Restriction map for
pF1KB7306
(Linear) (Six-base) MAPSORT of: KIBB7306.seq Check: 1887 from: 1 to: 3192
KIBB7306.orf
With 182 enzymes: SgfI *
March 3, 2008 11:00 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1204 1414 1804 3192
Size: 1204 210 390 1388
Fragments arranged by size:
1388 1204 390 210
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 869 1061 3192
Size: 869 192 2131
Fragments arranged by size:
2131 869 192
AflIII A'CryG_T
Cuts at: 0 2317 2849 3192
Size: 2317 532 343
Fragments arranged by size:
2317 532 343
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1113 3192
Size: 1113 2079
AlwNI CAG_nnn'CTG
Cuts at: 0 1695 3192
Size: 1695 1497
ApaI G_GGCC'C
Cuts at: 0 1171 1381 3192
Size: 1171 210 1811
Fragments arranged by size:
1811 1171 210
ApaLI G'TGCA_C
Cuts at: 0 389 2809 3192
Size: 389 2420 383
Fragments arranged by size:
2420 389 383
ApoI r'AATT_y
Cuts at: 0 94 145 644 788 834 1492 1900 2551
Size: 94 51 499 144 46 658 408 651
Cuts at: 2551 2752 3192
Size: 201 440
Fragments arranged by size:
658 651 499 440 408 201 144 94
51 46
AvaI C'yCGr_G
Cuts at: 0 2165 2366 3192
Size: 2165 201 826
Fragments arranged by size:
2165 826 201
BanII G_rGCy'C
Cuts at: 0 258 1171 1381 3192
Size: 258 913 210 1811
Fragments arranged by size:
1811 913 258 210
BbsI GAAGACnn'nnnn_
Cuts at: 0 519 568 1206 2004 2157 2486 2942 3192
Size: 519 49 638 798 153 329 456 250
Fragments arranged by size:
798 638 519 456 329 250 153 49
BciVI GTATCCnnnnn_n'
Cuts at: 0 2307 3192
Size: 2307 885
BfrBI ATG'CAT
Cuts at: 0 759 975 1395 1572 3192
Size: 759 216 420 177 1620
Fragments arranged by size:
1620 759 420 216 177
BglI GCCn_nnn'nGGC
Cuts at: 0 749 1562 1970 2621 3192
Size: 749 813 408 651 571
Fragments arranged by size:
813 749 651 571 408
BlpI GC'TnA_GC
Cuts at: 0 593 3192
Size: 593 2599
Bme1580I G_kGCm'C
Cuts at: 0 393 777 1171 1381 2813 3192
Size: 393 384 394 210 1432 379
Fragments arranged by size:
1432 394 393 384 379 210
BmrI ACTGGGnnnn_n'
Cuts at: 0 2800 3192
Size: 2800 392
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2262 3192
Size: 2262 930
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2912 3192
Size: 2912 280
BsaAI yAC'GTr
Cuts at: 0 194 2185 3192
Size: 194 1991 1007
Fragments arranged by size:
1991 1007 194
BsaBI GATnn'nnATC
Cuts at: 0 2301 2632 3192
Size: 2301 331 560
Fragments arranged by size:
2301 560 331
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1183 1213 1393 1423 1783 1813 3192
Size: 1183 30 180 30 360 30 1379
Fragments arranged by size:
1379 1183 360 180 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1315 2111 3154 3192
Size: 1315 796 1043 38
Fragments arranged by size:
1315 1043 796 38
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1719 1920 2346 2571 2766 2874 3192
Size: 1719 201 426 225 195 108 318
Fragments arranged by size:
1719 426 318 225 201 195 108
BsiHKAI G_wGCw'C
Cuts at: 0 258 393 2813 3192
Size: 258 135 2420 379
Fragments arranged by size:
2420 379 258 135
BsmI GAATG_Cn'
Cuts at: 0 411 3192
Size: 411 2781
Bsp1286I G_dGCh'C
Cuts at: 0 258 393 777 1171 1381 2813 3192
Size: 258 135 384 394 210 1432 379
Fragments arranged by size:
1432 394 384 379 258 210 135
BspHI T'CATG_A
Cuts at: 0 3084 3192
Size: 3084 108
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1204 1414 1804 3192
Size: 1204 210 390 1388
Fragments arranged by size:
1388 1204 390 210
BsrDI GCAATG_nn'
Cuts at: 0 590 2532 3192
Size: 590 1942 660
Fragments arranged by size:
1942 660 590
BsrGI T'GTAC_A
Cuts at: 0 715 3192
Size: 715 2477
BssSI C'ACGA_G
Cuts at: 0 1046 3192
Size: 1046 2146
BstAPI GCAn_nnn'nTGC
Cuts at: 0 397 589 1196 1406 1796 3192
Size: 397 192 607 210 390 1396
Fragments arranged by size:
1396 607 397 390 210 192
BstBI TT'CG_AA
Cuts at: 0 2556 2757 3192
Size: 2556 201 435
Fragments arranged by size:
2556 435 201
BstYI r'GATC_y
Cuts at: 0 2538 3192
Size: 2538 654
BtgI C'CryG_G
Cuts at: 0 738 1551 1785 1980 3192
Size: 738 813 234 195 1212
Fragments arranged by size:
1212 813 738 234 195
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1910 3192
Size: 1910 1282
BtsI GCAGTG_nn'
Cuts at: 0 489 1107 1494 1707 2754 3192
Size: 489 618 387 213 1047 438
Fragments arranged by size:
1047 618 489 438 387 213
DraI TTT'AAA
Cuts at: 0 300 605 3029 3192
Size: 300 305 2424 163
Fragments arranged by size:
2424 305 300 163
DraIII CAC_nnn'GTG
Cuts at: 0 397 3192
Size: 397 2795
EaeI y'GGCC_r
Cuts at: 0 3051 3192
Size: 3051 141
EarI CTCTTCn'nnn_
Cuts at: 0 226 1616 2042 2696 3192
Size: 226 1390 426 654 496
Fragments arranged by size:
1390 654 496 426 226
EcoICRI GAG'CTC
Cuts at: 0 256 3192
Size: 256 2936
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 869 1061 2262 3192
Size: 869 192 1201 930
Fragments arranged by size:
1201 930 869 192
EcoNI CCTnn'n_nnAGG
Cuts at: 0 2609 3192
Size: 2609 583
EcoO109I rG'GnC_Cy
Cuts at: 0 419 1167 1377 3192
Size: 419 748 210 1815
Fragments arranged by size:
1815 748 419 210
EcoRI G'AATT_C
Cuts at: 0 644 3192
Size: 644 2548
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 2028 3192
Size: 2028 1164
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 447 479 1850 1882 2185 2217 2994 3026
Size: 447 32 1371 32 303 32 777 32
Cuts at: 3026 3192
Size: 166
Fragments arranged by size:
1371 777 447 303 166 32 32 32
32
HindIII A'AGCT_T
Cuts at: 0 1114 2578 2876 2893 3192
Size: 1114 1464 298 17 299
Fragments arranged by size:
1464 1114 299 298 17
MscI TGG'CCA
Cuts at: 0 3053 3192
Size: 3053 139
MslI CAynn'nnrTG
Cuts at: 0 1524 3192
Size: 1524 1668
MspA1I CmG'CkG
Cuts at: 0 2159 2455 3192
Size: 2159 296 737
Fragments arranged by size:
2159 737 296
NcoI C'CATG_G
Cuts at: 0 1980 3192
Size: 1980 1212
NdeI CA'TA_TG
Cuts at: 0 375 1086 2619 3192
Size: 375 711 1533 573
Fragments arranged by size:
1533 711 573 375
NsiI A_TGCA'T
Cuts at: 0 761 977 1397 1574 3192
Size: 761 216 420 177 1618
Fragments arranged by size:
1618 761 420 216 177
NspI r_CATG'y
Cuts at: 0 1895 2321 2565 2853 3078 3177 3192
Size: 1895 426 244 288 225 99 15
Fragments arranged by size:
1895 426 288 244 225 99 15
PciI A'CATG_T
Cuts at: 0 2317 2849 3192
Size: 2317 532 343
Fragments arranged by size:
2317 532 343
PflMI CCAn_nnn'nTGG
Cuts at: 0 739 3192
Size: 739 2453
PfoI T'CCnGG_A
Cuts at: 0 1327 3192
Size: 1327 1865
PmlI CAC'GTG
Cuts at: 0 194 3192
Size: 194 2998
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1670 1702 3192
Size: 1670 32 1490
Fragments arranged by size:
1670 1490 32
PpuMI rG'GwC_Cy
Cuts at: 0 419 3192
Size: 419 2773
PspOMI G'GGCC_C
Cuts at: 0 1167 1377 3192
Size: 1167 210 1815
Fragments arranged by size:
1815 1167 210
PstI C_TGCA'G
Cuts at: 0 892 1489 2073 3192
Size: 892 597 584 1119
Fragments arranged by size:
1119 892 597 584
PvuII CAG'CTG
Cuts at: 0 2159 2455 3192
Size: 2159 296 737
Fragments arranged by size:
2159 737 296
SacI G_AGCT'C
Cuts at: 0 258 3192
Size: 258 2934
SanDI GG'GwC_CC
Cuts at: 0 419 3192
Size: 419 2773
SfcI C'TryA_G
Cuts at: 0 888 1485 2069 3192
Size: 888 597 584 1123
Fragments arranged by size:
1123 888 597 584
SmlI C'TyrA_G
Cuts at: 0 2366 2927 3192
Size: 2366 561 265
Fragments arranged by size:
2366 561 265
SphI G_CATG'C
Cuts at: 0 2565 3078 3177 3192
Size: 2565 513 99 15
Fragments arranged by size:
2565 513 99 15
StuI AGG'CCT
Cuts at: 0 1423 3192
Size: 1423 1769
StyI C'CwwG_G
Cuts at: 0 32 260 1980 3192
Size: 32 228 1720 1212
Fragments arranged by size:
1720 1212 228 32
TatI w'GTAC_w
Cuts at: 0 715 1285 2161 2419 3124 3192
Size: 715 570 876 258 705 68
Fragments arranged by size:
876 715 705 570 258 68
Tth111I GACn'n_nGTC
Cuts at: 0 2380 3192
Size: 2380 812
XbaI T'CTAG_A
Cuts at: 0 1770 3192
Size: 1770 1422
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1171 1381 1510 2344 3192
Size: 1171 210 129 834 848
Fragments arranged by size:
1171 848 834 210 129
XhoI C'TCGA_G
Cuts at: 0 2366 3192
Size: 2366 826
XmnI GAAnn'nnTTC
Cuts at: 0 1685 3192
Size: 1685 1507
Enzymes that do cut:
AarI AcuI AflIII AlfI AlwNI ApaI ApaLI ApoI
AvaI BanII BbsI BciVI BfrBI BglI BlpI Bme1580I
BmrI BpmI BpuEI BsaAI BsaBI BsaXI BseRI BsgI
BsiHKAI BsmI Bsp1286I BspHI BspMI BsrDI BsrGI BssSI
BstAPI BstBI BstYI BtgI BtgZI BtsI DraI DraIII
EaeI EarI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
Hin4I HindIII MscI MslI MspA1I NcoI NdeI NsiI
NspI PciI PflMI PfoI PmlI PpiI PpuMI PspOMI
PstI PvuII SacI SanDI SfcI SmlI SphI StuI
StyI TatI Tth111I XbaI XcmI XhoI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AgeI AhdI
AleI AloI AscI AseI AsiSI AvrII BaeI BamHI
BanI BbeI BbvCI BcgI BclI BglII BmgBI BmtI
BplI Bpu10I BsaI BsaHI BsaWI BseYI BsiEI BsiWI
BsmBI BspEI BsrBI BsrFI BssHII BstEII BstXI BstZ17I
Bsu36I ClaI CspCI DrdI EagI EciI EcoRV FseI
FspI FspAI HaeII HincII HpaI KasI KpnI MfeI
MluI MmeI NaeI NarI NgoMIV NheI NotI NruI
PacI PasI PmeI PshAI PsiI PspXI PsrI PvuI
RsrII SacII SalI SapI SbfI ScaI SexAI SfiI
SfoI SgfI SgrAI SmaI SnaBI SpeI SrfI SspI
SwaI TaqII TstI XmaI ZraI