Restriction map for
pF1KB7115
(Linear) (Six-base) MAPSORT of: KIBB7115.seq Check: 5729 from: 1 to: 837
KIBB7115.orf
With 182 enzymes: SgfI *
March 3, 2008 10:58 ..
AfeI AGC'GCT
Cuts at: 0 568 837
Size: 568 269
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 217 837
Size: 217 620
AlwNI CAG_nnn'CTG
Cuts at: 0 84 617 837
Size: 84 533 220
Fragments arranged by size:
533 220 84
AvaI C'yCGr_G
Cuts at: 0 72 807 837
Size: 72 735 30
Fragments arranged by size:
735 72 30
BamHI G'GATC_C
Cuts at: 0 446 837
Size: 446 391
BanI G'GyrC_C
Cuts at: 0 438 512 837
Size: 438 74 325
Fragments arranged by size:
438 325 74
BbeI G_GCGC'C
Cuts at: 0 516 837
Size: 516 321
BclI T'GATC_A
Cuts at: 0 302 837
Size: 302 535
BglI GCCn_nnn'nGGC
Cuts at: 0 700 837
Size: 700 137
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 265 280 837
Size: 265 15 557
Fragments arranged by size:
557 265 15
BsaHI Gr'CG_yC
Cuts at: 0 513 837
Size: 513 324
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 727 757 837
Size: 727 30 80
Fragments arranged by size:
727 80 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 262 394 667 676 837
Size: 262 132 273 9 161
Fragments arranged by size:
273 262 161 132 9
BseYI C'CCAG_C
Cuts at: 0 108 421 638 784 837
Size: 108 313 217 146 53
Fragments arranged by size:
313 217 146 108 53
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 465 837
Size: 465 372
BsiEI CG_ry'CG
Cuts at: 0 338 837
Size: 338 499
BsiHKAI G_wGCw'C
Cuts at: 0 206 434 559 837
Size: 206 228 125 278
Fragments arranged by size:
278 228 206 125
BsmBI CGTCTCn'nnnn_
Cuts at: 0 268 837
Size: 268 569
Bsp1286I G_dGCh'C
Cuts at: 0 206 434 559 837
Size: 206 228 125 278
Fragments arranged by size:
278 228 206 125
BssSI C'ACGA_G
Cuts at: 0 507 837
Size: 507 330
BstAPI GCAn_nnn'nTGC
Cuts at: 0 479 617 837
Size: 479 138 220
Fragments arranged by size:
479 220 138
BstXI CCAn_nnnn'nTGG
Cuts at: 0 792 837
Size: 792 45
BstYI r'GATC_y
Cuts at: 0 446 837
Size: 446 391
BtgI C'CryG_G
Cuts at: 0 407 461 837
Size: 407 54 376
Fragments arranged by size:
407 376 54
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 27 837
Size: 27 810
BtsI GCAGTG_nn'
Cuts at: 0 56 519 837
Size: 56 463 318
Fragments arranged by size:
463 318 56
EaeI y'GGCC_r
Cuts at: 0 14 311 335 632 837
Size: 14 297 24 297 205
Fragments arranged by size:
297 297 205 24 14
EagI C'GGCC_G
Cuts at: 0 335 837
Size: 335 502
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 673 837
Size: 673 164
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 265 280 837
Size: 265 15 557
Fragments arranged by size:
557 265 15
EcoNI CCTnn'n_nnAGG
Cuts at: 0 247 837
Size: 247 590
EcoO109I rG'GnC_Cy
Cuts at: 0 68 677 837
Size: 68 609 160
Fragments arranged by size:
609 160 68
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 339 552 837
Size: 339 213 285
Fragments arranged by size:
339 285 213
HaeII r_GCGC'y
Cuts at: 0 91 357 516 570 837
Size: 91 266 159 54 267
Fragments arranged by size:
267 266 159 91 54
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 727 759 837
Size: 727 32 78
Fragments arranged by size:
727 78 32
KasI G'GCGC_C
Cuts at: 0 512 837
Size: 512 325
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 770 837
Size: 770 67
MscI TGG'CCA
Cuts at: 0 16 837
Size: 16 821
MslI CAynn'nnrTG
Cuts at: 0 184 472 837
Size: 184 288 365
Fragments arranged by size:
365 288 184
MspA1I CmG'CkG
Cuts at: 0 84 296 645 837
Size: 84 212 349 192
Fragments arranged by size:
349 212 192 84
NarI GG'CG_CC
Cuts at: 0 513 837
Size: 513 324
NspI r_CATG'y
Cuts at: 0 477 837
Size: 477 360
PasI CC'CwG_GG
Cuts at: 0 682 837
Size: 682 155
PpuMI rG'GwC_Cy
Cuts at: 0 677 837
Size: 677 160
PstI C_TGCA'G
Cuts at: 0 284 613 837
Size: 284 329 224
Fragments arranged by size:
329 284 224
SanDI GG'GwC_CC
Cuts at: 0 677 837
Size: 677 160
SfcI C'TryA_G
Cuts at: 0 280 609 837
Size: 280 329 228
Fragments arranged by size:
329 280 228
SfoI GGC'GCC
Cuts at: 0 514 837
Size: 514 323
SphI G_CATG'C
Cuts at: 0 477 837
Size: 477 360
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 431 463 837
Size: 431 32 374
Fragments arranged by size:
431 374 32
Enzymes that do cut:
AfeI AlfI AlwNI AvaI BamHI BanI BbeI BclI
BglI BpmI BsaHI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmBI Bsp1286I BssSI BstAPI BstXI BstYI BtgI
BtgZI BtsI EaeI EagI EciI Eco57MI EcoNI EcoO109I
FalI HaeII Hin4I KasI MmeI MscI MslI MspA1I
NarI NspI PasI PpuMI PstI SanDI SfcI SfoI
SphI TstI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AloI ApaI ApaLI ApoI AscI
AseI AsiSI AvrII BaeI BanII BbsI BbvCI BcgI
BciVI BfrBI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI Bpu10I BpuEI BsaI BsaAI BsaBI BsaWI BsiWI
BsmI BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BstBI BstEII BstZ17I Bsu36I ClaI CspCI DraI
DraIII DrdI EarI EcoICRI EcoRI EcoRV FseI FspI
FspAI HincII HindIII HpaI KpnI MfeI MluI NaeI
NcoI NdeI NgoMIV NheI NotI NruI NsiI PacI
PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI
PspOMI PspXI PsrI PvuI PvuII RsrII SacI SacII
SalI SapI SbfI ScaI SexAI SfiI SgfI SgrAI
SmaI SmlI SnaBI SpeI SrfI SspI StuI StyI
SwaI TaqII TatI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI