(Linear) (Six-base) MAPSORT of: KIBB7276.seq  Check: 5408  from: 1  to: 1146



KIBB7276.orf

 With 182 enzymes: SgfI * 

                        March 2, 2010 14:27  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     433     693     946    1146 
   Size:        433     260     253     200

  Fragments arranged by size: 

                 433     260     253     200
 
AlwNI CAG_nnn'CTG

Cuts at:      0     351    1146 
   Size:        351     795
 
ApoI r'AATT_y

Cuts at:      0     453     733     855    1138    1146 
   Size:        453     280     122     283       8

  Fragments arranged by size: 

                 453     283     280     122       8
 
AvaI C'yCGr_G

Cuts at:      0     413    1146 
   Size:        413     733
 
BfrBI ATG'CAT

Cuts at:      0     187    1146 
   Size:        187     959
 
BsaAI yAC'GTr

Cuts at:      0     888    1146 
   Size:        888     258
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     781    1146 
   Size:        781     365
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1055    1146 
   Size:       1055      91
 
BsmI GAATG_Cn'

Cuts at:      0     474    1146 
   Size:        474     672
 
BsrDI GCAATG_nn'

Cuts at:      0     645     959    1146 
   Size:        645     314     187

  Fragments arranged by size: 

                 645     314     187
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     874    1146 
   Size:        874     272
 
BtsI GCAGTG_nn'

Cuts at:      0    1038    1146 
   Size:       1038     108
 
EaeI y'GGCC_r

Cuts at:      0     326    1146 
   Size:        326     820
 
EarI CTCTTCn'nnn_

Cuts at:      0     758    1146 
   Size:        758     388
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     433     693     946    1146 
   Size:        433     260     253     200

  Fragments arranged by size: 

                 433     260     253     200
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     687    1146 
   Size:        687     459
 
EcoRI G'AATT_C

Cuts at:      0    1138    1146 
   Size:       1138       8
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     463     537    1146 
   Size:        463      74     609

  Fragments arranged by size: 

                 609     463      74
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     394     426    1146 
   Size:        394      32     720

  Fragments arranged by size: 

                 720     394      32
 
HincII GTy'rAC

Cuts at:      0    1087    1146 
   Size:       1087      59
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     122    1146 
   Size:        122    1024
 
MscI TGG'CCA

Cuts at:      0     328    1146 
   Size:        328     818
 
NsiI A_TGCA'T

Cuts at:      0     189    1146 
   Size:        189     957
 
NspI r_CATG'y

Cuts at:      0      70    1146 
   Size:         70    1076
 
PmlI CAC'GTG

Cuts at:      0     888    1146 
   Size:        888     258
 
SmlI C'TyrA_G

Cuts at:      0     413    1146 
   Size:        413     733
 
SphI G_CATG'C

Cuts at:      0      70    1146 
   Size:         70    1076
 
SspI AAT'ATT

Cuts at:      0     117     360    1146 
   Size:        117     243     786

  Fragments arranged by size: 

                 786     243     117
 
StyI C'CwwG_G

Cuts at:      0     377     628    1146 
   Size:        377     251     518

  Fragments arranged by size: 

                 518     377     251
 
TatI w'GTAC_w

Cuts at:      0    1109    1146 
   Size:       1109      37
 
XbaI T'CTAG_A

Cuts at:      0    1023    1146 
   Size:       1023     123
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     839    1146 
   Size:        839     307
 
XhoI C'TCGA_G

Cuts at:      0     413    1146 
   Size:        413     733
 

 Enzymes that do cut:

     AcuI    AlwNI     ApoI     AvaI    BfrBI    BsaAI    BseRI     BsgI
     BsmI    BsrDI   BstAPI     BtsI     EaeI     EarI  Eco57MI    EcoNI
    EcoRI     FalI    Hin4I   HincII     MmeI     MscI     NsiI     NspI
     PmlI     SmlI     SphI     SspI     StyI     TatI     XbaI     XcmI
     XhoI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI     ApaI    ApaLI     AscI
     AseI    AsiSI    AvrII     BaeI    BamHI     BanI    BanII     BbeI
     BbsI    BbvCI     BcgI    BciVI     BclI     BglI    BglII     BlpI
 Bme1580I    BmgBI     BmrI     BmtI     BplI     BpmI   Bpu10I    BpuEI
     BsaI    BsaBI    BsaHI    BsaWI    BsaXI    BseYI    BsiEI  BsiHKAI
    BsiWI    BsmBI Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrFI
    BsrGI   BssHII    BssSI    BstBI   BstEII    BstXI    BstYI  BstZ17I
   Bsu36I     BtgI    BtgZI     ClaI    CspCI     DraI   DraIII     DrdI
     EagI     EciI  EcoICRI EcoO109I    EcoRV     FseI     FspI    FspAI
    HaeII  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MslI
   MspA1I     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI
     NruI     PacI     PasI     PciI    PflMI     PfoI     PmeI     PpiI
    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI
    PvuII    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI
     ScaI    SexAI     SfcI     SfiI     SfoI     SgfI    SgrAI     SmaI
    SnaBI     SpeI     SrfI     StuI     SwaI    TaqII     TstI  Tth111I
     XmaI     XmnI     ZraI