Restriction map for
pF1KB7300
(Linear) (Six-base) MAPSORT of: KIBB7300.seq Check: 2465 from: 1 to: 2868
KIBB7300.orf
With 182 enzymes: SgfI *
March 3, 2008 11:00 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 2421 2753 2868
Size: 2421 332 115
Fragments arranged by size:
2421 332 115
AccI GT'mk_AC
Cuts at: 0 1832 2868
Size: 1832 1036
Acc65I G'GTAC_C
Cuts at: 0 1510 2740 2868
Size: 1510 1230 128
Fragments arranged by size:
1510 1230 128
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 143 550 1976 2235 2868
Size: 143 407 1426 259 633
Fragments arranged by size:
1426 633 407 259 143
AflIII A'CryG_T
Cuts at: 0 803 2868
Size: 803 2065
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 278 1098 2553 2868
Size: 278 820 1455 315
Fragments arranged by size:
1455 820 315 278
AlwNI CAG_nnn'CTG
Cuts at: 0 557 1584 2868
Size: 557 1027 1284
Fragments arranged by size:
1284 1027 557
ApaI G_GGCC'C
Cuts at: 0 2034 2465 2868
Size: 2034 431 403
Fragments arranged by size:
2034 431 403
AseI AT'TA_AT
Cuts at: 0 901 2868
Size: 901 1967
AvaI C'yCGr_G
Cuts at: 0 513 2464 2868
Size: 513 1951 404
Fragments arranged by size:
1951 513 404
BanI G'GyrC_C
Cuts at: 0 16 925 1133 1510 1770 1822 2740 2859
Size: 16 909 208 377 260 52 918 119
Cuts at: 2859 2868
Size: 9
Fragments arranged by size:
918 909 377 260 208 119 52 16
9
BanII G_rGCy'C
Cuts at: 0 203 455 564 1602 1741 2034 2465 2753
Size: 203 252 109 1038 139 293 431 288
Cuts at: 2753 2868
Size: 115
Fragments arranged by size:
1038 431 293 288 252 203 139 115
109
BbeI G_GCGC'C
Cuts at: 0 20 2868
Size: 20 2848
BbsI GAAGACnn'nnnn_
Cuts at: 0 160 2868
Size: 160 2708
BbvCI CC'TCA_GC
Cuts at: 0 215 436 2208 2227 2669 2868
Size: 215 221 1772 19 442 199
Fragments arranged by size:
1772 442 221 215 199 19
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 915 949 2868
Size: 915 34 1919
Fragments arranged by size:
1919 915 34
BclI T'GATC_A
Cuts at: 0 2330 2868
Size: 2330 538
BglI GCCn_nnn'nGGC
Cuts at: 0 60 1030 1096 2024 2063 2868
Size: 60 970 66 928 39 805
Fragments arranged by size:
970 928 805 66 60 39
BglII A'GATC_T
Cuts at: 0 490 2868
Size: 490 2378
BlpI GC'TnA_GC
Cuts at: 0 197 388 2868
Size: 197 191 2480
Fragments arranged by size:
2480 197 191
Bme1580I G_kGCm'C
Cuts at: 0 1136 1775 1825 2034 2465 2864 2868
Size: 1136 639 50 209 431 399 4
Fragments arranged by size:
1136 639 431 399 209 50 4
BmgBI CAC'GTC
Cuts at: 0 1830 2868
Size: 1830 1038
BmrI ACTGGGnnnn_n'
Cuts at: 0 567 1139 2593 2868
Size: 567 572 1454 275
Fragments arranged by size:
1454 572 567 275
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2522 2868
Size: 2522 346
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 262 998 1777 2138 2369 2544 2737 2868
Size: 262 736 779 361 231 175 193 131
Fragments arranged by size:
779 736 361 262 231 193 175 131
Bpu10I CC'TnA_GC
Cuts at: 0 202 215 436 2208 2227 2434 2669 2868
Size: 202 13 221 1772 19 207 235 199
Fragments arranged by size:
1772 235 221 207 202 199 19 13
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 487 2185 2710 2868
Size: 487 1698 525 158
Fragments arranged by size:
1698 525 487 158
BsaI GGTCTCn'nnnn_
Cuts at: 0 680 2474 2868
Size: 680 1794 394
Fragments arranged by size:
1794 680 394
BsaHI Gr'CG_yC
Cuts at: 0 17 695 2308 2868
Size: 17 678 1613 560
Fragments arranged by size:
1613 678 560 17
BsaWI w'CCGG_w
Cuts at: 0 761 2340 2868
Size: 761 1579 528
Fragments arranged by size:
1579 761 528
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 2111 2141 2868
Size: 2111 30 727
Fragments arranged by size:
2111 727 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 995 1861 1864 2360 2495 2868
Size: 995 866 3 496 135 373
Fragments arranged by size:
995 866 496 373 135 3
BseYI C'CCAG_C
Cuts at: 0 2027 2363 2868
Size: 2027 336 505
Fragments arranged by size:
2027 505 336
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1950 2399 2868
Size: 1950 449 469
Fragments arranged by size:
1950 469 449
BsiEI CG_ry'CG
Cuts at: 0 64 2572 2868
Size: 64 2508 296
Fragments arranged by size:
2508 296 64
BsiHKAI G_wGCw'C
Cuts at: 0 203 536 964 1374 1741 2868
Size: 203 333 428 410 367 1127
Fragments arranged by size:
1127 428 410 367 333 203
BsmI GAATG_Cn'
Cuts at: 0 1011 2868
Size: 1011 1857
Bsp1286I G_dGCh'C
Cuts at: 0 203 455 536 564 964 1136 1374 1602
Size: 203 252 81 28 400 172 238 228
Cuts at: 1602 1741 1775 1825 2034 2465 2753 2864 2868
Size: 139 34 50 209 431 288 111 4
Fragments arranged by size:
431 400 288 252 238 228 209 203
172 139 111 81 50 34 28 4
BspEI T'CCGG_A
Cuts at: 0 761 2340 2868
Size: 761 1579 528
Fragments arranged by size:
1579 761 528
BspHI T'CATG_A
Cuts at: 0 1793 2868
Size: 1793 1075
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1614 2421 2753 2868
Size: 1614 807 332 115
Fragments arranged by size:
1614 807 332 115
BsrDI GCAATG_nn'
Cuts at: 0 1338 1983 2868
Size: 1338 645 885
Fragments arranged by size:
1338 885 645
BsrFI r'CCGG_y
Cuts at: 0 2513 2868
Size: 2513 355
BssSI C'ACGA_G
Cuts at: 0 649 772 1036 2868
Size: 649 123 264 1832
Fragments arranged by size:
1832 649 264 123
BstAPI GCAn_nnn'nTGC
Cuts at: 0 2695 2793 2868
Size: 2695 98 75
Fragments arranged by size:
2695 98 75
BstEII G'GTnAC_C
Cuts at: 0 301 2868
Size: 301 2567
BstXI CCAn_nnnn'nTGG
Cuts at: 0 939 1017 1054 1507 2868
Size: 939 78 37 453 1361
Fragments arranged by size:
1361 939 453 78 37
BstYI r'GATC_y
Cuts at: 0 490 1724 1808 2868
Size: 490 1234 84 1060
Fragments arranged by size:
1234 1060 490 84
Bsu36I CC'TnA_GG
Cuts at: 0 1351 1844 2868
Size: 1351 493 1024
Fragments arranged by size:
1351 1024 493
BtgI C'CryG_G
Cuts at: 0 64 348 795 921 1028 1983 2058 2713
Size: 64 284 447 126 107 955 75 655
Cuts at: 2713 2868
Size: 155
Fragments arranged by size:
955 655 447 284 155 126 107 75
64
BtsI GCAGTG_nn'
Cuts at: 0 336 1447 1479 1579 1933 2013 2419 2644
Size: 336 1111 32 100 354 80 406 225
Cuts at: 2644 2868
Size: 224
Fragments arranged by size:
1111 406 354 336 225 224 100 80
32
DraI TTT'AAA
Cuts at: 0 620 2868
Size: 620 2248
DrdI GACnn_nn'nnGTC
Cuts at: 0 372 2490 2868
Size: 372 2118 378
Fragments arranged by size:
2118 378 372
EaeI y'GGCC_r
Cuts at: 0 61 953 1088 1527 1980 2129 2515 2569
Size: 61 892 135 439 453 149 386 54
Cuts at: 2569 2868
Size: 299
Fragments arranged by size:
892 453 439 386 299 149 135 61
54
EagI C'GGCC_G
Cuts at: 0 61 2569 2868
Size: 61 2508 299
Fragments arranged by size:
2508 299 61
EarI CTCTTCn'nnn_
Cuts at: 0 569 1280 1301 2868
Size: 569 711 21 1567
Fragments arranged by size:
1567 711 569 21
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1378 2868
Size: 1378 1490
EcoICRI GAG'CTC
Cuts at: 0 201 1739 2868
Size: 201 1538 1129
Fragments arranged by size:
1538 1129 201
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 143 262 550 998 1777 1976 2138 2235
Size: 143 119 288 448 779 199 162 97
Cuts at: 2235 2369 2544 2737 2868
Size: 134 175 193 131
Fragments arranged by size:
779 448 288 199 193 175 162 143
134 131 119 97
EcoO109I rG'GnC_Cy
Cuts at: 0 1415 1591 1840 2031 2104 2405 2461 2868
Size: 1415 176 249 191 73 301 56 407
Fragments arranged by size:
1415 407 301 249 191 176 73 56
HaeII r_GCGC'y
Cuts at: 0 20 2868
Size: 20 2848
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 215 247 607 639 1069 1101 1716 1748
Size: 215 32 360 32 430 32 615 32
Cuts at: 1748 2088 2120 2322 2354 2868
Size: 340 32 202 32 514
Fragments arranged by size:
615 514 430 360 340 215 202 32
32 32 32 32 32
HincII GTy'rAC
Cuts at: 0 987 2868
Size: 987 1881
KasI G'GCGC_C
Cuts at: 0 16 2868
Size: 16 2852
KpnI G_GTAC'C
Cuts at: 0 1514 2744 2868
Size: 1514 1230 124
Fragments arranged by size:
1514 1230 124
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 835 1241 1787 2868
Size: 835 406 546 1081
Fragments arranged by size:
1081 835 546 406
MscI TGG'CCA
Cuts at: 0 955 1982 2131 2868
Size: 955 1027 149 737
Fragments arranged by size:
1027 955 737 149
MslI CAynn'nnrTG
Cuts at: 0 910 955 1015 1234 2137 2158 2868
Size: 910 45 60 219 903 21 710
Fragments arranged by size:
910 903 710 219 60 45 21
MspA1I CmG'CkG
Cuts at: 0 66 1123 1584 2270 2868
Size: 66 1057 461 686 598
Fragments arranged by size:
1057 686 598 461 66
NaeI GCC'GGC
Cuts at: 0 2515 2868
Size: 2515 353
NarI GG'CG_CC
Cuts at: 0 17 2868
Size: 17 2851
NcoI C'CATG_G
Cuts at: 0 1028 1983 2868
Size: 1028 955 885
Fragments arranged by size:
1028 955 885
NgoMIV G'CCGG_C
Cuts at: 0 2513 2868
Size: 2513 355
NspI r_CATG'y
Cuts at: 0 807 915 1224 1554 1863 2868
Size: 807 108 309 330 309 1005
Fragments arranged by size:
1005 807 330 309 309 108
PasI CC'CwG_GG
Cuts at: 0 1870 2313 2458 2868
Size: 1870 443 145 410
Fragments arranged by size:
1870 443 410 145
PciI A'CATG_T
Cuts at: 0 803 2868
Size: 803 2065
PflMI CCAn_nnn'nTGG
Cuts at: 0 557 1041 2868
Size: 557 484 1827
Fragments arranged by size:
1827 557 484
PfoI T'CCnGG_A
Cuts at: 0 233 1451 2494 2868
Size: 233 1218 1043 374
Fragments arranged by size:
1218 1043 374 233
PpuMI rG'GwC_Cy
Cuts at: 0 1415 1591 1840 2104 2405 2868
Size: 1415 176 249 264 301 463
Fragments arranged by size:
1415 463 301 264 249 176
PspOMI G'GGCC_C
Cuts at: 0 2030 2461 2868
Size: 2030 431 407
Fragments arranged by size:
2030 431 407
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 125 157 2868
Size: 125 32 2711
Fragments arranged by size:
2711 125 32
PstI C_TGCA'G
Cuts at: 0 53 128 1077 1193 2176 2306 2868
Size: 53 75 949 116 983 130 562
Fragments arranged by size:
983 949 562 130 116 75 53
PvuII CAG'CTG
Cuts at: 0 1123 1584 2868
Size: 1123 461 1284
Fragments arranged by size:
1284 1123 461
SacI G_AGCT'C
Cuts at: 0 203 1741 2868
Size: 203 1538 1127
Fragments arranged by size:
1538 1127 203
SacII CC_GC'GG
Cuts at: 0 67 2868
Size: 67 2801
SanDI GG'GwC_CC
Cuts at: 0 1415 1591 2405 2868
Size: 1415 176 814 463
Fragments arranged by size:
1415 814 463 176
SapI GCTCTTCn'nnn_
Cuts at: 0 569 2868
Size: 569 2299
SfcI C'TryA_G
Cuts at: 0 49 124 1073 1189 2172 2302 2868
Size: 49 75 949 116 983 130 566
Fragments arranged by size:
983 949 566 130 116 75 49
SfoI GGC'GCC
Cuts at: 0 18 2868
Size: 18 2850
SmaI CCC'GGG
Cuts at: 0 515 2466 2868
Size: 515 1951 402
Fragments arranged by size:
1951 515 402
SmlI C'TyrA_G
Cuts at: 0 502 2200 2725 2868
Size: 502 1698 525 143
Fragments arranged by size:
1698 525 502 143
StuI AGG'CCT
Cuts at: 0 1534 2502 2565 2868
Size: 1534 968 63 303
Fragments arranged by size:
1534 968 303 63
StyI C'CwwG_G
Cuts at: 0 1028 1983 2833 2868
Size: 1028 955 850 35
Fragments arranged by size:
1028 955 850 35
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1097 1840 2868
Size: 1097 743 1028
Fragments arranged by size:
1097 1028 743
TatI w'GTAC_w
Cuts at: 0 2162 2868
Size: 2162 706
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 792 824 2111 2143 2868
Size: 792 32 1287 32 725
Fragments arranged by size:
1287 792 725 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 2487 2868
Size: 2487 381
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 324 633 936 2139 2868
Size: 324 309 303 1203 729
Fragments arranged by size:
1203 729 324 309 303
XmaI C'CCGG_G
Cuts at: 0 513 2464 2868
Size: 513 1951 404
Fragments arranged by size:
1951 513 404
Enzymes that do cut:
AarI AccI Acc65I AcuI AflIII AlfI AlwNI ApaI
AseI AvaI BanI BanII BbeI BbsI BbvCI BcgI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrDI BsrFI BssSI BstAPI BstEII BstXI BstYI
Bsu36I BtgI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I HaeII Hin4I HincII KasI
KpnI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PasI PciI PflMI PfoI PpuMI PspOMI
PsrI PstI PvuII SacI SacII SanDI SapI SfcI
SfoI SmaI SmlI StuI StyI TaqII TatI TstI
Tth111I XcmI XmaI
Enzymes that do not cut:
AatII AclI AfeI AflII AgeI AhdI AleI AloI
ApaLI ApoI AscI AsiSI AvrII BaeI BamHI BciVI
BfrBI BmtI BsaAI BsaBI BsiWI BsmBI BsrBI BsrGI
BssHII BstBI BstZ17I BtgZI ClaI CspCI DraIII EcoNI
EcoRI EcoRV FalI FseI FspI FspAI HindIII HpaI
MfeI MluI NdeI NheI NotI NruI NsiI PacI
PmeI PmlI PpiI PshAI PsiI PspXI PvuI RsrII
SalI SbfI ScaI SexAI SfiI SgfI SgrAI SnaBI
SpeI SphI SrfI SspI SwaI XbaI XhoI XmnI
ZraI