Restriction map for
pF1KB7092
(Linear) (Six-base) MAPSORT of: KIBB7092.seq Check: 6534 from: 1 to: 552
KIBB7092.orf
With 182 enzymes: SgfI *
March 3, 2008 10:58 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 33 552
Size: 33 519
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 58 355 552
Size: 58 297 197
Fragments arranged by size:
297 197 58
AflII C'TTAA_G
Cuts at: 0 13 552
Size: 13 539
AflIII A'CryG_T
Cuts at: 0 408 552
Size: 408 144
AleI CACnn'nnGTG
Cuts at: 0 478 552
Size: 478 74
AlwNI CAG_nnn'CTG
Cuts at: 0 33 552
Size: 33 519
ApaI G_GGCC'C
Cuts at: 0 418 552
Size: 418 134
BanI G'GyrC_C
Cuts at: 0 179 264 552
Size: 179 85 288
Fragments arranged by size:
288 179 85
BanII G_rGCy'C
Cuts at: 0 290 418 552
Size: 290 128 134
Fragments arranged by size:
290 134 128
BbeI G_GCGC'C
Cuts at: 0 183 552
Size: 183 369
BlpI GC'TnA_GC
Cuts at: 0 151 552
Size: 151 401
Bme1580I G_kGCm'C
Cuts at: 0 269 404 418 552
Size: 269 135 14 134
Fragments arranged by size:
269 135 134 14
BmgBI CAC'GTC
Cuts at: 0 423 552
Size: 423 129
BmrI ACTGGGnnnn_n'
Cuts at: 0 69 552
Size: 69 483
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 304 552
Size: 304 248
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 331 552
Size: 331 221
BsaAI yAC'GTr
Cuts at: 0 405 507 552
Size: 405 102 45
Fragments arranged by size:
405 102 45
BsaHI Gr'CG_yC
Cuts at: 0 180 552
Size: 180 372
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 334 538 552
Size: 334 204 14
Fragments arranged by size:
334 204 14
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 67 552
Size: 67 485
BsiEI CG_ry'CG
Cuts at: 0 230 552
Size: 230 322
BsiHKAI G_wGCw'C
Cuts at: 0 290 344 552
Size: 290 54 208
Fragments arranged by size:
290 208 54
BsiWI C'GTAC_G
Cuts at: 0 405 552
Size: 405 147
Bsp1286I G_dGCh'C
Cuts at: 0 269 290 344 404 418 552
Size: 269 21 54 60 14 134
Fragments arranged by size:
269 134 60 54 21 14
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 33 89 200 552
Size: 33 56 111 352
Fragments arranged by size:
352 111 56 33
BsrFI r'CCGG_y
Cuts at: 0 110 552
Size: 110 442
BssHII G'CGCG_C
Cuts at: 0 219 552
Size: 219 333
BssSI C'ACGA_G
Cuts at: 0 22 85 552
Size: 22 63 467
Fragments arranged by size:
467 63 22
BstAPI GCAn_nnn'nTGC
Cuts at: 0 33 90 552
Size: 33 57 462
Fragments arranged by size:
462 57 33
BstEII G'GTnAC_C
Cuts at: 0 66 426 552
Size: 66 360 126
Fragments arranged by size:
360 126 66
BtgI C'CryG_G
Cuts at: 0 380 552
Size: 380 172
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 545 552
Size: 545 7
EaeI y'GGCC_r
Cuts at: 0 227 552
Size: 227 325
EagI C'GGCC_G
Cuts at: 0 227 552
Size: 227 325
EcoICRI GAG'CTC
Cuts at: 0 288 552
Size: 288 264
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 58 304 355 552
Size: 58 246 51 197
Fragments arranged by size:
246 197 58 51
EcoO109I rG'GnC_Cy
Cuts at: 0 415 552
Size: 415 137
FspI TGC'GCA
Cuts at: 0 355 552
Size: 355 197
HaeII r_GCGC'y
Cuts at: 0 183 552
Size: 183 369
HindIII A'AGCT_T
Cuts at: 0 10 552
Size: 10 542
KasI G'GCGC_C
Cuts at: 0 179 552
Size: 179 373
MluI A'CGCG_T
Cuts at: 0 408 552
Size: 408 144
MslI CAynn'nnrTG
Cuts at: 0 478 552
Size: 478 74
MspA1I CmG'CkG
Cuts at: 0 120 150 552
Size: 120 30 402
Fragments arranged by size:
402 120 30
NarI GG'CG_CC
Cuts at: 0 180 552
Size: 180 372
PmlI CAC'GTG
Cuts at: 0 507 552
Size: 507 45
PspOMI G'GGCC_C
Cuts at: 0 414 552
Size: 414 138
PstI C_TGCA'G
Cuts at: 0 119 552
Size: 119 433
PvuII CAG'CTG
Cuts at: 0 150 552
Size: 150 402
SacI G_AGCT'C
Cuts at: 0 290 552
Size: 290 262
SfcI C'TryA_G
Cuts at: 0 115 552
Size: 115 437
SfoI GGC'GCC
Cuts at: 0 181 552
Size: 181 371
SmlI C'TyrA_G
Cuts at: 0 13 346 552
Size: 13 333 206
Fragments arranged by size:
333 206 13
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 468 552
Size: 468 84
TatI w'GTAC_w
Cuts at: 0 515 552
Size: 515 37
Tth111I GACn'n_nGTC
Cuts at: 0 298 469 552
Size: 298 171 83
Fragments arranged by size:
298 171 83
Enzymes that do cut:
AarI AcuI AflII AflIII AleI AlwNI ApaI BanI
BanII BbeI BlpI Bme1580I BmgBI BmrI BpmI BpuEI
BsaAI BsaHI BseRI BsgI BsiEI BsiHKAI BsiWI Bsp1286I
BspMI BsrFI BssHII BssSI BstAPI BstEII BtgI BtgZI
EaeI EagI EcoICRI Eco57MI EcoO109I FspI HaeII HindIII
KasI MluI MslI MspA1I NarI PmlI PspOMI PstI
PvuII SacI SfcI SfoI SmlI TaqII TatI Tth111I
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AgeI AhdI AlfI
AloI ApaLI ApoI AscI AseI AsiSI AvaI AvrII
BaeI BamHI BbsI BbvCI BcgI BciVI BclI BfrBI
BglI BglII BmtI BplI Bpu10I BsaI BsaBI BsaWI
BsaXI BseYI BsmI BsmBI BspEI BspHI BsrBI BsrDI
BsrGI BstBI BstXI BstYI BstZ17I Bsu36I BtsI ClaI
CspCI DraI DraIII DrdI EarI EciI EcoNI EcoRI
EcoRV FalI FseI FspAI Hin4I HincII HpaI KpnI
MfeI MmeI MscI NaeI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspI PacI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PsiI PspXI PsrI
PvuI RsrII SacII SalI SanDI SapI SbfI ScaI
SexAI SfiI SgfI SgrAI SmaI SnaBI SpeI SphI
SrfI SspI StuI StyI SwaI TstI XbaI XcmI
XhoI XmaI XmnI ZraI