(Linear) (Six-base) MAPSORT of: KIBB7083.seq  Check: 3789  from: 1  to: 378



KIBB7083.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 10:58  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     157     378 
   Size:        157     221
 
AfeI AGC'GCT

Cuts at:      0     259     378 
   Size:        259     119
 
ApoI r'AATT_y

Cuts at:      0     189     378 
   Size:        189     189
 
BanI G'GyrC_C

Cuts at:      0     343     378 
   Size:        343      35
 
BanII G_rGCy'C

Cuts at:      0      43     378 
   Size:         43     335
 
Bme1580I G_kGCm'C

Cuts at:      0     346     378 
   Size:        346      32
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     258     378 
   Size:        258     120
 
BsiEI CG_ry'CG

Cuts at:      0     293     378 
   Size:        293      85
 
Bsp1286I G_dGCh'C

Cuts at:      0      43     346     378 
   Size:         43     303      32

  Fragments arranged by size: 

                 303      43      32
 
BspHI T'CATG_A

Cuts at:      0     185     378 
   Size:        185     193
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     132     378 
   Size:        132     246
 
BsrDI GCAATG_nn'

Cuts at:      0     182     378 
   Size:        182     196
 
BsrFI r'CCGG_y

Cuts at:      0     157     378 
   Size:        157     221
 
BsrGI T'GTAC_A

Cuts at:      0     125     378 
   Size:        125     253
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     177     378 
   Size:        177     201
 
BstYI r'GATC_y

Cuts at:      0     281     378 
   Size:        281      97
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     204     378 
   Size:        204     174
 
DraIII CAC_nnn'GTG

Cuts at:      0     333     378 
   Size:        333      45
 
EaeI y'GGCC_r

Cuts at:      0     290     320     378 
   Size:        290      30      58

  Fragments arranged by size: 

                 290      58      30
 
EagI C'GGCC_G

Cuts at:      0     290     378 
   Size:        290      88
 
EarI CTCTTCn'nnn_

Cuts at:      0     171     378 
   Size:        171     207
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     157     258     378 
   Size:        157     101     120

  Fragments arranged by size: 

                 157     120     101
 
EcoRI G'AATT_C

Cuts at:      0     189     378 
   Size:        189     189
 
HaeII r_GCGC'y

Cuts at:      0     261     378 
   Size:        261     117
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      48      80     378 
   Size:         48      32     298

  Fragments arranged by size: 

                 298      48      32
 
HincII GTy'rAC

Cuts at:      0     201     378 
   Size:        201     177
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     317     378 
   Size:        317      61
 
MscI TGG'CCA

Cuts at:      0     322     378 
   Size:        322      56
 
StyI C'CwwG_G

Cuts at:      0     170     347     378 
   Size:        170     177      31

  Fragments arranged by size: 

                 177     170      31
 
TatI w'GTAC_w

Cuts at:      0     125     362     378 
   Size:        125     237      16

  Fragments arranged by size: 

                 237     125      16
 
XmnI GAAnn'nnTTC

Cuts at:      0     193     378 
   Size:        193     185
 

 Enzymes that do cut:

     AcuI     AfeI     ApoI     BanI    BanII Bme1580I     BpmI    BsiEI
 Bsp1286I    BspHI    BspMI    BsrDI    BsrFI    BsrGI    BstXI    BstYI
    BtgZI   DraIII     EaeI     EagI     EarI  Eco57MI    EcoRI    HaeII
    Hin4I   HincII     MmeI     MscI     StyI     TatI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     AscI
     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BbeI     BbsI
    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI    BglII     BlpI
    BmgBI     BmrI     BmtI     BplI   Bpu10I    BpuEI     BsaI    BsaAI
    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI  BsiHKAI
    BsiWI     BsmI    BsmBI    BspEI    BsrBI   BssHII    BssSI   BstAPI
    BstBI   BstEII  BstZ17I   Bsu36I     BtgI     BtsI     ClaI    CspCI
     DraI     DrdI     EciI  EcoICRI    EcoNI EcoO109I    EcoRV     FalI
     FseI     FspI    FspAI  HindIII     HpaI     KasI     KpnI     MfeI
     MluI     MslI   MspA1I     NaeI     NarI     NcoI     NdeI   NgoMIV
     NheI     NotI     NruI     NsiI     NspI     PacI     PasI     PciI
    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI
   PspOMI    PspXI     PsrI     PstI     PvuI    PvuII    RsrII     SacI
    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfcI
     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI
     SphI     SrfI     SspI     StuI     SwaI    TaqII     TstI  Tth111I
     XbaI     XcmI     XhoI     XmaI     ZraI