(Linear) (Six-base) MAPSORT of: KIBB7095.seq  Check: 8431  from: 1  to: 564



KIBB7095.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 10:58  ..

Acc65I G'GTAC_C

Cuts at:      0     204     564 
   Size:        204     360
 
AflIII A'CryG_T

Cuts at:      0     392     564 
   Size:        392     172
 
AleI CACnn'nnGTG

Cuts at:      0      12     564 
   Size:         12     552
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     329     564 
   Size:        329     235
 
ApaLI G'TGCA_C

Cuts at:      0     490     564 
   Size:        490      74
 
BanI G'GyrC_C

Cuts at:      0     204     564 
   Size:        204     360
 
BlpI GC'TnA_GC

Cuts at:      0     330     564 
   Size:        330     234
 
Bme1580I G_kGCm'C

Cuts at:      0     494     564 
   Size:        494      70
 
BmgBI CAC'GTC

Cuts at:      0     232     564 
   Size:        232     332
 
BmrI ACTGGGnnnn_n'

Cuts at:      0      19     502     564 
   Size:         19     483      62

  Fragments arranged by size: 

                 483      62      19
 
BsaAI yAC'GTr

Cuts at:      0     395     564 
   Size:        395     169
 
BsaHI Gr'CG_yC

Cuts at:      0     386     564 
   Size:        386     178
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     289     564 
   Size:        289     275
 
BsiHKAI G_wGCw'C

Cuts at:      0     494     564 
   Size:        494      70
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     238     564 
   Size:        238     326
 
Bsp1286I G_dGCh'C

Cuts at:      0     494     564 
   Size:        494      70
 
BspHI T'CATG_A

Cuts at:      0     356     564 
   Size:        356     208
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     192     564 
   Size:        192     372
 
BsrBI CCG'CTC

Cuts at:      0     329     564 
   Size:        329     235
 
BsrDI GCAATG_nn'

Cuts at:      0      96     564 
   Size:         96     468
 
BsrFI r'CCGG_y

Cuts at:      0     410     564 
   Size:        410     154
 
BssSI C'ACGA_G

Cuts at:      0      12     564 
   Size:         12     552
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     475     564 
   Size:        475      89
 
BtgI C'CryG_G

Cuts at:      0     140     564 
   Size:        140     424
 
DraIII CAC_nnn'GTG

Cuts at:      0     276     564 
   Size:        276     288
 
EaeI y'GGCC_r

Cuts at:      0      98     564 
   Size:         98     466
 
EcoRV GAT'ATC

Cuts at:      0     189     564 
   Size:        189     375
 
KpnI G_GTAC'C

Cuts at:      0     208     564 
   Size:        208     356
 
MfeI C'AATT_G

Cuts at:      0     469     564 
   Size:        469      95
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     447     564 
   Size:        447     117
 
MslI CAynn'nnrTG

Cuts at:      0      12     564 
   Size:         12     552
 
MspA1I CmG'CkG

Cuts at:      0      53     334     434     564 
   Size:         53     281     100     130

  Fragments arranged by size: 

                 281     130     100      53
 
NaeI GCC'GGC

Cuts at:      0     412     564 
   Size:        412     152
 
NcoI C'CATG_G

Cuts at:      0     140     564 
   Size:        140     424
 
NgoMIV G'CCGG_C

Cuts at:      0     410     564 
   Size:        410     154
 
NspI r_CATG'y

Cuts at:      0     201     564 
   Size:        201     363
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     276     564 
   Size:        276     288
 
PmlI CAC'GTG

Cuts at:      0     395     564 
   Size:        395     169
 
PvuII CAG'CTG

Cuts at:      0     334     564 
   Size:        334     230
 
StyI C'CwwG_G

Cuts at:      0     140     564 
   Size:        140     424
 
TatI w'GTAC_w

Cuts at:      0     194     564 
   Size:        194     370
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     358     390     564 
   Size:        358      32     174

  Fragments arranged by size: 

                 358     174      32
 
Tth111I GACn'n_nGTC

Cuts at:      0     402     564 
   Size:        402     162
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     378     564 
   Size:        378     186
 

 Enzymes that do cut:

   Acc65I   AflIII     AleI     AlfI    ApaLI     BanI     BlpI Bme1580I
    BmgBI     BmrI    BsaAI    BsaHI     BsgI  BsiHKAI    BsmBI Bsp1286I
    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BssSI    BstXI     BtgI
   DraIII     EaeI    EcoRV     KpnI     MfeI     MmeI     MslI   MspA1I
     NaeI     NcoI   NgoMIV     NspI    PflMI     PmlI    PvuII     StyI
     TatI     TstI  Tth111I     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AccI     AclI     AcuI     AfeI    AflII     AgeI
     AhdI     AloI    AlwNI     ApaI     ApoI     AscI     AseI    AsiSI
     AvaI    AvrII     BaeI    BamHI    BanII     BbeI     BbsI    BbvCI
     BcgI    BciVI     BclI    BfrBI     BglI    BglII     BmtI     BplI
     BpmI   Bpu10I    BpuEI     BsaI    BsaBI    BsaWI    BsaXI    BseRI
    BseYI    BsiEI    BsiWI     BsmI    BspEI    BsrGI   BssHII   BstAPI
    BstBI   BstEII    BstYI  BstZ17I   Bsu36I    BtgZI     BtsI     ClaI
    CspCI     DraI     DrdI     EagI     EarI     EciI  EcoICRI  Eco57MI
    EcoNI EcoO109I    EcoRI     FalI     FseI     FspI    FspAI    HaeII
    Hin4I   HincII  HindIII     HpaI     KasI     MluI     MscI     NarI
     NdeI     NheI     NotI     NruI     NsiI     PacI     PasI     PciI
     PfoI     PmeI     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI
     PsrI     PstI     PvuI    RsrII     SacI    SacII     SalI    SanDI
     SapI     SbfI     ScaI    SexAI     SfcI     SfiI     SfoI     SgfI
    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI     SspI
     StuI     SwaI    TaqII     XbaI     XhoI     XmaI     XmnI     ZraI