(Linear) (Six-base) MAPSORT of: KIBB7208.seq  Check: 6562  from: 1  to: 318



KIBB7208.orf

 With 182 enzymes: SgfI * 

                        June 26, 2008 10:49  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     169     318 
   Size:        169     149
 
AflIII A'CryG_T

Cuts at:      0      28     318 
   Size:         28     290
 
AlwNI CAG_nnn'CTG

Cuts at:      0      81     318 
   Size:         81     237
 
AvaI C'yCGr_G

Cuts at:      0     228     318 
   Size:        228      90
 
BsaAI yAC'GTr

Cuts at:      0      31     318 
   Size:         31     287
 
BseYI C'CCAG_C

Cuts at:      0     256     318 
   Size:        256      62
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      53     318 
   Size:         53     265
 
BspHI T'CATG_A

Cuts at:      0      98     318 
   Size:         98     220
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     150     318 
   Size:        150     168
 
EarI CTCTTCn'nnn_

Cuts at:      0     248     318 
   Size:        248      70
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     169     318 
   Size:        169     149
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     259     318 
   Size:        259      59
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      34      66     318 
   Size:         34      32     252

  Fragments arranged by size: 

                 252      34      32
 
MslI CAynn'nnrTG

Cuts at:      0     265     318 
   Size:        265      53
 
PmlI CAC'GTG

Cuts at:      0      31     318 
   Size:         31     287
 
PshAI GACnn'nnGTC

Cuts at:      0      10     318 
   Size:         10     308
 
SmaI CCC'GGG

Cuts at:      0     230     318 
   Size:        230      88
 
SspI AAT'ATT

Cuts at:      0     138     318 
   Size:        138     180
 
XmaI C'CCGG_G

Cuts at:      0     228     318 
   Size:        228      90
 

 Enzymes that do cut:

     AcuI   AflIII    AlwNI     AvaI    BsaAI    BseYI     BsgI    BspHI
   BstAPI     EarI  Eco57MI     FalI    Hin4I     MslI     PmlI    PshAI
     SmaI     SspI     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII     AgeI
     AhdI     AleI     AlfI     AloI     ApaI    ApaLI     ApoI     AscI
     AseI    AsiSI    AvrII     BaeI    BamHI     BanI    BanII     BbeI
     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI    BglII
     BlpI Bme1580I    BmgBI     BmrI     BmtI     BplI     BpmI   Bpu10I
    BpuEI     BsaI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BsiEI
  BsiHKAI    BsiWI     BsmI    BsmBI Bsp1286I    BspEI    BspMI    BsrBI
    BsrDI    BsrFI    BsrGI   BssHII    BssSI    BstBI   BstEII    BstXI
    BstYI  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI     ClaI    CspCI
     DraI   DraIII     DrdI     EaeI     EagI     EciI  EcoICRI    EcoNI
 EcoO109I    EcoRI    EcoRV     FseI     FspI    FspAI    HaeII   HincII
  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MmeI     MscI
   MspA1I     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI
     NruI     NsiI     NspI     PacI     PasI     PciI    PflMI     PfoI
     PmeI     PpiI    PpuMI     PsiI   PspOMI    PspXI     PsrI     PstI
     PvuI    PvuII    RsrII     SacI    SacII     SalI    SanDI     SapI
     SbfI     ScaI    SexAI     SfcI     SfiI     SfoI     SgfI    SgrAI
     SmlI    SnaBI     SpeI     SphI     SrfI     StuI     StyI     SwaI
    TaqII     TatI     TstI  Tth111I     XbaI     XcmI     XhoI     XmnI
     ZraI