Restriction map for
pF1KB7103
(Linear) (Six-base) MAPSORT of: KIBB7103.seq Check: 6867 from: 1 to: 657
KIBB7103.orf
With 182 enzymes: SgfI *
March 3, 2008 10:58 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 54 657
Size: 54 603
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 573 657
Size: 573 84
BanII G_rGCy'C
Cuts at: 0 265 475 657
Size: 265 210 182
Fragments arranged by size:
265 210 182
BglI GCCn_nnn'nGGC
Cuts at: 0 400 572 657
Size: 400 172 85
Fragments arranged by size:
400 172 85
Bme1580I G_kGCm'C
Cuts at: 0 214 657
Size: 214 443
BmtI G_CTAG'C
Cuts at: 0 205 657
Size: 205 452
Bpu10I CC'TnA_GC
Cuts at: 0 186 657
Size: 186 471
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2 657
Size: 2 655
BsaI GGTCTCn'nnnn_
Cuts at: 0 622 657
Size: 622 35
BsaHI Gr'CG_yC
Cuts at: 0 338 621 657
Size: 338 283 36
Fragments arranged by size:
338 283 36
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 61 91 514 544 657
Size: 61 30 423 30 113
Fragments arranged by size:
423 113 61 30 30
BseYI C'CCAG_C
Cuts at: 0 83 486 657
Size: 83 403 171
Fragments arranged by size:
403 171 83
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 292 657
Size: 292 365
BsiEI CG_ry'CG
Cuts at: 0 427 657
Size: 427 230
BsiHKAI G_wGCw'C
Cuts at: 0 221 265 657
Size: 221 44 392
Fragments arranged by size:
392 221 44
Bsp1286I G_dGCh'C
Cuts at: 0 214 221 265 475 657
Size: 214 7 44 210 182
Fragments arranged by size:
214 210 182 44 7
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 54 657
Size: 54 603
BsrBI CCG'CTC
Cuts at: 0 185 518 657
Size: 185 333 139
Fragments arranged by size:
333 185 139
BsrDI GCAATG_nn'
Cuts at: 0 452 641 657
Size: 452 189 16
Fragments arranged by size:
452 189 16
BsrFI r'CCGG_y
Cuts at: 0 379 617 657
Size: 379 238 40
Fragments arranged by size:
379 238 40
BssSI C'ACGA_G
Cuts at: 0 130 343 364 625 657
Size: 130 213 21 261 32
Fragments arranged by size:
261 213 130 32 21
BstEII G'GTnAC_C
Cuts at: 0 375 657
Size: 375 282
BstXI CCAn_nnnn'nTGG
Cuts at: 0 540 657
Size: 540 117
BtgI C'CryG_G
Cuts at: 0 91 122 567 576 657
Size: 91 31 445 9 81
Fragments arranged by size:
445 91 81 31 9
BtsI GCAGTG_nn'
Cuts at: 0 160 327 522 657
Size: 160 167 195 135
Fragments arranged by size:
195 167 160 135
DraIII CAC_nnn'GTG
Cuts at: 0 306 657
Size: 306 351
EaeI y'GGCC_r
Cuts at: 0 119 392 401 424 431 542 564 573
Size: 119 273 9 23 7 111 22 9
Cuts at: 573 615 657
Size: 42 42
Fragments arranged by size:
273 119 111 42 42 23 22 9
9 7
EagI C'GGCC_G
Cuts at: 0 424 657
Size: 424 233
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 280 657
Size: 280 377
EcoICRI GAG'CTC
Cuts at: 0 263 657
Size: 263 394
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 573 657
Size: 573 84
FspI TGC'GCA
Cuts at: 0 319 657
Size: 319 338
HaeII r_GCGC'y
Cuts at: 0 99 106 657
Size: 99 7 551
Fragments arranged by size:
551 99 7
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 619 651 657
Size: 619 32 6
Fragments arranged by size:
619 32 6
HincII GTy'rAC
Cuts at: 0 170 657
Size: 170 487
MscI TGG'CCA
Cuts at: 0 544 566 657
Size: 544 22 91
Fragments arranged by size:
544 91 22
MslI CAynn'nnrTG
Cuts at: 0 538 657
Size: 538 119
MspA1I CmG'CkG
Cuts at: 0 93 255 429 657
Size: 93 162 174 228
Fragments arranged by size:
228 174 162 93
NaeI GCC'GGC
Cuts at: 0 619 657
Size: 619 38
NcoI C'CATG_G
Cuts at: 0 567 576 657
Size: 567 9 81
Fragments arranged by size:
567 81 9
NgoMIV G'CCGG_C
Cuts at: 0 617 657
Size: 617 40
NheI G'CTAG_C
Cuts at: 0 201 657
Size: 201 456
NspI r_CATG'y
Cuts at: 0 54 657
Size: 54 603
PasI CC'CwG_GG
Cuts at: 0 25 657
Size: 25 632
PflMI CCAn_nnn'nTGG
Cuts at: 0 306 657
Size: 306 351
PshAI GACnn'nnGTC
Cuts at: 0 606 657
Size: 606 51
PvuII CAG'CTG
Cuts at: 0 255 657
Size: 255 402
SacI G_AGCT'C
Cuts at: 0 265 657
Size: 265 392
SacII CC_GC'GG
Cuts at: 0 94 657
Size: 94 563
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 400 572 657
Size: 400 172 85
Fragments arranged by size:
400 172 85
SmlI C'TyrA_G
Cuts at: 0 17 657
Size: 17 640
StyI C'CwwG_G
Cuts at: 0 467 567 576 657
Size: 467 100 9 81
Fragments arranged by size:
467 100 81 9
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 499 571 657
Size: 499 72 86
Fragments arranged by size:
499 86 72
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 61 93 514 546 657
Size: 61 32 421 32 111
Fragments arranged by size:
421 111 61 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 444 565 574 657
Size: 444 121 9 83
Fragments arranged by size:
444 121 83 9
Enzymes that do cut:
AarI AcuI BanII BglI Bme1580I BmtI Bpu10I BpuEI
BsaI BsaHI BsaXI BseYI BsgI BsiEI BsiHKAI Bsp1286I
BspMI BsrBI BsrDI BsrFI BssSI BstEII BstXI BtgI
BtsI DraIII EaeI EagI EciI EcoICRI Eco57MI FspI
HaeII Hin4I HincII MscI MslI MspA1I NaeI NcoI
NgoMIV NheI NspI PasI PflMI PshAI PvuII SacI
SacII SfiI SmlI StyI TaqII TstI XcmI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AscI AseI AsiSI AvaI AvrII BaeI BamHI BanI
BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglII
BlpI BmgBI BmrI BplI BpmI BsaAI BsaBI BsaWI
BseRI BsiWI BsmI BsmBI BspEI BspHI BsrGI BssHII
BstAPI BstBI BstYI BstZ17I Bsu36I BtgZI ClaI CspCI
DraI DrdI EarI EcoNI EcoO109I EcoRI EcoRV FalI
FseI FspAI HindIII HpaI KasI KpnI MfeI MluI
MmeI NarI NdeI NotI NruI NsiI PacI PciI
PfoI PmeI PmlI PpiI PpuMI PsiI PspOMI PspXI
PsrI PstI PvuI RsrII SalI SanDI SapI SbfI
ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SnaBI
SpeI SphI SrfI SspI StuI SwaI TatI Tth111I
XbaI XhoI XmaI XmnI ZraI