Restriction map for
pF1KB7323
(Linear) (Six-base) MAPSORT of: KIBB7323.seq Check: 9118 from: 1 to: 5862
KIBB7323.orf
With 182 enzymes: SgfI *
April 24, 2009 13:07 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 684 1087 1325 3295 3468 5862
Size: 684 403 238 1970 173 2394
Fragments arranged by size:
2394 1970 684 403 238 173
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 474 1414 2620 3050 3118 3375 4219 5510
Size: 474 940 1206 430 68 257 844 1291
Cuts at: 5510 5862
Size: 352
Fragments arranged by size:
1291 1206 940 844 474 430 352 257
68
AfeI AGC'GCT
Cuts at: 0 1741 5862
Size: 1741 4121
AflIII A'CryG_T
Cuts at: 0 2804 5862
Size: 2804 3058
AhdI GACnn_n'nnGTC
Cuts at: 0 72 5862
Size: 72 5790
AleI CACnn'nnGTG
Cuts at: 0 3478 4424 4648 5862
Size: 3478 946 224 1214
Fragments arranged by size:
3478 1214 946 224
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2212 3514 5862
Size: 2212 1302 2348
Fragments arranged by size:
2348 2212 1302
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 430 462 2368 2400 2551 2583 2641 2673
Size: 430 32 1906 32 151 32 58 32
Cuts at: 2673 5862
Size: 3189
Fragments arranged by size:
3189 1906 430 151 58 32 32 32
32
AlwNI CAG_nnn'CTG
Cuts at: 0 3564 4767 5523 5862
Size: 3564 1203 756 339
Fragments arranged by size:
3564 1203 756 339
ApaI G_GGCC'C
Cuts at: 0 10 2148 2252 3273 4601 5786 5800 5846
Size: 10 2138 104 1021 1328 1185 14 46
Cuts at: 5846 5862
Size: 16
Fragments arranged by size:
2138 1328 1185 1021 104 46 16 14
10
ApaLI G'TGCA_C
Cuts at: 0 1822 1863 5862
Size: 1822 41 3999
Fragments arranged by size:
3999 1822 41
ApoI r'AATT_y
Cuts at: 0 2452 5098 5862
Size: 2452 2646 764
Fragments arranged by size:
2646 2452 764
AvaI C'yCGr_G
Cuts at: 0 399 867 1083 1132 1342 3265 3299 3449
Size: 399 468 216 49 210 1923 34 150
Cuts at: 3449 4238 4472 4600 4741 4850 5862
Size: 789 234 128 141 109 1012
Fragments arranged by size:
1923 1012 789 468 399 234 216 210
150 141 128 109 49 34
BamHI G'GATC_C
Cuts at: 0 1172 5862
Size: 1172 4690
BanI G'GyrC_C
Cuts at: 0 16 565 696 2313 3119 3974 4418 5207
Size: 16 549 131 1617 806 855 444 789
Cuts at: 5207 5213 5862
Size: 6 649
Fragments arranged by size:
1617 855 806 789 649 549 444 131
16 6
BanII G_rGCy'C
Cuts at: 0 10 131 392 821 1439 1484 2148 2252
Size: 10 121 261 429 618 45 664 104
Cuts at: 2252 2483 2512 2837 3273 3859 4094 4601 4831
Size: 231 29 325 436 586 235 507 230
Cuts at: 4831 4851 5030 5312 5786 5800 5846 5862
Size: 20 179 282 474 14 46 16
Fragments arranged by size:
664 618 586 507 474 436 429 325
282 261 235 231 230 179 121 104
46 45 29 20 16 14 10
BbeI G_GCGC'C
Cuts at: 0 2317 5217 5862
Size: 2317 2900 645
Fragments arranged by size:
2900 2317 645
BbsI GAAGACnn'nnnn_
Cuts at: 0 1688 1958 2747 3056 5862
Size: 1688 270 789 309 2806
Fragments arranged by size:
2806 1688 789 309 270
BbvCI CC'TCA_GC
Cuts at: 0 1996 5015 5862
Size: 1996 3019 847
Fragments arranged by size:
3019 1996 847
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 928 962 5862
Size: 928 34 4900
Fragments arranged by size:
4900 928 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 5190 5862
Size: 5190 672
BclI T'GATC_A
Cuts at: 0 1832 5862
Size: 1832 4030
BglI GCCn_nnn'nGGC
Cuts at: 0 14 1971 3013 3268 4164 5485 5841 5862
Size: 14 1957 1042 255 896 1321 356 21
Fragments arranged by size:
1957 1321 1042 896 356 255 21 14
BlpI GC'TnA_GC
Cuts at: 0 2854 4957 5602 5862
Size: 2854 2103 645 260
Fragments arranged by size:
2854 2103 645 260
Bme1580I G_kGCm'C
Cuts at: 0 10 19 701 1826 1867 2148 2155 2252
Size: 10 9 682 1125 41 281 7 97
Cuts at: 2252 3122 3273 4472 4601 4655 4749 5786 5800
Size: 870 151 1199 129 54 94 1037 14
Cuts at: 5800 5846 5862
Size: 46 16
Fragments arranged by size:
1199 1125 1037 870 682 281 151 129
97 94 54 46 41 16 14 10
9 7
BmgBI CAC'GTC
Cuts at: 0 435 1476 2805 3612 3744 5622 5862
Size: 435 1041 1329 807 132 1878 240
Fragments arranged by size:
1878 1329 1041 807 435 240 132
BmrI ACTGGGnnnn_n'
Cuts at: 0 4 1932 2298 3966 5862
Size: 4 1928 366 1668 1896
Fragments arranged by size:
1928 1896 1668 366 4
BmtI G_CTAG'C
Cuts at: 0 679 5862
Size: 679 5183
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2256 2376 4500 4644 5862
Size: 2256 120 2124 144 1218
Fragments arranged by size:
2256 2124 1218 144 120
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 770 1141 1443 1528 1612 2134 2666 2668
Size: 770 371 302 85 84 522 532 2
Cuts at: 2668 2722 2734 3187 3199 4470 4972 5365 5407
Size: 54 12 453 12 1271 502 393 42
Cuts at: 5407 5611 5862
Size: 204 251
Fragments arranged by size:
1271 770 532 522 502 453 393 371
302 251 204 85 84 54 42 12
12 2
Bpu10I CC'TnA_GC
Cuts at: 0 1499 1996 2785 3853 5015 5698 5862
Size: 1499 497 789 1068 1162 683 164
Fragments arranged by size:
1499 1162 1068 789 683 497 164
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 151 292 805 1894 1963 2539 2779 3562
Size: 151 141 513 1089 69 576 240 783
Cuts at: 3562 5862
Size: 2300
Fragments arranged by size:
2300 1089 783 576 513 240 151 141
69
BsaI GGTCTCn'nnnn_
Cuts at: 0 4444 5862
Size: 4444 1418
BsaAI yAC'GTr
Cuts at: 0 2328 2460 3879 4497 5862
Size: 2328 132 1419 618 1365
Fragments arranged by size:
2328 1419 1365 618 132
BsaHI Gr'CG_yC
Cuts at: 0 1295 2314 4334 5214 5862
Size: 1295 1019 2020 880 648
Fragments arranged by size:
2020 1295 1019 880 648
BsaWI w'CCGG_w
Cuts at: 0 3800 5417 5862
Size: 3800 1617 445
Fragments arranged by size:
3800 1617 445
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 379 409 910 940 2065 2095 2365 2395
Size: 379 30 501 30 1125 30 270 30
Cuts at: 2395 2641 2671 3841 3871 4045 4075 5380 5410
Size: 246 30 1170 30 174 30 1305 30
Cuts at: 5410 5801 5831 5862
Size: 391 30 31
Fragments arranged by size:
1305 1170 1125 501 391 379 270 246
174 31 30 30 30 30 30 30
30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 37 40 64 469 855 904 987 1222
Size: 37 3 24 405 386 49 83 235
Cuts at: 1222 1225 1240 1243 1246 1296 1750 2656 2665
Size: 3 15 3 3 50 454 906 9
Cuts at: 2665 2731 3280 3478 3904 3919 3949 4620 4870
Size: 66 549 198 426 15 30 671 250
Cuts at: 4870 4882 4927 5032 5179 5422 5608 5745 5862
Size: 12 45 105 147 243 186 137 117
Fragments arranged by size:
906 671 549 454 426 405 386 250
243 235 198 186 147 137 117 105
83 66 50 49 45 37 30 24
15 15 12 9 3 3 3 3
BseYI C'CCAG_C
Cuts at: 0 701 1483 1944 2769 3272 4263 4313 4686
Size: 701 782 461 825 503 991 50 373
Cuts at: 4686 4712 4716 4830 5542 5862
Size: 26 4 114 712 320
Fragments arranged by size:
991 825 782 712 701 503 461 373
320 114 50 26 4
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2083 2218 2464 4466 5554 5862
Size: 2083 135 246 2002 1088 308
Fragments arranged by size:
2083 2002 1088 308 246 135
BsiEI CG_ry'CG
Cuts at: 0 815 5862
Size: 815 5047
BsiHKAI G_wGCw'C
Cuts at: 0 1826 1867 2483 2837 3131 3859 4094 5862
Size: 1826 41 616 354 294 728 235 1768
Fragments arranged by size:
1826 1768 728 616 354 294 235 41
BsmI GAATG_Cn'
Cuts at: 0 946 5862
Size: 946 4916
BsmBI CGTCTCn'nnnn_
Cuts at: 0 646 4864 5862
Size: 646 4218 998
Fragments arranged by size:
4218 998 646
Bsp1286I G_dGCh'C
Cuts at: 0 10 19 131 392 701 821 1439 1484
Size: 10 9 112 261 309 120 618 45
Cuts at: 1484 1826 1867 2148 2155 2252 2483 2512 2837
Size: 342 41 281 7 97 231 29 325
Cuts at: 2837 3122 3131 3273 3859 4094 4472 4601 4655
Size: 285 9 142 586 235 378 129 54
Cuts at: 4655 4749 4831 4851 5030 5312 5786 5800 5846
Size: 94 82 20 179 282 474 14 46
Cuts at: 5846 5862
Size: 16
Fragments arranged by size:
618 586 474 378 342 325 309 285
282 281 261 235 231 179 142 129
120 112 97 94 82 54 46 45
41 29 20 16 14 10 9 9
7
BspEI T'CCGG_A
Cuts at: 0 5417 5862
Size: 5417 445
BspHI T'CATG_A
Cuts at: 0 2954 5862
Size: 2954 2908
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 684 1087 1325 1856 2774 3295 3468 4032
Size: 684 403 238 531 918 521 173 564
Cuts at: 4032 5363 5862
Size: 1331 499
Fragments arranged by size:
1331 918 684 564 531 521 499 403
238 173
BsrBI CCG'CTC
Cuts at: 0 2360 3204 3915 4860 5169 5862
Size: 2360 844 711 945 309 693
Fragments arranged by size:
2360 945 844 711 693 309
BsrDI GCAATG_nn'
Cuts at: 0 219 512 1320 1563 5862
Size: 219 293 808 243 4299
Fragments arranged by size:
4299 808 293 243 219
BsrFI r'CCGG_y
Cuts at: 0 2820 3642 5210 5862
Size: 2820 822 1568 652
Fragments arranged by size:
2820 1568 822 652
BsrGI T'GTAC_A
Cuts at: 0 5765 5862
Size: 5765 97
BssHII G'CGCG_C
Cuts at: 0 2264 5862
Size: 2264 3598
BssSI C'ACGA_G
Cuts at: 0 4822 5862
Size: 4822 1040
BstAPI GCAn_nnn'nTGC
Cuts at: 0 2766 3126 4497 5862
Size: 2766 360 1371 1365
Fragments arranged by size:
2766 1371 1365 360
BstEII G'GTnAC_C
Cuts at: 0 657 2331 5862
Size: 657 1674 3531
Fragments arranged by size:
3531 1674 657
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1054 3156 3795 5862
Size: 1054 2102 639 2067
Fragments arranged by size:
2102 2067 1054 639
BstYI r'GATC_y
Cuts at: 0 240 533 1172 1346 4937 5862
Size: 240 293 639 174 3591 925
Fragments arranged by size:
3591 925 639 293 240 174
Bsu36I CC'TnA_GG
Cuts at: 0 502 4592 5862
Size: 502 4090 1270
Fragments arranged by size:
4090 1270 502
BtgI C'CryG_G
Cuts at: 0 34 614 2045 2068 3008 3329 5287 5465
Size: 34 580 1431 23 940 321 1958 178
Cuts at: 5465 5862
Size: 397
Fragments arranged by size:
1958 1431 940 580 397 321 178 34
23
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 5103 5862
Size: 5103 759
BtsI GCAGTG_nn'
Cuts at: 0 276 2095 3762 5862
Size: 276 1819 1667 2100
Fragments arranged by size:
2100 1819 1667 276
ClaI AT'CG_AT
Cuts at: 0 1877 5862
Size: 1877 3985
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1303 1338 5862
Size: 1303 35 4524
Fragments arranged by size:
4524 1303 35
DraIII CAC_nnn'GTG
Cuts at: 0 1608 3126 3513 5153 5862
Size: 1608 1518 387 1640 709
Fragments arranged by size:
1640 1608 1518 709 387
DrdI GACnn_nn'nnGTC
Cuts at: 0 2047 5862
Size: 2047 3815
EaeI y'GGCC_r
Cuts at: 0 413 812 2168 2822 3326 3411 3482 3689
Size: 413 399 1356 654 504 85 71 207
Cuts at: 3689 3779 4861 5663 5862
Size: 90 1082 802 199
Fragments arranged by size:
1356 1082 802 654 504 413 399 207
199 90 85 71
EagI C'GGCC_G
Cuts at: 0 812 5862
Size: 812 5050
EarI CTCTTCn'nnn_
Cuts at: 0 230 242 958 982 1199 1693 2998 3604
Size: 230 12 716 24 217 494 1305 606
Cuts at: 3604 4145 4195 4369 4414 5862
Size: 541 50 174 45 1448
Fragments arranged by size:
1448 1305 716 606 541 494 230 217
174 50 45 24 12
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 3349 4453 5862
Size: 3349 1104 1409
Fragments arranged by size:
3349 1409 1104
EcoICRI GAG'CTC
Cuts at: 0 2481 2835 3857 4092 5862
Size: 2481 354 1022 235 1770
Fragments arranged by size:
2481 1770 1022 354 235
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 474 770 1141 1414 1443 1528 1612 2134
Size: 474 296 371 273 29 85 84 522
Cuts at: 2134 2620 2666 2668 2722 2734 3050 3118 3187
Size: 486 46 2 54 12 316 68 69
Cuts at: 3187 3199 3375 4219 4470 4972 5365 5407 5510
Size: 12 176 844 251 502 393 42 103
Cuts at: 5510 5611 5862
Size: 101 251
Fragments arranged by size:
844 522 502 486 474 393 371 316
296 273 251 251 176 103 101 85
84 69 68 54 46 42 29 12
12 2
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1083 1912 2227 3166 3235 4018 5527 5572
Size: 1083 829 315 939 69 783 1509 45
Cuts at: 5572 5862
Size: 290
Fragments arranged by size:
1509 1083 939 829 783 315 290 69
45
EcoO109I rG'GnC_Cy
Cuts at: 0 6 423 745 1088 1468 1713 2118 2248
Size: 6 417 322 343 380 245 405 130
Cuts at: 2248 2249 2627 3015 3228 3269 3999 4362 4479
Size: 1 378 388 213 41 730 363 117
Cuts at: 4479 4597 4779 4881 5448 5577 5724 5796 5797
Size: 118 182 102 567 129 147 72 1
Cuts at: 5797 5842 5843 5862
Size: 45 1 19
Fragments arranged by size:
730 567 417 405 388 380 378 363
343 322 245 213 182 147 130 129
118 117 102 72 45 41 19 6
1 1 1
EcoRV GAT'ATC
Cuts at: 0 5859 5862
Size: 5859 3
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 846 5862
Size: 846 5016
HaeII r_GCGC'y
Cuts at: 0 590 911 1743 2317 5217 5747 5862
Size: 590 321 832 574 2900 530 115
Fragments arranged by size:
2900 832 590 574 530 321 115
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 58 90 208 240 379 411 1654 1686
Size: 58 32 118 32 139 32 1243 32
Cuts at: 1686 3034 3066 3652 3684 4078 4090 4110 4122
Size: 1348 32 586 32 394 12 20 12
Cuts at: 4122 5801 5833 5862
Size: 1679 32 29
Fragments arranged by size:
1679 1348 1243 586 394 139 118 58
32 32 32 32 32 32 32 29
20 12 12
HincII GTy'rAC
Cuts at: 0 4047 4560 5820 5862
Size: 4047 513 1260 42
Fragments arranged by size:
4047 1260 513 42
KasI G'GCGC_C
Cuts at: 0 2313 5213 5862
Size: 2313 2900 649
Fragments arranged by size:
2900 2313 649
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 236 875 5663 5862
Size: 236 639 4788 199
Fragments arranged by size:
4788 639 236 199
MscI TGG'CCA
Cuts at: 0 3328 3484 5862
Size: 3328 156 2378
Fragments arranged by size:
3328 2378 156
MslI CAynn'nnrTG
Cuts at: 0 1609 2956 3478 3980 4258 4424 4648 5862
Size: 1609 1347 522 502 278 166 224 1214
Fragments arranged by size:
1609 1347 1214 522 502 278 224 166
MspA1I CmG'CkG
Cuts at: 0 36 616 625 1338 1410 1590 2112 4197
Size: 36 580 9 713 72 180 522 2085
Cuts at: 4197 4284 4686 4716 4902 5264 5589 5862
Size: 87 402 30 186 362 325 273
Fragments arranged by size:
2085 713 580 522 402 362 325 273
186 180 87 72 36 30 9
NaeI GCC'GGC
Cuts at: 0 2822 5862
Size: 2822 3040
NarI GG'CG_CC
Cuts at: 0 2314 5214 5862
Size: 2314 2900 648
Fragments arranged by size:
2900 2314 648
NcoI C'CATG_G
Cuts at: 0 2068 5465 5862
Size: 2068 3397 397
Fragments arranged by size:
3397 2068 397
NgoMIV G'CCGG_C
Cuts at: 0 2820 5862
Size: 2820 3042
NheI G'CTAG_C
Cuts at: 0 675 5862
Size: 675 5187
NspI r_CATG'y
Cuts at: 0 2784 2820 4323 4558 5862
Size: 2784 36 1503 235 1304
Fragments arranged by size:
2784 1503 1304 235 36
PasI CC'CwG_GG
Cuts at: 0 954 2148 5838 5862
Size: 954 1194 3690 24
Fragments arranged by size:
3690 1194 954 24
PflMI CCAn_nnn'nTGG
Cuts at: 0 2519 3513 4353 5862
Size: 2519 994 840 1509
Fragments arranged by size:
2519 1509 994 840
PfoI T'CCnGG_A
Cuts at: 0 2711 3164 3176 4186 4940 5862
Size: 2711 453 12 1010 754 922
Fragments arranged by size:
2711 1010 922 754 453 12
PmlI CAC'GTG
Cuts at: 0 2460 4497 5862
Size: 2460 2037 1365
Fragments arranged by size:
2460 2037 1365
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1513 1545 2641 2673 5862
Size: 1513 32 1096 32 3189
Fragments arranged by size:
3189 1513 1096 32 32
PpuMI rG'GwC_Cy
Cuts at: 0 1713 2627 3999 4362 5448 5577 5724 5862
Size: 1713 914 1372 363 1086 129 147 138
Fragments arranged by size:
1713 1372 1086 914 363 147 138 129
PspOMI G'GGCC_C
Cuts at: 0 6 2144 2248 3269 4597 5782 5796 5842
Size: 6 2138 104 1021 1328 1185 14 46
Cuts at: 5842 5862
Size: 20
Fragments arranged by size:
2138 1328 1185 1021 104 46 20 14
6
PspXI vC'TCGA_Gb
Cuts at: 0 867 5862
Size: 867 4995
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 2866 2898 5862
Size: 2866 32 2964
Fragments arranged by size:
2964 2866 32
PstI C_TGCA'G
Cuts at: 0 951 5678 5862
Size: 951 4727 184
Fragments arranged by size:
4727 951 184
PvuII CAG'CTG
Cuts at: 0 1590 2112 4197 4716 4902 5589 5862
Size: 1590 522 2085 519 186 687 273
Fragments arranged by size:
2085 1590 687 522 519 273 186
SacI G_AGCT'C
Cuts at: 0 2483 2837 3859 4094 5862
Size: 2483 354 1022 235 1768
Fragments arranged by size:
2483 1768 1022 354 235
SacII CC_GC'GG
Cuts at: 0 37 617 5862
Size: 37 580 5245
Fragments arranged by size:
5245 580 37
SanDI GG'GwC_CC
Cuts at: 0 2627 5724 5862
Size: 2627 3097 138
Fragments arranged by size:
3097 2627 138
SapI GCTCTTCn'nnn_
Cuts at: 0 958 4369 5862
Size: 958 3411 1493
Fragments arranged by size:
3411 1493 958
ScaI AGT'ACT
Cuts at: 0 2875 5862
Size: 2875 2987
SexAI A'CCwGG_T
Cuts at: 0 2255 3509 5862
Size: 2255 1254 2353
Fragments arranged by size:
2353 2255 1254
SfcI C'TryA_G
Cuts at: 0 647 947 1585 3085 3815 3832 5674 5862
Size: 647 300 638 1500 730 17 1842 188
Fragments arranged by size:
1842 1500 730 647 638 300 188 17
SfoI GGC'GCC
Cuts at: 0 2315 5215 5862
Size: 2315 2900 647
Fragments arranged by size:
2900 2315 647
SgrAI Cr'CCGG_yG
Cuts at: 0 5210 5862
Size: 5210 652
SmaI CCC'GGG
Cuts at: 0 3267 3301 4474 4743 5862
Size: 3267 34 1173 269 1119
Fragments arranged by size:
3267 1173 1119 269 34
SmlI C'TyrA_G
Cuts at: 0 166 307 820 867 1909 1978 2554 2794
Size: 166 141 513 47 1042 69 576 240
Cuts at: 2794 3577 5862
Size: 783 2285
Fragments arranged by size:
2285 1042 783 576 513 240 166 141
69 47
SpeI A'CTAG_T
Cuts at: 0 4524 5862
Size: 4524 1338
SphI G_CATG'C
Cuts at: 0 4323 5862
Size: 4323 1539
SspI AAT'ATT
Cuts at: 0 4114 5862
Size: 4114 1748
StuI AGG'CCT
Cuts at: 0 487 1918 3388 4291 5862
Size: 487 1431 1470 903 1571
Fragments arranged by size:
1571 1470 1431 903 487
StyI C'CwwG_G
Cuts at: 0 731 2068 2735 2918 4756 4793 5009 5465
Size: 731 1337 667 183 1838 37 216 456
Cuts at: 5465 5528 5862
Size: 63 334
Fragments arranged by size:
1838 1337 731 667 456 334 216 183
63 37
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 553 1563 2845 3520 3666 5862
Size: 553 1010 1282 675 146 2196
Fragments arranged by size:
2196 1282 1010 675 553 146
TatI w'GTAC_w
Cuts at: 0 1378 2873 4568 5765 5862
Size: 1378 1495 1695 1197 97
Fragments arranged by size:
1695 1495 1378 1197 97
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 163 195 910 942 3198 3230 4063 4095
Size: 163 32 715 32 2256 32 833 32
Cuts at: 4095 5862
Size: 1767
Fragments arranged by size:
2256 1767 833 715 163 32 32 32
32
Tth111I GACn'n_nGTC
Cuts at: 0 3340 5862
Size: 3340 2522
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1358 3561 5862
Size: 1358 2203 2301
Fragments arranged by size:
2301 2203 1358
XhoI C'TCGA_G
Cuts at: 0 867 5862
Size: 867 4995
XmaI C'CCGG_G
Cuts at: 0 3265 3299 4472 4741 5862
Size: 3265 34 1173 269 1121
Fragments arranged by size:
3265 1173 1121 269 34
XmnI GAAnn'nnTTC
Cuts at: 0 2422 5359 5862
Size: 2422 2937 503
Fragments arranged by size:
2937 2422 503
Enzymes that do cut:
AarI AcuI AfeI AflIII AhdI AleI AlfI AloI
AlwNI ApaI ApaLI ApoI AvaI BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BglI BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRV FalI HaeII Hin4I HincII KasI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NheI NspI PasI PflMI PfoI PmlI PpiI PpuMI
PspOMI PspXI PsrI PstI PvuII SacI SacII SanDI
SapI ScaI SexAI SfcI SfoI SgrAI SmaI SmlI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AflII AgeI AscI AseI
AsiSI AvrII BaeI BfrBI BglII BsaBI BsiWI BstBI
BstZ17I DraI EcoRI FseI FspI FspAI HindIII HpaI
KpnI MfeI MluI NdeI NotI NruI NsiI PacI
PciI PmeI PshAI PsiI PvuI RsrII SalI SbfI
SfiI SgfI SnaBI SrfI SwaI XbaI ZraI