(Linear) (Six-base) MAPSORT of: KIBB7089.seq  Check: 2307  from: 1  to: 456



KIBB7089.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 10:58  ..

AflIII A'CryG_T

Cuts at:      0     200     456 
   Size:        200     256
 
BanII G_rGCy'C

Cuts at:      0     410     419     456 
   Size:        410       9      37

  Fragments arranged by size: 

                 410      37       9
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     310     344     456 
   Size:        310      34     112

  Fragments arranged by size: 

                 310     112      34
 
BglI GCCn_nnn'nGGC

Cuts at:      0     423     456 
   Size:        423      33
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     405     456 
   Size:        405      51
 
Bpu10I CC'TnA_GC

Cuts at:      0     106     456 
   Size:        106     350
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     151     456 
   Size:        151     305
 
BsaAI yAC'GTr

Cuts at:      0     201     456 
   Size:        201     255
 
BsaHI Gr'CG_yC

Cuts at:      0     374     456 
   Size:        374      82
 
BsiEI CG_ry'CG

Cuts at:      0     102     392     456 
   Size:        102     290      64

  Fragments arranged by size: 

                 290     102      64
 
BsiHKAI G_wGCw'C

Cuts at:      0      92     456 
   Size:         92     364
 
Bsp1286I G_dGCh'C

Cuts at:      0      92     410     419     456 
   Size:         92     318       9      37

  Fragments arranged by size: 

                 318      92      37       9
 
BssHII G'CGCG_C

Cuts at:      0     131     133     456 
   Size:        131       2     323

  Fragments arranged by size: 

                 323     131       2
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     285     456 
   Size:        285     171
 
BtgI C'CryG_G

Cuts at:      0     377     386     456 
   Size:        377       9      70

  Fragments arranged by size: 

                 377      70       9
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     264     456 
   Size:        264     192
 
ClaI AT'CG_AT

Cuts at:      0     296     456 
   Size:        296     160
 
EaeI y'GGCC_r

Cuts at:      0     275     389     456 
   Size:        275     114      67

  Fragments arranged by size: 

                 275     114      67
 
EagI C'GGCC_G

Cuts at:      0     389     456 
   Size:        389      67
 
EarI CTCTTCn'nnn_

Cuts at:      0      64     456 
   Size:         64     392
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     405     456 
   Size:        405      51
 
HaeII r_GCGC'y

Cuts at:      0     292     456 
   Size:        292     164
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0      17     122     456 
   Size:         17     105     334

  Fragments arranged by size: 

                 334     105      17
 
MspA1I CmG'CkG

Cuts at:      0     248     388     456 
   Size:        248     140      68

  Fragments arranged by size: 

                 248     140      68
 
NotI GC'GGCC_GC

Cuts at:      0     389     456 
   Size:        389      67
 
PstI C_TGCA'G

Cuts at:      0     323     456 
   Size:        323     133
 
SacII CC_GC'GG

Cuts at:      0     389     456 
   Size:        389      67
 
SbfI CC_TGCA'GG

Cuts at:      0     323     456 
   Size:        323     133
 
SfcI C'TryA_G

Cuts at:      0     319     456 
   Size:        319     137
 
SmlI C'TyrA_G

Cuts at:      0     166     456 
   Size:        166     290
 
StyI C'CwwG_G

Cuts at:      0     140     456 
   Size:        140     316
 
TatI w'GTAC_w

Cuts at:      0     203     456 
   Size:        203     253
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     168     456 
   Size:        168     288
 

 Enzymes that do cut:

   AflIII    BanII     BcgI     BglI     BpmI   Bpu10I    BpuEI    BsaAI
    BsaHI    BsiEI  BsiHKAI Bsp1286I   BssHII    BstXI     BtgI    BtgZI
     ClaI     EaeI     EagI     EarI  Eco57MI    HaeII     MmeI   MspA1I
     NotI     PstI    SacII     SbfI     SfcI     SmlI     StyI     TatI
     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI
     ApoI     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI
     BanI     BbeI     BbsI    BbvCI    BciVI     BclI    BfrBI    BglII
     BlpI Bme1580I    BmgBI     BmrI     BmtI     BplI     BsaI    BsaBI
    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiWI     BsmI    BsmBI
    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI    BssSI
   BstAPI    BstBI   BstEII    BstYI  BstZ17I   Bsu36I     BtsI    CspCI
     DraI   DraIII     DrdI     EciI  EcoICRI    EcoNI EcoO109I    EcoRI
    EcoRV     FalI     FseI     FspI    FspAI    Hin4I   HincII  HindIII
     HpaI     KasI     KpnI     MfeI     MluI     MscI     MslI     NaeI
     NarI     NcoI     NdeI   NgoMIV     NheI     NruI     NsiI     NspI
     PacI     PasI     PciI    PflMI     PfoI     PmeI     PmlI     PpiI
    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI     PvuI    PvuII
    RsrII     SacI     SalI    SanDI     SapI     ScaI    SexAI     SfiI
     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI
     SspI     StuI     SwaI    TaqII     TstI  Tth111I     XbaI     XhoI
     XmaI     XmnI     ZraI