(Linear) (Six-base) MAPSORT of: KIBB7195.seq  Check: 7988  from: 1  to: 387



KIBB7195.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 10:59  ..

AccI GT'mk_AC

Cuts at:      0     224     274     387 
   Size:        224      50     113

  Fragments arranged by size: 

                 224     113      50
 
AflIII A'CryG_T

Cuts at:      0     202     387 
   Size:        202     185
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0      66     387 
   Size:         66     321
 
AlwNI CAG_nnn'CTG

Cuts at:      0     362     387 
   Size:        362      25
 
ApaI G_GGCC'C

Cuts at:      0     356     387 
   Size:        356      31
 
BanII G_rGCy'C

Cuts at:      0     356     387 
   Size:        356      31
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      10     387 
   Size:         10     377
 
Bme1580I G_kGCm'C

Cuts at:      0     245     356     387 
   Size:        245     111      31

  Fragments arranged by size: 

                 245     111      31
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     277     288     387 
   Size:        277      11      99

  Fragments arranged by size: 

                 277      99      11
 
BseYI C'CCAG_C

Cuts at:      0     177     355     387 
   Size:        177     178      32

  Fragments arranged by size: 

                 178     177      32
 
BsiHKAI G_wGCw'C

Cuts at:      0     255     387 
   Size:        255     132
 
Bsp1286I G_dGCh'C

Cuts at:      0     245     255     356     387 
   Size:        245      10     101      31

  Fragments arranged by size: 

                 245     101      31      10
 
BsrDI GCAATG_nn'

Cuts at:      0      80     387 
   Size:         80     307
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     162     387 
   Size:        162     225
 
BstYI r'GATC_y

Cuts at:      0     159     387 
   Size:        159     228
 
BstZ17I GTA'TAC

Cuts at:      0     225     275     387 
   Size:        225      50     112

  Fragments arranged by size: 

                 225     112      50
 
DraIII CAC_nnn'GTG

Cuts at:      0     134     296     387 
   Size:        134     162      91

  Fragments arranged by size: 

                 162     134      91
 
EaeI y'GGCC_r

Cuts at:      0     287     387 
   Size:        287     100
 
EcoO109I rG'GnC_Cy

Cuts at:      0     174     352     387 
   Size:        174     178      35

  Fragments arranged by size: 

                 178     174      35
 
MscI TGG'CCA

Cuts at:      0     289     387 
   Size:        289      98
 
NspI r_CATG'y

Cuts at:      0     206     387 
   Size:        206     181
 
PciI A'CATG_T

Cuts at:      0     202     387 
   Size:        202     185
 
PpuMI rG'GwC_Cy

Cuts at:      0     174     387 
   Size:        174     213
 
PspOMI G'GGCC_C

Cuts at:      0     352     387 
   Size:        352      35
 
StuI AGG'CCT

Cuts at:      0     347     387 
   Size:        347      40
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     307     387 
   Size:        307      80
 
TatI w'GTAC_w

Cuts at:      0     334     387 
   Size:        334      53
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     301     387 
   Size:        301      86
 

 Enzymes that do cut:

     AccI   AflIII     AlfI    AlwNI     ApaI    BanII     BbsI Bme1580I
     BmrI    BseYI  BsiHKAI Bsp1286I    BsrDI    BstXI    BstYI  BstZ17I
   DraIII     EaeI EcoO109I     MscI     NspI     PciI    PpuMI   PspOMI
     StuI    TaqII     TatI     XcmI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AcuI     AfeI    AflII     AgeI
     AhdI     AleI     AloI    ApaLI     ApoI     AscI     AseI    AsiSI
     AvaI    AvrII     BaeI    BamHI     BanI     BbeI    BbvCI     BcgI
    BciVI     BclI    BfrBI     BglI    BglII     BlpI    BmgBI     BmtI
     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI
    BsaWI    BsaXI    BseRI     BsgI    BsiEI    BsiWI     BsmI    BsmBI
    BspEI    BspHI    BspMI    BsrBI    BsrFI    BsrGI   BssHII    BssSI
   BstAPI    BstBI   BstEII   Bsu36I     BtgI    BtgZI     BtsI     ClaI
    CspCI     DraI     DrdI     EagI     EarI     EciI  EcoICRI  Eco57MI
    EcoNI    EcoRI    EcoRV     FalI     FseI     FspI    FspAI    HaeII
    Hin4I   HincII  HindIII     HpaI     KasI     KpnI     MfeI     MluI
     MmeI     MslI   MspA1I     NaeI     NarI     NcoI     NdeI   NgoMIV
     NheI     NotI     NruI     NsiI     PacI     PasI    PflMI     PfoI
     PmeI     PmlI     PpiI    PshAI     PsiI    PspXI     PsrI     PstI
     PvuI    PvuII    RsrII     SacI    SacII     SalI    SanDI     SapI
     SbfI     ScaI    SexAI     SfcI     SfiI     SfoI     SgfI    SgrAI
     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI     SspI     StyI
     SwaI     TstI  Tth111I     XbaI     XhoI     XmaI     XmnI     ZraI