Restriction map for
pF1KB7223
(Linear) (Six-base) MAPSORT of: KIBB7223.seq Check: 5662 from: 1 to: 1515
KIBB7223.orf
With 182 enzymes: SgfI *
October 7, 2009 14:23 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 474 1026 1459 1515
Size: 474 552 433 56
Fragments arranged by size:
552 474 433 56
AflIII A'CryG_T
Cuts at: 0 594 1515
Size: 594 921
AlwNI CAG_nnn'CTG
Cuts at: 0 361 1515
Size: 361 1154
ApoI r'AATT_y
Cuts at: 0 176 370 502 915 1075 1515
Size: 176 194 132 413 160 440
Fragments arranged by size:
440 413 194 176 160 132
BanI G'GyrC_C
Cuts at: 0 786 1515
Size: 786 729
BanII G_rGCy'C
Cuts at: 0 214 1515
Size: 214 1301
BbsI GAAGACnn'nnnn_
Cuts at: 0 591 865 1515
Size: 591 274 650
Fragments arranged by size:
650 591 274
BciVI GTATCCnnnnn_n'
Cuts at: 0 140 414 1515
Size: 140 274 1101
Fragments arranged by size:
1101 274 140
BclI T'GATC_A
Cuts at: 0 1370 1515
Size: 1370 145
BfrBI ATG'CAT
Cuts at: 0 1424 1515
Size: 1424 91
BglII A'GATC_T
Cuts at: 0 236 1329 1515
Size: 236 1093 186
Fragments arranged by size:
1093 236 186
BmrI ACTGGGnnnn_n'
Cuts at: 0 1452 1515
Size: 1452 63
BsaBI GATnn'nnATC
Cuts at: 0 1375 1515
Size: 1375 140
BsaWI w'CCGG_w
Cuts at: 0 167 1515
Size: 167 1348
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1457 1515
Size: 1457 58
BseYI C'CCAG_C
Cuts at: 0 318 729 751 962 1289 1344 1515
Size: 318 411 22 211 327 55 171
Fragments arranged by size:
411 327 318 211 171 55 22
BsiHKAI G_wGCw'C
Cuts at: 0 191 1515
Size: 191 1324
BsmI GAATG_Cn'
Cuts at: 0 715 833 895 958 1515
Size: 715 118 62 63 557
Fragments arranged by size:
715 557 118 63 62
Bsp1286I G_dGCh'C
Cuts at: 0 191 214 1515
Size: 191 23 1301
Fragments arranged by size:
1301 191 23
BsrDI GCAATG_nn'
Cuts at: 0 985 1515
Size: 985 530
BsrGI T'GTAC_A
Cuts at: 0 1090 1515
Size: 1090 425
BstEII G'GTnAC_C
Cuts at: 0 840 1515
Size: 840 675
BstYI r'GATC_y
Cuts at: 0 236 1329 1515
Size: 236 1093 186
Fragments arranged by size:
1093 236 186
BtsI GCAGTG_nn'
Cuts at: 0 79 1515
Size: 79 1436
EaeI y'GGCC_r
Cuts at: 0 268 1148 1515
Size: 268 880 367
Fragments arranged by size:
880 367 268
EarI CTCTTCn'nnn_
Cuts at: 0 672 700 1515
Size: 672 28 815
Fragments arranged by size:
815 672 28
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 838 1515
Size: 838 677
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 474 1026 1459 1515
Size: 474 552 433 56
Fragments arranged by size:
552 474 433 56
EcoRV GAT'ATC
Cuts at: 0 1445 1515
Size: 1445 70
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 852 884 1515
Size: 852 32 631
Fragments arranged by size:
852 631 32
HindIII A'AGCT_T
Cuts at: 0 58 1184 1515
Size: 58 1126 331
Fragments arranged by size:
1126 331 58
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 527 1515
Size: 527 988
MscI TGG'CCA
Cuts at: 0 270 1515
Size: 270 1245
MspA1I CmG'CkG
Cuts at: 0 322 1046 1344 1515
Size: 322 724 298 171
Fragments arranged by size:
724 322 298 171
NsiI A_TGCA'T
Cuts at: 0 1426 1515
Size: 1426 89
NspI r_CATG'y
Cuts at: 0 598 1515
Size: 598 917
PciI A'CATG_T
Cuts at: 0 594 1515
Size: 594 921
PflMI CCAn_nnn'nTGG
Cuts at: 0 736 1515
Size: 736 779
PfoI T'CCnGG_A
Cuts at: 0 1005 1515
Size: 1005 510
PstI C_TGCA'G
Cuts at: 0 914 1515
Size: 914 601
PvuII CAG'CTG
Cuts at: 0 322 1046 1344 1515
Size: 322 724 298 171
Fragments arranged by size:
724 322 298 171
ScaI AGT'ACT
Cuts at: 0 1039 1515
Size: 1039 476
SfcI C'TryA_G
Cuts at: 0 362 910 927 1263 1515
Size: 362 548 17 336 252
Fragments arranged by size:
548 362 336 252 17
SspI AAT'ATT
Cuts at: 0 156 1515
Size: 156 1359
StyI C'CwwG_G
Cuts at: 0 735 880 1515
Size: 735 145 635
Fragments arranged by size:
735 635 145
TatI w'GTAC_w
Cuts at: 0 1037 1059 1090 1515
Size: 1037 22 31 425
Fragments arranged by size:
1037 425 31 22
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 388 420 1008 1040 1515
Size: 388 32 588 32 475
Fragments arranged by size:
588 475 388 32 32
XbaI T'CTAG_A
Cuts at: 0 451 1515
Size: 451 1064
XmnI GAAnn'nnTTC
Cuts at: 0 346 1515
Size: 346 1169
Enzymes that do cut:
AcuI AflIII AlwNI ApoI BanI BanII BbsI BciVI
BclI BfrBI BglII BmrI BsaBI BsaWI BseRI BseYI
BsiHKAI BsmI Bsp1286I BsrDI BsrGI BstEII BstYI BtsI
EaeI EarI EciI Eco57MI EcoRV Hin4I HindIII MmeI
MscI MspA1I NsiI NspI PciI PflMI PfoI PstI
PvuII ScaI SfcI SspI StyI TatI TstI XbaI
XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AhdI AleI AlfI AloI ApaI ApaLI AscI AseI
AsiSI AvaI AvrII BaeI BamHI BbeI BbvCI BcgI
BglI BlpI Bme1580I BmgBI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaHI BsaXI BsgI BsiEI BsiWI
BsmBI BspEI BspHI BspMI BsrBI BsrFI BssHII BssSI
BstAPI BstBI BstXI BstZ17I Bsu36I BtgI BtgZI ClaI
CspCI DraI DraIII DrdI EagI EcoICRI EcoNI EcoO109I
EcoRI FalI FseI FspI FspAI HaeII HincII HpaI
KasI KpnI MfeI MluI MslI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI PacI PasI PmeI
PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI
PvuI RsrII SacI SacII SalI SanDI SapI SbfI
SexAI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SrfI StuI SwaI TaqII Tth111I XcmI
XhoI XmaI ZraI