Restriction map for
pF1KB7049
(Linear) (Six-base) MAPSORT of: KIBB7049.seq Check: 4425 from: 1 to: 1476
KIBB7049.orf
With 182 enzymes: SgfI *
March 3, 2008 10:58 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 67 1346 1415 1445 1476
Size: 67 1279 69 30 31
Fragments arranged by size:
1279 69 67 31 30
AhdI GACnn_n'nnGTC
Cuts at: 0 810 1460 1476
Size: 810 650 16
Fragments arranged by size:
810 650 16
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 833 1476
Size: 833 643
ApoI r'AATT_y
Cuts at: 0 1249 1476
Size: 1249 227
AvaI C'yCGr_G
Cuts at: 0 1277 1476
Size: 1277 199
BamHI G'GATC_C
Cuts at: 0 226 1413 1476
Size: 226 1187 63
Fragments arranged by size:
1187 226 63
BanI G'GyrC_C
Cuts at: 0 15 1476
Size: 15 1461
BanII G_rGCy'C
Cuts at: 0 1013 1078 1476
Size: 1013 65 398
Fragments arranged by size:
1013 398 65
BglII A'GATC_T
Cuts at: 0 1390 1476
Size: 1390 86
Bme1580I G_kGCm'C
Cuts at: 0 20 508 1476
Size: 20 488 968
Fragments arranged by size:
968 488 20
BmrI ACTGGGnnnn_n'
Cuts at: 0 31 721 950 1158 1476
Size: 31 690 229 208 318
Fragments arranged by size:
690 318 229 208 31
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 622 1476
Size: 622 854
BsaI GGTCTCn'nnnn_
Cuts at: 0 1468 1476
Size: 1468 8
BsaAI yAC'GTr
Cuts at: 0 460 1476
Size: 460 1016
BsaBI GATnn'nnATC
Cuts at: 0 599 1476
Size: 599 877
BsaWI w'CCGG_w
Cuts at: 0 1459 1476
Size: 1459 17
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 763 793 1476
Size: 763 30 683
Fragments arranged by size:
763 683 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1456 1476
Size: 1456 20
BseYI C'CCAG_C
Cuts at: 0 1438 1476
Size: 1438 38
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 494 1476
Size: 494 982
BsiHKAI G_wGCw'C
Cuts at: 0 1013 1476
Size: 1013 463
Bsp1286I G_dGCh'C
Cuts at: 0 20 508 1013 1078 1476
Size: 20 488 505 65 398
Fragments arranged by size:
505 488 398 65 20
BspHI T'CATG_A
Cuts at: 0 591 1005 1476
Size: 591 414 471
Fragments arranged by size:
591 471 414
BsrDI GCAATG_nn'
Cuts at: 0 1235 1476
Size: 1235 241
BsrGI T'GTAC_A
Cuts at: 0 498 561 1476
Size: 498 63 915
Fragments arranged by size:
915 498 63
BstYI r'GATC_y
Cuts at: 0 226 1390 1413 1476
Size: 226 1164 23 63
Fragments arranged by size:
1164 226 63 23
DraIII CAC_nnn'GTG
Cuts at: 0 958 1476
Size: 958 518
DrdI GACnn_nn'nnGTC
Cuts at: 0 81 1476
Size: 81 1395
EaeI y'GGCC_r
Cuts at: 0 883 1024 1062 1476
Size: 883 141 38 414
Fragments arranged by size:
883 414 141 38
EarI CTCTTCn'nnn_
Cuts at: 0 36 566 1304 1476
Size: 36 530 738 172
Fragments arranged by size:
738 530 172 36
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 639 1476
Size: 639 837
EcoICRI GAG'CTC
Cuts at: 0 1011 1476
Size: 1011 465
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 67 1346 1415 1445 1476
Size: 67 1279 69 30 31
Fragments arranged by size:
1279 69 67 31 30
EcoO109I rG'GnC_Cy
Cuts at: 0 67 1476
Size: 67 1409
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 72 1476
Size: 72 1404
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 478 510 763 795 796 828 1446 1476
Size: 478 32 253 32 1 32 618 30
Fragments arranged by size:
618 478 253 32 32 32 30 1
HindIII A'AGCT_T
Cuts at: 0 1427 1476
Size: 1427 49
MscI TGG'CCA
Cuts at: 0 1026 1064 1476
Size: 1026 38 412
Fragments arranged by size:
1026 412 38
MslI CAynn'nnrTG
Cuts at: 0 1174 1476
Size: 1174 302
NspI r_CATG'y
Cuts at: 0 636 788 810 1476
Size: 636 152 22 666
Fragments arranged by size:
666 636 152 22
PflMI CCAn_nnn'nTGG
Cuts at: 0 146 912 1476
Size: 146 766 564
Fragments arranged by size:
766 564 146
PmlI CAC'GTG
Cuts at: 0 460 1476
Size: 460 1016
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 820 852 1476
Size: 820 32 624
Fragments arranged by size:
820 624 32
SacI G_AGCT'C
Cuts at: 0 1013 1476
Size: 1013 463
SapI GCTCTTCn'nnn_
Cuts at: 0 1304 1476
Size: 1304 172
ScaI AGT'ACT
Cuts at: 0 403 1476
Size: 403 1073
SexAI A'CCwGG_T
Cuts at: 0 995 1476
Size: 995 481
SfcI C'TryA_G
Cuts at: 0 180 1476
Size: 180 1296
SmaI CCC'GGG
Cuts at: 0 1279 1476
Size: 1279 197
SmlI C'TyrA_G
Cuts at: 0 637 1476
Size: 637 839
SphI G_CATG'C
Cuts at: 0 636 1476
Size: 636 840
StuI AGG'CCT
Cuts at: 0 743 1160 1476
Size: 743 417 316
Fragments arranged by size:
743 417 316
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 969 1476
Size: 969 507
TatI w'GTAC_w
Cuts at: 0 401 498 561 1476
Size: 401 97 63 915
Fragments arranged by size:
915 401 97 63
XmaI C'CCGG_G
Cuts at: 0 1277 1476
Size: 1277 199
XmnI GAAnn'nnTTC
Cuts at: 0 332 1476
Size: 332 1144
Enzymes that do cut:
AcuI AhdI AlfI ApoI AvaI BamHI BanI BanII
BglII Bme1580I BmrI BpuEI BsaI BsaAI BsaBI BsaWI
BsaXI BseRI BseYI BsgI BsiHKAI Bsp1286I BspHI BsrDI
BsrGI BstYI DraIII DrdI EaeI EarI EciI EcoICRI
Eco57MI EcoO109I FalI Hin4I HindIII MscI MslI NspI
PflMI PmlI PpiI SacI SapI ScaI SexAI SfcI
SmaI SmlI SphI StuI TaqII TatI XmaI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AleI AloI AlwNI ApaI ApaLI AscI AseI
AsiSI AvrII BaeI BbeI BbsI BbvCI BcgI BciVI
BclI BfrBI BglI BlpI BmgBI BmtI BplI BpmI
Bpu10I BsaHI BsiEI BsiWI BsmI BsmBI BspEI BspMI
BsrBI BsrFI BssHII BssSI BstAPI BstBI BstEII BstXI
BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
EagI EcoNI EcoRI EcoRV FseI FspI FspAI HaeII
HincII HpaI KasI KpnI MfeI MluI MmeI MspA1I
NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI
NsiI PacI PasI PciI PfoI PmeI PpuMI PshAI
PsiI PspOMI PspXI PsrI PstI PvuI PvuII RsrII
SacII SalI SanDI SbfI SfiI SfoI SgfI SgrAI
SnaBI SpeI SrfI SspI StyI SwaI TstI Tth111I
XbaI XcmI XhoI ZraI