Restriction map for
pF1KB7268
(Linear) (Six-base) MAPSORT of: KIBB7268.seq Check: 5955 from: 1 to: 1644
KIBB7268.orf
With 182 enzymes: SgfI *
October 7, 2009 14:23 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 174 1644
Size: 174 1470
AflIII A'CryG_T
Cuts at: 0 1524 1644
Size: 1524 120
AgeI A'CCGG_T
Cuts at: 0 1177 1644
Size: 1177 467
AhdI GACnn_n'nnGTC
Cuts at: 0 1472 1644
Size: 1472 172
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 47 1644
Size: 47 1597
ApoI r'AATT_y
Cuts at: 0 80 581 665 813 1001 1169 1253 1337
Size: 80 501 84 148 188 168 84 84
Cuts at: 1337 1588 1644
Size: 251 56
Fragments arranged by size:
501 251 188 168 148 84 84 84
80 56
AseI AT'TA_AT
Cuts at: 0 653 1644
Size: 653 991
BanI G'GyrC_C
Cuts at: 0 162 1644
Size: 162 1482
BanII G_rGCy'C
Cuts at: 0 284 1231 1644
Size: 284 947 413
Fragments arranged by size:
947 413 284
BbsI GAAGACnn'nnnn_
Cuts at: 0 126 1471 1644
Size: 126 1345 173
Fragments arranged by size:
1345 173 126
BclI T'GATC_A
Cuts at: 0 518 1644
Size: 518 1126
BfrBI ATG'CAT
Cuts at: 0 559 1027 1509 1644
Size: 559 468 482 135
Fragments arranged by size:
559 482 468 135
BmgBI CAC'GTC
Cuts at: 0 192 1644
Size: 192 1452
BmrI ACTGGGnnnn_n'
Cuts at: 0 235 1644
Size: 235 1409
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 329 1283 1644
Size: 329 954 361
Fragments arranged by size:
954 361 329
Bpu10I CC'TnA_GC
Cuts at: 0 9 1644
Size: 9 1635
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 451 1222 1491 1644
Size: 451 771 269 153
Fragments arranged by size:
771 451 269 153
BsaI GGTCTCn'nnnn_
Cuts at: 0 196 225 1317 1644
Size: 196 29 1092 327
Fragments arranged by size:
1092 327 196 29
BsaBI GATnn'nnATC
Cuts at: 0 520 1644
Size: 520 1124
BsaWI w'CCGG_w
Cuts at: 0 1177 1644
Size: 1177 467
BsiHKAI G_wGCw'C
Cuts at: 0 1231 1644
Size: 1231 413
BsmI GAATG_Cn'
Cuts at: 0 466 1511 1644
Size: 466 1045 133
Fragments arranged by size:
1045 466 133
BsmBI CGTCTCn'nnnn_
Cuts at: 0 182 1644
Size: 182 1462
Bsp1286I G_dGCh'C
Cuts at: 0 284 1231 1644
Size: 284 947 413
Fragments arranged by size:
947 413 284
BspHI T'CATG_A
Cuts at: 0 1476 1644
Size: 1476 168
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 174 1644
Size: 174 1470
BsrDI GCAATG_nn'
Cuts at: 0 1392 1644
Size: 1392 252
BsrFI r'CCGG_y
Cuts at: 0 1177 1644
Size: 1177 467
BsrGI T'GTAC_A
Cuts at: 0 182 1326 1644
Size: 182 1144 318
Fragments arranged by size:
1144 318 182
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1615 1644
Size: 1615 29
BstYI r'GATC_y
Cuts at: 0 974 1644
Size: 974 670
BtsI GCAGTG_nn'
Cuts at: 0 160 1644
Size: 160 1484
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1020 1055 1188 1223 1644
Size: 1020 35 133 35 421
Fragments arranged by size:
1020 421 133 35 35
DraI TTT'AAA
Cuts at: 0 717 1644
Size: 717 927
EaeI y'GGCC_r
Cuts at: 0 125 1644
Size: 125 1519
EcoICRI GAG'CTC
Cuts at: 0 1229 1644
Size: 1229 415
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 329 1283 1644
Size: 329 954 361
Fragments arranged by size:
954 361 329
EcoO109I rG'GnC_Cy
Cuts at: 0 63 1298 1644
Size: 63 1235 346
Fragments arranged by size:
1235 346 63
EcoRI G'AATT_C
Cuts at: 0 1001 1169 1253 1337 1644
Size: 1001 168 84 84 307
Fragments arranged by size:
1001 307 168 84 84
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 52 84 241 273 894 926 966 998
Size: 52 32 157 32 621 32 40 32
Cuts at: 998 1062 1094 1398 1430 1458 1490 1644
Size: 64 32 304 32 28 32 154
Fragments arranged by size:
621 304 157 154 64 52 40 32
32 32 32 32 32 32 28
MfeI C'AATT_G
Cuts at: 0 1402 1644
Size: 1402 242
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 179 1644
Size: 179 1465
MscI TGG'CCA
Cuts at: 0 127 1644
Size: 127 1517
MslI CAynn'nnrTG
Cuts at: 0 374 393 774 842 1010 1178 1346 1362
Size: 374 19 381 68 168 168 168 16
Cuts at: 1362 1613 1644
Size: 251 31
Fragments arranged by size:
381 374 251 168 168 168 68 31
19 16
NdeI CA'TA_TG
Cuts at: 0 771 1359 1610 1644
Size: 771 588 251 34
Fragments arranged by size:
771 588 251 34
NsiI A_TGCA'T
Cuts at: 0 561 1029 1511 1644
Size: 561 468 482 133
Fragments arranged by size:
561 482 468 133
NspI r_CATG'y
Cuts at: 0 373 559 1031 1528 1644
Size: 373 186 472 497 116
Fragments arranged by size:
497 472 373 186 116
PciI A'CATG_T
Cuts at: 0 1524 1644
Size: 1524 120
PpuMI rG'GwC_Cy
Cuts at: 0 63 1644
Size: 63 1581
SacI G_AGCT'C
Cuts at: 0 1231 1644
Size: 1231 413
SexAI A'CCwGG_T
Cuts at: 0 215 1644
Size: 215 1429
SfcI C'TryA_G
Cuts at: 0 207 1644
Size: 207 1437
SmlI C'TyrA_G
Cuts at: 0 430 1237 1470 1644
Size: 430 807 233 174
Fragments arranged by size:
807 430 233 174
SphI G_CATG'C
Cuts at: 0 559 1031 1644
Size: 559 472 613
Fragments arranged by size:
613 559 472
SspI AAT'ATT
Cuts at: 0 750 1644
Size: 750 894
StuI AGG'CCT
Cuts at: 0 314 712 796 880 964 1216 1644
Size: 314 398 84 84 84 252 428
Fragments arranged by size:
428 398 314 252 84 84 84
StyI C'CwwG_G
Cuts at: 0 99 347 1644
Size: 99 248 1297
Fragments arranged by size:
1297 248 99
TatI w'GTAC_w
Cuts at: 0 182 990 1326 1644
Size: 182 808 336 318
Fragments arranged by size:
808 336 318 182
XmnI GAAnn'nnTTC
Cuts at: 0 356 1644
Size: 356 1288
Enzymes that do cut:
AarI AflIII AgeI AhdI AlfI ApoI AseI BanI
BanII BbsI BclI BfrBI BmgBI BmrI BpmI Bpu10I
BpuEI BsaI BsaBI BsaWI BsiHKAI BsmI BsmBI Bsp1286I
BspHI BspMI BsrDI BsrFI BsrGI BstXI BstYI BtsI
CspCI DraI EaeI EcoICRI Eco57MI EcoO109I EcoRI Hin4I
MfeI MmeI MscI MslI NdeI NsiI NspI PciI
PpuMI SacI SexAI SfcI SmlI SphI SspI StuI
StyI TatI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AleI
AloI AlwNI ApaI ApaLI AscI AsiSI AvaI AvrII
BaeI BamHI BbeI BbvCI BcgI BciVI BglI BglII
BlpI Bme1580I BmtI BplI BsaAI BsaHI BsaXI BseRI
BseYI BsgI BsiEI BsiWI BspEI BsrBI BssHII BssSI
BstAPI BstBI BstEII BstZ17I Bsu36I BtgI BtgZI ClaI
DraIII DrdI EagI EarI EciI EcoNI EcoRV FalI
FseI FspI FspAI HaeII HincII HindIII HpaI KasI
KpnI MluI MspA1I NaeI NarI NcoI NgoMIV NheI
NotI NruI PacI PasI PflMI PfoI PmeI PmlI
PpiI PshAI PsiI PspOMI PspXI PsrI PstI PvuI
PvuII RsrII SacII SalI SanDI SapI SbfI ScaI
SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SrfI
SwaI TaqII TstI Tth111I XbaI XcmI XhoI XmaI
ZraI