2239 320 316 185
PfoI T'CCnGG_A
Cuts at: 0 1754 2780 3060
Size: 1754 1026 280
Fragments arranged by size:
1754 1026 280
PpuMI rG'GwC_Cy
Cuts at: 0 657 965 1265 2752 2784 2896 3060
Size: 657 308 300 1487 32 112 164
Fragments arranged by size:
1487 657 308 300 164 112 32
PshAI GACnn'nnGTC
Cuts at: 0 486 3060
Size: 486 2574
PspOMI G'GGCC_C
Cuts at: 0 1356 3060
Size: 1356 1704
PspXI vC'TCGA_Gb
Cuts at: 0 1361 3060
Size: 1361 1699
PstI C_TGCA'G
Cuts at: 0 488 551 605 746 907 1493 2506 2574
Size: 488 63 54 141 161 586 1013 68
Cuts at: 2574 3060
Size: 486
Fragments arranged by size:
1013 586 488 486 161 141 68 63
54
PvuII CAG'CTG
Cuts at: 0 697 2575 2874 3060
Size: 697 1878 299 186
Fragments arranged by size:
1878 697 299 186
SacI G_AGCT'C
Cuts at: 0 2705 3060
Size: 2705 355
SanDI GG'GwC_CC
Cuts at: 0 1265 3060
Size: 1265 1795
SexAI A'CCwGG_T
Cuts at: 0 494 1118 2310 3060
Size: 494 624 1192 750
Fragments arranged by size:
1192 750 624 494
SfcI C'TryA_G
Cuts at: 0 484 547 601 742 903 1230 1489 1812
Size: 484 63 54 141 161 327 259 323
Cuts at: 1812 2502 2570 3060
Size: 690 68 490
Fragments arranged by size:
690 490 484 327 323 259 161 141
68 63 54
SmaI CCC'GGG
Cuts at: 0 256 1796 3060
Size: 256 1540 1264
Fragments arranged by size:
1540 1264 256
SmlI C'TyrA_G
Cuts at: 0 1361 2049 3060
Size: 1361 688 1011
Fragments arranged by size:
1361 1011 688
SpeI A'CTAG_T
Cuts at: 0 1884 3060
Size: 1884 1176
SspI AAT'ATT
Cuts at: 0 1081 3060
Size: 1081 1979
StuI AGG'CCT
Cuts at: 0 463 3060
Size: 463 2597
StyI C'CwwG_G
Cuts at: 0 226 686 1700 1970 2111 2270 2324 3060
Size: 226 460 1014 270 141 159 54 736
Fragments arranged by size:
1014 736 460 270 226 159 141 54
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 188 2715 2834 3060
Size: 188 2527 119 226
Fragments arranged by size:
2527 226 188 119
TatI w'GTAC_w
Cuts at: 0 2603 3060
Size: 2603 457
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1516 1548 2614 2646 3060
Size: 1516 32 1066 32 414
Fragments arranged by size:
1516 1066 414 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 1195 3060
Size: 1195 1865
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 847 3060
Size: 847 2213
XhoI C'TCGA_G
Cuts at: 0 1361 3060
Size: 1361 1699
XmaI C'CCGG_G
Cuts at: 0 254 1794 3060
Size: 254 1540 1266
Fragments arranged by size:
1540 1266 254
Enzymes that do cut:
AarI AccI Acc65I AcuI AfeI AleI AloI AlwNI
ApaI ApaLI ApoI AseI AvaI BaeI BanI BanII
BbsI BcgI BclI BfrBI BglII BlpI Bme1580I BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaHI BsaXI BseRI
BseYI BsgI BsiHKAI BsiWI BsmBI Bsp1286I BspMI BsrBI
BsrDI BsrFI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI CspCI DraIII DrdI EaeI EarI EcoICRI Eco57MI
EcoNI EcoO109I FalI FspI FspAI HaeII Hin4I KpnI
MfeI MmeI MscI MslI MspA1I NcoI NsiI NspI
PasI PflMI PfoI PpuMI PshAI PspOMI PspXI PstI
PvuII SacI SanDI SexAI SfcI SmaI SmlI SpeI
SspI StuI StyI TaqII TatI TstI Tth111I XcmI
XhoI XmaI
Enzymes that do not cut:
AatII AclI AflII AflIII AgeI AhdI AlfI AscI
AsiSI AvrII BamHI BbeI BbvCI BciVI BglI BmgBI
BmtI BsaAI BsaBI BsaWI BsiEI BsmI BspEI BspHI
BsrGI BssHII BssSI BstAPI BstBI BstZ17I ClaI DraI
EagI EciI EcoRI EcoRV FseI HincII HindIII HpaI
KasI MluI NaeI NarI NdeI NgoMIV NheI NotI
NruI PacI PciI PmeI PmlI PpiI PsiI PsrI
PvuI RsrII SacII SalI SapI SbfI ScaI SfiI
SfoI SgfI SgrAI SnaBI SphI SrfI SwaI XbaI
XmnI ZraI