Restriction map for
pF1KB7305
(Linear) (Six-base) MAPSORT of: KIBB7305.seq Check: 7925 from: 1 to: 2430
KIBB7305.orf
With 182 enzymes: SgfI *
March 2, 2010 14:27 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1063 1504 2430
Size: 1063 441 926
Fragments arranged by size:
1063 926 441
AhdI GACnn_n'nnGTC
Cuts at: 0 1591 2430
Size: 1591 839
ApaLI G'TGCA_C
Cuts at: 0 533 2430
Size: 533 1897
AseI AT'TA_AT
Cuts at: 0 808 2430
Size: 808 1622
AvaI C'yCGr_G
Cuts at: 0 2397 2430
Size: 2397 33
BanI G'GyrC_C
Cuts at: 0 2123 2430
Size: 2123 307
BanII G_rGCy'C
Cuts at: 0 85 914 2120 2430
Size: 85 829 1206 310
Fragments arranged by size:
1206 829 310 85
BbsI GAAGACnn'nnnn_
Cuts at: 0 2348 2430
Size: 2348 82
BciVI GTATCCnnnnn_n'
Cuts at: 0 482 2430
Size: 482 1948
BfrBI ATG'CAT
Cuts at: 0 958 2430
Size: 958 1472
BglI GCCn_nnn'nGGC
Cuts at: 0 2132 2141 2430
Size: 2132 9 289
Fragments arranged by size:
2132 289 9
BglII A'GATC_T
Cuts at: 0 310 2430
Size: 310 2120
BlpI GC'TnA_GC
Cuts at: 0 79 2006 2430
Size: 79 1927 424
Fragments arranged by size:
1927 424 79
Bme1580I G_kGCm'C
Cuts at: 0 537 2430
Size: 537 1893
BmrI ACTGGGnnnn_n'
Cuts at: 0 1649 2430
Size: 1649 781
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 104 2430
Size: 104 2326
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2374 2430
Size: 2374 56
Bpu10I CC'TnA_GC
Cuts at: 0 2119 2430
Size: 2119 311
BsaI GGTCTCn'nnnn_
Cuts at: 0 2350 2430
Size: 2350 80
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1841 1864 2430
Size: 1841 23 566
Fragments arranged by size:
1841 566 23
BsiHKAI G_wGCw'C
Cuts at: 0 85 537 914 2120 2430
Size: 85 452 377 1206 310
Fragments arranged by size:
1206 452 377 310 85
BsmI GAATG_Cn'
Cuts at: 0 1924 2430
Size: 1924 506
Bsp1286I G_dGCh'C
Cuts at: 0 85 537 914 2120 2430
Size: 85 452 377 1206 310
Fragments arranged by size:
1206 452 377 310 85
BspHI T'CATG_A
Cuts at: 0 2112 2430
Size: 2112 318
BsrBI CCG'CTC
Cuts at: 0 2391 2430
Size: 2391 39
BsrDI GCAATG_nn'
Cuts at: 0 614 1532 2000 2430
Size: 614 918 468 430
Fragments arranged by size:
918 614 468 430
BsrGI T'GTAC_A
Cuts at: 0 918 2430
Size: 918 1512
BstBI TT'CG_AA
Cuts at: 0 1803 2430
Size: 1803 627
BstEII G'GTnAC_C
Cuts at: 0 3 2430
Size: 3 2427
BstXI CCAn_nnnn'nTGG
Cuts at: 0 144 2077 2430
Size: 144 1933 353
Fragments arranged by size:
1933 353 144
BstYI r'GATC_y
Cuts at: 0 310 2430
Size: 310 2120
BtsI GCAGTG_nn'
Cuts at: 0 537 2318 2430
Size: 537 1781 112
Fragments arranged by size:
1781 537 112
DraI TTT'AAA
Cuts at: 0 840 1316 2430
Size: 840 476 1114
Fragments arranged by size:
1114 840 476
EaeI y'GGCC_r
Cuts at: 0 134 2430
Size: 134 2296
EarI CTCTTCn'nnn_
Cuts at: 0 348 1265 1471 1800 2400 2430
Size: 348 917 206 329 600 30
Fragments arranged by size:
917 600 348 329 206 30
EcoICRI GAG'CTC
Cuts at: 0 83 912 2118 2430
Size: 83 829 1206 312
Fragments arranged by size:
1206 829 312 83
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1063 1504 2374 2430
Size: 1063 441 870 56
Fragments arranged by size:
1063 870 441 56
EcoNI CCTnn'n_nnAGG
Cuts at: 0 667 2430
Size: 667 1763
EcoRV GAT'ATC
Cuts at: 0 1026 2430
Size: 1026 1404
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 679 1891 2053 2065 2430
Size: 679 1212 162 12 365
Fragments arranged by size:
1212 679 365 162 12
HaeII r_GCGC'y
Cuts at: 0 1623 2430
Size: 1623 807
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 123 155 286 318 480 512 1577 1609
Size: 123 32 131 32 162 32 1065 32
Cuts at: 1609 2191 2223 2430
Size: 582 32 207
Fragments arranged by size:
1065 582 207 162 131 123 32 32
32 32 32
HincII GTy'rAC
Cuts at: 0 2184 2430
Size: 2184 246
HindIII A'AGCT_T
Cuts at: 0 785 1228 2035 2430
Size: 785 443 807 395
Fragments arranged by size:
807 785 443 395
MfeI C'AATT_G
Cuts at: 0 1004 2430
Size: 1004 1426
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 732 1382 2430
Size: 732 650 1048
Fragments arranged by size:
1048 732 650
MscI TGG'CCA
Cuts at: 0 136 2430
Size: 136 2294
MslI CAynn'nnrTG
Cuts at: 0 1537 2430
Size: 1537 893
NsiI A_TGCA'T
Cuts at: 0 960 2430
Size: 960 1470
SacI G_AGCT'C
Cuts at: 0 85 914 2120 2430
Size: 85 829 1206 310
Fragments arranged by size:
1206 829 310 85
SapI GCTCTTCn'nnn_
Cuts at: 0 348 1265 1800 2430
Size: 348 917 535 630
Fragments arranged by size:
917 630 535 348
SfcI C'TryA_G
Cuts at: 0 1381 1831 2430
Size: 1381 450 599
Fragments arranged by size:
1381 599 450
SspI AAT'ATT
Cuts at: 0 1509 2430
Size: 1509 921
StyI C'CwwG_G
Cuts at: 0 663 2430
Size: 663 1767
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 87 1674 2430
Size: 87 1587 756
Fragments arranged by size:
1587 756 87
TatI w'GTAC_w
Cuts at: 0 918 2430
Size: 918 1512
XmnI GAAnn'nnTTC
Cuts at: 0 476 2430
Size: 476 1954
Enzymes that do cut:
AcuI AhdI ApaLI AseI AvaI BanI BanII BbsI
BciVI BfrBI BglI BglII BlpI Bme1580I BmrI BplI
BpmI Bpu10I BsaI BsgI BsiHKAI BsmI Bsp1286I BspHI
BsrBI BsrDI BsrGI BstBI BstEII BstXI BstYI BtsI
DraI EaeI EarI EcoICRI Eco57MI EcoNI EcoRV FalI
HaeII Hin4I HincII HindIII MfeI MmeI MscI MslI
NsiI SacI SapI SfcI SspI StyI TaqII TatI
XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AleI AlfI AloI AlwNI ApaI ApoI AscI
AsiSI AvrII BaeI BamHI BbeI BbvCI BcgI BclI
BmgBI BmtI BpuEI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsiEI BsiWI BsmBI BspEI BspMI BsrFI
BssHII BssSI BstAPI BstZ17I Bsu36I BtgI BtgZI ClaI
CspCI DraIII DrdI EagI EciI EcoO109I EcoRI FseI
FspI FspAI HpaI KasI KpnI MluI MspA1I NaeI
NarI NcoI NdeI NgoMIV NheI NotI NruI NspI
PacI PasI PciI PflMI PfoI PmeI PmlI PpiI
PpuMI PshAI PsiI PspOMI PspXI PsrI PstI PvuI
PvuII RsrII SacII SalI SanDI SbfI ScaI SexAI
SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SrfI StuI SwaI TstI Tth111I XbaI XcmI
XhoI XmaI ZraI