Restriction map for
pF1KB7312
(Linear) (Six-base) MAPSORT of: KIBB7312.seq Check: 5624 from: 1 to: 3177
KIBB7312.orf
With 182 enzymes: SgfI *
April 24, 2009 13:07 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 2511 3177
Size: 2511 666
AatII G_ACGT'C
Cuts at: 0 2615 3177
Size: 2615 562
AccI GT'mk_AC
Cuts at: 0 2039 2615 3177
Size: 2039 576 562
Fragments arranged by size:
2039 576 562
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1582 3177
Size: 1582 1595
AhdI GACnn_n'nnGTC
Cuts at: 0 1171 3177
Size: 1171 2006
AleI CACnn'nnGTG
Cuts at: 0 391 680 1648 3177
Size: 391 289 968 1529
Fragments arranged by size:
1529 968 391 289
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2989 3177
Size: 2989 188
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 460 492 3177
Size: 460 32 2685
Fragments arranged by size:
2685 460 32
AlwNI CAG_nnn'CTG
Cuts at: 0 300 1068 3177
Size: 300 768 2109
Fragments arranged by size:
2109 768 300
ApaI G_GGCC'C
Cuts at: 0 819 1668 2068 3177
Size: 819 849 400 1109
Fragments arranged by size:
1109 849 819 400
AvaI C'yCGr_G
Cuts at: 0 630 1265 1505 1924 2292 2809 3015 3131
Size: 630 635 240 419 368 517 206 116
Cuts at: 3131 3177
Size: 46
Fragments arranged by size:
635 630 517 419 368 240 206 116
46
AvrII C'CTAG_G
Cuts at: 0 2646 3177
Size: 2646 531
BamHI G'GATC_C
Cuts at: 0 338 1289 2189 3177
Size: 338 951 900 988
Fragments arranged by size:
988 951 900 338
BanI G'GyrC_C
Cuts at: 0 1542 2457 2540 3177
Size: 1542 915 83 637
Fragments arranged by size:
1542 915 637 83
BanII G_rGCy'C
Cuts at: 0 8 407 819 1187 1390 1668 1708 1931
Size: 8 399 412 368 203 278 40 223
Cuts at: 1931 2068 2816 2866 3053 3177
Size: 137 748 50 187 124
Fragments arranged by size:
748 412 399 368 278 223 203 187
137 124 50 40 8
BbeI G_GCGC'C
Cuts at: 0 2461 2544 3177
Size: 2461 83 633
Fragments arranged by size:
2461 633 83
BbvCI CC'TCA_GC
Cuts at: 0 787 2923 3177
Size: 787 2136 254
Fragments arranged by size:
2136 787 254
BglI GCCn_nnn'nGGC
Cuts at: 0 2419 3177
Size: 2419 758
BlpI GC'TnA_GC
Cuts at: 0 301 401 3177
Size: 301 100 2776
Fragments arranged by size:
2776 301 100
Bme1580I G_kGCm'C
Cuts at: 0 35 819 1547 1668 1678 2068 3177
Size: 35 784 728 121 10 390 1109
Fragments arranged by size:
1109 784 728 390 121 35 10
BmgBI CAC'GTC
Cuts at: 0 876 2745 3177
Size: 876 1869 432
Fragments arranged by size:
1869 876 432
BmrI ACTGGGnnnn_n'
Cuts at: 0 1455 2947 3083 3177
Size: 1455 1492 136 94
Fragments arranged by size:
1492 1455 136 94
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 555 1203 3177
Size: 555 648 1974
Fragments arranged by size:
1974 648 555
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 277 280 334 609 907 1315 1807 2215
Size: 277 3 54 275 298 408 492 408
Cuts at: 2215 2704 2776 3177
Size: 489 72 401
Fragments arranged by size:
492 489 408 408 401 298 277 275
72 54 3
Bpu10I CC'TnA_GC
Cuts at: 0 787 1460 2138 2662 2923 3177
Size: 787 673 678 524 261 254
Fragments arranged by size:
787 678 673 524 261 254
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 985 3177
Size: 985 2192
BsaI GGTCTCn'nnnn_
Cuts at: 0 217 3177
Size: 217 2960
BsaAI yAC'GTr
Cuts at: 0 168 771 1282 3177
Size: 168 603 511 1895
Fragments arranged by size:
1895 603 511 168
BsaHI Gr'CG_yC
Cuts at: 0 250 1248 1942 2398 2411 2458 2541 2612
Size: 250 998 694 456 13 47 83 71
Cuts at: 2612 3177
Size: 565
Fragments arranged by size:
998 694 565 456 250 83 71 47
13
BsaWI w'CCGG_w
Cuts at: 0 197 1885 3177
Size: 197 1688 1292
Fragments arranged by size:
1688 1292 197
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 352 382 628 658 1323 1353 1580 1610
Size: 352 30 246 30 665 30 227 30
Cuts at: 1610 1975 2005 2188 2218 2500 2530 3177
Size: 365 30 183 30 282 30 647
Fragments arranged by size:
665 647 365 352 282 246 227 183
30 30 30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 331 334 1195 2095 2911 3177
Size: 331 3 861 900 816 266
Fragments arranged by size:
900 861 816 331 266 3
BseYI C'CCAG_C
Cuts at: 0 964 989 1220 1357 2120 2210 2305 2435
Size: 964 25 231 137 763 90 95 130
Cuts at: 2435 2858 3025 3086 3177
Size: 423 167 61 91
Fragments arranged by size:
964 763 423 231 167 137 130 95
91 90 61 25
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 256 1723 2977 3177
Size: 256 1467 1254 200
Fragments arranged by size:
1467 1254 256 200
BsiEI CG_ry'CG
Cuts at: 0 182 866 915 2465 2912 3177
Size: 182 684 49 1550 447 265
Fragments arranged by size:
1550 684 447 265 182 49
BsiHKAI G_wGCw'C
Cuts at: 0 362 407 677 1931 1982 2380 3177
Size: 362 45 270 1254 51 398 797
Fragments arranged by size:
1254 797 398 362 270 51 45
Bsp1286I G_dGCh'C
Cuts at: 0 8 35 362 407 677 819 1187 1390
Size: 8 27 327 45 270 142 368 203
Cuts at: 1390 1547 1668 1678 1708 1931 1982 2068 2380
Size: 157 121 10 30 223 51 86 312
Cuts at: 2380 2816 2866 3053 3177
Size: 436 50 187 124
Fragments arranged by size:
436 368 327 312 270 223 203 187
157 142 124 121 86 51 50 45
30 27 10 8
BspEI T'CCGG_A
Cuts at: 0 1885 3177
Size: 1885 1292
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1542 1647 2358 2511 2944 3177
Size: 1542 105 711 153 433 233
Fragments arranged by size:
1542 711 433 233 153 105
BsrBI CCG'CTC
Cuts at: 0 58 82 2666 3177
Size: 58 24 2584 511
Fragments arranged by size:
2584 511 58 24
BsrDI GCAATG_nn'
Cuts at: 0 1496 3177
Size: 1496 1681
BsrFI r'CCGG_y
Cuts at: 0 598 914 1193 2460 2769 2854 2983 3104
Size: 598 316 279 1267 309 85 129 121
Cuts at: 3104 3177
Size: 73
Fragments arranged by size:
1267 598 316 309 279 129 121 85
73
BsrGI T'GTAC_A
Cuts at: 0 848 2159 3177
Size: 848 1311 1018
Fragments arranged by size:
1311 1018 848
BssHII G'CGCG_C
Cuts at: 0 2695 3177
Size: 2695 482
BssSI C'ACGA_G
Cuts at: 0 28 3177
Size: 28 3149
BstAPI GCAn_nnn'nTGC
Cuts at: 0 279 974 1072 2012 2843 3177
Size: 279 695 98 940 831 334
Fragments arranged by size:
940 831 695 334 279 98
BstEII G'GTnAC_C
Cuts at: 0 1449 3177
Size: 1449 1728
BstXI CCAn_nnnn'nTGG
Cuts at: 0 711 1803 2481 3177
Size: 711 1092 678 696
Fragments arranged by size:
1092 711 696 678
BstYI r'GATC_y
Cuts at: 0 338 1289 2189 3177
Size: 338 951 900 988
Fragments arranged by size:
988 951 900 338
BtgI C'CryG_G
Cuts at: 0 643 1334 1946 2502 2515 2885 3177
Size: 643 691 612 556 13 370 292
Fragments arranged by size:
691 643 612 556 370 292 13
BtsI GCAGTG_nn'
Cuts at: 0 265 561 726 1629 1676 2381 2601 2638
Size: 265 296 165 903 47 705 220 37
Cuts at: 2638 3177
Size: 539
Fragments arranged by size:
903 705 539 296 265 220 165 47
37
ClaI AT'CG_AT
Cuts at: 0 1414 3177
Size: 1414 1763
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 93 128 1580 1615 3177
Size: 93 35 1452 35 1562
Fragments arranged by size:
1562 1452 93 35 35
DraIII CAC_nnn'GTG
Cuts at: 0 30 1072 3177
Size: 30 1042 2105
Fragments arranged by size:
2105 1042 30
DrdI GACnn_nn'nnGTC
Cuts at: 0 1015 1780 3177
Size: 1015 765 1397
Fragments arranged by size:
1397 1015 765
EaeI y'GGCC_r
Cuts at: 0 83 101 179 596 863 912 1086 1191
Size: 83 18 78 417 267 49 174 105
Cuts at: 1191 1793 1844 2252 2462 2512 2909 3102 3177
Size: 602 51 408 210 50 397 193 75
Fragments arranged by size:
602 417 408 397 267 210 193 174
105 83 78 75 51 50 49 18
EagI C'GGCC_G
Cuts at: 0 179 863 912 2462 2909 3177
Size: 179 684 49 1550 447 268
Fragments arranged by size:
1550 684 447 268 179 49
EcoICRI GAG'CTC
Cuts at: 0 405 1929 3177
Size: 405 1524 1248
Fragments arranged by size:
1524 1248 405
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 277 280 334 609 907 1315 1582 1807
Size: 277 3 54 275 298 408 267 225
Cuts at: 1807 2215 2704 2776 3177
Size: 408 489 72 401
Fragments arranged by size:
489 408 408 401 298 277 275 267
225 72 54 3
EcoNI CCTnn'n_nnAGG
Cuts at: 0 316 1003 1936 3177
Size: 316 687 933 1241
Fragments arranged by size:
1241 933 687 316
EcoO109I rG'GnC_Cy
Cuts at: 0 424 1165 1422 1571 2064 2065 2085 2622
Size: 424 741 257 149 493 1 20 537
Cuts at: 2622 3031 3177
Size: 409 146
Fragments arranged by size:
741 537 493 424 409 257 149 146
20 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 444 673 693 1026 3177
Size: 444 229 20 333 2151
Fragments arranged by size:
2151 444 333 229 20
FseI GG_CCGG'CC
Cuts at: 0 3108 3177
Size: 3108 69
FspI TGC'GCA
Cuts at: 0 2016 3177
Size: 2016 1161
HaeII r_GCGC'y
Cuts at: 0 2461 2544 2752 3177
Size: 2461 83 208 425
Fragments arranged by size:
2461 425 208 83
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 433 465 885 917 1157 1189 2188 2220
Size: 433 32 420 32 240 32 999 32
Cuts at: 2220 2998 3030 3152 3177
Size: 778 32 122 25
Fragments arranged by size:
999 778 433 420 240 122 32 32
32 32 32 25
HincII GTy'rAC
Cuts at: 0 2040 3177
Size: 2040 1137
KasI G'GCGC_C
Cuts at: 0 2457 2540 3177
Size: 2457 83 637
Fragments arranged by size:
2457 637 83
MfeI C'AATT_G
Cuts at: 0 389 3177
Size: 389 2788
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 575 3177
Size: 575 2602
MscI TGG'CCA
Cuts at: 0 103 1795 1846 2254 3177
Size: 103 1692 51 408 923
Fragments arranged by size:
1692 923 408 103 51
MslI CAynn'nnrTG
Cuts at: 0 94 391 680 1648 2245 3177
Size: 94 297 289 968 597 932
Fragments arranged by size:
968 932 597 297 289 94
MspA1I CmG'CkG
Cuts at: 0 458 791 1610 2278 2497 2682 2898 3011
Size: 458 333 819 668 219 185 216 113
Cuts at: 3011 3142 3177
Size: 131 35
Fragments arranged by size:
819 668 458 333 219 216 185 131
113 35
NaeI GCC'GGC
Cuts at: 0 600 916 2462 2985 3106 3177
Size: 600 316 1546 523 121 71
Fragments arranged by size:
1546 600 523 316 121 71
NarI GG'CG_CC
Cuts at: 0 2458 2541 3177
Size: 2458 83 636
Fragments arranged by size:
2458 636 83
NcoI C'CATG_G
Cuts at: 0 1334 2885 3177
Size: 1334 1551 292
Fragments arranged by size:
1551 1334 292
NgoMIV G'CCGG_C
Cuts at: 0 598 914 2460 2983 3104 3177
Size: 598 316 1546 523 121 73
Fragments arranged by size:
1546 598 523 316 121 73
NspI r_CATG'y
Cuts at: 0 1320 2976 3177
Size: 1320 1656 201
Fragments arranged by size:
1656 1320 201
PasI CC'CwG_GG
Cuts at: 0 126 2320 3177
Size: 126 2194 857
Fragments arranged by size:
2194 857 126
PflMI CCAn_nnn'nTGG
Cuts at: 0 2449 2633 3177
Size: 2449 184 544
Fragments arranged by size:
2449 544 184
PfoI T'CCnGG_A
Cuts at: 0 1292 1763 1784 2024 2192 3170 3177
Size: 1292 471 21 240 168 978 7
Fragments arranged by size:
1292 978 471 240 168 21 7
PmlI CAC'GTG
Cuts at: 0 168 3177
Size: 168 3009
PpuMI rG'GwC_Cy
Cuts at: 0 424 1165 1571 2622 3031 3177
Size: 424 741 406 1051 409 146
Fragments arranged by size:
1051 741 424 409 406 146
PspOMI G'GGCC_C
Cuts at: 0 815 1664 2064 3177
Size: 815 849 400 1113
Fragments arranged by size:
1113 849 815 400
PstI C_TGCA'G
Cuts at: 0 1037 1937 2903 3046 3177
Size: 1037 900 966 143 131
Fragments arranged by size:
1037 966 900 143 131
PvuII CAG'CTG
Cuts at: 0 2278 2682 2898 3011 3142 3177
Size: 2278 404 216 113 131 35
Fragments arranged by size:
2278 404 216 131 113 35
SacI G_AGCT'C
Cuts at: 0 407 1931 3177
Size: 407 1524 1246
Fragments arranged by size:
1524 1246 407
SalI G'TCGA_C
Cuts at: 0 2038 3177
Size: 2038 1139
SanDI GG'GwC_CC
Cuts at: 0 424 1571 2622 3031 3177
Size: 424 1147 1051 409 146
Fragments arranged by size:
1147 1051 424 409 146
SbfI CC_TGCA'GG
Cuts at: 0 3046 3177
Size: 3046 131
SfcI C'TryA_G
Cuts at: 0 1033 1933 1971 2899 3042 3177
Size: 1033 900 38 928 143 135
Fragments arranged by size:
1033 928 900 143 135 38
SfoI GGC'GCC
Cuts at: 0 2459 2542 3177
Size: 2459 83 635
Fragments arranged by size:
2459 635 83
SmaI CCC'GGG
Cuts at: 0 632 1507 2294 3133 3177
Size: 632 875 787 839 44
Fragments arranged by size:
875 839 787 632 44
SmlI C'TyrA_G
Cuts at: 0 1000 3177
Size: 1000 2177
SphI G_CATG'C
Cuts at: 0 1320 2976 3177
Size: 1320 1656 201
Fragments arranged by size:
1656 1320 201
StuI AGG'CCT
Cuts at: 0 1485 3177
Size: 1485 1692
StyI C'CwwG_G
Cuts at: 0 44 428 503 1334 2646 2885 3177
Size: 44 384 75 831 1312 239 292
Fragments arranged by size:
1312 831 384 292 239 75 44
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 750 763 978 2319 2449 3054 3177
Size: 750 13 215 1341 130 605 123
Fragments arranged by size:
1341 750 605 215 130 123 13
TatI w'GTAC_w
Cuts at: 0 848 2159 3177
Size: 848 1311 1018
Fragments arranged by size:
1311 1018 848
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 55 87 146 178 352 384 628 660
Size: 55 32 59 32 174 32 244 32
Cuts at: 660 941 973 1323 1355 1580 1612 2500 2532
Size: 281 32 350 32 225 32 888 32
Cuts at: 2532 3050 3082 3177
Size: 518 32 95
Fragments arranged by size:
888 518 350 281 244 225 174 95
59 55 32 32 32 32 32 32
32 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 1447 3177
Size: 1447 1730
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 93 1320 1488 1509 2056 2247 3177
Size: 93 1227 168 21 547 191 930
Fragments arranged by size:
1227 930 547 191 168 93 21
XmaI C'CCGG_G
Cuts at: 0 630 1505 2292 3131 3177
Size: 630 875 787 839 46
Fragments arranged by size:
875 839 787 630 46
ZraI GAC'GTC
Cuts at: 0 2613 3177
Size: 2613 564
Enzymes that do cut:
AarI AatII AccI AcuI AhdI AleI AlfI AloI
AlwNI ApaI AvaI AvrII BamHI BanI BanII BbeI
BbvCI BglI BlpI Bme1580I BmgBI BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstEII BstXI
BstYI BtgI BtsI ClaI CspCI DraIII DrdI EaeI
EagI EcoICRI Eco57MI EcoNI EcoO109I FalI FseI FspI
HaeII Hin4I HincII KasI MfeI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PasI PflMI
PfoI PmlI PpuMI PspOMI PstI PvuII SacI SalI
SanDI SbfI SfcI SfoI SmaI SmlI SphI StuI
StyI TaqII TatI TstI Tth111I XcmI XmaI ZraI
Enzymes that do not cut:
Acc65I AclI AfeI AflII AflIII AgeI ApaLI ApoI
AscI AseI AsiSI BaeI BbsI BcgI BciVI BclI
BfrBI BglII BmtI BsaBI BsiWI BsmI BsmBI BspHI
BstBI BstZ17I Bsu36I BtgZI DraI EarI EciI EcoRI
EcoRV FspAI HindIII HpaI KpnI MluI NdeI NheI
NotI NruI NsiI PacI PciI PmeI PpiI PshAI
PsiI PspXI PsrI PvuI RsrII SacII SapI ScaI
SexAI SfiI SgfI SgrAI SnaBI SpeI SrfI SspI
SwaI XbaI XhoI XmnI