Restriction map for
pF1KB7181
(Linear) (Six-base) MAPSORT of: KIBB7181.seq Check: 3455 from: 1 to: 1065
KIBB7181.orf
With 182 enzymes: SgfI *
March 3, 2008 10:59 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 20 1065
Size: 20 1045
AccI GT'mk_AC
Cuts at: 0 824 1065
Size: 824 241
AlwNI CAG_nnn'CTG
Cuts at: 0 126 1065
Size: 126 939
ApaI G_GGCC'C
Cuts at: 0 308 1065
Size: 308 757
ApaLI G'TGCA_C
Cuts at: 0 1051 1065
Size: 1051 14
AvaI C'yCGr_G
Cuts at: 0 16 755 1065
Size: 16 739 310
Fragments arranged by size:
739 310 16
BanI G'GyrC_C
Cuts at: 0 46 400 1065
Size: 46 354 665
Fragments arranged by size:
665 354 46
BanII G_rGCy'C
Cuts at: 0 8 118 308 857 1065
Size: 8 110 190 549 208
Fragments arranged by size:
549 208 190 110 8
BbsI GAAGACnn'nnnn_
Cuts at: 0 658 1065
Size: 658 407
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 168 202 1065
Size: 168 34 863
Fragments arranged by size:
863 168 34
BglI GCCn_nnn'nGGC
Cuts at: 0 367 1065
Size: 367 698
Bme1580I G_kGCm'C
Cuts at: 0 308 403 1055 1065
Size: 308 95 652 10
Fragments arranged by size:
652 308 95 10
BmgBI CAC'GTC
Cuts at: 0 1050 1065
Size: 1050 15
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 420 1065
Size: 420 645
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 685 1065
Size: 685 380
BsaI GGTCTCn'nnnn_
Cuts at: 0 763 1065
Size: 763 302
BsaWI w'CCGG_w
Cuts at: 0 890 1065
Size: 890 175
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 820 850 1065
Size: 820 30 215
Fragments arranged by size:
820 215 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 16 1065
Size: 16 1049
BseYI C'CCAG_C
Cuts at: 0 199 222 761 1065
Size: 199 23 539 304
Fragments arranged by size:
539 304 199 23
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 255 1065
Size: 255 810
BsiEI CG_ry'CG
Cuts at: 0 362 1065
Size: 362 703
BsiHKAI G_wGCw'C
Cuts at: 0 1055 1065
Size: 1055 10
BsmBI CGTCTCn'nnnn_
Cuts at: 0 859 1065
Size: 859 206
Bsp1286I G_dGCh'C
Cuts at: 0 8 118 308 403 857 1055 1065
Size: 8 110 190 95 454 198 10
Fragments arranged by size:
454 198 190 110 95 10 8
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 20 664 1065
Size: 20 644 401
Fragments arranged by size:
644 401 20
BsrBI CCG'CTC
Cuts at: 0 386 1065
Size: 386 679
BsrDI GCAATG_nn'
Cuts at: 0 906 1065
Size: 906 159
BsrFI r'CCGG_y
Cuts at: 0 361 1065
Size: 361 704
BssHII G'CGCG_C
Cuts at: 0 523 544 1065
Size: 523 21 521
Fragments arranged by size:
523 521 21
BssSI C'ACGA_G
Cuts at: 0 960 1065
Size: 960 105
BstXI CCAn_nnnn'nTGG
Cuts at: 0 77 1065
Size: 77 988
BtgI C'CryG_G
Cuts at: 0 624 806 912 1065
Size: 624 182 106 153
Fragments arranged by size:
624 182 153 106
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 336 1065
Size: 336 729
BtsI GCAGTG_nn'
Cuts at: 0 990 1065
Size: 990 75
ClaI AT'CG_AT
Cuts at: 0 920 1065
Size: 920 145
EaeI y'GGCC_r
Cuts at: 0 206 293 359 551 945 1065
Size: 206 87 66 192 394 120
Fragments arranged by size:
394 206 192 120 87 66
EagI C'GGCC_G
Cuts at: 0 359 1065
Size: 359 706
EarI CTCTTCn'nnn_
Cuts at: 0 799 1065
Size: 799 266
EcoNI CCTnn'n_nnAGG
Cuts at: 0 449 1065
Size: 449 616
EcoO109I rG'GnC_Cy
Cuts at: 0 54 816 1065
Size: 54 762 249
Fragments arranged by size:
762 249 54
HaeII r_GCGC'y
Cuts at: 0 722 1065
Size: 722 343
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 532 564 706 738 1065
Size: 532 32 142 32 327
Fragments arranged by size:
532 327 142 32 32
MscI TGG'CCA
Cuts at: 0 295 1065
Size: 295 770
MspA1I CmG'CkG
Cuts at: 0 263 641 1022 1065
Size: 263 378 381 43
Fragments arranged by size:
381 378 263 43
NaeI GCC'GGC
Cuts at: 0 363 1065
Size: 363 702
NgoMIV G'CCGG_C
Cuts at: 0 361 1065
Size: 361 704
NspI r_CATG'y
Cuts at: 0 606 1065
Size: 606 459
PasI CC'CwG_GG
Cuts at: 0 90 1065
Size: 90 975
PfoI T'CCnGG_A
Cuts at: 0 230 593 1065
Size: 230 363 472
Fragments arranged by size:
472 363 230
PpuMI rG'GwC_Cy
Cuts at: 0 54 816 1065
Size: 54 762 249
Fragments arranged by size:
762 249 54
PspOMI G'GGCC_C
Cuts at: 0 304 1065
Size: 304 761
PstI C_TGCA'G
Cuts at: 0 475 1065
Size: 475 590
PvuII CAG'CTG
Cuts at: 0 641 1022 1065
Size: 641 381 43
Fragments arranged by size:
641 381 43
SapI GCTCTTCn'nnn_
Cuts at: 0 799 1065
Size: 799 266
ScaI AGT'ACT
Cuts at: 0 787 1065
Size: 787 278
SexAI A'CCwGG_T
Cuts at: 0 818 1065
Size: 818 247
SfcI C'TryA_G
Cuts at: 0 471 1065
Size: 471 594
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 367 1065
Size: 367 698
SmlI C'TyrA_G
Cuts at: 0 700 1065
Size: 700 365
StuI AGG'CCT
Cuts at: 0 149 1065
Size: 149 916
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 753 819 1065
Size: 753 66 246
Fragments arranged by size:
753 246 66
TatI w'GTAC_w
Cuts at: 0 101 785 1065
Size: 101 684 280
Fragments arranged by size:
684 280 101
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 736 768 1065
Size: 736 32 297
Fragments arranged by size:
736 297 32
Tth111I GACn'n_nGTC
Cuts at: 0 820 1065
Size: 820 245
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 147 919 1065
Size: 147 772 146
Fragments arranged by size:
772 147 146
Enzymes that do cut:
AarI AccI AlwNI ApaI ApaLI AvaI BanI BanII
BbsI BcgI BglI Bme1580I BmgBI BplI BpuEI BsaI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI
Bsp1286I BspMI BsrBI BsrDI BsrFI BssHII BssSI BstXI
BtgI BtgZI BtsI ClaI EaeI EagI EarI EcoNI
EcoO109I HaeII Hin4I MscI MspA1I NaeI NgoMIV NspI
PasI PfoI PpuMI PspOMI PstI PvuII SapI ScaI
SexAI SfcI SfiI SmlI StuI TaqII TatI TstI
Tth111I XcmI
Enzymes that do not cut:
AatII Acc65I AclI AcuI AfeI AflII AflIII AgeI
AhdI AleI AlfI AloI ApoI AscI AseI AsiSI
AvrII BaeI BamHI BbeI BbvCI BciVI BclI BfrBI
BglII BlpI BmrI BmtI BpmI Bpu10I BsaAI BsaBI
BsaHI BsiWI BsmI BspEI BspHI BsrGI BstAPI BstBI
BstEII BstYI BstZ17I Bsu36I CspCI DraI DraIII DrdI
EciI EcoICRI Eco57MI EcoRI EcoRV FalI FseI FspI
FspAI HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MslI NarI NcoI NdeI NheI NotI NruI
NsiI PacI PciI PflMI PmeI PmlI PpiI PshAI
PsiI PspXI PsrI PvuI RsrII SacI SacII SalI
SanDI SbfI SfoI SgfI SgrAI SmaI SnaBI SpeI
SphI SrfI SspI StyI SwaI XbaI XhoI XmaI
XmnI ZraI