Restriction map for
pF1KB7123
(Linear) (Six-base) MAPSORT of: KIBB7123.seq Check: 7109 from: 1 to: 933
KIBB7123.orf
With 182 enzymes: SgfI *
March 3, 2008 10:58 ..
AccI GT'mk_AC
Cuts at: 0 814 933
Size: 814 119
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 209 594 933
Size: 209 385 339
Fragments arranged by size:
385 339 209
AleI CACnn'nnGTG
Cuts at: 0 499 933
Size: 499 434
ApaLI G'TGCA_C
Cuts at: 0 502 933
Size: 502 431
ApoI r'AATT_y
Cuts at: 0 594 933
Size: 594 339
BamHI G'GATC_C
Cuts at: 0 114 933
Size: 114 819
BanI G'GyrC_C
Cuts at: 0 406 556 933
Size: 406 150 377
Fragments arranged by size:
406 377 150
BanII G_rGCy'C
Cuts at: 0 400 578 933
Size: 400 178 355
Fragments arranged by size:
400 355 178
BbeI G_GCGC'C
Cuts at: 0 410 933
Size: 410 523
BclI T'GATC_A
Cuts at: 0 344 933
Size: 344 589
BglI GCCn_nnn'nGGC
Cuts at: 0 404 514 933
Size: 404 110 419
Fragments arranged by size:
419 404 110
Bme1580I G_kGCm'C
Cuts at: 0 506 546 933
Size: 506 40 387
Fragments arranged by size:
506 387 40
BmrI ACTGGGnnnn_n'
Cuts at: 0 556 933
Size: 556 377
BsaI GGTCTCn'nnnn_
Cuts at: 0 329 933
Size: 329 604
BsaHI Gr'CG_yC
Cuts at: 0 407 933
Size: 407 526
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 77 107 326 356 637 667 796 826
Size: 77 30 219 30 281 30 129 30
Cuts at: 826 933
Size: 107
Fragments arranged by size:
281 219 129 107 77 30 30 30
30
BseYI C'CCAG_C
Cuts at: 0 298 399 519 820 933
Size: 298 101 120 301 113
Fragments arranged by size:
301 298 120 113 101
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 55 92 296 933
Size: 55 37 204 637
Fragments arranged by size:
637 204 55 37
BsiHKAI G_wGCw'C
Cuts at: 0 506 933
Size: 506 427
Bsp1286I G_dGCh'C
Cuts at: 0 400 506 546 578 933
Size: 400 106 40 32 355
Fragments arranged by size:
400 355 106 40 32
BsrBI CCG'CTC
Cuts at: 0 168 929 933
Size: 168 761 4
Fragments arranged by size:
761 168 4
BsrDI GCAATG_nn'
Cuts at: 0 231 933
Size: 231 702
BstAPI GCAn_nnn'nTGC
Cuts at: 0 510 724 933
Size: 510 214 209
Fragments arranged by size:
510 214 209
BstXI CCAn_nnnn'nTGG
Cuts at: 0 330 471 933
Size: 330 141 462
Fragments arranged by size:
462 330 141
BstYI r'GATC_y
Cuts at: 0 114 933
Size: 114 819
BstZ17I GTA'TAC
Cuts at: 0 815 933
Size: 815 118
Bsu36I CC'TnA_GG
Cuts at: 0 149 933
Size: 149 784
BtgI C'CryG_G
Cuts at: 0 119 362 677 933
Size: 119 243 315 256
Fragments arranged by size:
315 256 243 119
BtsI GCAGTG_nn'
Cuts at: 0 494 632 933
Size: 494 138 301
Fragments arranged by size:
494 301 138
EaeI y'GGCC_r
Cuts at: 0 461 933
Size: 461 472
EarI CTCTTCn'nnn_
Cuts at: 0 112 196 933
Size: 112 84 737
Fragments arranged by size:
737 112 84
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 33 408 933
Size: 33 375 525
Fragments arranged by size:
525 375 33
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 209 594 933
Size: 209 385 339
Fragments arranged by size:
385 339 209
EcoNI CCTnn'n_nnAGG
Cuts at: 0 462 933
Size: 462 471
EcoO109I rG'GnC_Cy
Cuts at: 0 7 536 586 933
Size: 7 529 50 347
Fragments arranged by size:
529 347 50 7
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 474 933
Size: 474 459
FspI TGC'GCA
Cuts at: 0 781 933
Size: 781 152
HaeII r_GCGC'y
Cuts at: 0 410 933
Size: 410 523
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 77 109 903 933
Size: 77 32 794 30
Fragments arranged by size:
794 77 32 30
HindIII A'AGCT_T
Cuts at: 0 754 933
Size: 754 179
KasI G'GCGC_C
Cuts at: 0 406 933
Size: 406 527
MscI TGG'CCA
Cuts at: 0 463 933
Size: 463 470
MslI CAynn'nnrTG
Cuts at: 0 499 933
Size: 499 434
MspA1I CmG'CkG
Cuts at: 0 180 824 933
Size: 180 644 109
Fragments arranged by size:
644 180 109
NarI GG'CG_CC
Cuts at: 0 407 933
Size: 407 526
PasI CC'CwG_GG
Cuts at: 0 570 933
Size: 570 363
PflMI CCAn_nnn'nTGG
Cuts at: 0 363 933
Size: 363 570
PfoI T'CCnGG_A
Cuts at: 0 110 933
Size: 110 823
PpuMI rG'GwC_Cy
Cuts at: 0 7 536 933
Size: 7 529 397
Fragments arranged by size:
529 397 7
PstI C_TGCA'G
Cuts at: 0 185 720 899 933
Size: 185 535 179 34
Fragments arranged by size:
535 185 179 34
PvuII CAG'CTG
Cuts at: 0 180 933
Size: 180 753
RsrII CG'GwC_CG
Cuts at: 0 20 933
Size: 20 913
ScaI AGT'ACT
Cuts at: 0 142 933
Size: 142 791
SfcI C'TryA_G
Cuts at: 0 181 205 716 895 933
Size: 181 24 511 179 38
Fragments arranged by size:
511 181 179 38 24
SfoI GGC'GCC
Cuts at: 0 408 933
Size: 408 525
StuI AGG'CCT
Cuts at: 0 53 77 933
Size: 53 24 856
Fragments arranged by size:
856 53 24
StyI C'CwwG_G
Cuts at: 0 458 933
Size: 458 475
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 544 834 933
Size: 544 290 99
Fragments arranged by size:
544 290 99
TatI w'GTAC_w
Cuts at: 0 140 563 933
Size: 140 423 370
Fragments arranged by size:
423 370 140
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 326 342 358 374 933
Size: 326 16 16 16 559
Fragments arranged by size:
559 326 16 16 16
Tth111I GACn'n_nGTC
Cuts at: 0 432 933
Size: 432 501
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 567 933
Size: 567 366
Enzymes that do cut:
AccI AcuI AleI ApaLI ApoI BamHI BanI BanII
BbeI BclI BglI Bme1580I BmrI BsaI BsaHI BsaXI
BseYI BsgI BsiHKAI Bsp1286I BsrBI BsrDI BstAPI BstXI
BstYI BstZ17I Bsu36I BtgI BtsI EaeI EarI EciI
Eco57MI EcoNI EcoO109I FalI FspI HaeII Hin4I HindIII
KasI MscI MslI MspA1I NarI PasI PflMI PfoI
PpuMI PstI PvuII RsrII ScaI SfcI SfoI StuI
StyI TaqII TatI TstI Tth111I XcmI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AflIII AgeI
AhdI AlfI AloI AlwNI ApaI AscI AseI AsiSI
AvaI AvrII BaeI BbsI BbvCI BcgI BciVI BfrBI
BglII BlpI BmgBI BmtI BplI BpmI Bpu10I BpuEI
BsaAI BsaBI BsaWI BseRI BsiEI BsiWI BsmI BsmBI
BspEI BspHI BspMI BsrFI BsrGI BssHII BssSI BstBI
BstEII BtgZI ClaI CspCI DraI DraIII DrdI EagI
EcoICRI EcoRI EcoRV FseI FspAI HincII HpaI KpnI
MfeI MluI MmeI NaeI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspI PacI PciI PmeI PmlI
PpiI PshAI PsiI PspOMI PspXI PsrI PvuI SacI
SacII SalI SanDI SapI SbfI SexAI SfiI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI
SwaI XbaI XhoI XmaI XmnI ZraI