Restriction map for
pF1KB7094
(Linear) (Six-base) MAPSORT of: KIBB7094.seq Check: 2330 from: 1 to: 558
KIBB7094.orf
With 182 enzymes: SgfI *
March 3, 2008 10:58 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 515 558
Size: 515 43
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 223 558
Size: 223 335
AflIII A'CryG_T
Cuts at: 0 434 558
Size: 434 124
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 127 558
Size: 127 431
AlwNI CAG_nnn'CTG
Cuts at: 0 518 558
Size: 518 40
AvaI C'yCGr_G
Cuts at: 0 361 558
Size: 361 197
BanII G_rGCy'C
Cuts at: 0 494 558
Size: 494 64
BglI GCCn_nnn'nGGC
Cuts at: 0 298 558
Size: 298 260
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 44 558
Size: 44 514
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 163 558
Size: 163 395
BsaAI yAC'GTr
Cuts at: 0 435 558
Size: 435 123
BsaHI Gr'CG_yC
Cuts at: 0 64 558
Size: 64 494
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 259 558
Size: 259 299
BseYI C'CCAG_C
Cuts at: 0 207 234 558
Size: 207 27 324
Fragments arranged by size:
324 207 27
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 364 558
Size: 364 194
BsiEI CG_ry'CG
Cuts at: 0 302 336 423 558
Size: 302 34 87 135
Fragments arranged by size:
302 135 87 34
Bsp1286I G_dGCh'C
Cuts at: 0 494 558
Size: 494 64
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 383 515 558
Size: 383 132 43
Fragments arranged by size:
383 132 43
BsrBI CCG'CTC
Cuts at: 0 500 558
Size: 500 58
BsrFI r'CCGG_y
Cuts at: 0 335 558
Size: 335 223
BssHII G'CGCG_C
Cuts at: 0 280 558
Size: 280 278
BstAPI GCAn_nnn'nTGC
Cuts at: 0 518 558
Size: 518 40
BstXI CCAn_nnnn'nTGG
Cuts at: 0 24 75 558
Size: 24 51 483
Fragments arranged by size:
483 51 24
BstYI r'GATC_y
Cuts at: 0 515 558
Size: 515 43
BtgI C'CryG_G
Cuts at: 0 389 417 558
Size: 389 28 141
Fragments arranged by size:
389 141 28
BtsI GCAGTG_nn'
Cuts at: 0 80 111 558
Size: 80 31 447
Fragments arranged by size:
447 80 31
DraIII CAC_nnn'GTG
Cuts at: 0 318 558
Size: 318 240
DrdI GACnn_nn'nnGTC
Cuts at: 0 411 558
Size: 411 147
EaeI y'GGCC_r
Cuts at: 0 80 290 299 347 420 441 558
Size: 80 210 9 48 73 21 117
Fragments arranged by size:
210 117 80 73 48 21 9
EagI C'GGCC_G
Cuts at: 0 299 420 558
Size: 299 121 138
Fragments arranged by size:
299 138 121
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 259 558
Size: 259 299
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 223 558
Size: 223 335
EcoNI CCTnn'n_nnAGG
Cuts at: 0 190 558
Size: 190 368
EcoO109I rG'GnC_Cy
Cuts at: 0 394 525 558
Size: 394 131 33
Fragments arranged by size:
394 131 33
FspI TGC'GCA
Cuts at: 0 511 558
Size: 511 47
HaeII r_GCGC'y
Cuts at: 0 256 267 558
Size: 256 11 291
Fragments arranged by size:
291 256 11
MscI TGG'CCA
Cuts at: 0 292 558
Size: 292 266
MspA1I CmG'CkG
Cuts at: 0 234 558
Size: 234 324
PflMI CCAn_nnn'nTGG
Cuts at: 0 318 390 558
Size: 318 72 168
Fragments arranged by size:
318 168 72
PmlI CAC'GTG
Cuts at: 0 435 558
Size: 435 123
PpuMI rG'GwC_Cy
Cuts at: 0 394 558
Size: 394 164
PshAI GACnn'nnGTC
Cuts at: 0 301 558
Size: 301 257
PspXI vC'TCGA_Gb
Cuts at: 0 361 558
Size: 361 197
PstI C_TGCA'G
Cuts at: 0 35 558
Size: 35 523
PvuII CAG'CTG
Cuts at: 0 234 558
Size: 234 324
SanDI GG'GwC_CC
Cuts at: 0 394 558
Size: 394 164
SexAI A'CCwGG_T
Cuts at: 0 185 314 558
Size: 185 129 244
Fragments arranged by size:
244 185 129
SfcI C'TryA_G
Cuts at: 0 31 558
Size: 31 527
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 298 558
Size: 298 260
SmlI C'TyrA_G
Cuts at: 0 178 361 558
Size: 178 183 197
Fragments arranged by size:
197 183 178
StyI C'CwwG_G
Cuts at: 0 68 558
Size: 68 490
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 75 558
Size: 75 483
XhoI C'TCGA_G
Cuts at: 0 361 558
Size: 361 197
Enzymes that do cut:
AarI AcuI AflIII AlfI AlwNI AvaI BanII BglI
BplI BpuEI BsaAI BsaHI BseRI BseYI BsgI BsiEI
Bsp1286I BspMI BsrBI BsrFI BssHII BstAPI BstXI BstYI
BtgI BtsI DraIII DrdI EaeI EagI EciI Eco57MI
EcoNI EcoO109I FspI HaeII MscI MspA1I PflMI PmlI
PpuMI PshAI PspXI PstI PvuII SanDI SexAI SfcI
SfiI SmlI StyI XcmI XhoI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AgeI AhdI
AleI AloI ApaI ApaLI ApoI AscI AseI AsiSI
AvrII BaeI BamHI BanI BbeI BbsI BbvCI BcgI
BciVI BclI BfrBI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BsaI BsaBI BsaWI BsaXI BsiHKAI
BsiWI BsmI BsmBI BspEI BspHI BsrDI BsrGI BssSI
BstBI BstEII BstZ17I Bsu36I BtgZI ClaI CspCI DraI
EarI EcoICRI EcoRI EcoRV FalI FseI FspAI Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MslI NaeI NarI NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspI PacI PasI PciI PfoI PmeI
PpiI PsiI PspOMI PsrI PvuI RsrII SacI SacII
SalI SapI SbfI ScaI SfoI SgfI SgrAI SmaI
SnaBI SpeI SphI SrfI SspI StuI SwaI TaqII
TatI TstI Tth111I XbaI XmaI XmnI ZraI