Restriction map for
pF1KB7122
(Linear) (Six-base) MAPSORT of: KIBB7122.seq Check: 803 from: 1 to: 927
KIBB7122.orf
With 182 enzymes: SgfI *
March 3, 2008 10:58 ..
AccI GT'mk_AC
Cuts at: 0 242 927
Size: 242 685
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 688 927
Size: 688 239
AleI CACnn'nnGTG
Cuts at: 0 411 927
Size: 411 516
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 193 225 927
Size: 193 32 702
Fragments arranged by size:
702 193 32
AlwNI CAG_nnn'CTG
Cuts at: 0 156 927
Size: 156 771
ApoI r'AATT_y
Cuts at: 0 25 927
Size: 25 902
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 76 927
Size: 76 851
BsaI GGTCTCn'nnnn_
Cuts at: 0 46 927
Size: 46 881
BsaAI yAC'GTr
Cuts at: 0 363 927
Size: 363 564
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 235 265 927
Size: 235 30 662
Fragments arranged by size:
662 235 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 325 927
Size: 325 602
BseYI C'CCAG_C
Cuts at: 0 290 919 927
Size: 290 629 8
Fragments arranged by size:
629 290 8
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 367 543 927
Size: 367 176 384
Fragments arranged by size:
384 367 176
BsiHKAI G_wGCw'C
Cuts at: 0 262 927
Size: 262 665
BsmI GAATG_Cn'
Cuts at: 0 870 927
Size: 870 57
Bsp1286I G_dGCh'C
Cuts at: 0 262 927
Size: 262 665
BspHI T'CATG_A
Cuts at: 0 398 927
Size: 398 529
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 718 927
Size: 718 209
BsrDI GCAATG_nn'
Cuts at: 0 467 809 927
Size: 467 342 118
Fragments arranged by size:
467 342 118
BsrGI T'GTAC_A
Cuts at: 0 764 927
Size: 764 163
BstEII G'GTnAC_C
Cuts at: 0 161 927
Size: 161 766
BstXI CCAn_nnnn'nTGG
Cuts at: 0 111 927
Size: 111 816
BtgI C'CryG_G
Cuts at: 0 406 927
Size: 406 521
EaeI y'GGCC_r
Cuts at: 0 365 617 927
Size: 365 252 310
Fragments arranged by size:
365 310 252
EarI CTCTTCn'nnn_
Cuts at: 0 481 927
Size: 481 446
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 76 688 927
Size: 76 612 239
Fragments arranged by size:
612 239 76
EcoRI G'AATT_C
Cuts at: 0 25 927
Size: 25 902
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 235 267 927
Size: 235 32 660
Fragments arranged by size:
660 235 32
HincII GTy'rAC
Cuts at: 0 548 927
Size: 548 379
MscI TGG'CCA
Cuts at: 0 367 619 927
Size: 367 252 308
Fragments arranged by size:
367 308 252
MslI CAynn'nnrTG
Cuts at: 0 343 411 832 927
Size: 343 68 421 95
Fragments arranged by size:
421 343 95 68
MspA1I CmG'CkG
Cuts at: 0 294 927
Size: 294 633
PflMI CCAn_nnn'nTGG
Cuts at: 0 576 927
Size: 576 351
PstI C_TGCA'G
Cuts at: 0 715 927
Size: 715 212
PvuII CAG'CTG
Cuts at: 0 294 927
Size: 294 633
SfcI C'TryA_G
Cuts at: 0 711 927
Size: 711 216
SspI AAT'ATT
Cuts at: 0 71 927
Size: 71 856
StuI AGG'CCT
Cuts at: 0 774 927
Size: 774 153
StyI C'CwwG_G
Cuts at: 0 230 927
Size: 230 697
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 304 429 583 846 927
Size: 304 125 154 263 81
Fragments arranged by size:
304 263 154 125 81
TatI w'GTAC_w
Cuts at: 0 764 927
Size: 764 163
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 597 629 927
Size: 597 32 298
Fragments arranged by size:
597 298 32
XmnI GAAnn'nnTTC
Cuts at: 0 29 927
Size: 29 898
Enzymes that do cut:
AccI AcuI AleI AloI AlwNI ApoI BpmI BsaI
BsaAI BsaXI BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I
BspHI BspMI BsrDI BsrGI BstEII BstXI BtgI EaeI
EarI Eco57MI EcoRI Hin4I HincII MscI MslI MspA1I
PflMI PstI PvuII SfcI SspI StuI StyI TaqII
TatI TstI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AflIII AgeI
AhdI AlfI ApaI ApaLI AscI AseI AsiSI AvaI
AvrII BaeI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI Bpu10I BpuEI BsaBI BsaHI
BsaWI BsiEI BsiWI BsmBI BspEI BsrBI BsrFI BssHII
BssSI BstAPI BstBI BstYI BstZ17I Bsu36I BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EagI EciI EcoICRI
EcoNI EcoO109I EcoRV FalI FseI FspI FspAI HaeII
HindIII HpaI KasI KpnI MfeI MluI MmeI NaeI
NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI
NspI PacI PasI PciI PfoI PmeI PmlI PpiI
PpuMI PshAI PsiI PspOMI PspXI PsrI PvuI RsrII
SacI SacII SalI SanDI SapI SbfI ScaI SexAI
SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SrfI SwaI Tth111I XbaI XcmI XhoI XmaI
ZraI