Restriction map for
pF1KB7130
(Linear) (Six-base) MAPSORT of: KIBB7130.seq Check: 1599 from: 1 to: 948
KIBB7130.orf
With 182 enzymes: SgfI *
March 3, 2008 10:59 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 200 770 919 948
Size: 200 570 149 29
Fragments arranged by size:
570 200 149 29
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 140 172 948
Size: 140 32 776
Fragments arranged by size:
776 140 32
AlwNI CAG_nnn'CTG
Cuts at: 0 60 686 948
Size: 60 626 262
Fragments arranged by size:
626 262 60
BfrBI ATG'CAT
Cuts at: 0 468 948
Size: 468 480
BsaWI w'CCGG_w
Cuts at: 0 809 948
Size: 809 139
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 140 170 948
Size: 140 30 778
Fragments arranged by size:
778 140 30
BseYI C'CCAG_C
Cuts at: 0 290 948
Size: 290 658
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 273 467 948
Size: 273 194 481
Fragments arranged by size:
481 273 194
BsmI GAATG_Cn'
Cuts at: 0 468 948
Size: 468 480
BspHI T'CATG_A
Cuts at: 0 398 476 948
Size: 398 78 472
Fragments arranged by size:
472 398 78
BsrBI CCG'CTC
Cuts at: 0 543 948
Size: 543 405
BssSI C'ACGA_G
Cuts at: 0 247 948
Size: 247 701
BstXI CCAn_nnnn'nTGG
Cuts at: 0 336 948
Size: 336 612
BstYI r'GATC_y
Cuts at: 0 121 948
Size: 121 827
Bsu36I CC'TnA_GG
Cuts at: 0 871 948
Size: 871 77
BtgI C'CryG_G
Cuts at: 0 614 948
Size: 614 334
BtsI GCAGTG_nn'
Cuts at: 0 223 948
Size: 223 725
DraI TTT'AAA
Cuts at: 0 927 948
Size: 927 21
EaeI y'GGCC_r
Cuts at: 0 818 948
Size: 818 130
EarI CTCTTCn'nnn_
Cuts at: 0 386 641 948
Size: 386 255 307
Fragments arranged by size:
386 307 255
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 200 770 919 948
Size: 200 570 149 29
Fragments arranged by size:
570 200 149 29
EcoO109I rG'GnC_Cy
Cuts at: 0 683 948
Size: 683 265
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 561 924 948
Size: 561 363 24
Fragments arranged by size:
561 363 24
HaeII r_GCGC'y
Cuts at: 0 836 948
Size: 836 112
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 879 911 948
Size: 879 32 37
Fragments arranged by size:
879 37 32
MscI TGG'CCA
Cuts at: 0 820 948
Size: 820 128
MslI CAynn'nnrTG
Cuts at: 0 16 613 796 948
Size: 16 597 183 152
Fragments arranged by size:
597 183 152 16
MspA1I CmG'CkG
Cuts at: 0 430 948
Size: 430 518
NcoI C'CATG_G
Cuts at: 0 614 948
Size: 614 334
NsiI A_TGCA'T
Cuts at: 0 470 948
Size: 470 478
PflMI CCAn_nnn'nTGG
Cuts at: 0 312 732 948
Size: 312 420 216
Fragments arranged by size:
420 312 216
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 140 172 948
Size: 140 32 776
Fragments arranged by size:
776 140 32
PpuMI rG'GwC_Cy
Cuts at: 0 683 948
Size: 683 265
PstI C_TGCA'G
Cuts at: 0 65 691 948
Size: 65 626 257
Fragments arranged by size:
626 257 65
PvuII CAG'CTG
Cuts at: 0 430 948
Size: 430 518
SfcI C'TryA_G
Cuts at: 0 61 558 687 948
Size: 61 497 129 261
Fragments arranged by size:
497 261 129 61
StyI C'CwwG_G
Cuts at: 0 614 948
Size: 614 334
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 304 948
Size: 304 644
TatI w'GTAC_w
Cuts at: 0 846 948
Size: 846 102
XmnI GAAnn'nnTTC
Cuts at: 0 189 948
Size: 189 759
Enzymes that do cut:
AcuI AloI AlwNI BfrBI BsaWI BsaXI BseYI BsgI
BsmI BspHI BsrBI BssSI BstXI BstYI Bsu36I BtgI
BtsI DraI EaeI EarI Eco57MI EcoO109I FalI HaeII
Hin4I MscI MslI MspA1I NcoI NsiI PflMI PpiI
PpuMI PstI PvuII SfcI StyI TaqII TatI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AleI AlfI ApaI ApaLI ApoI AscI
AseI AsiSI AvaI AvrII BaeI BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BseRI BsiEI BsiHKAI
BsiWI BsmBI Bsp1286I BspEI BspMI BsrDI BsrFI BsrGI
BssHII BstAPI BstBI BstEII BstZ17I BtgZI ClaI CspCI
DraIII DrdI EagI EciI EcoICRI EcoNI EcoRI EcoRV
FseI FspI FspAI HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI NaeI NarI NdeI NgoMIV NheI
NotI NruI NspI PacI PasI PciI PfoI PmeI
PmlI PshAI PsiI PspOMI PspXI PsrI PvuI RsrII
SacI SacII SalI SanDI SapI SbfI ScaI SexAI
SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SrfI SspI StuI SwaI TstI Tth111I XbaI
XcmI XhoI XmaI ZraI