Restriction map for
pF1KB7197
(Linear) (Six-base) MAPSORT of: KIBB7197.seq Check: 3031 from: 1 to: 1116
KIBB7197.orf
With 182 enzymes: SgfI *
March 3, 2008 10:59 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1017 1116
Size: 1017 99
AfeI AGC'GCT
Cuts at: 0 87 979 1116
Size: 87 892 137
Fragments arranged by size:
892 137 87
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 385 606 1116
Size: 385 221 510
Fragments arranged by size:
510 385 221
AvaI C'yCGr_G
Cuts at: 0 572 1116
Size: 572 544
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 495 528 1116
Size: 495 33 588
Fragments arranged by size:
588 495 33
BanI G'GyrC_C
Cuts at: 0 22 91 669 802 822 1116
Size: 22 69 578 133 20 294
Fragments arranged by size:
578 294 133 69 22 20
BanII G_rGCy'C
Cuts at: 0 745 1116
Size: 745 371
BbeI G_GCGC'C
Cuts at: 0 26 95 806 826 1116
Size: 26 69 711 20 290
Fragments arranged by size:
711 290 69 26 20
BbsI GAAGACnn'nnnn_
Cuts at: 0 247 1116
Size: 247 869
BclI T'GATC_A
Cuts at: 0 500 1116
Size: 500 616
BglII A'GATC_T
Cuts at: 0 641 1116
Size: 641 475
Bme1580I G_kGCm'C
Cuts at: 0 545 674 1116
Size: 545 129 442
Fragments arranged by size:
545 442 129
BmgBI CAC'GTC
Cuts at: 0 338 1116
Size: 338 778
BmrI ACTGGGnnnn_n'
Cuts at: 0 633 1116
Size: 633 483
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 583 1116
Size: 583 533
Bpu10I CC'TnA_GC
Cuts at: 0 276 1116
Size: 276 840
BsaBI GATnn'nnATC
Cuts at: 0 505 1116
Size: 505 611
BsaHI Gr'CG_yC
Cuts at: 0 23 62 92 803 823 1116
Size: 23 39 30 711 20 293
Fragments arranged by size:
711 293 39 30 23 20
BsaWI w'CCGG_w
Cuts at: 0 224 1116
Size: 224 892
BseYI C'CCAG_C
Cuts at: 0 82 192 607 613 915 1024 1116
Size: 82 110 415 6 302 109 92
Fragments arranged by size:
415 302 110 109 92 82 6
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 883 1062 1116
Size: 883 179 54
Fragments arranged by size:
883 179 54
BsiEI CG_ry'CG
Cuts at: 0 173 870 1014 1116
Size: 173 697 144 102
Fragments arranged by size:
697 173 144 102
BsiHKAI G_wGCw'C
Cuts at: 0 374 950 1116
Size: 374 576 166
Fragments arranged by size:
576 374 166
BsmBI CGTCTCn'nnnn_
Cuts at: 0 328 1116
Size: 328 788
Bsp1286I G_dGCh'C
Cuts at: 0 374 545 674 745 950 1116
Size: 374 171 129 71 205 166
Fragments arranged by size:
374 205 171 166 129 71
BsrBI CCG'CTC
Cuts at: 0 42 1116
Size: 42 1074
BsrFI r'CCGG_y
Cuts at: 0 6 19 869 1116
Size: 6 13 850 247
Fragments arranged by size:
850 247 13 6
BssHII G'CGCG_C
Cuts at: 0 116 1116
Size: 116 1000
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1007 1116
Size: 1007 109
BstEII G'GTnAC_C
Cuts at: 0 633 1116
Size: 633 483
BstYI r'GATC_y
Cuts at: 0 641 1116
Size: 641 475
Bsu36I CC'TnA_GG
Cuts at: 0 528 1116
Size: 528 588
BtgI C'CryG_G
Cuts at: 0 182 1052 1116
Size: 182 870 64
Fragments arranged by size:
870 182 64
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 426 894 1116
Size: 426 468 222
Fragments arranged by size:
468 426 222
BtsI GCAGTG_nn'
Cuts at: 0 590 729 1116
Size: 590 139 387
Fragments arranged by size:
590 387 139
DraIII CAC_nnn'GTG
Cuts at: 0 692 1116
Size: 692 424
EaeI y'GGCC_r
Cuts at: 0 170 245 290 1011 1116
Size: 170 75 45 721 105
Fragments arranged by size:
721 170 105 75 45
EagI C'GGCC_G
Cuts at: 0 170 1011 1116
Size: 170 841 105
Fragments arranged by size:
841 170 105
EarI CTCTTCn'nnn_
Cuts at: 0 49 661 1116
Size: 49 612 455
Fragments arranged by size:
612 455 49
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 6 976 1116
Size: 6 970 140
Fragments arranged by size:
970 140 6
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 583 1017 1116
Size: 583 434 99
Fragments arranged by size:
583 434 99
EcoO109I rG'GnC_Cy
Cuts at: 0 465 519 1116
Size: 465 54 597
Fragments arranged by size:
597 465 54
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 999 1116
Size: 999 117
FspI TGC'GCA
Cuts at: 0 721 1116
Size: 721 395
HaeII r_GCGC'y
Cuts at: 0 26 77 89 95 128 806 826 981
Size: 26 51 12 6 33 678 20 155
Cuts at: 981 1109 1116
Size: 128 7
Fragments arranged by size:
678 155 128 51 33 26 20 12
7 6
HincII GTy'rAC
Cuts at: 0 432 1116
Size: 432 684
KasI G'GCGC_C
Cuts at: 0 22 91 802 822 1116
Size: 22 69 711 20 294
Fragments arranged by size:
711 294 69 22 20
MscI TGG'CCA
Cuts at: 0 292 1116
Size: 292 824
MslI CAynn'nnrTG
Cuts at: 0 235 421 553 559 688 781 1116
Size: 235 186 132 6 129 93 335
Fragments arranged by size:
335 235 186 132 129 93 6
MspA1I CmG'CkG
Cuts at: 0 617 882 1116
Size: 617 265 234
Fragments arranged by size:
617 265 234
NaeI GCC'GGC
Cuts at: 0 21 1116
Size: 21 1095
NarI GG'CG_CC
Cuts at: 0 23 92 803 823 1116
Size: 23 69 711 20 293
Fragments arranged by size:
711 293 69 23 20
NgoMIV G'CCGG_C
Cuts at: 0 19 1116
Size: 19 1097
NotI GC'GGCC_GC
Cuts at: 0 1011 1116
Size: 1011 105
NspI r_CATG'y
Cuts at: 0 597 1116
Size: 597 519
PpuMI rG'GwC_Cy
Cuts at: 0 519 1116
Size: 519 597
PstI C_TGCA'G
Cuts at: 0 35 1116
Size: 35 1081
PvuII CAG'CTG
Cuts at: 0 617 1116
Size: 617 499
SapI GCTCTTCn'nnn_
Cuts at: 0 49 1116
Size: 49 1067
SfcI C'TryA_G
Cuts at: 0 31 1116
Size: 31 1085
SfoI GGC'GCC
Cuts at: 0 24 93 804 824 1116
Size: 24 69 711 20 292
Fragments arranged by size:
711 292 69 24 20
SgrAI Cr'CCGG_yG
Cuts at: 0 19 1116
Size: 19 1097
SmaI CCC'GGG
Cuts at: 0 574 1116
Size: 574 542
SphI G_CATG'C
Cuts at: 0 597 1116
Size: 597 519
StyI C'CwwG_G
Cuts at: 0 488 746 1116
Size: 488 258 370
Fragments arranged by size:
488 370 258
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 327 552 1116
Size: 327 225 564
Fragments arranged by size:
564 327 225
XmaI C'CCGG_G
Cuts at: 0 572 1116
Size: 572 544
Enzymes that do cut:
AcuI AfeI AlfI AvaI BaeI BanI BanII BbeI
BbsI BclI BglII Bme1580I BmgBI BmrI BpmI Bpu10I
BsaBI BsaHI BsaWI BseYI BsgI BsiEI BsiHKAI BsmBI
Bsp1286I BsrBI BsrFI BssHII BstAPI BstEII BstYI Bsu36I
BtgI BtgZI BtsI DraIII EaeI EagI EarI EciI
Eco57MI EcoO109I FalI FspI HaeII HincII KasI MscI
MslI MspA1I NaeI NarI NgoMIV NotI NspI PpuMI
PstI PvuII SapI SfcI SfoI SgrAI SmaI SphI
StyI XcmI XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AflII AflIII AgeI
AhdI AleI AloI AlwNI ApaI ApaLI ApoI AscI
AseI AsiSI AvrII BamHI BbvCI BcgI BciVI BfrBI
BglI BlpI BmtI BplI BpuEI BsaI BsaAI BsaXI
BseRI BsiWI BsmI BspEI BspHI BspMI BsrDI BsrGI
BssSI BstBI BstXI BstZ17I ClaI CspCI DraI DrdI
EcoICRI EcoNI EcoRI EcoRV FseI FspAI Hin4I HindIII
HpaI KpnI MfeI MluI MmeI NcoI NdeI NheI
NruI NsiI PacI PasI PciI PflMI PfoI PmeI
PmlI PpiI PshAI PsiI PspOMI PspXI PsrI PvuI
RsrII SacI SacII SalI SanDI SbfI ScaI SexAI
SfiI SgfI SmlI SnaBI SpeI SrfI SspI StuI
SwaI TaqII TatI TstI Tth111I XbaI XhoI XmnI
ZraI