(Linear) (Six-base) MAPSORT of: KIBB7097.seq  Check: 6523  from: 1  to: 582



KIBB7097.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 10:58  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     376     582 
   Size:        376     206
 
AflIII A'CryG_T

Cuts at:      0      97     582 
   Size:         97     485
 
BamHI G'GATC_C

Cuts at:      0     329     582 
   Size:        329     253
 
BanI G'GyrC_C

Cuts at:      0     544     582 
   Size:        544      38
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     396     430     582 
   Size:        396      34     152

  Fragments arranged by size: 

                 396     152      34
 
Bme1580I G_kGCm'C

Cuts at:      0     549     582 
   Size:        549      33
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     300     582 
   Size:        300     282
 
BsaAI yAC'GTr

Cuts at:      0      18     582 
   Size:         18     564
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     363     466     567     582 
   Size:        363     103     101      15

  Fragments arranged by size: 

                 363     103     101      15
 
BsiEI CG_ry'CG

Cuts at:      0      12     582 
   Size:         12     570
 
BsiHKAI G_wGCw'C

Cuts at:      0     561     582 
   Size:        561      21
 
Bsp1286I G_dGCh'C

Cuts at:      0     549     561     582 
   Size:        549      12      21

  Fragments arranged by size: 

                 549      21      12
 
BspHI T'CATG_A

Cuts at:      0      53     582 
   Size:         53     529
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     376     582 
   Size:        376     206
 
BsrGI T'GTAC_A

Cuts at:      0     422     582 
   Size:        422     160
 
BssSI C'ACGA_G

Cuts at:      0     161     582 
   Size:        161     421
 
BstYI r'GATC_y

Cuts at:      0     329     582 
   Size:        329     253
 
Bsu36I CC'TnA_GG

Cuts at:      0     202     325     449     523     550     582 
   Size:        202     123     124      74      27      32

  Fragments arranged by size: 

                 202     124     123      74      32      27
 
EaeI y'GGCC_r

Cuts at:      0      20      80     317     582 
   Size:         20      60     237     265

  Fragments arranged by size: 

                 265     237      60      20
 
EarI CTCTTCn'nnn_

Cuts at:      0     403     582 
   Size:        403     179
 
EcoO109I rG'GnC_Cy

Cuts at:      0     465     582 
   Size:        465     117
 
HaeII r_GCGC'y

Cuts at:      0     413     582 
   Size:        413     169
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      32      64     582 
   Size:         32      32     518

  Fragments arranged by size: 

                 518      32      32
 
MscI TGG'CCA

Cuts at:      0      22      82     582 
   Size:         22      60     500

  Fragments arranged by size: 

                 500      60      22
 
MslI CAynn'nnrTG

Cuts at:      0      52     268     418     424     582 
   Size:         52     216     150       6     158

  Fragments arranged by size: 

                 216     158     150      52       6
 
NspI r_CATG'y

Cuts at:      0     101     261     582 
   Size:        101     160     321

  Fragments arranged by size: 

                 321     160     101
 
PciI A'CATG_T

Cuts at:      0      97     582 
   Size:         97     485
 
PfoI T'CCnGG_A

Cuts at:      0      91     582 
   Size:         91     491
 
PpuMI rG'GwC_Cy

Cuts at:      0     465     582 
   Size:        465     117
 
PvuI CG_AT'CG

Cuts at:      0      12     582 
   Size:         12     570
 
SexAI A'CCwGG_T

Cuts at:      0     233     582 
   Size:        233     349
 
StuI AGG'CCT

Cuts at:      0     358     582 
   Size:        358     224
 
TatI w'GTAC_w

Cuts at:      0     422     582 
   Size:        422     160
 

 Enzymes that do cut:

     AarI   AflIII    BamHI     BanI     BcgI Bme1580I     BsaI    BsaAI
    BseRI    BsiEI  BsiHKAI Bsp1286I    BspHI    BspMI    BsrGI    BssSI
    BstYI   Bsu36I     EaeI     EarI EcoO109I    HaeII    Hin4I     MscI
     MslI     NspI     PciI     PfoI    PpuMI     PvuI    SexAI     StuI
     TatI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII     AgeI
     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     ApoI
     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BanII     BbeI
     BbsI    BbvCI    BciVI     BclI    BfrBI     BglI    BglII     BlpI
    BmgBI     BmrI     BmtI     BplI     BpmI   Bpu10I    BpuEI    BsaBI
    BsaHI    BsaWI    BsaXI    BseYI     BsgI    BsiWI     BsmI    BsmBI
    BspEI    BsrBI    BsrDI    BsrFI   BssHII   BstAPI    BstBI   BstEII
    BstXI  BstZ17I     BtgI    BtgZI     BtsI     ClaI    CspCI     DraI
   DraIII     DrdI     EagI     EciI  EcoICRI  Eco57MI    EcoNI    EcoRI
    EcoRV     FalI     FseI     FspI    FspAI   HincII  HindIII     HpaI
     KasI     KpnI     MfeI     MluI     MmeI   MspA1I     NaeI     NarI
     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     PacI
     PasI    PflMI     PmeI     PmlI     PpiI    PshAI     PsiI   PspOMI
    PspXI     PsrI     PstI    PvuII    RsrII     SacI    SacII     SalI
    SanDI     SapI     SbfI     ScaI     SfcI     SfiI     SfoI     SgfI
    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI     SspI
     StyI     SwaI    TaqII     TstI  Tth111I     XbaI     XcmI     XhoI
     XmaI     XmnI     ZraI