Restriction map for
pF1KB7127
(Linear) (Six-base) MAPSORT of: KIBB7127.seq Check: 4031 from: 1 to: 942
KIBB7127.orf
With 182 enzymes: SgfI *
March 3, 2008 10:58 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 547 942
Size: 547 395
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 56 435 942
Size: 56 379 507
Fragments arranged by size:
507 379 56
AhdI GACnn_n'nnGTC
Cuts at: 0 303 942
Size: 303 639
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 107 128 942
Size: 107 21 814
Fragments arranged by size:
814 107 21
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 295 327 942
Size: 295 32 615
Fragments arranged by size:
615 295 32
ApaI G_GGCC'C
Cuts at: 0 831 942
Size: 831 111
ApoI r'AATT_y
Cuts at: 0 808 942
Size: 808 134
AvaI C'yCGr_G
Cuts at: 0 53 942
Size: 53 889
BamHI G'GATC_C
Cuts at: 0 720 942
Size: 720 222
BanI G'GyrC_C
Cuts at: 0 156 942
Size: 156 786
BanII G_rGCy'C
Cuts at: 0 831 942
Size: 831 111
BbsI GAAGACnn'nnnn_
Cuts at: 0 710 942
Size: 710 232
Bme1580I G_kGCm'C
Cuts at: 0 648 831 942
Size: 648 183 111
Fragments arranged by size:
648 183 111
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 132 942
Size: 132 810
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 189 243 258 322 787 942
Size: 189 54 15 64 465 155
Fragments arranged by size:
465 189 155 64 54 15
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 28 67 349 942
Size: 28 39 282 593
Fragments arranged by size:
593 282 39 28
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 295 325 942
Size: 295 30 617
Fragments arranged by size:
617 295 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 30 71 108 111 115 289 352 667
Size: 30 41 37 3 4 174 63 315
Cuts at: 667 682 942
Size: 15 260
Fragments arranged by size:
315 260 174 63 41 37 30 15
4 3
BseYI C'CCAG_C
Cuts at: 0 405 567 942
Size: 405 162 375
Fragments arranged by size:
405 375 162
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 772 844 942
Size: 772 72 98
Fragments arranged by size:
772 98 72
Bsp1286I G_dGCh'C
Cuts at: 0 648 831 942
Size: 648 183 111
Fragments arranged by size:
648 183 111
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 547 768 942
Size: 547 221 174
Fragments arranged by size:
547 221 174
BsrFI r'CCGG_y
Cuts at: 0 384 773 942
Size: 384 389 169
Fragments arranged by size:
389 384 169
BsrGI T'GTAC_A
Cuts at: 0 524 942
Size: 524 418
BstXI CCAn_nnnn'nTGG
Cuts at: 0 543 942
Size: 543 399
BstYI r'GATC_y
Cuts at: 0 177 720 942
Size: 177 543 222
Fragments arranged by size:
543 222 177
Bsu36I CC'TnA_GG
Cuts at: 0 194 650 942
Size: 194 456 292
Fragments arranged by size:
456 292 194
BtsI GCAGTG_nn'
Cuts at: 0 24 795 942
Size: 24 771 147
Fragments arranged by size:
771 147 24
EaeI y'GGCC_r
Cuts at: 0 338 516 617 942
Size: 338 178 101 325
Fragments arranged by size:
338 325 178 101
EarI CTCTTCn'nnn_
Cuts at: 0 129 266 932 942
Size: 129 137 666 10
Fragments arranged by size:
666 137 129 10
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 56 189 243 258 322 435 787 942
Size: 56 133 54 15 64 113 352 155
Fragments arranged by size:
352 155 133 113 64 56 54 15
EcoNI CCTnn'n_nnAGG
Cuts at: 0 38 151 942
Size: 38 113 791
Fragments arranged by size:
791 113 38
EcoO109I rG'GnC_Cy
Cuts at: 0 63 780 827 828 886 942
Size: 63 717 47 1 58 56
Fragments arranged by size:
717 63 58 56 47 1
EcoRI G'AATT_C
Cuts at: 0 808 942
Size: 808 134
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 61 942
Size: 61 881
FspI TGC'GCA
Cuts at: 0 83 942
Size: 83 859
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 289 321 942
Size: 289 32 621
Fragments arranged by size:
621 289 32
HindIII A'AGCT_T
Cuts at: 0 458 942
Size: 458 484
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 399 942
Size: 399 543
MscI TGG'CCA
Cuts at: 0 619 942
Size: 619 323
MslI CAynn'nnrTG
Cuts at: 0 220 942
Size: 220 722
MspA1I CmG'CkG
Cuts at: 0 477 942
Size: 477 465
NdeI CA'TA_TG
Cuts at: 0 866 942
Size: 866 76
NspI r_CATG'y
Cuts at: 0 803 942
Size: 803 139
PasI CC'CwG_GG
Cuts at: 0 66 147 942
Size: 66 81 795
Fragments arranged by size:
795 81 66
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 295 327 942
Size: 295 32 615
Fragments arranged by size:
615 295 32
PpuMI rG'GwC_Cy
Cuts at: 0 780 886 942
Size: 780 106 56
Fragments arranged by size:
780 106 56
PspOMI G'GGCC_C
Cuts at: 0 827 942
Size: 827 115
PvuII CAG'CTG
Cuts at: 0 477 942
Size: 477 465
SmlI C'TyrA_G
Cuts at: 0 7 46 364 942
Size: 7 39 318 578
Fragments arranged by size:
578 318 39 7
StuI AGG'CCT
Cuts at: 0 44 599 942
Size: 44 555 343
Fragments arranged by size:
555 343 44
StyI C'CwwG_G
Cuts at: 0 590 823 942
Size: 590 233 119
Fragments arranged by size:
590 233 119
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 67 559 754 942
Size: 67 492 195 188
Fragments arranged by size:
492 195 188 67
TatI w'GTAC_w
Cuts at: 0 524 942
Size: 524 418
Enzymes that do cut:
AarI AcuI AhdI AlfI AloI ApaI ApoI AvaI
BamHI BanI BanII BbsI Bme1580I BplI BpmI BpuEI
BsaXI BseRI BseYI BsgI Bsp1286I BspMI BsrFI BsrGI
BstXI BstYI Bsu36I BtsI EaeI EarI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI Hin4I HindIII MmeI MscI
MslI MspA1I NdeI NspI PasI PpiI PpuMI PspOMI
PvuII SmlI StuI StyI TaqII TatI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AflIII AgeI
AleI AlwNI ApaLI AscI AseI AsiSI AvrII BaeI
BbeI BbvCI BcgI BciVI BclI BfrBI BglI BglII
BlpI BmgBI BmrI BmtI Bpu10I BsaI BsaAI BsaBI
BsaHI BsaWI BsiEI BsiHKAI BsiWI BsmI BsmBI BspEI
BspHI BsrBI BsrDI BssHII BssSI BstAPI BstBI BstEII
BstZ17I BtgI BtgZI ClaI CspCI DraI DraIII DrdI
EagI EciI EcoICRI EcoRV FseI FspAI HaeII HincII
HpaI KasI KpnI MfeI MluI NaeI NarI NcoI
NgoMIV NheI NotI NruI NsiI PacI PciI PflMI
PfoI PmeI PmlI PshAI PsiI PspXI PsrI PstI
PvuI RsrII SacI SacII SalI SanDI SapI SbfI
ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI
SnaBI SpeI SphI SrfI SspI SwaI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI