Restriction map for
pF1KB7289
(Linear) (Six-base) MAPSORT of: KIBB7289.seq Check: 4405 from: 1 to: 2964
KIBB7289.orf
With 182 enzymes: SgfI *
March 3, 2008 11:00 ..
AclI AA'CG_TT
Cuts at: 0 1364 2964
Size: 1364 1600
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 412 504 628 1054 2417 2964
Size: 412 92 124 426 1363 547
Fragments arranged by size:
1363 547 426 412 124 92
AfeI AGC'GCT
Cuts at: 0 1592 2964
Size: 1592 1372
AflIII A'CryG_T
Cuts at: 0 2387 2964
Size: 2387 577
AleI CACnn'nnGTG
Cuts at: 0 1310 1627 2964
Size: 1310 317 1337
Fragments arranged by size:
1337 1310 317
AlwNI CAG_nnn'CTG
Cuts at: 0 483 722 1716 2278 2310 2964
Size: 483 239 994 562 32 654
Fragments arranged by size:
994 654 562 483 239 32
ApaI G_GGCC'C
Cuts at: 0 700 2917 2964
Size: 700 2217 47
Fragments arranged by size:
2217 700 47
ApoI r'AATT_y
Cuts at: 0 742 2964
Size: 742 2222
AscI GG'CGCG_CC
Cuts at: 0 2096 2964
Size: 2096 868
AvaI C'yCGr_G
Cuts at: 0 1355 2113 2964
Size: 1355 758 851
Fragments arranged by size:
1355 851 758
BanI G'GyrC_C
Cuts at: 0 1600 2694 2964
Size: 1600 1094 270
Fragments arranged by size:
1600 1094 270
BanII G_rGCy'C
Cuts at: 0 700 1079 1951 2090 2231 2337 2813 2917
Size: 700 379 872 139 141 106 476 104
Cuts at: 2917 2964
Size: 47
Fragments arranged by size:
872 700 476 379 141 139 106 104
47
BbsI GAAGACnn'nnnn_
Cuts at: 0 247 353 589 2156 2964
Size: 247 106 236 1567 808
Fragments arranged by size:
1567 808 247 236 106
BbvCI CC'TCA_GC
Cuts at: 0 736 1144 2964
Size: 736 408 1820
Fragments arranged by size:
1820 736 408
BciVI GTATCCnnnnn_n'
Cuts at: 0 316 1187 2964
Size: 316 871 1777
Fragments arranged by size:
1777 871 316
BglI GCCn_nnn'nGGC
Cuts at: 0 637 2235 2964
Size: 637 1598 729
Fragments arranged by size:
1598 729 637
BlpI GC'TnA_GC
Cuts at: 0 1160 1792 2428 2964
Size: 1160 632 636 536
Fragments arranged by size:
1160 636 632 536
Bme1580I G_kGCm'C
Cuts at: 0 700 2618 2697 2917 2964
Size: 700 1918 79 220 47
Fragments arranged by size:
1918 700 220 79 47
BmrI ACTGGGnnnn_n'
Cuts at: 0 54 2964
Size: 54 2910
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 894 2964
Size: 894 2070
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 109 234 417 927 1265 1870 2087 2608
Size: 109 125 183 510 338 605 217 521
Cuts at: 2608 2881 2964
Size: 273 83
Fragments arranged by size:
605 521 510 338 273 217 183 125
109 83
Bpu10I CC'TnA_GC
Cuts at: 0 736 1144 2000 2569 2964
Size: 736 408 856 569 395
Fragments arranged by size:
856 736 569 408 395
BsaI GGTCTCn'nnnn_
Cuts at: 0 2776 2964
Size: 2776 188
BsaAI yAC'GTr
Cuts at: 0 1831 2550 2964
Size: 1831 719 414
Fragments arranged by size:
1831 719 414
BsaBI GATnn'nnATC
Cuts at: 0 278 1411 2964
Size: 278 1133 1553
Fragments arranged by size:
1553 1133 278
BsaHI Gr'CG_yC
Cuts at: 0 2576 2964
Size: 2576 388
BsaWI w'CCGG_w
Cuts at: 0 137 2660 2964
Size: 137 2523 304
Fragments arranged by size:
2523 304 137
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1603 1633 2964
Size: 1603 30 1331
Fragments arranged by size:
1603 1331 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 866 2605 2869 2905 2953 2964
Size: 866 1739 264 36 48 11
Fragments arranged by size:
1739 866 264 48 36 11
BseYI C'CCAG_C
Cuts at: 0 463 1944 2077 2325 2917 2964
Size: 463 1481 133 248 592 47
Fragments arranged by size:
1481 592 463 248 133 47
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 72 2485 2653 2964
Size: 72 2413 168 311
Fragments arranged by size:
2413 311 168 72
BsiEI CG_ry'CG
Cuts at: 0 1963 2964
Size: 1963 1001
BsiHKAI G_wGCw'C
Cuts at: 0 2337 2964
Size: 2337 627
Bsp1286I G_dGCh'C
Cuts at: 0 700 1079 1951 2090 2231 2337 2618 2697
Size: 700 379 872 139 141 106 281 79
Cuts at: 2697 2813 2917 2964
Size: 116 104 47
Fragments arranged by size:
872 700 379 281 141 139 116 106
104 79 47
BspEI T'CCGG_A
Cuts at: 0 137 2660 2964
Size: 137 2523 304
Fragments arranged by size:
2523 304 137
BspHI T'CATG_A
Cuts at: 0 1394 2964
Size: 1394 1570
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 2272 2732 2964
Size: 2272 460 232
Fragments arranged by size:
2272 460 232
BsrBI CCG'CTC
Cuts at: 0 1959 2964
Size: 1959 1005
BsrDI GCAATG_nn'
Cuts at: 0 710 1199 2964
Size: 710 489 1765
Fragments arranged by size:
1765 710 489
BsrFI r'CCGG_y
Cuts at: 0 2108 2964
Size: 2108 856
BssHII G'CGCG_C
Cuts at: 0 2013 2096 2964
Size: 2013 83 868
Fragments arranged by size:
2013 868 83
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1170 2964
Size: 1170 1794
BstBI TT'CG_AA
Cuts at: 0 1640 2964
Size: 1640 1324
BstEII G'GTnAC_C
Cuts at: 0 492 873 2964
Size: 492 381 2091
Fragments arranged by size:
2091 492 381
BstXI CCAn_nnnn'nTGG
Cuts at: 0 559 1680 1704 2964
Size: 559 1121 24 1260
Fragments arranged by size:
1260 1121 559 24
BstYI r'GATC_y
Cuts at: 0 353 1553 2964
Size: 353 1200 1411
Fragments arranged by size:
1411 1200 353
Bsu36I CC'TnA_GG
Cuts at: 0 2729 2964
Size: 2729 235
BtgI C'CryG_G
Cuts at: 0 260 683 2964
Size: 260 423 2281
Fragments arranged by size:
2281 423 260
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1090 2964
Size: 1090 1874
BtsI GCAGTG_nn'
Cuts at: 0 84 678 965 986 2316 2964
Size: 84 594 287 21 1330 648
Fragments arranged by size:
1330 648 594 287 84 21
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 25 60 870 905 2964
Size: 25 35 810 35 2059
Fragments arranged by size:
2059 810 35 35 25
EaeI y'GGCC_r
Cuts at: 0 186 638 1418 1505 1694 2247 2897 2964
Size: 186 452 780 87 189 553 650 67
Fragments arranged by size:
780 650 553 452 189 186 87 67
EarI CTCTTCn'nnn_
Cuts at: 0 523 535 1390 2219 2393 2882 2964
Size: 523 12 855 829 174 489 82
Fragments arranged by size:
855 829 523 489 174 82 12
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 2629 2964
Size: 2629 335
EcoICRI GAG'CTC
Cuts at: 0 2335 2964
Size: 2335 629
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 109 234 412 417 504 628 927 1054
Size: 109 125 178 5 87 124 299 127
Cuts at: 1054 1265 1870 2087 2417 2608 2881 2964
Size: 211 605 217 330 191 273 83
Fragments arranged by size:
605 330 299 273 217 211 191 178
127 125 124 109 87 83 5
EcoNI CCTnn'n_nnAGG
Cuts at: 0 319 2728 2964
Size: 319 2409 236
Fragments arranged by size:
2409 319 236
EcoO109I rG'GnC_Cy
Cuts at: 0 120 151 696 697 1140 1974 2913 2914
Size: 120 31 545 1 443 834 939 1
Cuts at: 2914 2964
Size: 50
Fragments arranged by size:
939 834 545 443 120 50 31 1
1
EcoRV GAT'ATC
Cuts at: 0 795 2964
Size: 795 2169
FseI GG_CCGG'CC
Cuts at: 0 2112 2964
Size: 2112 852
HaeII r_GCGC'y
Cuts at: 0 527 938 1594 1989 2964
Size: 527 411 656 395 975
Fragments arranged by size:
975 656 527 411 395
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 2282 2314 2374 2406 2661 2693 2964
Size: 2282 32 60 32 255 32 271
Fragments arranged by size:
2282 271 255 60 32 32 32
HincII GTy'rAC
Cuts at: 0 1362 1920 2964
Size: 1362 558 1044
Fragments arranged by size:
1362 1044 558
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1943 2024 2831 2964
Size: 1943 81 807 133
Fragments arranged by size:
1943 807 133 81
MscI TGG'CCA
Cuts at: 0 188 640 1420 1507 1696 2249 2964
Size: 188 452 780 87 189 553 715
Fragments arranged by size:
780 715 553 452 189 188 87
MslI CAynn'nnrTG
Cuts at: 0 229 682 751 1310 1426 1456 1507 1627
Size: 229 453 69 559 116 30 51 120
Cuts at: 1627 1678 2964
Size: 51 1286
Fragments arranged by size:
1286 559 453 229 120 116 69 51
51 30
MspA1I CmG'CkG
Cuts at: 0 395 1339 1627 2427 2481 2964
Size: 395 944 288 800 54 483
Fragments arranged by size:
944 800 483 395 288 54
NaeI GCC'GGC
Cuts at: 0 2110 2964
Size: 2110 854
NdeI CA'TA_TG
Cuts at: 0 1863 2964
Size: 1863 1101
NgoMIV G'CCGG_C
Cuts at: 0 2108 2964
Size: 2108 856
NspI r_CATG'y
Cuts at: 0 2391 2964
Size: 2391 573
PciI A'CATG_T
Cuts at: 0 2387 2964
Size: 2387 577
PflMI CCAn_nnn'nTGG
Cuts at: 0 315 2028 2484 2964
Size: 315 1713 456 480
Fragments arranged by size:
1713 480 456 315
PfoI T'CCnGG_A
Cuts at: 0 1179 2964
Size: 1179 1785
PspOMI G'GGCC_C
Cuts at: 0 696 2913 2964
Size: 696 2217 51
Fragments arranged by size:
2217 696 51
PstI C_TGCA'G
Cuts at: 0 479 1721 1973 1985 2283 2712 2964
Size: 479 1242 252 12 298 429 252
Fragments arranged by size:
1242 479 429 298 252 252 12
PvuII CAG'CTG
Cuts at: 0 395 1339 1627 2427 2481 2964
Size: 395 944 288 800 54 483
Fragments arranged by size:
944 800 483 395 288 54
SacI G_AGCT'C
Cuts at: 0 2337 2964
Size: 2337 627
SapI GCTCTTCn'nnn_
Cuts at: 0 2219 2964
Size: 2219 745
SbfI CC_TGCA'GG
Cuts at: 0 2283 2964
Size: 2283 681
ScaI AGT'ACT
Cuts at: 0 1275 2964
Size: 1275 1689
SfcI C'TryA_G
Cuts at: 0 475 1717 1969 1981 2279 2596 2708 2964
Size: 475 1242 252 12 298 317 112 256
Fragments arranged by size:
1242 475 317 298 256 252 112 12
SmaI CCC'GGG
Cuts at: 0 1357 2964
Size: 1357 1607
SpeI A'CTAG_T
Cuts at: 0 504 2964
Size: 504 2460
StuI AGG'CCT
Cuts at: 0 433 2410 2739 2964
Size: 433 1977 329 225
Fragments arranged by size:
1977 433 329 225
StyI C'CwwG_G
Cuts at: 0 641 1776 2529 2964
Size: 641 1135 753 435
Fragments arranged by size:
1135 753 641 435
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1287 2964
Size: 1287 1677
TatI w'GTAC_w
Cuts at: 0 1273 2964
Size: 1273 1691
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 311 343 1056 1088 1603 1635 2964
Size: 311 32 713 32 515 32 1329
Fragments arranged by size:
1329 713 515 311 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 409 2964
Size: 409 2555
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 258 1011 2376 2964
Size: 258 753 1365 588
Fragments arranged by size:
1365 753 588 258
XmaI C'CCGG_G
Cuts at: 0 1355 2964
Size: 1355 1609
Enzymes that do cut:
AclI AcuI AfeI AflIII AleI AlwNI ApaI ApoI
AscI AvaI BanI BanII BbsI BbvCI BciVI BglI
BlpI Bme1580I BmrI BplI BpmI Bpu10I BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssHII BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI CspCI EaeI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRV FseI HaeII Hin4I HincII MmeI
MscI MslI MspA1I NaeI NdeI NgoMIV NspI PciI
PflMI PfoI PspOMI PstI PvuII SacI SapI SbfI
ScaI SfcI SmaI SpeI StuI StyI TaqII TatI
TstI Tth111I XcmI XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AflII AgeI AhdI AlfI
AloI ApaLI AseI AsiSI AvrII BaeI BamHI BbeI
BcgI BclI BfrBI BglII BmgBI BmtI BpuEI BsiWI
BsmI BsmBI BsrGI BssSI BstZ17I ClaI DraI DraIII
DrdI EagI EcoRI FalI FspI FspAI HindIII HpaI
KasI KpnI MfeI MluI NarI NcoI NheI NotI
NruI NsiI PacI PasI PmeI PmlI PpiI PpuMI
PshAI PsiI PspXI PsrI PvuI RsrII SacII SalI
SanDI SexAI SfiI SfoI SgfI SgrAI SmlI SnaBI
SphI SrfI SspI SwaI XbaI XhoI XmnI ZraI