(Linear) (Six-base) MAPSORT of: KIBB7221.seq  Check: 2409  from: 1  to: 621



KIBB7221.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 10:59  ..

AccI GT'mk_AC

Cuts at:      0     177     621 
   Size:        177     444
 
AclI AA'CG_TT

Cuts at:      0     101     621 
   Size:        101     520
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     276     621 
   Size:        276     345
 
AlwNI CAG_nnn'CTG

Cuts at:      0     539     621 
   Size:        539      82
 
ApoI r'AATT_y

Cuts at:      0     595     621 
   Size:        595      26
 
BanI G'GyrC_C

Cuts at:      0      70     621 
   Size:         70     551
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     398     416     621 
   Size:        398      18     205

  Fragments arranged by size: 

                 398     205      18
 
BclI T'GATC_A

Cuts at:      0     354     621 
   Size:        354     267
 
BlpI GC'TnA_GC

Cuts at:      0     469     621 
   Size:        469     152
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      61     621 
   Size:         61     560
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     501     621 
   Size:        501     120
 
BseYI C'CCAG_C

Cuts at:      0     464     621 
   Size:        464     157
 
BsiHKAI G_wGCw'C

Cuts at:      0       7     621 
   Size:          7     614
 
Bsp1286I G_dGCh'C

Cuts at:      0       7     621 
   Size:          7     614
 
BsrDI GCAATG_nn'

Cuts at:      0      62     621 
   Size:         62     559
 
BstYI r'GATC_y

Cuts at:      0      44     621 
   Size:         44     577
 
EarI CTCTTCn'nnn_

Cuts at:      0     108     232     621 
   Size:        108     124     389

  Fragments arranged by size: 

                 389     124     108
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     276     621 
   Size:        276     345
 
EcoO109I rG'GnC_Cy

Cuts at:      0     459     621 
   Size:        459     162
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     273     621 
   Size:        273     348
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     551     583     621 
   Size:        551      32      38

  Fragments arranged by size: 

                 551      38      32
 
MspA1I CmG'CkG

Cuts at:      0     468     621 
   Size:        468     153
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     384     621 
   Size:        384     237
 
PpuMI rG'GwC_Cy

Cuts at:      0     459     621 
   Size:        459     162
 
PvuII CAG'CTG

Cuts at:      0     468     621 
   Size:        468     153
 
SanDI GG'GwC_CC

Cuts at:      0     459     621 
   Size:        459     162
 
SfcI C'TryA_G

Cuts at:      0     167     274     621 
   Size:        167     107     347

  Fragments arranged by size: 

                 347     167     107
 
SmlI C'TyrA_G

Cuts at:      0      40     621 
   Size:         40     581
 
SspI AAT'ATT

Cuts at:      0     313     621 
   Size:        313     308
 
StuI AGG'CCT

Cuts at:      0     256     280     621 
   Size:        256      24     341

  Fragments arranged by size: 

                 341     256      24
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     241     621 
   Size:        241     380
 
XmnI GAAnn'nnTTC

Cuts at:      0     256     621 
   Size:        256     365
 

 Enzymes that do cut:

     AccI     AclI     AcuI    AlwNI     ApoI     BanI     BbsI     BclI
     BlpI    BpuEI    BseRI    BseYI  BsiHKAI Bsp1286I    BsrDI    BstYI
     EarI  Eco57MI EcoO109I     FalI    Hin4I   MspA1I    PflMI    PpuMI
    PvuII    SanDI     SfcI     SmlI     SspI     StuI    TaqII     XmnI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AfeI    AflII   AflIII     AgeI     AhdI
     AleI     AlfI     AloI     ApaI    ApaLI     AscI     AseI    AsiSI
     AvaI    AvrII     BaeI    BamHI    BanII     BbeI    BbvCI     BcgI
    BciVI    BfrBI     BglI    BglII Bme1580I    BmgBI     BmrI     BmtI
     BplI     BpmI   Bpu10I     BsaI    BsaAI    BsaBI    BsaHI    BsaWI
    BsaXI     BsgI    BsiEI    BsiWI     BsmI    BsmBI    BspEI    BspHI
    BspMI    BsrBI    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI
   BstEII    BstXI  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI     ClaI
    CspCI     DraI   DraIII     DrdI     EaeI     EagI     EciI  EcoICRI
    EcoNI    EcoRI    EcoRV     FseI     FspI    FspAI    HaeII   HincII
  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MmeI     MscI
     MslI     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI
     NruI     NsiI     NspI     PacI     PasI     PciI     PfoI     PmeI
     PmlI     PpiI    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI
     PvuI    RsrII     SacI    SacII     SalI     SapI     SbfI     ScaI
    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI
     SphI     SrfI     StyI     SwaI     TatI     TstI  Tth111I     XbaI
     XcmI     XhoI     XmaI     ZraI