(Linear) (Six-base) MAPSORT of: KIBB7091.seq  Check: 5489  from: 1  to: 552



KIBB7091.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 10:58  ..

AccI GT'mk_AC

Cuts at:      0     320     552 
   Size:        320     232
 
AflIII A'CryG_T

Cuts at:      0     434     552 
   Size:        434     118
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     127     552 
   Size:        127     425
 
AlwNI CAG_nnn'CTG

Cuts at:      0     279     552 
   Size:        279     273
 
BanI G'GyrC_C

Cuts at:      0     289     552 
   Size:        289     263
 
BanII G_rGCy'C

Cuts at:      0     125     552 
   Size:        125     427
 
BclI T'GATC_A

Cuts at:      0     176     188     441     552 
   Size:        176      12     253     111

  Fragments arranged by size: 

                 253     176     111      12
 
BglI GCCn_nnn'nGGC

Cuts at:      0     298     552 
   Size:        298     254
 
BglII A'GATC_T

Cuts at:      0     509     552 
   Size:        509      43
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     236     552 
   Size:        236     316
 
BsaAI yAC'GTr

Cuts at:      0     435     552 
   Size:        435     117
 
BseYI C'CCAG_C

Cuts at:      0     167     552 
   Size:        167     385
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     202     552 
   Size:        202     350
 
BsiHKAI G_wGCw'C

Cuts at:      0     125     552 
   Size:        125     427
 
Bsp1286I G_dGCh'C

Cuts at:      0     125     552 
   Size:        125     427
 
BsrDI GCAATG_nn'

Cuts at:      0     231     552 
   Size:        231     321
 
BsrFI r'CCGG_y

Cuts at:      0     155     552 
   Size:        155     397
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     207     249     552 
   Size:        207      42     303

  Fragments arranged by size: 

                 303     207      42
 
BstYI r'GATC_y

Cuts at:      0     509     552 
   Size:        509      43
 
BtsI GCAGTG_nn'

Cuts at:      0      28     111     552 
   Size:         28      83     441

  Fragments arranged by size: 

                 441      83      28
 
EaeI y'GGCC_r

Cuts at:      0     197     420     552 
   Size:        197     223     132

  Fragments arranged by size: 

                 223     197     132
 
EarI CTCTTCn'nnn_

Cuts at:      0      91     552 
   Size:         91     461
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     418     552 
   Size:        418     134
 
EcoICRI GAG'CTC

Cuts at:      0     123     552 
   Size:        123     429
 
EcoO109I rG'GnC_Cy

Cuts at:      0     183     552 
   Size:        183     369
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      51      83     433     465     552 
   Size:         51      32     350      32      87

  Fragments arranged by size: 

                 350      87      51      32      32
 
MscI TGG'CCA

Cuts at:      0     199     552 
   Size:        199     353
 
MslI CAynn'nnrTG

Cuts at:      0     205     224     552 
   Size:        205      19     328

  Fragments arranged by size: 

                 328     205      19
 
NaeI GCC'GGC

Cuts at:      0     157     552 
   Size:        157     395
 
NgoMIV G'CCGG_C

Cuts at:      0     155     552 
   Size:        155     397
 
PmlI CAC'GTG

Cuts at:      0     435     552 
   Size:        435     117
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     386     418     552 
   Size:        386      32     134

  Fragments arranged by size: 

                 386     134      32
 
PpuMI rG'GwC_Cy

Cuts at:      0     183     552 
   Size:        183     369
 
PshAI GACnn'nnGTC

Cuts at:      0     458     552 
   Size:        458      94
 
SacI G_AGCT'C

Cuts at:      0     125     552 
   Size:        125     427
 
StyI C'CwwG_G

Cuts at:      0     137     501     552 
   Size:        137     364      51

  Fragments arranged by size: 

                 364     137      51
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     376     552 
   Size:        376     176
 
TatI w'GTAC_w

Cuts at:      0     143     552 
   Size:        143     409
 

 Enzymes that do cut:

     AccI   AflIII     AlfI    AlwNI     BanI    BanII     BclI     BglI
    BglII     BmrI    BsaAI    BseYI     BsgI  BsiHKAI Bsp1286I    BsrDI
    BsrFI    BstXI    BstYI     BtsI     EaeI     EarI     EciI  EcoICRI
 EcoO109I    Hin4I     MscI     MslI     NaeI   NgoMIV     PmlI     PpiI
    PpuMI    PshAI     SacI     StyI    TaqII     TatI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AcuI     AfeI    AflII     AgeI
     AhdI     AleI     AloI     ApaI    ApaLI     ApoI     AscI     AseI
    AsiSI     AvaI    AvrII     BaeI    BamHI     BbeI     BbsI    BbvCI
     BcgI    BciVI    BfrBI     BlpI Bme1580I    BmgBI     BmtI     BplI
     BpmI   Bpu10I    BpuEI     BsaI    BsaBI    BsaHI    BsaWI    BsaXI
    BseRI    BsiEI    BsiWI     BsmI    BsmBI    BspEI    BspHI    BspMI
    BsrBI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII  BstZ17I
   Bsu36I     BtgI    BtgZI     ClaI    CspCI     DraI   DraIII     DrdI
     EagI  Eco57MI    EcoNI    EcoRI    EcoRV     FalI     FseI     FspI
    FspAI    HaeII   HincII  HindIII     HpaI     KasI     KpnI     MfeI
     MluI     MmeI   MspA1I     NarI     NcoI     NdeI     NheI     NotI
     NruI     NsiI     NspI     PacI     PasI     PciI    PflMI     PfoI
     PmeI     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI    PvuII
    RsrII    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI
     SfcI     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI
     SpeI     SphI     SrfI     SspI     StuI     SwaI     TstI  Tth111I
     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI