Restriction map for
pF1KB7065
(Linear) (Six-base) MAPSORT of: KIBB7065.seq Check: 9175 from: 1 to: 2349
KIBB7065.orf
With 182 enzymes: SgfI *
March 3, 2008 10:58 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 569 2129 2349
Size: 569 1560 220
Fragments arranged by size:
1560 569 220
AflIII A'CryG_T
Cuts at: 0 147 2349
Size: 147 2202
AleI CACnn'nnGTG
Cuts at: 0 1826 2349
Size: 1826 523
ApoI r'AATT_y
Cuts at: 0 120 259 267 371 387 455 791 875
Size: 120 139 8 104 16 68 336 84
Cuts at: 875 959 1127 1211 1295 1379 1463 1582 1741
Size: 84 168 84 84 84 84 119 159
Cuts at: 1741 1883 2051 2135 2303 2349
Size: 142 168 84 168 46
Fragments arranged by size:
336 168 168 168 159 142 139 120
119 104 84 84 84 84 84 84
84 68 46 16 8
BbsI GAAGACnn'nnnn_
Cuts at: 0 294 1346 2349
Size: 294 1052 1003
Fragments arranged by size:
1052 1003 294
Bme1580I G_kGCm'C
Cuts at: 0 170 2349
Size: 170 2179
BmrI ACTGGGnnnn_n'
Cuts at: 0 10 2349
Size: 10 2339
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 742 910 1750 2349
Size: 742 168 840 599
Fragments arranged by size:
840 742 599 168
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 485 653 737 905 1073 1241 1325 1409
Size: 485 168 84 168 168 168 84 84
Cuts at: 1409 1493 1661 1745 1829 1997 2081 2249 2333
Size: 84 168 84 84 168 84 168 84
Cuts at: 2333 2349
Size: 16
Fragments arranged by size:
485 168 168 168 168 168 168 168
84 84 84 84 84 84 84 84
16
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1932 2349
Size: 1932 417
BseYI C'CCAG_C
Cuts at: 0 799 2349
Size: 799 1550
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 932 1934 2024 2349
Size: 932 1002 90 325
Fragments arranged by size:
1002 932 325 90
Bsp1286I G_dGCh'C
Cuts at: 0 170 2349
Size: 170 2179
ClaI AT'CG_AT
Cuts at: 0 1266 2349
Size: 1266 1083
DraI TTT'AAA
Cuts at: 0 591 1599 2349
Size: 591 1008 750
Fragments arranged by size:
1008 750 591
EaeI y'GGCC_r
Cuts at: 0 49 2349
Size: 49 2300
EarI CTCTTCn'nnn_
Cuts at: 0 545 965 2349
Size: 545 420 1384
Fragments arranged by size:
1384 545 420
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 485 569 653 737 905 1073 1241 1325
Size: 485 84 84 84 168 168 168 84
Cuts at: 1325 1409 1493 1661 1745 1829 1997 2081 2129
Size: 84 84 168 84 84 168 84 48
Cuts at: 2129 2249 2333 2349
Size: 120 84 16
Fragments arranged by size:
485 168 168 168 168 168 120 84
84 84 84 84 84 84 84 84
84 48 16
EcoRI G'AATT_C
Cuts at: 0 371 387 455 791 1463 1883 2349
Size: 371 16 68 336 672 420 466
Fragments arranged by size:
672 466 420 371 336 68 16
HincII GTy'rAC
Cuts at: 0 192 2349
Size: 192 2157
HindIII A'AGCT_T
Cuts at: 0 752 920 1088 1172 1256 1424 1760 1844
Size: 752 168 168 84 84 168 336 84
Cuts at: 1844 2012 2096 2349
Size: 168 84 253
Fragments arranged by size:
752 336 253 168 168 168 168 84
84 84 84
HpaI GTT'AAC
Cuts at: 0 192 2349
Size: 192 2157
MscI TGG'CCA
Cuts at: 0 51 2349
Size: 51 2298
MslI CAynn'nnrTG
Cuts at: 0 152 1236 1826 2349
Size: 152 1084 590 523
Fragments arranged by size:
1084 590 523 152
NspI r_CATG'y
Cuts at: 0 151 2349
Size: 151 2198
PciI A'CATG_T
Cuts at: 0 147 2349
Size: 147 2202
PsiI TTA'TAA
Cuts at: 0 1850 2349
Size: 1850 499
PstI C_TGCA'G
Cuts at: 0 1140 2349
Size: 1140 1209
SfcI C'TryA_G
Cuts at: 0 1136 2349
Size: 1136 1213
SspI AAT'ATT
Cuts at: 0 224 2349
Size: 224 2125
StuI AGG'CCT
Cuts at: 0 586 598 1594 2349
Size: 586 12 996 755
Fragments arranged by size:
996 755 586 12
Enzymes that do cut:
AcuI AflIII AleI ApoI BbsI Bme1580I BmrI BplI
BpmI BseRI BseYI BsgI Bsp1286I ClaI DraI EaeI
EarI Eco57MI EcoRI HincII HindIII HpaI MscI MslI
NspI PciI PsiI PstI SfcI SspI StuI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AhdI AlfI AloI AlwNI ApaI ApaLI AscI AseI
AsiSI AvaI AvrII BaeI BamHI BanI BanII BbeI
BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI
BmgBI BmtI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BsiEI BsiHKAI BsiWI BsmI BsmBI BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI
BtgZI BtsI CspCI DraIII DrdI EagI EciI EcoICRI
EcoNI EcoO109I EcoRV FalI FseI FspI FspAI HaeII
Hin4I KasI KpnI MfeI MluI MmeI MspA1I NaeI
NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI
PacI PasI PflMI PfoI PmeI PmlI PpiI PpuMI
PshAI PspOMI PspXI PsrI PvuI PvuII RsrII SacI
SacII SalI SanDI SapI SbfI ScaI SexAI SfiI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SrfI StyI SwaI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI