Restriction map for
pF1KB7121
(Linear) (Six-base) MAPSORT of: KIBB7121.seq Check: 1986 from: 1 to: 915
KIBB7121.orf
With 182 enzymes: SgfI *
March 3, 2008 10:58 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 211 491 822 915
Size: 211 280 331 93
Fragments arranged by size:
331 280 211 93
AlwNI CAG_nnn'CTG
Cuts at: 0 59 915
Size: 59 856
AvaI C'yCGr_G
Cuts at: 0 632 915
Size: 632 283
BamHI G'GATC_C
Cuts at: 0 563 915
Size: 563 352
BanII G_rGCy'C
Cuts at: 0 8 379 678 707 776 915
Size: 8 371 299 29 69 139
Fragments arranged by size:
371 299 139 69 29 8
BbsI GAAGACnn'nnnn_
Cuts at: 0 497 915
Size: 497 418
BclI T'GATC_A
Cuts at: 0 575 915
Size: 575 340
BmgBI CAC'GTC
Cuts at: 0 503 915
Size: 503 412
BmrI ACTGGGnnnn_n'
Cuts at: 0 569 915
Size: 569 346
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 133 915
Size: 133 782
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 81 173 670 915
Size: 81 92 497 245
Fragments arranged by size:
497 245 92 81
BsaHI Gr'CG_yC
Cuts at: 0 728 915
Size: 728 187
BsaWI w'CCGG_w
Cuts at: 0 371 915
Size: 371 544
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 192 195 490 703 915
Size: 192 3 295 213 212
Fragments arranged by size:
295 213 212 192 3
BseYI C'CCAG_C
Cuts at: 0 419 669 720 915
Size: 419 250 51 195
Fragments arranged by size:
419 250 195 51
BsiHKAI G_wGCw'C
Cuts at: 0 8 379 776 915
Size: 8 371 397 139
Fragments arranged by size:
397 371 139 8
Bsp1286I G_dGCh'C
Cuts at: 0 8 379 678 707 776 915
Size: 8 371 299 29 69 139
Fragments arranged by size:
371 299 139 69 29 8
BsrGI T'GTAC_A
Cuts at: 0 906 915
Size: 906 9
BssSI C'ACGA_G
Cuts at: 0 571 915
Size: 571 344
BstEII G'GTnAC_C
Cuts at: 0 82 915
Size: 82 833
BstXI CCAn_nnnn'nTGG
Cuts at: 0 437 624 915
Size: 437 187 291
Fragments arranged by size:
437 291 187
BstYI r'GATC_y
Cuts at: 0 563 915
Size: 563 352
BtgI C'CryG_G
Cuts at: 0 595 915
Size: 595 320
EarI CTCTTCn'nnn_
Cuts at: 0 19 230 467 804 915
Size: 19 211 237 337 111
Fragments arranged by size:
337 237 211 111 19
EcoICRI GAG'CTC
Cuts at: 0 6 377 774 915
Size: 6 371 397 141
Fragments arranged by size:
397 371 141 6
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 81 173 211 491 670 822 915
Size: 81 92 38 280 179 152 93
Fragments arranged by size:
280 179 152 93 92 81 38
EcoNI CCTnn'n_nnAGG
Cuts at: 0 652 915
Size: 652 263
EcoRV GAT'ATC
Cuts at: 0 467 915
Size: 467 448
HaeII r_GCGC'y
Cuts at: 0 542 648 915
Size: 542 106 267
Fragments arranged by size:
542 267 106
MspA1I CmG'CkG
Cuts at: 0 283 366 689 915
Size: 283 83 323 226
Fragments arranged by size:
323 283 226 83
PasI CC'CwG_GG
Cuts at: 0 406 915
Size: 406 509
PflMI CCAn_nnn'nTGG
Cuts at: 0 59 915
Size: 59 856
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 361 393 915
Size: 361 32 522
Fragments arranged by size:
522 361 32
PstI C_TGCA'G
Cuts at: 0 310 915
Size: 310 605
SacI G_AGCT'C
Cuts at: 0 8 379 776 915
Size: 8 371 397 139
Fragments arranged by size:
397 371 139 8
SapI GCTCTTCn'nnn_
Cuts at: 0 19 915
Size: 19 896
ScaI AGT'ACT
Cuts at: 0 222 915
Size: 222 693
SfcI C'TryA_G
Cuts at: 0 306 518 915
Size: 306 212 397
Fragments arranged by size:
397 306 212
SmaI CCC'GGG
Cuts at: 0 634 915
Size: 634 281
StyI C'CwwG_G
Cuts at: 0 881 915
Size: 881 34
TatI w'GTAC_w
Cuts at: 0 220 906 915
Size: 220 686 9
Fragments arranged by size:
686 220 9
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 549 915
Size: 549 366
XmaI C'CCGG_G
Cuts at: 0 632 915
Size: 632 283
Enzymes that do cut:
AcuI AlwNI AvaI BamHI BanII BbsI BclI BmgBI
BmrI BplI BpmI BsaHI BsaWI BseRI BseYI BsiHKAI
Bsp1286I BsrGI BssSI BstEII BstXI BstYI BtgI EarI
EcoICRI Eco57MI EcoNI EcoRV HaeII MspA1I PasI PflMI
PpiI PstI SacI SapI ScaI SfcI SmaI StyI
TatI XcmI XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI ApaI ApaLI ApoI
AscI AseI AsiSI AvrII BaeI BanI BbeI BbvCI
BcgI BciVI BfrBI BglI BglII BlpI Bme1580I BmtI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaXI BsgI BsiEI
BsiWI BsmI BsmBI BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssHII BstAPI BstBI BstZ17I Bsu36I BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EciI
EcoO109I EcoRI FalI FseI FspI FspAI Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI NaeI NarI NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspI PacI PciI PfoI PmeI PmlI
PpuMI PshAI PsiI PspOMI PspXI PsrI PvuI PvuII
RsrII SacII SalI SanDI SbfI SexAI SfiI SfoI
SgfI SgrAI SmlI SnaBI SpeI SphI SrfI SspI
StuI SwaI TaqII TstI Tth111I XbaI XhoI XmnI
ZraI