Restriction map for
pF1KB7102
(Linear) (Six-base) MAPSORT of: KIBB7102.seq Check: 4976 from: 1 to: 651
KIBB7102.orf
With 182 enzymes: SgfI *
March 3, 2008 10:58 ..
AatII G_ACGT'C
Cuts at: 0 491 651
Size: 491 160
AccI GT'mk_AC
Cuts at: 0 210 651
Size: 210 441
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 81 651
Size: 81 570
AhdI GACnn_n'nnGTC
Cuts at: 0 340 651
Size: 340 311
AlwNI CAG_nnn'CTG
Cuts at: 0 454 651
Size: 454 197
ApaI G_GGCC'C
Cuts at: 0 163 191 651
Size: 163 28 460
Fragments arranged by size:
460 163 28
AvaI C'yCGr_G
Cuts at: 0 271 381 651
Size: 271 110 270
Fragments arranged by size:
271 270 110
BanII G_rGCy'C
Cuts at: 0 125 163 191 651
Size: 125 38 28 460
Fragments arranged by size:
460 125 38 28
BbvCI CC'TCA_GC
Cuts at: 0 455 651
Size: 455 196
BciVI GTATCCnnnnn_n'
Cuts at: 0 570 651
Size: 570 81
Bme1580I G_kGCm'C
Cuts at: 0 163 191 651
Size: 163 28 460
Fragments arranged by size:
460 163 28
BmrI ACTGGGnnnn_n'
Cuts at: 0 166 651
Size: 166 485
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 192 304 651
Size: 192 112 347
Fragments arranged by size:
347 192 112
Bpu10I CC'TnA_GC
Cuts at: 0 455 651
Size: 455 196
BsaHI Gr'CG_yC
Cuts at: 0 488 651
Size: 488 163
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 593 623 651
Size: 593 30 28
Fragments arranged by size:
593 30 28
BseYI C'CCAG_C
Cuts at: 0 220 651
Size: 220 431
BsiHKAI G_wGCw'C
Cuts at: 0 125 569 651
Size: 125 444 82
Fragments arranged by size:
444 125 82
BsmBI CGTCTCn'nnnn_
Cuts at: 0 172 651
Size: 172 479
Bsp1286I G_dGCh'C
Cuts at: 0 125 163 191 569 651
Size: 125 38 28 378 82
Fragments arranged by size:
378 125 82 38 28
BsrDI GCAATG_nn'
Cuts at: 0 258 651
Size: 258 393
BsrFI r'CCGG_y
Cuts at: 0 480 651
Size: 480 171
BstEII G'GTnAC_C
Cuts at: 0 6 651
Size: 6 645
BstXI CCAn_nnnn'nTGG
Cuts at: 0 328 651
Size: 328 323
BstYI r'GATC_y
Cuts at: 0 234 421 459 651
Size: 234 187 38 192
Fragments arranged by size:
234 192 187 38
Bsu36I CC'TnA_GG
Cuts at: 0 313 651
Size: 313 338
BtsI GCAGTG_nn'
Cuts at: 0 455 651
Size: 455 196
EaeI y'GGCC_r
Cuts at: 0 408 651
Size: 408 243
EcoICRI GAG'CTC
Cuts at: 0 123 651
Size: 123 528
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 81 192 304 651
Size: 81 111 112 347
Fragments arranged by size:
347 112 111 81
EcoO109I rG'GnC_Cy
Cuts at: 0 187 188 516 651
Size: 187 1 328 135
Fragments arranged by size:
328 187 135 1
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 326 358 651
Size: 326 32 293
Fragments arranged by size:
326 293 32
HincII GTy'rAC
Cuts at: 0 211 486 558 651
Size: 211 275 72 93
Fragments arranged by size:
275 211 93 72
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 264 579 651
Size: 264 315 72
Fragments arranged by size:
315 264 72
MscI TGG'CCA
Cuts at: 0 410 651
Size: 410 241
NspI r_CATG'y
Cuts at: 0 505 651
Size: 505 146
PasI CC'CwG_GG
Cuts at: 0 137 651
Size: 137 514
PpuMI rG'GwC_Cy
Cuts at: 0 516 651
Size: 516 135
PspOMI G'GGCC_C
Cuts at: 0 159 187 651
Size: 159 28 464
Fragments arranged by size:
464 159 28
PstI C_TGCA'G
Cuts at: 0 500 651
Size: 500 151
SacI G_AGCT'C
Cuts at: 0 125 651
Size: 125 526
SalI G'TCGA_C
Cuts at: 0 209 651
Size: 209 442
SfcI C'TryA_G
Cuts at: 0 496 651
Size: 496 155
SmaI CCC'GGG
Cuts at: 0 383 651
Size: 383 268
StyI C'CwwG_G
Cuts at: 0 432 651
Size: 432 219
XmaI C'CCGG_G
Cuts at: 0 381 651
Size: 381 270
ZraI GAC'GTC
Cuts at: 0 489 651
Size: 489 162
Enzymes that do cut:
AatII AccI AcuI AhdI AlwNI ApaI AvaI BanII
BbvCI BciVI Bme1580I BmrI BpmI Bpu10I BsaHI BsaXI
BseYI BsiHKAI BsmBI Bsp1286I BsrDI BsrFI BstEII BstXI
BstYI Bsu36I BtsI EaeI EcoICRI Eco57MI EcoO109I Hin4I
HincII MmeI MscI NspI PasI PpuMI PspOMI PstI
SacI SalI SfcI SmaI StyI XmaI ZraI
Enzymes that do not cut:
AarI Acc65I AclI AfeI AflII AflIII AgeI AleI
AlfI AloI ApaLI ApoI AscI AseI AsiSI AvrII
BaeI BamHI BanI BbeI BbsI BcgI BclI BfrBI
BglI BglII BlpI BmgBI BmtI BplI BpuEI BsaI
BsaAI BsaBI BsaWI BseRI BsgI BsiEI BsiWI BsmI
BspEI BspHI BspMI BsrBI BsrGI BssHII BssSI BstAPI
BstBI BstZ17I BtgI BtgZI ClaI CspCI DraI DraIII
DrdI EagI EarI EciI EcoNI EcoRI EcoRV FalI
FseI FspI FspAI HaeII HindIII HpaI KasI KpnI
MfeI MluI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NsiI PacI PciI PflMI
PfoI PmeI PmlI PpiI PshAI PsiI PspXI PsrI
PvuI PvuII RsrII SacII SanDI SapI SbfI ScaI
SexAI SfiI SfoI SgfI SgrAI SmlI SnaBI SpeI
SphI SrfI SspI StuI SwaI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmnI