Restriction map for
pF1KB7217
(Linear) (Six-base) MAPSORT of: KIBB7217.seq Check: 5037 from: 1 to: 978
KIBB7217.orf
With 182 enzymes: SgfI *
March 2, 2010 14:27 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 782 978
Size: 782 196
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 82 295 978
Size: 82 213 683
Fragments arranged by size:
683 213 82
AflIII A'CryG_T
Cuts at: 0 874 887 978
Size: 874 13 91
Fragments arranged by size:
874 91 13
AleI CACnn'nnGTG
Cuts at: 0 875 978
Size: 875 103
ApoI r'AATT_y
Cuts at: 0 378 924 978
Size: 378 546 54
Fragments arranged by size:
546 378 54
BbsI GAAGACnn'nnnn_
Cuts at: 0 457 978
Size: 457 521
BclI T'GATC_A
Cuts at: 0 384 978
Size: 384 594
BsaI GGTCTCn'nnnn_
Cuts at: 0 164 978
Size: 164 814
BsaBI GATnn'nnATC
Cuts at: 0 495 978
Size: 495 483
BsaWI w'CCGG_w
Cuts at: 0 155 978
Size: 155 823
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 152 155 978
Size: 152 3 823
Fragments arranged by size:
823 152 3
BsiHKAI G_wGCw'C
Cuts at: 0 309 978
Size: 309 669
BsmI GAATG_Cn'
Cuts at: 0 23 978
Size: 23 955
Bsp1286I G_dGCh'C
Cuts at: 0 309 978
Size: 309 669
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 782 978
Size: 782 196
BsrDI GCAATG_nn'
Cuts at: 0 127 978
Size: 127 851
BstBI TT'CG_AA
Cuts at: 0 426 978
Size: 426 552
BstXI CCAn_nnnn'nTGG
Cuts at: 0 446 978
Size: 446 532
BtgI C'CryG_G
Cuts at: 0 278 592 978
Size: 278 314 386
Fragments arranged by size:
386 314 278
BtsI GCAGTG_nn'
Cuts at: 0 784 807 978
Size: 784 23 171
Fragments arranged by size:
784 171 23
EaeI y'GGCC_r
Cuts at: 0 897 978
Size: 897 81
EarI CTCTTCn'nnn_
Cuts at: 0 245 314 978
Size: 245 69 664
Fragments arranged by size:
664 245 69
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 82 295 978
Size: 82 213 683
Fragments arranged by size:
683 213 82
EcoO109I rG'GnC_Cy
Cuts at: 0 7 978
Size: 7 971
EcoRI G'AATT_C
Cuts at: 0 378 978
Size: 378 600
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 142 174 978
Size: 142 32 804
Fragments arranged by size:
804 142 32
MscI TGG'CCA
Cuts at: 0 899 978
Size: 899 79
MslI CAynn'nnrTG
Cuts at: 0 239 353 742 875 978
Size: 239 114 389 133 103
Fragments arranged by size:
389 239 133 114 103
MspA1I CmG'CkG
Cuts at: 0 32 978
Size: 32 946
NcoI C'CATG_G
Cuts at: 0 278 592 978
Size: 278 314 386
Fragments arranged by size:
386 314 278
NspI r_CATG'y
Cuts at: 0 49 540 878 891 978
Size: 49 491 338 13 87
Fragments arranged by size:
491 338 87 49 13
PciI A'CATG_T
Cuts at: 0 874 887 978
Size: 874 13 91
Fragments arranged by size:
874 91 13
PpuMI rG'GwC_Cy
Cuts at: 0 7 978
Size: 7 971
PstI C_TGCA'G
Cuts at: 0 779 978
Size: 779 199
SapI GCTCTTCn'nnn_
Cuts at: 0 314 978
Size: 314 664
SfcI C'TryA_G
Cuts at: 0 696 775 978
Size: 696 79 203
Fragments arranged by size:
696 203 79
StyI C'CwwG_G
Cuts at: 0 278 592 978
Size: 278 314 386
Fragments arranged by size:
386 314 278
TatI w'GTAC_w
Cuts at: 0 316 395 781 978
Size: 316 79 386 197
Fragments arranged by size:
386 316 197 79
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 400 978
Size: 400 578
Enzymes that do cut:
AarI AcuI AflIII AleI ApoI BbsI BclI BsaI
BsaBI BsaWI BseRI BsiHKAI BsmI Bsp1286I BspMI BsrDI
BstBI BstXI BtgI BtsI EaeI EarI Eco57MI EcoO109I
EcoRI Hin4I MscI MslI MspA1I NcoI NspI PciI
PpuMI PstI SapI SfcI StyI TatI XcmI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AgeI AhdI
AlfI AloI AlwNI ApaI ApaLI AscI AseI AsiSI
AvaI AvrII BaeI BamHI BanI BanII BbeI BbvCI
BcgI BciVI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaAI BsaHI
BsaXI BseYI BsgI BsiEI BsiWI BsmBI BspEI BspHI
BsrBI BsrFI BsrGI BssHII BssSI BstAPI BstEII BstYI
BstZ17I Bsu36I BtgZI ClaI CspCI DraI DraIII DrdI
EagI EciI EcoICRI EcoNI EcoRV FalI FseI FspI
FspAI HaeII HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI NaeI NarI NdeI NgoMIV NheI NotI
NruI NsiI PacI PasI PflMI PfoI PmeI PmlI
PpiI PshAI PsiI PspOMI PspXI PsrI PvuI PvuII
RsrII SacI SacII SalI SanDI SbfI ScaI SexAI
SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SrfI SspI StuI SwaI TaqII TstI Tth111I
XbaI XhoI XmaI XmnI ZraI