Restriction map for
pF1KB7125
(Linear) (Six-base) MAPSORT of: KIBB7125.seq Check: 9217 from: 1 to: 936
KIBB7125.orf
With 182 enzymes: SgfI *
March 3, 2008 10:58 ..
AccI GT'mk_AC
Cuts at: 0 23 936
Size: 23 913
AleI CACnn'nnGTG
Cuts at: 0 743 936
Size: 743 193
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 253 865 936
Size: 253 612 71
Fragments arranged by size:
612 253 71
ApoI r'AATT_y
Cuts at: 0 129 936
Size: 129 807
BamHI G'GATC_C
Cuts at: 0 53 936
Size: 53 883
BanII G_rGCy'C
Cuts at: 0 179 936
Size: 179 757
BbsI GAAGACnn'nnnn_
Cuts at: 0 741 936
Size: 741 195
BmtI G_CTAG'C
Cuts at: 0 230 936
Size: 230 706
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 739 936
Size: 739 197
BsaI GGTCTCn'nnnn_
Cuts at: 0 809 936
Size: 809 127
BsaBI GATnn'nnATC
Cuts at: 0 673 936
Size: 673 263
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 694 724 936
Size: 694 30 212
Fragments arranged by size:
694 212 30
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 94 936
Size: 94 842
BsmI GAATG_Cn'
Cuts at: 0 718 908 936
Size: 718 190 28
Fragments arranged by size:
718 190 28
Bsp1286I G_dGCh'C
Cuts at: 0 179 936
Size: 179 757
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 736 936
Size: 736 200
BsrDI GCAATG_nn'
Cuts at: 0 113 215 322 731 936
Size: 113 102 107 409 205
Fragments arranged by size:
409 205 113 107 102
BsrFI r'CCGG_y
Cuts at: 0 500 936
Size: 500 436
BsrGI T'GTAC_A
Cuts at: 0 182 936
Size: 182 754
BstXI CCAn_nnnn'nTGG
Cuts at: 0 363 936
Size: 363 573
BstYI r'GATC_y
Cuts at: 0 53 936
Size: 53 883
BtgI C'CryG_G
Cuts at: 0 356 936
Size: 356 580
BtsI GCAGTG_nn'
Cuts at: 0 82 936
Size: 82 854
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 198 233 936
Size: 198 35 703
Fragments arranged by size:
703 198 35
DraI TTT'AAA
Cuts at: 0 714 936
Size: 714 222
EaeI y'GGCC_r
Cuts at: 0 353 377 554 936
Size: 353 24 177 382
Fragments arranged by size:
382 353 177 24
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 739 936
Size: 739 197
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 623 655 936
Size: 623 32 281
Fragments arranged by size:
623 281 32
HincII GTy'rAC
Cuts at: 0 41 591 936
Size: 41 550 345
Fragments arranged by size:
550 345 41
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 35 936
Size: 35 901
MscI TGG'CCA
Cuts at: 0 355 379 556 936
Size: 355 24 177 380
Fragments arranged by size:
380 355 177 24
MslI CAynn'nnrTG
Cuts at: 0 118 349 743 913 936
Size: 118 231 394 170 23
Fragments arranged by size:
394 231 170 118 23
NcoI C'CATG_G
Cuts at: 0 356 936
Size: 356 580
NdeI CA'TA_TG
Cuts at: 0 551 936
Size: 551 385
NheI G'CTAG_C
Cuts at: 0 226 936
Size: 226 710
NspI r_CATG'y
Cuts at: 0 30 201 403 936
Size: 30 171 202 533
Fragments arranged by size:
533 202 171 30
ScaI AGT'ACT
Cuts at: 0 488 936
Size: 488 448
SfcI C'TryA_G
Cuts at: 0 623 936
Size: 623 313
SpeI A'CTAG_T
Cuts at: 0 45 936
Size: 45 891
SphI G_CATG'C
Cuts at: 0 201 936
Size: 201 735
SspI AAT'ATT
Cuts at: 0 257 936
Size: 257 679
StuI AGG'CCT
Cuts at: 0 62 936
Size: 62 874
StyI C'CwwG_G
Cuts at: 0 356 936
Size: 356 580
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 430 936
Size: 430 506
TatI w'GTAC_w
Cuts at: 0 182 486 936
Size: 182 304 450
Fragments arranged by size:
450 304 182
Enzymes that do cut:
AccI AleI AlfI ApoI BamHI BanII BbsI BmtI
BpmI BsaI BsaBI BsaXI BsgI BsmI Bsp1286I BspMI
BsrDI BsrFI BsrGI BstXI BstYI BtgI BtsI CspCI
DraI EaeI Eco57MI Hin4I HincII MmeI MscI MslI
NcoI NdeI NheI NspI ScaI SfcI SpeI SphI
SspI StuI StyI TaqII TatI
Enzymes that do not cut:
AarI AatII Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AloI AlwNI ApaI ApaLI AscI AseI
AsiSI AvaI AvrII BaeI BanI BbeI BbvCI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BplI Bpu10I BpuEI BsaAI BsaHI BsaWI BseRI
BseYI BsiEI BsiHKAI BsiWI BsmBI BspEI BspHI BsrBI
BssHII BssSI BstAPI BstBI BstEII BstZ17I Bsu36I BtgZI
ClaI DraIII DrdI EagI EarI EciI EcoICRI EcoNI
EcoO109I EcoRI EcoRV FalI FseI FspI FspAI HaeII
HindIII HpaI KasI KpnI MfeI MluI MspA1I NaeI
NarI NgoMIV NotI NruI NsiI PacI PasI PciI
PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI
PspOMI PspXI PsrI PstI PvuI PvuII RsrII SacI
SacII SalI SanDI SapI SbfI SexAI SfiI SfoI
SgfI SgrAI SmaI SmlI SnaBI SrfI SwaI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI