Restriction map for
pF1KB7047
(Linear) (Six-base) MAPSORT of: KIBB7047.seq Check: 1544 from: 1 to: 1470
KIBB7047.orf
With 182 enzymes: SgfI *
March 3, 2008 10:58 ..
AccI GT'mk_AC
Cuts at: 0 308 1470
Size: 308 1162
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 251 460 692 914 1084 1142 1334 1470
Size: 251 209 232 222 170 58 192 136
Fragments arranged by size:
251 232 222 209 192 170 136 58
AfeI AGC'GCT
Cuts at: 0 1087 1470
Size: 1087 383
AleI CACnn'nnGTG
Cuts at: 0 1132 1470
Size: 1132 338
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 269 1470
Size: 269 1201
AlwNI CAG_nnn'CTG
Cuts at: 0 1381 1470
Size: 1381 89
ApoI r'AATT_y
Cuts at: 0 18 127 376 511 745 1470
Size: 18 109 249 135 234 725
Fragments arranged by size:
725 249 234 135 109 18
AseI AT'TA_AT
Cuts at: 0 1267 1470
Size: 1267 203
BanII G_rGCy'C
Cuts at: 0 1445 1470
Size: 1445 25
BbsI GAAGACnn'nnnn_
Cuts at: 0 86 1130 1470
Size: 86 1044 340
Fragments arranged by size:
1044 340 86
BfrBI ATG'CAT
Cuts at: 0 991 1470
Size: 991 479
BglII A'GATC_T
Cuts at: 0 1287 1470
Size: 1287 183
BmrI ACTGGGnnnn_n'
Cuts at: 0 1333 1470
Size: 1333 137
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1462 1464 1470
Size: 1462 2 6
Fragments arranged by size:
1462 6 2
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 46 1036 1413 1470
Size: 46 990 377 57
Fragments arranged by size:
990 377 57 46
Bpu10I CC'TnA_GC
Cuts at: 0 1336 1470
Size: 1336 134
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 75 985 1408 1470
Size: 75 910 423 62
Fragments arranged by size:
910 423 75 62
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 507 1435 1470
Size: 507 928 35
Fragments arranged by size:
928 507 35
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 758 1470
Size: 758 712
BsiHKAI G_wGCw'C
Cuts at: 0 1445 1470
Size: 1445 25
BsmI GAATG_Cn'
Cuts at: 0 220 993 1470
Size: 220 773 477
Fragments arranged by size:
773 477 220
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1012 1470
Size: 1012 458
Bsp1286I G_dGCh'C
Cuts at: 0 1445 1470
Size: 1445 25
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 376 1470
Size: 376 1094
BsrGI T'GTAC_A
Cuts at: 0 158 1470
Size: 158 1312
BstBI TT'CG_AA
Cuts at: 0 952 1470
Size: 952 518
BstEII G'GTnAC_C
Cuts at: 0 9 763 1470
Size: 9 754 707
Fragments arranged by size:
754 707 9
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1250 1470
Size: 1250 220
BstYI r'GATC_y
Cuts at: 0 96 1287 1470
Size: 96 1191 183
Fragments arranged by size:
1191 183 96
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 550 1470
Size: 550 920
BtsI GCAGTG_nn'
Cuts at: 0 741 1127 1470
Size: 741 386 343
Fragments arranged by size:
741 386 343
DraI TTT'AAA
Cuts at: 0 134 1470
Size: 134 1336
EarI CTCTTCn'nnn_
Cuts at: 0 227 1181 1470
Size: 227 954 289
Fragments arranged by size:
954 289 227
EcoICRI GAG'CTC
Cuts at: 0 1443 1470
Size: 1443 27
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 46 251 460 692 914 1036 1084 1142
Size: 46 205 209 232 222 122 48 58
Cuts at: 1142 1334 1413 1470
Size: 192 79 57
Fragments arranged by size:
232 222 209 205 192 122 79 58
57 48 46
EcoRI G'AATT_C
Cuts at: 0 18 511 1470
Size: 18 493 959
Fragments arranged by size:
959 493 18
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 651 1470
Size: 651 819
HaeII r_GCGC'y
Cuts at: 0 1089 1470
Size: 1089 381
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 73 105 528 560 1141 1173 1470
Size: 73 32 423 32 581 32 297
Fragments arranged by size:
581 423 297 73 32 32 32
MfeI C'AATT_G
Cuts at: 0 530 1470
Size: 530 940
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 782 1470
Size: 782 688
MslI CAynn'nnrTG
Cuts at: 0 188 1132 1470
Size: 188 944 338
Fragments arranged by size:
944 338 188
MspA1I CmG'CkG
Cuts at: 0 45 1470
Size: 45 1425
NsiI A_TGCA'T
Cuts at: 0 993 1470
Size: 993 477
NspI r_CATG'y
Cuts at: 0 256 1470
Size: 256 1214
PflMI CCAn_nnn'nTGG
Cuts at: 0 820 1470
Size: 820 650
PfoI T'CCnGG_A
Cuts at: 0 1013 1470
Size: 1013 457
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 553 585 1470
Size: 553 32 885
Fragments arranged by size:
885 553 32
PstI C_TGCA'G
Cuts at: 0 653 1470
Size: 653 817
PvuII CAG'CTG
Cuts at: 0 45 1470
Size: 45 1425
SacI G_AGCT'C
Cuts at: 0 1445 1470
Size: 1445 25
SfcI C'TryA_G
Cuts at: 0 649 1470
Size: 649 821
SmlI C'TyrA_G
Cuts at: 0 90 964 1387 1470
Size: 90 874 423 83
Fragments arranged by size:
874 423 90 83
StyI C'CwwG_G
Cuts at: 0 698 1470
Size: 698 772
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 572 833 1470
Size: 572 261 637
Fragments arranged by size:
637 572 261
TatI w'GTAC_w
Cuts at: 0 158 1470
Size: 158 1312
Tth111I GACn'n_nGTC
Cuts at: 0 85 1470
Size: 85 1385
XbaI T'CTAG_A
Cuts at: 0 99 1470
Size: 99 1371
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 21 1470
Size: 21 1449
XmnI GAAnn'nnTTC
Cuts at: 0 70 568 788 1470
Size: 70 498 220 682
Fragments arranged by size:
682 498 220 70
Enzymes that do cut:
AccI AcuI AfeI AleI AlfI AlwNI ApoI AseI
BanII BbsI BfrBI BglII BmrI BplI BpmI Bpu10I
BpuEI BseRI BsgI BsiHKAI BsmI BsmBI Bsp1286I BspMI
BsrGI BstBI BstEII BstXI BstYI BtgZI BtsI DraI
EarI EcoICRI Eco57MI EcoRI FalI HaeII Hin4I MfeI
MmeI MslI MspA1I NsiI NspI PflMI PfoI PpiI
PstI PvuII SacI SfcI SmlI StyI TaqII TatI
Tth111I XbaI XcmI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AflII AflIII AgeI AhdI
AloI ApaI ApaLI AscI AsiSI AvaI AvrII BaeI
BamHI BanI BbeI BbvCI BcgI BciVI BclI BglI
BlpI Bme1580I BmgBI BmtI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseYI BsiEI BsiWI BspEI BspHI BsrBI
BsrDI BsrFI BssHII BssSI BstAPI BstZ17I Bsu36I BtgI
ClaI CspCI DraIII DrdI EaeI EagI EciI EcoNI
EcoO109I EcoRV FseI FspI FspAI HincII HindIII HpaI
KasI KpnI MluI MscI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI PacI PasI PciI PmeI
PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI PvuI
RsrII SacII SalI SanDI SapI SbfI ScaI SexAI
SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI
SrfI SspI StuI SwaI TstI XhoI XmaI ZraI