Restriction map for
pF1KB7299
(Linear) (Six-base) MAPSORT of: KIBB7299.seq Check: 4677 from: 1 to: 3672
KIBB7299.orf
With 182 enzymes: SgfI *
March 3, 2008 11:00 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 48 84 2970 3672
Size: 48 36 2886 702
Fragments arranged by size:
2886 702 48 36
AatII G_ACGT'C
Cuts at: 0 2201 3672
Size: 2201 1471
AccI GT'mk_AC
Cuts at: 0 893 3672
Size: 893 2779
Acc65I G'GTAC_C
Cuts at: 0 3597 3672
Size: 3597 75
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1915 2719 3115 3672
Size: 1915 804 396 557
Fragments arranged by size:
1915 804 557 396
AfeI AGC'GCT
Cuts at: 0 835 3136 3672
Size: 835 2301 536
Fragments arranged by size:
2301 835 536
AflII C'TTAA_G
Cuts at: 0 1083 3672
Size: 1083 2589
AflIII A'CryG_T
Cuts at: 0 221 1343 1697 2824 3196 3672
Size: 221 1122 354 1127 372 476
Fragments arranged by size:
1127 1122 476 372 354 221
AleI CACnn'nnGTG
Cuts at: 0 78 940 3002 3672
Size: 78 862 2062 670
Fragments arranged by size:
2062 862 670 78
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 92 96 2615 3672
Size: 92 4 2519 1057
Fragments arranged by size:
2519 1057 92 4
AlwNI CAG_nnn'CTG
Cuts at: 0 109 1802 3072 3165 3459 3672
Size: 109 1693 1270 93 294 213
Fragments arranged by size:
1693 1270 294 213 109 93
ApaI G_GGCC'C
Cuts at: 0 2981 3672
Size: 2981 691
ApaLI G'TGCA_C
Cuts at: 0 2859 2872 3411 3672
Size: 2859 13 539 261
Fragments arranged by size:
2859 539 261 13
ApoI r'AATT_y
Cuts at: 0 1441 1657 2428 3672
Size: 1441 216 771 1244
Fragments arranged by size:
1441 1244 771 216
AseI AT'TA_AT
Cuts at: 0 1256 3672
Size: 1256 2416
AvaI C'yCGr_G
Cuts at: 0 117 1922 2473 2544 3375 3672
Size: 117 1805 551 71 831 297
Fragments arranged by size:
1805 831 551 297 117 71
BanI G'GyrC_C
Cuts at: 0 72 1625 1703 2757 2836 3347 3597 3672
Size: 72 1553 78 1054 79 511 250 75
Fragments arranged by size:
1553 1054 511 250 79 78 75 72
BanII G_rGCy'C
Cuts at: 0 118 632 1193 1882 2313 2474 2981 3101
Size: 118 514 561 689 431 161 507 120
Cuts at: 3101 3672
Size: 571
Fragments arranged by size:
689 571 561 514 507 431 161 120
118
BbsI GAAGACnn'nnnn_
Cuts at: 0 756 791 1395 1769 2201 3672
Size: 756 35 604 374 432 1471
Fragments arranged by size:
1471 756 604 432 374 35
BbvCI CC'TCA_GC
Cuts at: 0 2912 3672
Size: 2912 760
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2427 2461 3432 3466 3672
Size: 2427 34 971 34 206
Fragments arranged by size:
2427 971 206 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 1517 2329 3672
Size: 1517 812 1343
Fragments arranged by size:
1517 1343 812
BclI T'GATC_A
Cuts at: 0 1476 3672
Size: 1476 2196
BfrBI ATG'CAT
Cuts at: 0 3114 3672
Size: 3114 558
BglI GCCn_nnn'nGGC
Cuts at: 0 70 3517 3672
Size: 70 3447 155
Fragments arranged by size:
3447 155 70
Bme1580I G_kGCm'C
Cuts at: 0 269 2399 2440 2841 2863 2876 2911 2981
Size: 269 2130 41 401 22 13 35 70
Cuts at: 2981 3415 3672
Size: 434 257
Fragments arranged by size:
2130 434 401 269 257 70 41 35
22 13
BmgBI CAC'GTC
Cuts at: 0 2825 3672
Size: 2825 847
BmrI ACTGGGnnnn_n'
Cuts at: 0 3177 3672
Size: 3177 495
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 3288 3672
Size: 3288 384
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 294 1779 2327 2826 2910 3328 3672
Size: 294 1485 548 499 84 418 344
Fragments arranged by size:
1485 548 499 418 344 294 84
Bpu10I CC'TnA_GC
Cuts at: 0 2912 3672
Size: 2912 760
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 651 1442 1997 3672
Size: 651 791 555 1675
Fragments arranged by size:
1675 791 651 555
BsaI GGTCTCn'nnnn_
Cuts at: 0 192 871 2254 2687 3410 3672
Size: 192 679 1383 433 723 262
Fragments arranged by size:
1383 723 679 433 262 192
BsaAI yAC'GTr
Cuts at: 0 222 606 942 3672
Size: 222 384 336 2730
Fragments arranged by size:
2730 384 336 222
BsaHI Gr'CG_yC
Cuts at: 0 2198 3672
Size: 2198 1474
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 451 481 1799 1829 2512 2542 3672
Size: 451 30 1318 30 683 30 1130
Fragments arranged by size:
1318 1130 683 451 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 125 459 3672
Size: 125 334 3213
Fragments arranged by size:
3213 334 125
BseYI C'CCAG_C
Cuts at: 0 770 2603 2608 2785 3301 3463 3602 3672
Size: 770 1833 5 177 516 162 139 70
Fragments arranged by size:
1833 770 516 177 162 139 70 5
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 394 427 682 1233 1821 2217 2875 3072
Size: 394 33 255 551 588 396 658 197
Cuts at: 3072 3472 3478 3636 3672
Size: 400 6 158 36
Fragments arranged by size:
658 588 551 400 396 394 255 197
158 36 33 6
BsiEI CG_ry'CG
Cuts at: 0 154 2421 3475 3672
Size: 154 2267 1054 197
Fragments arranged by size:
2267 1054 197 154
BsiHKAI G_wGCw'C
Cuts at: 0 947 1964 2644 2863 2876 2995 3046 3190
Size: 947 1017 680 219 13 119 51 144
Cuts at: 3190 3286 3415 3672
Size: 96 129 257
Fragments arranged by size:
1017 947 680 257 219 144 129 119
96 51 13
BsmBI CGTCTCn'nnnn_
Cuts at: 0 2346 3672
Size: 2346 1326
Bsp1286I G_dGCh'C
Cuts at: 0 118 269 632 947 1193 1882 1964 2313
Size: 118 151 363 315 246 689 82 349
Cuts at: 2313 2399 2440 2474 2644 2841 2863 2876 2911
Size: 86 41 34 170 197 22 13 35
Cuts at: 2911 2981 2995 3046 3101 3190 3286 3415 3672
Size: 70 14 51 55 89 96 129 257
Fragments arranged by size:
689 363 349 315 257 246 197 170
151 129 118 96 89 86 82 70
55 51 41 35 34 22 14 13
BspHI T'CATG_A
Cuts at: 0 1137 1326 3672
Size: 1137 189 2346
Fragments arranged by size:
2346 1137 189
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 48 84 496 1810 2970 3672
Size: 48 36 412 1314 1160 702
Fragments arranged by size:
1314 1160 702 412 48 36
BsrBI CCG'CTC
Cuts at: 0 150 1456 3672
Size: 150 1306 2216
Fragments arranged by size:
2216 1306 150
BsrDI GCAATG_nn'
Cuts at: 0 1007 1289 1401 2373 3542 3672
Size: 1007 282 112 972 1169 130
Fragments arranged by size:
1169 1007 972 282 130 112
BsrFI r'CCGG_y
Cuts at: 0 2420 3470 3474 3672
Size: 2420 1050 4 198
Fragments arranged by size:
2420 1050 198 4
BsrGI T'GTAC_A
Cuts at: 0 611 3672
Size: 611 3061
BssHII G'CGCG_C
Cuts at: 0 11 13 3672
Size: 11 2 3659
Fragments arranged by size:
3659 11 2
BstAPI GCAn_nnn'nTGC
Cuts at: 0 3072 3641 3653 3672
Size: 3072 569 12 19
Fragments arranged by size:
3072 569 19 12
BstEII G'GTnAC_C
Cuts at: 0 660 1412 2292 3672
Size: 660 752 880 1380
Fragments arranged by size:
1380 880 752 660
BstXI CCAn_nnnn'nTGG
Cuts at: 0 640 1303 1636 2966 3672
Size: 640 663 333 1330 706
Fragments arranged by size:
1330 706 663 640 333
BstYI r'GATC_y
Cuts at: 0 330 619 2179 3672
Size: 330 289 1560 1493
Fragments arranged by size:
1560 1493 330 289
Bsu36I CC'TnA_GG
Cuts at: 0 538 3480 3672
Size: 538 2942 192
Fragments arranged by size:
2942 538 192
BtgI C'CryG_G
Cuts at: 0 86 143 915 1755 2405 2795 3672
Size: 86 57 772 840 650 390 877
Fragments arranged by size:
877 840 772 650 390 86 57
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1838 3672
Size: 1838 1834
BtsI GCAGTG_nn'
Cuts at: 0 269 2863 3035 3166 3460 3646 3672
Size: 269 2594 172 131 294 186 26
Fragments arranged by size:
2594 294 269 186 172 131 26
DraI TTT'AAA
Cuts at: 0 1599 3672
Size: 1599 2073
DraIII CAC_nnn'GTG
Cuts at: 0 1550 3672
Size: 1550 2122
EaeI y'GGCC_r
Cuts at: 0 62 151 918 1758 2418 2897 3056 3416
Size: 62 89 767 840 660 479 159 360
Cuts at: 3416 3472 3672
Size: 56 200
Fragments arranged by size:
840 767 660 479 360 200 159 89
62 56
EagI C'GGCC_G
Cuts at: 0 151 2418 3472 3672
Size: 151 2267 1054 200
Fragments arranged by size:
2267 1054 200 151
EarI CTCTTCn'nnn_
Cuts at: 0 252 293 515 796 845 3672
Size: 252 41 222 281 49 2827
Fragments arranged by size:
2827 281 252 222 49 41
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 267 3162 3672
Size: 267 2895 510
Fragments arranged by size:
2895 510 267
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 294 1779 1915 2327 2719 2826 2910 3115
Size: 294 1485 136 412 392 107 84 205
Cuts at: 3115 3328 3672
Size: 213 344
Fragments arranged by size:
1485 412 392 344 294 213 205 136
107 84
EcoNI CCTnn'n_nnAGG
Cuts at: 0 2928 3672
Size: 2928 744
EcoO109I rG'GnC_Cy
Cuts at: 0 91 654 848 1741 1799 2978 3370 3672
Size: 91 563 194 893 58 1179 392 302
Fragments arranged by size:
1179 893 563 392 302 194 91 58
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 837 3672
Size: 837 2835
FseI GG_CCGG'CC
Cuts at: 0 3474 3478 3672
Size: 3474 4 194
Fragments arranged by size:
3474 194 4
FspI TGC'GCA
Cuts at: 0 2365 3672
Size: 2365 1307
HaeII r_GCGC'y
Cuts at: 0 40 109 837 2975 3138 3672
Size: 40 69 728 2138 163 534
Fragments arranged by size:
2138 728 534 163 69 40
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 172 204 208 240 268 300 799 831
Size: 172 32 4 32 28 32 499 32
Cuts at: 831 986 1018 3672
Size: 155 32 2654
Fragments arranged by size:
2654 499 172 155 32 32 32 32
32 28 4
HincII GTy'rAC
Cuts at: 0 204 894 1935 3672
Size: 204 690 1041 1737
Fragments arranged by size:
1737 1041 690 204
KpnI G_GTAC'C
Cuts at: 0 3601 3672
Size: 3601 71
MfeI C'AATT_G
Cuts at: 0 1013 3672
Size: 1013 2659
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1748 2139 2800 3672
Size: 1748 391 661 872
Fragments arranged by size:
1748 872 661 391
MscI TGG'CCA
Cuts at: 0 920 1760 3672
Size: 920 840 1912
Fragments arranged by size:
1912 920 840
MslI CAynn'nnrTG
Cuts at: 0 78 743 857 920 940 2359 2964 3002
Size: 78 665 114 63 20 1419 605 38
Cuts at: 3002 3672
Size: 670
Fragments arranged by size:
1419 670 665 605 114 78 63 38
20
MspA1I CmG'CkG
Cuts at: 0 100 145 249 1247 2789 3069 3386 3422
Size: 100 45 104 998 1542 280 317 36
Cuts at: 3422 3672
Size: 250
Fragments arranged by size:
1542 998 317 280 250 104 100 45
36
NaeI GCC'GGC
Cuts at: 0 3472 3476 3672
Size: 3472 4 196
Fragments arranged by size:
3472 196 4
NcoI C'CATG_G
Cuts at: 0 86 915 1755 3672
Size: 86 829 840 1917
Fragments arranged by size:
1917 840 829 86
NgoMIV G'CCGG_C
Cuts at: 0 3470 3474 3672
Size: 3470 4 198
Fragments arranged by size:
3470 198 4
NsiI A_TGCA'T
Cuts at: 0 3116 3672
Size: 3116 556
NspI r_CATG'y
Cuts at: 0 768 1347 1590 1701 3200 3672
Size: 768 579 243 111 1499 472
Fragments arranged by size:
1499 768 579 472 243 111
PasI CC'CwG_GG
Cuts at: 0 2981 3672
Size: 2981 691
PciI A'CATG_T
Cuts at: 0 1343 1697 3196 3672
Size: 1343 354 1499 476
Fragments arranged by size:
1499 1343 476 354
PflMI CCAn_nnn'nTGG
Cuts at: 0 1858 3672
Size: 1858 1814
PfoI T'CCnGG_A
Cuts at: 0 1177 1795 3672
Size: 1177 618 1877
Fragments arranged by size:
1877 1177 618
PpuMI rG'GwC_Cy
Cuts at: 0 91 654 848 1799 3370 3672
Size: 91 563 194 951 1571 302
Fragments arranged by size:
1571 951 563 302 194 91
PspOMI G'GGCC_C
Cuts at: 0 2977 3672
Size: 2977 695
PspXI vC'TCGA_Gb
Cuts at: 0 3375 3672
Size: 3375 297
PstI C_TGCA'G
Cuts at: 0 1423 2818 3161 3672
Size: 1423 1395 343 511
Fragments arranged by size:
1423 1395 511 343
PvuII CAG'CTG
Cuts at: 0 1247 2789 3069 3386 3422 3672
Size: 1247 1542 280 317 36 250
Fragments arranged by size:
1542 1247 317 280 250 36
SacII CC_GC'GG
Cuts at: 0 146 3672
Size: 146 3526
SalI G'TCGA_C
Cuts at: 0 892 3672
Size: 892 2780
SanDI GG'GwC_CC
Cuts at: 0 91 3370 3672
Size: 91 3279 302
Fragments arranged by size:
3279 302 91
SapI GCTCTTCn'nnn_
Cuts at: 0 252 3672
Size: 252 3420
SexAI A'CCwGG_T
Cuts at: 0 182 3593 3672
Size: 182 3411 79
Fragments arranged by size:
3411 182 79
SfcI C'TryA_G
Cuts at: 0 1419 2451 2688 2814 3157 3672
Size: 1419 1032 237 126 343 515
Fragments arranged by size:
1419 1032 515 343 237 126
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 3517 3672
Size: 3517 155
SmaI CCC'GGG
Cuts at: 0 1924 2546 3672
Size: 1924 622 1126
Fragments arranged by size:
1924 1126 622
SmlI C'TyrA_G
Cuts at: 0 666 1083 1457 2012 3375 3672
Size: 666 417 374 555 1363 297
Fragments arranged by size:
1363 666 555 417 374 297
SphI G_CATG'C
Cuts at: 0 1590 3672
Size: 1590 2082
StuI AGG'CCT
Cuts at: 0 2938 3672
Size: 2938 734
StyI C'CwwG_G
Cuts at: 0 86 492 585 779 881 915 1755 2175
Size: 86 406 93 194 102 34 840 420
Cuts at: 2175 3672
Size: 1497
Fragments arranged by size:
1497 840 420 406 194 102 93 86
34
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1230 1768 2599 3672
Size: 1230 538 831 1073
Fragments arranged by size:
1230 1073 831 538
TatI w'GTAC_w
Cuts at: 0 611 3672
Size: 611 3061
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 451 483 1706 1738 1857 1889 2532 2564
Size: 451 32 1223 32 119 32 643 32
Cuts at: 2564 2793 2825 3672
Size: 229 32 847
Fragments arranged by size:
1223 847 643 451 229 119 32 32
32 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 184 2170 3082 3444 3672
Size: 184 1986 912 362 228
Fragments arranged by size:
1986 912 362 228 184
XbaI T'CTAG_A
Cuts at: 0 1032 3672
Size: 1032 2640
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 351 654 1105 2574 2793 3309 3672
Size: 351 303 451 1469 219 516 363
Fragments arranged by size:
1469 516 451 363 351 303 219
XhoI C'TCGA_G
Cuts at: 0 3375 3672
Size: 3375 297
XmaI C'CCGG_G
Cuts at: 0 1922 2544 3672
Size: 1922 622 1128
Fragments arranged by size:
1922 1128 622
XmnI GAAnn'nnTTC
Cuts at: 0 3220 3672
Size: 3220 452
ZraI GAC'GTC
Cuts at: 0 2199 3672
Size: 2199 1473
Enzymes that do cut:
AarI AatII AccI Acc65I AcuI AfeI AflII AflIII
AleI AlfI AlwNI ApaI ApaLI ApoI AseI AvaI
BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI
BglI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaHI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmBI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BstAPI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI DraI DraIII EaeI EagI EarI EciI
Eco57MI EcoNI EcoO109I FalI FseI FspI HaeII Hin4I
HincII KpnI MfeI MmeI MscI MslI MspA1I NaeI
NcoI NgoMIV NsiI NspI PasI PciI PflMI PfoI
PpuMI PspOMI PspXI PstI PvuII SacII SalI SanDI
SapI SexAI SfcI SfiI SmaI SmlI SphI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AclI AgeI AhdI AloI AscI AsiSI AvrII BaeI
BamHI BbeI BglII BlpI BmtI BsaBI BsaWI BsiWI
BsmI BspEI BssSI BstBI BstZ17I ClaI CspCI DrdI
EcoICRI EcoRI EcoRV FspAI HindIII HpaI KasI MluI
NarI NdeI NheI NotI NruI PacI PmeI PmlI
PpiI PshAI PsiI PsrI PvuI RsrII SacI SbfI
ScaI SfoI SgfI SgrAI SnaBI SpeI SrfI SspI
SwaI