Restriction map for
pF1KB7307
(Linear) (Six-base) MAPSORT of: KIBB7307.seq Check: 5576 from: 1 to: 5118
KIBB7307.orf
With 182 enzymes: SgfI *
April 24, 2009 13:07 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1111 5118
Size: 1111 4007
AccI GT'mk_AC
Cuts at: 0 1916 5118
Size: 1916 3202
Acc65I G'GTAC_C
Cuts at: 0 2895 5118
Size: 2895 2223
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 460 1035 1280 4553 5118
Size: 460 575 245 3273 565
Fragments arranged by size:
3273 575 565 460 245
AflII C'TTAA_G
Cuts at: 0 669 5118
Size: 669 4449
AflIII A'CryG_T
Cuts at: 0 1738 5118
Size: 1738 3380
AhdI GACnn_n'nnGTC
Cuts at: 0 3112 5118
Size: 3112 2006
AleI CACnn'nnGTG
Cuts at: 0 1052 5118
Size: 1052 4066
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 4578 4610 5118
Size: 4578 32 508
Fragments arranged by size:
4578 508 32
AlwNI CAG_nnn'CTG
Cuts at: 0 409 599 4330 4723 5118
Size: 409 190 3731 393 395
Fragments arranged by size:
3731 409 395 393 190
ApaLI G'TGCA_C
Cuts at: 0 4792 5118
Size: 4792 326
ApoI r'AATT_y
Cuts at: 0 82 161 412 461 547 1182 1569 2002
Size: 82 79 251 49 86 635 387 433
Cuts at: 2002 2327 2872 3149 3304 3439 3694 3874 4082
Size: 325 545 277 155 135 255 180 208
Cuts at: 4082 4343 4530 5118
Size: 261 187 588
Fragments arranged by size:
635 588 545 433 387 325 277 261
255 251 208 187 180 155 135 86
82 79 49
AseI AT'TA_AT
Cuts at: 0 905 974 5118
Size: 905 69 4144
Fragments arranged by size:
4144 905 69
AvaI C'yCGr_G
Cuts at: 0 269 5118
Size: 269 4849
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 3656 3689 5118
Size: 3656 33 1429
Fragments arranged by size:
3656 1429 33
BanI G'GyrC_C
Cuts at: 0 986 2895 3370 4713 5118
Size: 986 1909 475 1343 405
Fragments arranged by size:
1909 1343 986 475 405
BanII G_rGCy'C
Cuts at: 0 2982 3633 4519 4948 5118
Size: 2982 651 886 429 170
Fragments arranged by size:
2982 886 651 429 170
BbsI GAAGACnn'nnnn_
Cuts at: 0 332 1616 5118
Size: 332 1284 3502
Fragments arranged by size:
3502 1284 332
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2029 2063 5118
Size: 2029 34 3055
Fragments arranged by size:
3055 2029 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 50 2767 4326 5118
Size: 50 2717 1559 792
Fragments arranged by size:
2717 1559 792 50
BclI T'GATC_A
Cuts at: 0 1359 5118
Size: 1359 3759
BfrBI ATG'CAT
Cuts at: 0 4645 5118
Size: 4645 473
BglI GCCn_nnn'nGGC
Cuts at: 0 3368 5118
Size: 3368 1750
Bme1580I G_kGCm'C
Cuts at: 0 989 4796 5118
Size: 989 3807 322
Fragments arranged by size:
3807 989 322
BmrI ACTGGGnnnn_n'
Cuts at: 0 2316 5118
Size: 2316 2802
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2849 5118
Size: 2849 2269
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 223 542 704 1449 2210 2630 2966 3071
Size: 223 319 162 745 761 420 336 105
Cuts at: 3071 3467 4580 4871 5118
Size: 396 1113 291 247
Fragments arranged by size:
1113 761 745 420 396 336 319 291
247 223 162 105
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2185 3582 3653 3767 4862 5000 5118
Size: 2185 1397 71 114 1095 138 118
Fragments arranged by size:
2185 1397 1095 138 118 114 71
BsaI GGTCTCn'nnnn_
Cuts at: 0 2834 5118
Size: 2834 2284
BsaBI GATnn'nnATC
Cuts at: 0 581 1037 5118
Size: 581 456 4081
Fragments arranged by size:
4081 581 456
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 196 226 4578 4608 5118
Size: 196 30 4352 30 510
Fragments arranged by size:
4352 510 196 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 257 2905 5118
Size: 257 2648 2213
Fragments arranged by size:
2648 2213 257
BseYI C'CCAG_C
Cuts at: 0 2583 4297 4619 4771 5118
Size: 2583 1714 322 152 347
Fragments arranged by size:
2583 1714 347 322 152
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2589 3791 4475 5118
Size: 2589 1202 684 643
Fragments arranged by size:
2589 1202 684 643
BsiHKAI G_wGCw'C
Cuts at: 0 130 720 1049 2982 4796 4948 5118
Size: 130 590 329 1933 1814 152 170
Fragments arranged by size:
1933 1814 590 329 170 152 130
BsmI GAATG_Cn'
Cuts at: 0 2669 4367 5118
Size: 2669 1698 751
Fragments arranged by size:
2669 1698 751
BsmBI CGTCTCn'nnnn_
Cuts at: 0 4682 5118
Size: 4682 436
Bsp1286I G_dGCh'C
Cuts at: 0 130 720 989 1049 2982 3633 4519 4796
Size: 130 590 269 60 1933 651 886 277
Cuts at: 4796 4948 5118
Size: 152 170
Fragments arranged by size:
1933 886 651 590 277 269 170 152
130 60
BspHI T'CATG_A
Cuts at: 0 579 648 909 5118
Size: 579 69 261 4209
Fragments arranged by size:
4209 579 261 69
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1111 4806 4992 5118
Size: 1111 3695 186 126
Fragments arranged by size:
3695 1111 186 126
BsrBI CCG'CTC
Cuts at: 0 36 5118
Size: 36 5082
BsrGI T'GTAC_A
Cuts at: 0 1876 3582 5118
Size: 1876 1706 1536
Fragments arranged by size:
1876 1706 1536
BstAPI GCAn_nnn'nTGC
Cuts at: 0 4498 5118
Size: 4498 620
BstXI CCAn_nnnn'nTGG
Cuts at: 0 292 4767 5118
Size: 292 4475 351
Fragments arranged by size:
4475 351 292
BstYI r'GATC_y
Cuts at: 0 26 556 2818 3724 4304 5118
Size: 26 530 2262 906 580 814
Fragments arranged by size:
2262 906 814 580 530 26
Bsu36I CC'TnA_GG
Cuts at: 0 4521 5118
Size: 4521 597
BtgI C'CryG_G
Cuts at: 0 1730 1840 4058 4699 4766 4936 5118
Size: 1730 110 2218 641 67 170 182
Fragments arranged by size:
2218 1730 641 182 170 110 67
BtsI GCAGTG_nn'
Cuts at: 0 916 5118
Size: 916 4202
DraI TTT'AAA
Cuts at: 0 813 1647 2864 3879 5118
Size: 813 834 1217 1015 1239
Fragments arranged by size:
1239 1217 1015 834 813
EaeI y'GGCC_r
Cuts at: 0 419 1843 2750 5118
Size: 419 1424 907 2368
Fragments arranged by size:
2368 1424 907 419
EarI CTCTTCn'nnn_
Cuts at: 0 368 929 1757 2808 3878 4025 4164 5061
Size: 368 561 828 1051 1070 147 139 897
Cuts at: 5061 5118
Size: 57
Fragments arranged by size:
1070 1051 897 828 561 368 147 139
57
EcoICRI GAG'CTC
Cuts at: 0 2980 4946 5118
Size: 2980 1966 172
Fragments arranged by size:
2980 1966 172
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 223 460 542 704 1035 1280 1449 2210
Size: 223 237 82 162 331 245 169 761
Cuts at: 2210 2630 2966 3071 3467 4553 4580 4871 5118
Size: 420 336 105 396 1086 27 291 247
Fragments arranged by size:
1086 761 420 396 336 331 291 247
245 237 223 169 162 105 82 27
EcoNI CCTnn'n_nnAGG
Cuts at: 0 524 1756 3476 4386 5118
Size: 524 1232 1720 910 732
Fragments arranged by size:
1720 1232 910 732 524
EcoRI G'AATT_C
Cuts at: 0 3694 5118
Size: 3694 1424
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 2229 4510 5118
Size: 2229 2281 608
Fragments arranged by size:
2281 2229 608
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 479 511 1324 1356 2074 2106 3098 3130
Size: 479 32 813 32 718 32 992 32
Cuts at: 3130 3359 3391 3898 3930 4024 4056 4933 4965
Size: 229 32 507 32 94 32 877 32
Cuts at: 4965 5118
Size: 153
Fragments arranged by size:
992 877 813 718 507 479 229 153
94 32 32 32 32 32 32 32
32
HincII GTy'rAC
Cuts at: 0 1332 3455 3554 5045 5118
Size: 1332 2123 99 1491 73
Fragments arranged by size:
2123 1491 1332 99 73
HindIII A'AGCT_T
Cuts at: 0 2239 5118
Size: 2239 2879
HpaI GTT'AAC
Cuts at: 0 3455 5118
Size: 3455 1663
KpnI G_GTAC'C
Cuts at: 0 2899 5118
Size: 2899 2219
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 413 1174 2048 2898 4338 4940 5118
Size: 413 761 874 850 1440 602 178
Fragments arranged by size:
1440 874 850 761 602 413 178
MscI TGG'CCA
Cuts at: 0 421 1845 2752 5118
Size: 421 1424 907 2366
Fragments arranged by size:
2366 1424 907 421
MslI CAynn'nnrTG
Cuts at: 0 137 1052 4121 4765 5118
Size: 137 915 3069 644 353
Fragments arranged by size:
3069 915 644 353 137
MspA1I CmG'CkG
Cuts at: 0 3580 4573 5118
Size: 3580 993 545
Fragments arranged by size:
3580 993 545
NcoI C'CATG_G
Cuts at: 0 1730 1840 4058 4699 4766 5118
Size: 1730 110 2218 641 67 352
Fragments arranged by size:
2218 1730 641 352 110 67
NdeI CA'TA_TG
Cuts at: 0 939 5118
Size: 939 4179
NsiI A_TGCA'T
Cuts at: 0 4647 5118
Size: 4647 471
NspI r_CATG'y
Cuts at: 0 1373 1586 1742 5118
Size: 1373 213 156 3376
Fragments arranged by size:
3376 1373 213 156
PciI A'CATG_T
Cuts at: 0 1738 5118
Size: 1738 3380
PflMI CCAn_nnn'nTGG
Cuts at: 0 961 4615 4723 5118
Size: 961 3654 108 395
Fragments arranged by size:
3654 961 395 108
PfoI T'CCnGG_A
Cuts at: 0 519 1426 2226 3444 5118
Size: 519 907 800 1218 1674
Fragments arranged by size:
1674 1218 907 800 519
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 665 697 3010 3042 4578 4610 5118
Size: 665 32 2313 32 1536 32 508
Fragments arranged by size:
2313 1536 665 508 32 32 32
PspXI vC'TCGA_Gb
Cuts at: 0 269 5118
Size: 269 4849
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 927 959 5118
Size: 927 32 4159
Fragments arranged by size:
4159 927 32
PstI C_TGCA'G
Cuts at: 0 2382 2946 4817 5118
Size: 2382 564 1871 301
Fragments arranged by size:
2382 1871 564 301
PvuII CAG'CTG
Cuts at: 0 3580 4573 5118
Size: 3580 993 545
Fragments arranged by size:
3580 993 545
SacI G_AGCT'C
Cuts at: 0 2982 4948 5118
Size: 2982 1966 170
Fragments arranged by size:
2982 1966 170
SapI GCTCTTCn'nnn_
Cuts at: 0 4025 5118
Size: 4025 1093
ScaI AGT'ACT
Cuts at: 0 2968 3120 5118
Size: 2968 152 1998
Fragments arranged by size:
2968 1998 152
SexAI A'CCwGG_T
Cuts at: 0 3471 5118
Size: 3471 1647
SfcI C'TryA_G
Cuts at: 0 251 368 2378 2942 4813 5118
Size: 251 117 2010 564 1871 305
Fragments arranged by size:
2010 1871 564 305 251 117
SmlI C'TyrA_G
Cuts at: 0 269 669 2164 3597 3668 3782 4877 5015
Size: 269 400 1495 1433 71 114 1095 138
Cuts at: 5015 5118
Size: 103
Fragments arranged by size:
1495 1433 1095 400 269 138 114 103
71
SphI G_CATG'C
Cuts at: 0 1586 5118
Size: 1586 3532
SspI AAT'ATT
Cuts at: 0 1630 2719 5118
Size: 1630 1089 2399
Fragments arranged by size:
2399 1630 1089
StyI C'CwwG_G
Cuts at: 0 1730 1840 2760 3503 4058 4699 4766 5118
Size: 1730 110 920 743 555 641 67 352
Fragments arranged by size:
1730 920 743 641 555 352 110 67
SwaI ATTT'AAAT
Cuts at: 0 813 5118
Size: 813 4305
TatI w'GTAC_w
Cuts at: 0 1486 1548 1876 2319 2729 2966 3118 3411
Size: 1486 62 328 443 410 237 152 293
Cuts at: 3411 3582 3996 5118
Size: 171 414 1122
Fragments arranged by size:
1486 1122 443 414 410 328 293 237
171 152 62
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1481 1513 4267 4299 4697 4729 5118
Size: 1481 32 2754 32 398 32 389
Fragments arranged by size:
2754 1481 398 389 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 599 5118
Size: 599 4519
XbaI T'CTAG_A
Cuts at: 0 2445 5118
Size: 2445 2673
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 517 4616 5118
Size: 517 4099 502
Fragments arranged by size:
4099 517 502
XhoI C'TCGA_G
Cuts at: 0 269 5118
Size: 269 4849
XmnI GAAnn'nnTTC
Cuts at: 0 238 362 680 1538 2954 3077 3440 4347
Size: 238 124 318 858 1416 123 363 907
Cuts at: 4347 4732 5118
Size: 385 386
Fragments arranged by size:
1416 907 858 386 385 363 318 238
124 123
Enzymes that do cut:
AarI AccI Acc65I AcuI AflII AflIII AhdI AleI
AloI AlwNI ApaLI ApoI AseI AvaI BaeI BanI
BanII BbsI BcgI BciVI BclI BfrBI BglI Bme1580I
BmrI BplI BpmI BpuEI BsaI BsaBI BsaXI BseRI
BseYI BsgI BsiHKAI BsmI BsmBI Bsp1286I BspHI BspMI
BsrBI BsrGI BstAPI BstXI BstYI Bsu36I BtgI BtsI
DraI EaeI EarI EcoICRI Eco57MI EcoNI EcoRI FalI
Hin4I HincII HindIII HpaI KpnI MmeI MscI MslI
MspA1I NcoI NdeI NsiI NspI PciI PflMI PfoI
PpiI PspXI PsrI PstI PvuII SacI SapI ScaI
SexAI SfcI SmlI SphI SspI StyI SwaI TatI
TstI Tth111I XbaI XcmI XhoI XmnI
Enzymes that do not cut:
AatII AclI AfeI AgeI AlfI ApaI AscI AsiSI
AvrII BamHI BbeI BbvCI BglII BlpI BmgBI BmtI
Bpu10I BsaAI BsaHI BsaWI BsiEI BsiWI BspEI BsrDI
BsrFI BssHII BssSI BstBI BstEII BstZ17I BtgZI ClaI
CspCI DraIII DrdI EagI EciI EcoO109I EcoRV FseI
FspI FspAI HaeII KasI MfeI MluI NaeI NarI
NgoMIV NheI NotI NruI PacI PasI PmeI PmlI
PpuMI PshAI PsiI PspOMI PvuI RsrII SacII SalI
SanDI SbfI SfiI SfoI SgfI SgrAI SmaI SnaBI
SpeI SrfI StuI TaqII XmaI ZraI