Restriction map for
pF1KB7039
(Linear) (Six-base) MAPSORT of: KIBB7039.seq Check: 6411 from: 1 to: 1374
KIBB7039.orf
With 182 enzymes: SgfI *
March 3, 2008 10:58 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 5 1374
Size: 5 1369
AflIII A'CryG_T
Cuts at: 0 1202 1374
Size: 1202 172
AhdI GACnn_n'nnGTC
Cuts at: 0 1118 1374
Size: 1118 256
AlwNI CAG_nnn'CTG
Cuts at: 0 339 942 1374
Size: 339 603 432
Fragments arranged by size:
603 432 339
ApaLI G'TGCA_C
Cuts at: 0 222 1374
Size: 222 1152
AvaI C'yCGr_G
Cuts at: 0 760 1374
Size: 760 614
BanII G_rGCy'C
Cuts at: 0 245 1374
Size: 245 1129
BbsI GAAGACnn'nnnn_
Cuts at: 0 461 1374
Size: 461 913
BfrBI ATG'CAT
Cuts at: 0 801 1374
Size: 801 573
BlpI GC'TnA_GC
Cuts at: 0 88 244 1374
Size: 88 156 1130
Fragments arranged by size:
1130 156 88
Bme1580I G_kGCm'C
Cuts at: 0 226 761 1374
Size: 226 535 613
Fragments arranged by size:
613 535 226
BmgBI CAC'GTC
Cuts at: 0 867 1230 1374
Size: 867 363 144
Fragments arranged by size:
867 363 144
BmrI ACTGGGnnnn_n'
Cuts at: 0 1036 1374
Size: 1036 338
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 81 240 281 361 760 793 892 1374
Size: 81 159 41 80 399 33 99 482
Fragments arranged by size:
482 399 159 99 81 80 41 33
Bpu10I CC'TnA_GC
Cuts at: 0 181 1059 1374
Size: 181 878 315
Fragments arranged by size:
878 315 181
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 382 1374
Size: 382 992
BsaI GGTCTCn'nnnn_
Cuts at: 0 57 655 736 1261 1374
Size: 57 598 81 525 113
Fragments arranged by size:
598 525 113 81 57
BsaAI yAC'GTr
Cuts at: 0 900 1203 1374
Size: 900 303 171
Fragments arranged by size:
900 303 171
BsaWI w'CCGG_w
Cuts at: 0 192 1374
Size: 192 1182
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 133 163 1171 1201 1374
Size: 133 30 1008 30 173
Fragments arranged by size:
1008 173 133 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 157 673 790 1374
Size: 157 516 117 584
Fragments arranged by size:
584 516 157 117
BseYI C'CCAG_C
Cuts at: 0 99 129 891 1374
Size: 99 30 762 483
Fragments arranged by size:
762 483 99 30
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1021 1374
Size: 1021 353
BsiEI CG_ry'CG
Cuts at: 0 543 1374
Size: 543 831
BsiHKAI G_wGCw'C
Cuts at: 0 164 226 245 1374
Size: 164 62 19 1129
Fragments arranged by size:
1129 164 62 19
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1348 1374
Size: 1348 26
Bsp1286I G_dGCh'C
Cuts at: 0 164 226 245 761 1374
Size: 164 62 19 516 613
Fragments arranged by size:
613 516 164 62 19
BspHI T'CATG_A
Cuts at: 0 1127 1374
Size: 1127 247
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 185 911 1043 1374
Size: 185 726 132 331
Fragments arranged by size:
726 331 185 132
BsrFI r'CCGG_y
Cuts at: 0 916 1374
Size: 916 458
BsrGI T'GTAC_A
Cuts at: 0 485 1002 1374
Size: 485 517 372
Fragments arranged by size:
517 485 372
BstYI r'GATC_y
Cuts at: 0 875 1374
Size: 875 499
BtgI C'CryG_G
Cuts at: 0 236 908 1374
Size: 236 672 466
Fragments arranged by size:
672 466 236
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1236 1374
Size: 1236 138
BtsI GCAGTG_nn'
Cuts at: 0 154 547 1315 1374
Size: 154 393 768 59
Fragments arranged by size:
768 393 154 59
DraIII CAC_nnn'GTG
Cuts at: 0 221 690 1044 1374
Size: 221 469 354 330
Fragments arranged by size:
469 354 330 221
EaeI y'GGCC_r
Cuts at: 0 278 692 1374
Size: 278 414 682
Fragments arranged by size:
682 414 278
EarI CTCTTCn'nnn_
Cuts at: 0 388 557 760 1374
Size: 388 169 203 614
Fragments arranged by size:
614 388 203 169
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 490 1374
Size: 490 884
EcoICRI GAG'CTC
Cuts at: 0 243 1374
Size: 243 1131
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 5 81 240 281 361 760 793 892
Size: 5 76 159 41 80 399 33 99
Cuts at: 892 1374
Size: 482
Fragments arranged by size:
482 399 159 99 80 76 41 33
5
EcoNI CCTnn'n_nnAGG
Cuts at: 0 331 1144 1374
Size: 331 813 230
Fragments arranged by size:
813 331 230
EcoO109I rG'GnC_Cy
Cuts at: 0 104 336 939 1112 1374
Size: 104 232 603 173 262
Fragments arranged by size:
603 262 232 173 104
FspI TGC'GCA
Cuts at: 0 646 1374
Size: 646 728
HaeII r_GCGC'y
Cuts at: 0 354 1374
Size: 354 1020
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 537 569 941 973 1104 1136 1171 1203
Size: 537 32 372 32 131 32 35 32
Cuts at: 1203 1374
Size: 171
Fragments arranged by size:
537 372 171 131 35 32 32 32
32
HincII GTy'rAC
Cuts at: 0 534 1374
Size: 534 840
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 290 927 1187 1214 1374
Size: 290 637 260 27 160
Fragments arranged by size:
637 290 260 160 27
MscI TGG'CCA
Cuts at: 0 280 694 1374
Size: 280 414 680
Fragments arranged by size:
680 414 280
MslI CAynn'nnrTG
Cuts at: 0 13 796 1141 1374
Size: 13 783 345 233
Fragments arranged by size:
783 345 233 13
MspA1I CmG'CkG
Cuts at: 0 129 301 1185 1374
Size: 129 172 884 189
Fragments arranged by size:
884 189 172 129
NaeI GCC'GGC
Cuts at: 0 918 1374
Size: 918 456
NgoMIV G'CCGG_C
Cuts at: 0 916 1374
Size: 916 458
NsiI A_TGCA'T
Cuts at: 0 803 1374
Size: 803 571
NspI r_CATG'y
Cuts at: 0 211 801 805 1374
Size: 211 590 4 569
Fragments arranged by size:
590 569 211 4
PflMI CCAn_nnn'nTGG
Cuts at: 0 339 690 1206 1374
Size: 339 351 516 168
Fragments arranged by size:
516 351 339 168
PfoI T'CCnGG_A
Cuts at: 0 737 1374
Size: 737 637
PmlI CAC'GTG
Cuts at: 0 1203 1374
Size: 1203 171
PpuMI rG'GwC_Cy
Cuts at: 0 104 939 1112 1374
Size: 104 835 173 262
Fragments arranged by size:
835 262 173 104
PsiI TTA'TAA
Cuts at: 0 1070 1374
Size: 1070 304
PvuI CG_AT'CG
Cuts at: 0 543 1374
Size: 543 831
RsrII CG'GwC_CG
Cuts at: 0 1187 1374
Size: 1187 187
SacI G_AGCT'C
Cuts at: 0 245 1374
Size: 245 1129
SanDI GG'GwC_CC
Cuts at: 0 104 1374
Size: 104 1270
SapI GCTCTTCn'nnn_
Cuts at: 0 388 760 1374
Size: 388 372 614
Fragments arranged by size:
614 388 372
ScaI AGT'ACT
Cuts at: 0 1306 1374
Size: 1306 68
SmaI CCC'GGG
Cuts at: 0 762 1374
Size: 762 612
SmlI C'TyrA_G
Cuts at: 0 397 1374
Size: 397 977
SphI G_CATG'C
Cuts at: 0 211 801 805 1374
Size: 211 590 4 569
Fragments arranged by size:
590 569 211 4
StuI AGG'CCT
Cuts at: 0 170 292 1236 1374
Size: 170 122 944 138
Fragments arranged by size:
944 170 138 122
StyI C'CwwG_G
Cuts at: 0 287 626 1374
Size: 287 339 748
Fragments arranged by size:
748 339 287
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 45 66 1055 1374
Size: 45 21 989 319
Fragments arranged by size:
989 319 45 21
TatI w'GTAC_w
Cuts at: 0 485 815 1002 1261 1304 1374
Size: 485 330 187 259 43 70
Fragments arranged by size:
485 330 259 187 70 43
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 133 165 1374
Size: 133 32 1209
Fragments arranged by size:
1209 133 32
XmaI C'CCGG_G
Cuts at: 0 760 1374
Size: 760 614
Enzymes that do cut:
AcuI AflIII AhdI AlwNI ApaLI AvaI BanII BbsI
BfrBI BlpI Bme1580I BmgBI BmrI BpmI Bpu10I BpuEI
BsaI BsaAI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmBI Bsp1286I BspHI BspMI BsrFI BsrGI BstYI
BtgI BtgZI BtsI DraIII EaeI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I FspI HaeII Hin4I HincII MmeI
MscI MslI MspA1I NaeI NgoMIV NsiI NspI PflMI
PfoI PmlI PpuMI PsiI PvuI RsrII SacI SanDI
SapI ScaI SmaI SmlI SphI StuI StyI TaqII
TatI TstI XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AleI AlfI AloI ApaI ApoI AscI AseI AsiSI
AvrII BaeI BamHI BanI BbeI BbvCI BcgI BciVI
BclI BglI BglII BmtI BplI BsaBI BsaHI BsiWI
BsmI BspEI BsrBI BsrDI BssHII BssSI BstAPI BstBI
BstEII BstXI BstZ17I Bsu36I ClaI CspCI DraI DrdI
EagI EcoRI EcoRV FalI FseI FspAI HindIII HpaI
KasI KpnI MfeI MluI NarI NcoI NdeI NheI
NotI NruI PacI PasI PciI PmeI PpiI PshAI
PspOMI PspXI PsrI PstI PvuII SacII SalI SbfI
SexAI SfcI SfiI SfoI SgfI SgrAI SnaBI SpeI
SrfI SspI SwaI Tth111I XbaI XcmI XhoI XmnI
ZraI