Restriction map for
pF1KB7050
(Linear) (Six-base) MAPSORT of: KIBB7050.seq Check: 3351 from: 1 to: 1512
KIBB7050.orf
With 182 enzymes: SgfI *
March 3, 2008 10:58 ..
Acc65I G'GTAC_C
Cuts at: 0 108 1512
Size: 108 1404
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1473 1512
Size: 1473 39
AfeI AGC'GCT
Cuts at: 0 323 340 1512
Size: 323 17 1172
Fragments arranged by size:
1172 323 17
ApaLI G'TGCA_C
Cuts at: 0 375 1512
Size: 375 1137
AvaI C'yCGr_G
Cuts at: 0 204 232 458 483 1512
Size: 204 28 226 25 1029
Fragments arranged by size:
1029 226 204 28 25
AvrII C'CTAG_G
Cuts at: 0 741 1512
Size: 741 771
BanI G'GyrC_C
Cuts at: 0 108 589 1512
Size: 108 481 923
Fragments arranged by size:
923 481 108
BanII G_rGCy'C
Cuts at: 0 491 1512
Size: 491 1021
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 366 400 1512
Size: 366 34 1112
Fragments arranged by size:
1112 366 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 838 1512
Size: 838 674
BglI GCCn_nnn'nGGC
Cuts at: 0 418 1512
Size: 418 1094
Bme1580I G_kGCm'C
Cuts at: 0 379 1512
Size: 379 1133
BmrI ACTGGGnnnn_n'
Cuts at: 0 595 1512
Size: 595 917
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 259 866 1512
Size: 259 607 646
Fragments arranged by size:
646 607 259
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 310 615 954 1512
Size: 310 305 339 558
Fragments arranged by size:
558 339 310 305
BsaI GGTCTCn'nnnn_
Cuts at: 0 20 1512
Size: 20 1492
BsaHI Gr'CG_yC
Cuts at: 0 280 1512
Size: 280 1232
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 458 488 1512
Size: 458 30 1024
Fragments arranged by size:
1024 458 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 460 827 1220 1512
Size: 460 367 393 292
Fragments arranged by size:
460 393 367 292
BseYI C'CCAG_C
Cuts at: 0 132 151 579 1512
Size: 132 19 428 933
Fragments arranged by size:
933 428 132 19
BsiEI CG_ry'CG
Cuts at: 0 45 148 176 182 1512
Size: 45 103 28 6 1330
Fragments arranged by size:
1330 103 45 28 6
BsiHKAI G_wGCw'C
Cuts at: 0 379 484 1512
Size: 379 105 1028
Fragments arranged by size:
1028 379 105
BsmI GAATG_Cn'
Cuts at: 0 1040 1512
Size: 1040 472
Bsp1286I G_dGCh'C
Cuts at: 0 379 484 491 1512
Size: 379 105 7 1021
Fragments arranged by size:
1021 379 105 7
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 114 1512
Size: 114 1398
BsrBI CCG'CTC
Cuts at: 0 6 245 1512
Size: 6 239 1267
Fragments arranged by size:
1267 239 6
BsrFI r'CCGG_y
Cuts at: 0 105 225 1028 1512
Size: 105 120 803 484
Fragments arranged by size:
803 484 120 105
BstBI TT'CG_AA
Cuts at: 0 629 1512
Size: 629 883
BstYI r'GATC_y
Cuts at: 0 981 1512
Size: 981 531
Bsu36I CC'TnA_GG
Cuts at: 0 653 1512
Size: 653 859
BtgI C'CryG_G
Cuts at: 0 176 274 306 551 1512
Size: 176 98 32 245 961
Fragments arranged by size:
961 245 176 98 32
DraI TTT'AAA
Cuts at: 0 1091 1512
Size: 1091 421
DraIII CAC_nnn'GTG
Cuts at: 0 16 1512
Size: 16 1496
EaeI y'GGCC_r
Cuts at: 0 42 145 173 179 407 1399 1512
Size: 42 103 28 6 228 992 113
Fragments arranged by size:
992 228 113 103 42 28 6
EagI C'GGCC_G
Cuts at: 0 42 145 173 179 1512
Size: 42 103 28 6 1333
Fragments arranged by size:
1333 103 42 28 6
EarI CTCTTCn'nnn_
Cuts at: 0 860 1238 1329 1512
Size: 860 378 91 183
Fragments arranged by size:
860 378 183 91
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 74 1512
Size: 74 1438
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 259 866 1473 1512
Size: 259 607 607 39
Fragments arranged by size:
607 607 259 39
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1292 1512
Size: 1292 220
EcoO109I rG'GnC_Cy
Cuts at: 0 878 1512
Size: 878 634
EcoRV GAT'ATC
Cuts at: 0 993 1512
Size: 993 519
FspI TGC'GCA
Cuts at: 0 427 1512
Size: 427 1085
HaeII r_GCGC'y
Cuts at: 0 325 342 396 424 1512
Size: 325 17 54 28 1088
Fragments arranged by size:
1088 325 54 28 17
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 886 918 1283 1315 1512
Size: 886 32 365 32 197
Fragments arranged by size:
886 365 197 32 32
KpnI G_GTAC'C
Cuts at: 0 112 1512
Size: 112 1400
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 836 982 1512
Size: 836 146 530
Fragments arranged by size:
836 530 146
MspA1I CmG'CkG
Cuts at: 0 172 178 193 276 296 350 1512
Size: 172 6 15 83 20 54 1162
Fragments arranged by size:
1162 172 83 54 20 15 6
NaeI GCC'GGC
Cuts at: 0 227 1030 1512
Size: 227 803 482
Fragments arranged by size:
803 482 227
NcoI C'CATG_G
Cuts at: 0 306 1512
Size: 306 1206
NgoMIV G'CCGG_C
Cuts at: 0 225 1028 1512
Size: 225 803 484
Fragments arranged by size:
803 484 225
NotI GC'GGCC_GC
Cuts at: 0 145 173 179 1512
Size: 145 28 6 1333
Fragments arranged by size:
1333 145 28 6
PasI CC'CwG_GG
Cuts at: 0 61 1512
Size: 61 1451
PflMI CCAn_nnn'nTGG
Cuts at: 0 312 1512
Size: 312 1200
PfoI T'CCnGG_A
Cuts at: 0 843 1512
Size: 843 669
PpuMI rG'GwC_Cy
Cuts at: 0 878 1512
Size: 878 634
PstI C_TGCA'G
Cuts at: 0 1332 1512
Size: 1332 180
SacII CC_GC'GG
Cuts at: 0 179 277 1512
Size: 179 98 1235
Fragments arranged by size:
1235 179 98
SfcI C'TryA_G
Cuts at: 0 466 1328 1512
Size: 466 862 184
Fragments arranged by size:
862 466 184
SmaI CCC'GGG
Cuts at: 0 234 460 1512
Size: 234 226 1052
Fragments arranged by size:
1052 234 226
SmlI C'TyrA_G
Cuts at: 0 325 594 969 1512
Size: 325 269 375 543
Fragments arranged by size:
543 375 325 269
StyI C'CwwG_G
Cuts at: 0 306 741 944 1512
Size: 306 435 203 568
Fragments arranged by size:
568 435 306 203
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 44 577 1512
Size: 44 533 935
Fragments arranged by size:
935 533 44
TatI w'GTAC_w
Cuts at: 0 1484 1512
Size: 1484 28
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 349 381 1512
Size: 349 32 1131
Fragments arranged by size:
1131 349 32
Tth111I GACn'n_nGTC
Cuts at: 0 963 1512
Size: 963 549
XmaI C'CCGG_G
Cuts at: 0 232 458 1512
Size: 232 226 1054
Fragments arranged by size:
1054 232 226
XmnI GAAnn'nnTTC
Cuts at: 0 1040 1512
Size: 1040 472
Enzymes that do cut:
Acc65I AcuI AfeI ApaLI AvaI AvrII BanI BanII
BcgI BciVI BglI Bme1580I BmrI BpmI BpuEI BsaI
BsaHI BsaXI BseRI BseYI BsiEI BsiHKAI BsmI Bsp1286I
BspMI BsrBI BsrFI BstBI BstYI Bsu36I BtgI DraI
DraIII EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I
EcoRV FspI HaeII Hin4I KpnI MmeI MspA1I NaeI
NcoI NgoMIV NotI PasI PflMI PfoI PpuMI PstI
SacII SfcI SmaI SmlI StyI TaqII TatI TstI
Tth111I XmaI XmnI
Enzymes that do not cut:
AarI AatII AccI AclI AflII AflIII AgeI AhdI
AleI AlfI AloI AlwNI ApaI ApoI AscI AseI
AsiSI BaeI BamHI BbeI BbsI BbvCI BclI BfrBI
BglII BlpI BmgBI BmtI BplI Bpu10I BsaAI BsaBI
BsaWI BsgI BsiWI BsmBI BspEI BspHI BsrDI BsrGI
BssHII BssSI BstAPI BstEII BstXI BstZ17I BtgZI BtsI
ClaI CspCI DrdI EcoICRI EcoRI FalI FseI FspAI
HincII HindIII HpaI KasI MfeI MluI MscI MslI
NarI NdeI NheI NruI NsiI NspI PacI PciI
PmeI PmlI PpiI PshAI PsiI PspOMI PspXI PsrI
PvuI PvuII RsrII SacI SalI SanDI SapI SbfI
ScaI SexAI SfiI SfoI SgfI SgrAI SnaBI SpeI
SphI SrfI SspI StuI SwaI XbaI XcmI XhoI
ZraI