(Linear) (Six-base) MAPSORT of: KIBB7204.seq  Check: 1205  from: 1  to: 567



KIBB7204.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 10:59  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     178     255     567 
   Size:        178      77     312

  Fragments arranged by size: 

                 312     178      77
 
AlwNI CAG_nnn'CTG

Cuts at:      0     345     567 
   Size:        345     222
 
ApoI r'AATT_y

Cuts at:      0     319     567 
   Size:        319     248
 
BlpI GC'TnA_GC

Cuts at:      0      40     567 
   Size:         40     527
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     206     567 
   Size:        206     361
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     298     567 
   Size:        298     269
 
BsiHKAI G_wGCw'C

Cuts at:      0      41     567 
   Size:         41     526
 
Bsp1286I G_dGCh'C

Cuts at:      0      41     567 
   Size:         41     526
 
BspHI T'CATG_A

Cuts at:      0     512     567 
   Size:        512      55
 
BstYI r'GATC_y

Cuts at:      0     517     567 
   Size:        517      50
 
EaeI y'GGCC_r

Cuts at:      0      26     567 
   Size:         26     541
 
EarI CTCTTCn'nnn_

Cuts at:      0     274     383     567 
   Size:        274     109     184

  Fragments arranged by size: 

                 274     184     109
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     178     255     567 
   Size:        178      77     312

  Fragments arranged by size: 

                 312     178      77
 
EcoO109I rG'GnC_Cy

Cuts at:      0     107     567 
   Size:        107     460
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     249     281     494     526     567 
   Size:        249      32     213      32      41

  Fragments arranged by size: 

                 249     213      41      32      32
 
SmlI C'TyrA_G

Cuts at:      0     221     567 
   Size:        221     346
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0      60     567 
   Size:         60     507
 

 Enzymes that do cut:

     AcuI    AlwNI     ApoI     BlpI    BpuEI     BsaI  BsiHKAI Bsp1286I
    BspHI    BstYI     EaeI     EarI  Eco57MI EcoO109I    Hin4I     SmlI
     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI     ApaI    ApaLI     AscI
     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI    BanII
     BbeI     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI
    BglII Bme1580I    BmgBI     BmrI     BmtI     BplI     BpmI   Bpu10I
    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI
    BsiEI    BsiWI     BsmI    BsmBI    BspEI    BspMI    BsrBI    BsrDI
    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI
  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI     ClaI    CspCI     DraI
   DraIII     DrdI     EagI     EciI  EcoICRI    EcoNI    EcoRI    EcoRV
     FalI     FseI     FspI    FspAI    HaeII   HincII  HindIII     HpaI
     KasI     KpnI     MfeI     MluI     MmeI     MscI     MslI   MspA1I
     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI
     NsiI     NspI     PacI     PasI     PciI    PflMI     PfoI     PmeI
     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI
     PstI     PvuI    PvuII    RsrII     SacI    SacII     SalI    SanDI
     SapI     SbfI     ScaI    SexAI     SfcI     SfiI     SfoI     SgfI
    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI     SspI     StuI
     StyI     SwaI    TaqII     TatI     TstI  Tth111I     XbaI     XhoI
     XmaI     XmnI     ZraI