(Linear) (Six-base) MAPSORT of: KIBB7086.seq  Check: 4935  from: 1  to: 399



KIBB7086.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 10:58  ..

Acc65I G'GTAC_C

Cuts at:      0     236     399 
   Size:        236     163
 
AflII C'TTAA_G

Cuts at:      0     256     399 
   Size:        256     143
 
BanI G'GyrC_C

Cuts at:      0     236     399 
   Size:        236     163
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     318     399 
   Size:        318      81
 
BclI T'GATC_A

Cuts at:      0      59     399 
   Size:         59     340
 
BglI GCCn_nnn'nGGC

Cuts at:      0     349     399 
   Size:        349      50
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0      46     399 
   Size:         46     353
 
BseYI C'CCAG_C

Cuts at:      0     172     352     399 
   Size:        172     180      47

  Fragments arranged by size: 

                 180     172      47
 
BsrDI GCAATG_nn'

Cuts at:      0     242     254     399 
   Size:        242      12     145

  Fragments arranged by size: 

                 242     145      12
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     283     399 
   Size:        283     116
 
DraIII CAC_nnn'GTG

Cuts at:      0     283     399 
   Size:        283     116
 
EarI CTCTTCn'nnn_

Cuts at:      0      58     399 
   Size:         58     341
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     207     282     399 
   Size:        207      75     117

  Fragments arranged by size: 

                 207     117      75
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     365     397     399 
   Size:        365      32       2

  Fragments arranged by size: 

                 365      32       2
 
KpnI G_GTAC'C

Cuts at:      0     240     399 
   Size:        240     159
 
MslI CAynn'nnrTG

Cuts at:      0      67     247     399 
   Size:         67     180     152

  Fragments arranged by size: 

                 180     152      67
 
ScaI AGT'ACT

Cuts at:      0      26     399 
   Size:         26     373
 
SmlI C'TyrA_G

Cuts at:      0     256     399 
   Size:        256     143
 
SspI AAT'ATT

Cuts at:      0     219     399 
   Size:        219     180
 
StyI C'CwwG_G

Cuts at:      0      11      94     399 
   Size:         11      83     305

  Fragments arranged by size: 

                 305      83      11
 
TatI w'GTAC_w

Cuts at:      0      24     399 
   Size:         24     375
 
XmnI GAAnn'nnTTC

Cuts at:      0     103     399 
   Size:        103     296
 

 Enzymes that do cut:

   Acc65I    AflII     BanI     BbsI     BclI     BglI     BplI    BseYI
    BsrDI   BstAPI   DraIII     EarI     FalI    Hin4I     KpnI     MslI
     ScaI     SmlI     SspI     StyI     TatI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI     AclI     AcuI     AfeI   AflIII     AgeI
     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     ApoI
     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI    BanII
     BbeI    BbvCI     BcgI    BciVI    BfrBI    BglII     BlpI Bme1580I
    BmgBI     BmrI     BmtI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI
    BsaBI    BsaHI    BsaWI    BsaXI    BseRI     BsgI    BsiEI  BsiHKAI
    BsiWI     BsmI    BsmBI Bsp1286I    BspEI    BspHI    BspMI    BsrBI
    BsrFI    BsrGI   BssHII    BssSI    BstBI   BstEII    BstXI    BstYI
  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI     ClaI    CspCI     DraI
     DrdI     EaeI     EagI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I
    EcoRI    EcoRV     FseI     FspI    FspAI    HaeII   HincII  HindIII
     HpaI     KasI     MfeI     MluI     MmeI     MscI   MspA1I     NaeI
     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI
     NspI     PacI     PasI     PciI    PflMI     PfoI     PmeI     PmlI
     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI
     PvuI    PvuII    RsrII     SacI    SacII     SalI    SanDI     SapI
     SbfI    SexAI     SfcI     SfiI     SfoI     SgfI    SgrAI     SmaI
    SnaBI     SpeI     SphI     SrfI     StuI     SwaI    TaqII     TstI
  Tth111I     XbaI     XcmI     XhoI     XmaI     ZraI